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The map label for this gene is intE [H]

Identifier: 85058907

GI number: 85058907

Start: 1520735

End: 1521802

Strand: Reverse

Name: intE [H]

Synonym: SG0929

Alternate gene names: 85058907

Gene position: 1521802-1520735 (Counterclockwise)

Preceding gene: 85058908

Following gene: 85058901

Centisome position: 36.48

GC content: 48.88

Gene sequence:

>1068_bases
ATGGCACGCCGGAGAAGCCATAAAAACCGCGATTTGCCGCCAAACCTGCAACCCATAAATGGCGGCTATTTTTGCTATCG
CGACCCACGAACAGGAAAAGAATACGGCCTCGGACGCAATCGGCGGGAGGCTATTACGCAAGCCATAGCCGCTAATATGG
CGATTTATGGCTCATCAAAACAATCACCACTCGTTGACCGCATTAGCGGCACACAGGCGACCACAGTTAGCGAATGGACT
ACACGTTATTTGACAATACTCGAAGGTCGGAAGCTAAAACCAAAGACGATGACTGAGCATCGGAAATATTTGAAAGCTAT
CAATGAACATCTGGGCGAATTTGCTATTCAGCAGGTGCAAACAAAAGATGTTGCTGCCTTCCTGAATCTGTGGGTCAAAG
CCGGGAAAGAAACGATGGCGAATAAATTGAGAAAGGAAGTTATTGATTTATTCCGCGAGGCCATTGCAGATGGGATAGTA
TCGCACAACCCCGCCGAGGCCACACGGAACCAGCGTGTAGAAATCCAGCGTGAGCGTATAACACTTGAGGAATGGAAGGT
CATCCGCAAGATAGCTGAGAAATTACCTGCATGGGTTGGCTCCTGTCTTGACCTTGCCCTGGTTACAGGTCAACGACTGG
GCGATATACAGGCGCTTCTCTGGGAGAATTTCAAAGACGGACGCCTTTACATCAAGCAAGCGAAAACAGGCATGATGCTG
GCATTCTCCGCGGTAACACGTATTGATTGCCTCAACTTATGCCTTGCTGATGTACTGAGCCAGTTTAAGGCATTAAATAG
CACTGGGTCACATATTGTTGCAGACTCCAAACATGAACAATTTGCCGCAAAAACGATATCCGCCGCCTTTTTGAAGGCGC
GTCGCGTATCTGGATTGACGTGGCAAGGTGACCCGCCTTCATTCCATGAAATCAGAAGTTTGGCGGCACGACTCTACACC
GATGAGAAAGGGAGGGATTATGCGCAAAAACTGCTAGGACATAAGTCATCCGAGATGACCGATAAGTACCGCGATGTAAG
AGGGTCGGAGTGGGCCGAGATTGAATAG

Upstream 100 bases:

>100_bases
CGAGAATATTTAGTTGACGAAAACGCCGAGAAGCTTACCCGAAACAATACCTTCAACCCATCCAGAACTGTTCACCCCGC
ATTACTATCGAGGATAAAAC

Downstream 100 bases:

>100_bases
TTTCGATCGAATTTCGAGTAATTTCGATCGATCACTTGTAACTATTTGAATTTAAATGCCATAATCTTAATGCAGCTGCG
CTTCGAGCAGCGCCATTAAT

Product: phage integrase

Products: NA

Alternate protein names: Int(Lambda); Prophage e14 integrase [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MARRRSHKNRDLPPNLQPINGGYFCYRDPRTGKEYGLGRNRREAITQAIAANMAIYGSSKQSPLVDRISGTQATTVSEWT
TRYLTILEGRKLKPKTMTEHRKYLKAINEHLGEFAIQQVQTKDVAAFLNLWVKAGKETMANKLRKEVIDLFREAIADGIV
SHNPAEATRNQRVEIQRERITLEEWKVIRKIAEKLPAWVGSCLDLALVTGQRLGDIQALLWENFKDGRLYIKQAKTGMML
AFSAVTRIDCLNLCLADVLSQFKALNSTGSHIVADSKHEQFAAKTISAAFLKARRVSGLTWQGDPPSFHEIRSLAARLYT
DEKGRDYAQKLLGHKSSEMTDKYRDVRGSEWAEIE

