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The map label for this gene is dacA [H]

Identifier: 85058773

GI number: 85058773

Start: 1347264

End: 1348475

Strand: Reverse

Name: dacA [H]

Synonym: SG0795

Alternate gene names: 85058773

Gene position: 1348475-1347264 (Counterclockwise)

Preceding gene: 85058774

Following gene: 85058772

Centisome position: 32.33

GC content: 54.46

Gene sequence:

>1212_bases
ATGAAAAATGTAACTACACCTCTCTTATTCAGTCGGTTAGCCTTAAGTACCGTTTTGGTGATGGGCGTTGTTTCCACAAG
CTTTGCCGATGATGTCAATCTGAAGACCATGATTCCGGGCGTACCGCAGATCGACGCCGAATCCTATATCCTCATCGATT
ACAATTCCGGTAAAGTGCTGACGGAAATGAACGCCGACGTGCGCCGCGATCCCGCCAGCCTGACCAAAATGATGACCAGT
TACGTCATCGGCCAAGCGATCAAATCCGGCAAAATCACCCCGAATGATGTGGTTACGGTCAACAAAGATGCCTGGGCCAC
CGGCAATCCGGTTTTCAAAGGCTCCTCGCTGATGTTTTTGAAGCCCGGCGACCGGGTACCCGTGTCACAGCTGAACCGCG
GTATCATTCTCCAATCGGGTAATGACGCCTGCGTGGCAATGGCGGACTACGTCGCCGGCAGCCAGGACGCGTTTGTGGGG
TTGATGAACAATTATGTCGGCGCCCTTGGGCTGCAAAACACCCATTTTGAAACCGTGCATGGCCTGGATGCCCCCGGACA
GTTCAGCTCCGCCCGTGACATGGCGATGATCGGTAAAGCGCTGATCCGCGACGTACCGGACGAGTACGCCACCTATAAAG
AAAAAGAATTCACCTTCAACAACATTCGCCAGATGAACCGTAACGGCCTGTTATGGGACAGCAATCTGAACGTCGACGGC
ATCAAAACCGGCCATACCGAATCTGCGGGCTATAATCTGGTGGCCTCGGCGACCGAAGGCCAGATGCGGCTGATTTCAGC
GGTGCTCGGCGGACATTCAATGAAAGGCCGGGAAAACGAAAGCCGCAAGCTGATGACCTGGGGTTTCCGCTTCTTTGAAA
CCGTCGCGCCGCTGAAAGCCGGCAAAGAGTTCGCCGCCGAGCCGGTGTGGTTTGGCGATACCGACAGCGTTAAACTGGGC
GTGGACAGCGATGCCTATCTGACCATCCCGCGCGGCCGGATGAAGGATTTGAAGGCCAGCTATGTGCTGGACAATCAGGA
ATTGCATGCGCCGCTGGCGAAAAATCAGCGGGTAGGCACCATCAATTTTCAGCTGGACGGCAAAACCATTGAACAGCGCC
CGCTGGTGGTGCTGAATGAAGTGAAAGAAGGCAACTTCTTCAGCCGCATCGTCGATCATGTCAAGCTGATGTTCCACCAT
TGGTTCGGTTAA

Upstream 100 bases:

>100_bases
TAGCCGCACGCGGTGCCGTTTTGCCTGATGCCGCCTTTTTTGACATCTGGTATAGTGTGGCACGATTTTAACTTACCCCT
ACGGATGCCGTTGTCCTGAC

Downstream 100 bases:

>100_bases
TCCCTTCATCCCTTGTCAGGGGCGAATCAGCACCCCTATAATACCCCTATTGTTTCTGCTCCCGCCGCGGCGGGAGCGCC
TGCCGTCTGTTGTTACTACG

Product: D-alanyl-D-alanine carboxypeptidase fraction A

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKNVTTPLLFSRLALSTVLVMGVVSTSFADDVNLKTMIPGVPQIDAESYILIDYNSGKVLTEMNADVRRDPASLTKMMTS
YVIGQAIKSGKITPNDVVTVNKDAWATGNPVFKGSSLMFLKPGDRVPVSQLNRGIILQSGNDACVAMADYVAGSQDAFVG
LMNNYVGALGLQNTHFETVHGLDAPGQFSSARDMAMIGKALIRDVPDEYATYKEKEFTFNNIRQMNRNGLLWDSNLNVDG
IKTGHTESAGYNLVASATEGQMRLISAVLGGHSMKGRENESRKLMTWGFRFFETVAPLKAGKEFAAEPVWFGDTDSVKLG
VDSDAYLTIPRGRMKDLKASYVLDNQELHAPLAKNQRVGTINFQLDGKTIEQRPLVVLNEVKEGNFFSRIVDHVKLMFHH
WFG

Sequences:

