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The map label for this gene is priC [H]

Identifier: 85058665

GI number: 85058665

Start: 1161662

End: 1162195

Strand: Reverse

Name: priC [H]

Synonym: SG0687

Alternate gene names: 85058665

Gene position: 1162195-1161662 (Counterclockwise)

Preceding gene: 85058673

Following gene: 85058663

Centisome position: 27.86

GC content: 60.86

Gene sequence:

>534_bases
GTGCACGCATCTCAACTGATTACCGCCCTTGGCGCCCGACTTAACGAATTAGCCGCACGCATCGACACTCAGGGACACCC
ACTGGTGCAGCAATCCCGTTTCGATCCCCTGTTGTTCAGCGCGCGCGGCAACCGGCTTCAGGACTATCTGTCCGAGAGCC
GCGCAACGCTCTCCCATTTGACCAGCGCGGTGGAACAGGGCCGCACCGAGCGCGTTGCCTGGCTGGCCCAGCGGCTCACC
GATCAAATGTTGGCACTCTCACGCGAGCTGGCCACGCAAAATTTGCGTCACGAGCACCCAGCCAGCACGCCGGCCGAAGA
TGTGTATGCCCGACTGGCCGAGCATCAGGATTATGAGCGGCGGTTGCTGGCGATGATCTGCGATCGCGATAGCTTGCGCA
CTGCTGCAAACGATCTCGCCCGCACTCGGCAGCTACAGCAGGAAATCGCCGCGCTGGAAGGACGTCTTATGCGTTGCCGG
CAGGCATTGACCCGTCTGGAATATCAAATAGAGTGCCGGGAACGGGGAGAATAA

Upstream 100 bases:

>100_bases
CGCAAGGCTGCGCCCCCTGCGTTAATGCTGTGCATCACTGAAAACGACGGGGTATTATCATAAAAGTCGACTCCGATGTC
ATTCATTACAGGTAAATGGC

Downstream 100 bases:

>100_bases
ACAAAATAATTCATCCGGTGGAACAAATGGATTACCTTTTCATCTTTAAGAGATGTAAATAATATCATCTATACTTCAGT
TTACAGCCTGAGTTGTGCAG

Product: primosomal replication protein N

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MHASQLITALGARLNELAARIDTQGHPLVQQSRFDPLLFSARGNRLQDYLSESRATLSHLTSAVEQGRTERVAWLAQRLT
DQMLALSRELATQNLRHEHPASTPAEDVYARLAEHQDYERRLLAMICDRDSLRTAANDLARTRQLQQEIAALEGRLMRCR
QALTRLEYQIECRERGE

Sequences:

>Translated_177_residues
MHASQLITALGARLNELAARIDTQGHPLVQQSRFDPLLFSARGNRLQDYLSESRATLSHLTSAVEQGRTERVAWLAQRLT
DQMLALSRELATQNLRHEHPASTPAEDVYARLAEHQDYERRLLAMICDRDSLRTAANDLARTRQLQQEIAALEGRLMRCR
QALTRLEYQIECRERGE
>Mature_177_residues
MHASQLITALGARLNELAARIDTQGHPLVQQSRFDPLLFSARGNRLQDYLSESRATLSHLTSAVEQGRTERVAWLAQRLT
DQMLALSRELATQNLRHEHPASTPAEDVYARLAEHQDYERRLLAMICDRDSLRTAANDLARTRQLQQEIAALEGRLMRCR
QALTRLEYQIECRERGE

Specific function: PriA recognizes a specific hairpin sequence on bacteriophage phi X174 ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase [H]

COG id: COG3923

COG function: function code L; Primosomal replication protein N''

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786673, Length=170, Percent_Identity=34.1176470588235, Blast_Score=83, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010890 [H]

Pfam domain/function: PF07445 priB_priC [H]

EC number: NA

Molecular weight: Translated: 20274; Mature: 20274

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHASQLITALGARLNELAARIDTQGHPLVQQSRFDPLLFSARGNRLQDYLSESRATLSHL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHH
TSAVEQGRTERVAWLAQRLTDQMLALSRELATQNLRHEHPASTPAEDVYARLAEHQDYER
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHH
RLLAMICDRDSLRTAANDLARTRQLQQEIAALEGRLMRCRQALTRLEYQIECRERGE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHASQLITALGARLNELAARIDTQGHPLVQQSRFDPLLFSARGNRLQDYLSESRATLSHL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHH
TSAVEQGRTERVAWLAQRLTDQMLALSRELATQNLRHEHPASTPAEDVYARLAEHQDYER
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHH
RLLAMICDRDSLRTAANDLARTRQLQQEIAALEGRLMRCRQALTRLEYQIECRERGE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1856227; 9278503 [H]