Sequences:

>Translated_355_residues
MARRRSHKNRDLPPNLQPINGGYFCYRDPRTGKEYGLGRNRREAITQAIAANMAIYGSSKQSPLVDRISGTQATTVSEWT
TRYLTILEGRKLKPKTMTEHRKYLKAINEHLGEFAIQQVQTKDVAAFLNLWVKAGKETMANKLRKEVIDLFREAIADGIV
SHNPAEATRNQRVEIQRERITLEEWKVIRKIAEKLPAWVGSCLDLALVTGQRLGDIQALLWENFKDGRLYIKQAKTGMML
AFSAVTRIDCLNLCLADVLSQFKALNSTGSHIVADSKHEQFAAKTISAAFLKARRVSGLTWQGDPPSFHEIRSLAARLYT
DEKGRDYAQKLLGHKSSEMTDKYRDVRGSEWAEIE
>Mature_354_residues
ARRRSHKNRDLPPNLQPINGGYFCYRDPRTGKEYGLGRNRREAITQAIAANMAIYGSSKQSPLVDRISGTQATTVSEWTT
RYLTILEGRKLKPKTMTEHRKYLKAINEHLGEFAIQQVQTKDVAAFLNLWVKAGKETMANKLRKEVIDLFREAIADGIVS
HNPAEATRNQRVEIQRERITLEEWKVIRKIAEKLPAWVGSCLDLALVTGQRLGDIQALLWENFKDGRLYIKQAKTGMMLA
FSAVTRIDCLNLCLADVLSQFKALNSTGSHIVADSKHEQFAAKTISAAFLKARRVSGLTWQGDPPSFHEIRSLAARLYTD
EKGRDYAQKLLGHKSSEMTDKYRDVRGSEWAEIE

Specific function: Integrase from the cryptic lambdoic prophage e14. Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the h

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1787386, Length=372, Percent_Identity=36.5591397849462, Blast_Score=195, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016177
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR015094
- InterPro:   IPR023109
- InterPro:   IPR013087 [H]

Pfam domain/function: PF09003 Phage_integ_N; PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 40263; Mature: 40131

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRRSHKNRDLPPNLQPINGGYFCYRDPRTGKEYGLGRNRREAITQAIAANMAIYGSSK
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHEECCCC
QSPLVDRISGTQATTVSEWTTRYLTILEGRKLKPKTMTEHRKYLKAINEHLGEFAIQQVQ
CCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TKDVAAFLNLWVKAGKETMANKLRKEVIDLFREAIADGIVSHNPAEATRNQRVEIQRERI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHH
TLEEWKVIRKIAEKLPAWVGSCLDLALVTGQRLGDIQALLWENFKDGRLYIKQAKTGMML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCHHH
AFSAVTRIDCLNLCLADVLSQFKALNSTGSHIVADSKHEQFAAKTISAAFLKARRVSGLT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
WQGDPPSFHEIRSLAARLYTDEKGRDYAQKLLGHKSSEMTDKYRDVRGSEWAEIE
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ARRRSHKNRDLPPNLQPINGGYFCYRDPRTGKEYGLGRNRREAITQAIAANMAIYGSSK
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHEECCCC
QSPLVDRISGTQATTVSEWTTRYLTILEGRKLKPKTMTEHRKYLKAINEHLGEFAIQQVQ
CCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TKDVAAFLNLWVKAGKETMANKLRKEVIDLFREAIADGIVSHNPAEATRNQRVEIQRERI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHH
TLEEWKVIRKIAEKLPAWVGSCLDLALVTGQRLGDIQALLWENFKDGRLYIKQAKTGMML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCHHH
AFSAVTRIDCLNLCLADVLSQFKALNSTGSHIVADSKHEQFAAKTISAAFLKARRVSGLT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
WQGDPPSFHEIRSLAARLYTDEKGRDYAQKLLGHKSSEMTDKYRDVRGSEWAEIE
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]