>Translated_403_residues
MKNVTTPLLFSRLALSTVLVMGVVSTSFADDVNLKTMIPGVPQIDAESYILIDYNSGKVLTEMNADVRRDPASLTKMMTS
YVIGQAIKSGKITPNDVVTVNKDAWATGNPVFKGSSLMFLKPGDRVPVSQLNRGIILQSGNDACVAMADYVAGSQDAFVG
LMNNYVGALGLQNTHFETVHGLDAPGQFSSARDMAMIGKALIRDVPDEYATYKEKEFTFNNIRQMNRNGLLWDSNLNVDG
IKTGHTESAGYNLVASATEGQMRLISAVLGGHSMKGRENESRKLMTWGFRFFETVAPLKAGKEFAAEPVWFGDTDSVKLG
VDSDAYLTIPRGRMKDLKASYVLDNQELHAPLAKNQRVGTINFQLDGKTIEQRPLVVLNEVKEGNFFSRIVDHVKLMFHH
WFG
>Mature_403_residues
MKNVTTPLLFSRLALSTVLVMGVVSTSFADDVNLKTMIPGVPQIDAESYILIDYNSGKVLTEMNADVRRDPASLTKMMTS
YVIGQAIKSGKITPNDVVTVNKDAWATGNPVFKGSSLMFLKPGDRVPVSQLNRGIILQSGNDACVAMADYVAGSQDAFVG
LMNNYVGALGLQNTHFETVHGLDAPGQFSSARDMAMIGKALIRDVPDEYATYKEKEFTFNNIRQMNRNGLLWDSNLNVDG
IKTGHTESAGYNLVASATEGQMRLISAVLGGHSMKGRENESRKLMTWGFRFFETVAPLKAGKEFAAEPVWFGDTDSVKLG
VDSDAYLTIPRGRMKDLKASYVLDNQELHAPLAKNQRVGTINFQLDGKTIEQRPLVVLNEVKEGNFFSRIVDHVKLMFHH
WFG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=403, Percent_Identity=78.6600496277916, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1787062, Length=395, Percent_Identity=62.2784810126582, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI87082022, Length=389, Percent_Identity=51.6709511568123, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI87082054, Length=260, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44371; Mature: 44371

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVTTPLLFSRLALSTVLVMGVVSTSFADDVNLKTMIPGVPQIDAESYILIDYNSGKVL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEEECCCEEE
TEMNADVRRDPASLTKMMTSYVIGQAIKSGKITPNDVVTVNKDAWATGNPVFKGSSLMFL
EECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEE
KPGDRVPVSQLNRGIILQSGNDACVAMADYVAGSQDAFVGLMNNYVGALGLQNTHFETVH
CCCCCCCHHHCCCCEEEECCCCCEEEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHCC
GLDAPGQFSSARDMAMIGKALIRDVPDEYATYKEKEFTFNNIRQMNRNGLLWDSNLNVDG
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHCCCCEEECCCCCCCC
IKTGHTESAGYNLVASATEGQMRLISAVLGGHSMKGRENESRKLMTWGFRFFETVAPLKA
CCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHC
GKEFAAEPVWFGDTDSVKLGVDSDAYLTIPRGRMKDLKASYVLDNQELHAPLAKNQRVGT
CHHHCCCCCEECCCCCEEEECCCCCEEEECCCHHHHCCHHEEECCHHHCCCHHCCCEEEE
INFQLDGKTIEQRPLVVLNEVKEGNFFSRIVDHVKLMFHHWFG
EEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNVTTPLLFSRLALSTVLVMGVVSTSFADDVNLKTMIPGVPQIDAESYILIDYNSGKVL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEEECCCEEE
TEMNADVRRDPASLTKMMTSYVIGQAIKSGKITPNDVVTVNKDAWATGNPVFKGSSLMFL
EECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEE
KPGDRVPVSQLNRGIILQSGNDACVAMADYVAGSQDAFVGLMNNYVGALGLQNTHFETVH
CCCCCCCHHHCCCCEEEECCCCCEEEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHCC
GLDAPGQFSSARDMAMIGKALIRDVPDEYATYKEKEFTFNNIRQMNRNGLLWDSNLNVDG
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHCCCCEEECCCCCCCC
IKTGHTESAGYNLVASATEGQMRLISAVLGGHSMKGRENESRKLMTWGFRFFETVAPLKA
CCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHC
GKEFAAEPVWFGDTDSVKLGVDSDAYLTIPRGRMKDLKASYVLDNQELHAPLAKNQRVGT
CHHHCCCCCEECCCCCEEEECCCCCEEEECCCHHHHCCHHEEECCHHHCCCHHCCCEEEE
INFQLDGKTIEQRPLVVLNEVKEGNFFSRIVDHVKLMFHHWFG
EEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]