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The map label for this gene is acrA [H]

Identifier: 85058663

GI number: 85058663

Start: 1159373

End: 1160569

Strand: Reverse

Name: acrA [H]

Synonym: SG0685

Alternate gene names: 85058663

Gene position: 1160569-1159373 (Counterclockwise)

Preceding gene: 85058665

Following gene: 85058662

Centisome position: 27.82

GC content: 56.22

Gene sequence:

>1197_bases
ATGAACAAAAACAGAGGGCTATTGCCTCTGGCAGCCGTTCTTATGCTTTCCGGCAGTTTTATTCTCGCAGGCTGTGATAA
TAAAGAAGCCAACAAAGCAGGAAATCAGCAGCAGGCTCCCGAAGTGGGCGTTGTGACGCTAAAAAAACAAGCACTGAATG
TCACCACCGACTTACCGGGCCGCACAGCCGCTTTTCGTGTCGCGGAGGTCCGCCCGCAGGTCGATGGTATTATCCTGAAA
CGTAACTTTGTCGAAGGCAGCGATGTTACGGCAGGTGTGTCTCTCTATCAGATTGACCCTGCAACCTATCAGGCCGCCTA
TAACAGCGCCAAGGGCGATCTGGCACAGGCACAGGCGAATGCCGGCATTGCCCGGGTGACCGTCAACCGCTATAAAAAAC
TGCTCTCCACCAGCTATGTCAGCCGCCAGGACTATGATCAGGCGGTGGCCACCCTGGCGCAAAATGATGCCGCCGTGCAG
ACCGCTCAGGCCGCGCTGAAAACCGCCCGCATTAATCTGGCTTACACCCGCGTCACGTCTCCGATAAGCGGCCGCATCGG
CAAATCCGCCTTCACCGAAGGCGCGTTGATCACCAGCGCGCAAACAACGGCCATGGCGACCGTGCAGCAGTTGGACCCTA
TGTATGTCGACGTGACTCAATCAAGTAACGATTTTCTGCGTCTGCGCCAGGAGCTGGAAAACGGCACGCTGAAACAGCGT
GACGGCAAGTCGACAGTGCATCTGCTGATGGAAAACGGCGGCGAATATGCGCAGACAGGTACGCTGGAATTTTCCGACGT
CACGGTGGATGAAACCACGGGCTCCATTACGCTGCGCGCCATTTTCCCTAATCCGCAACACACGCTGTTGCCGGGTATGT
TTGTACGCGCCAGATTGGATCAGGGCGTCAATGAAAACGCGTTACTCGTACCTCAACAGGGCGTAACCCACACGCCCCGC
GGCGAGGCGAGCGCCATGGTGGTCGATAGCAATAATAAAGTCGCTATCCGCCAAGTCACGGCGCCGCAGGCGATTGGCGA
TAAATGGTTGGTCACCGCCGGGTTACAGGAAGGCGACAAAGTCATCGTCGCCGGTGTGCAGAAGGTGAAACCCTGCATGC
AGGTCACTCCCAAAGAGGTGACCAACCAGGAAAAACAACCGGAACAACCTCAGCCTGACACCGCTAGCAAGTCTTAA

Upstream 100 bases:

>100_bases
TGTATGTAACATAGCATAATGAAAAATGTAACGCAGCACTGTCTTTGGGGCTTGTGATCACTTGATCAATTTTTTCAAAT
CGGAATCTTGGGGTTTATCT

Downstream 100 bases:

>100_bases
CAGGAACGGGTAACCCTTATGTCTAAGTACTTTATCGATCGGCCCATATTCGCATGGGTGATTGCCATTATCATCATGCT
GGCGGGGGCACTGTCGATAC

Product: acriflavin efflux protein AcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNKNRGLLPLAAVLMLSGSFILAGCDNKEANKAGNQQQAPEVGVVTLKKQALNVTTDLPGRTAAFRVAEVRPQVDGIILK
RNFVEGSDVTAGVSLYQIDPATYQAAYNSAKGDLAQAQANAGIARVTVNRYKKLLSTSYVSRQDYDQAVATLAQNDAAVQ
TAQAALKTARINLAYTRVTSPISGRIGKSAFTEGALITSAQTTAMATVQQLDPMYVDVTQSSNDFLRLRQELENGTLKQR
DGKSTVHLLMENGGEYAQTGTLEFSDVTVDETTGSITLRAIFPNPQHTLLPGMFVRARLDQGVNENALLVPQQGVTHTPR
GEASAMVVDSNNKVAIRQVTAPQAIGDKWLVTAGLQEGDKVIVAGVQKVKPCMQVTPKEVTNQEKQPEQPQPDTASKS

Sequences:

>Translated_398_residues
MNKNRGLLPLAAVLMLSGSFILAGCDNKEANKAGNQQQAPEVGVVTLKKQALNVTTDLPGRTAAFRVAEVRPQVDGIILK
RNFVEGSDVTAGVSLYQIDPATYQAAYNSAKGDLAQAQANAGIARVTVNRYKKLLSTSYVSRQDYDQAVATLAQNDAAVQ
TAQAALKTARINLAYTRVTSPISGRIGKSAFTEGALITSAQTTAMATVQQLDPMYVDVTQSSNDFLRLRQELENGTLKQR
DGKSTVHLLMENGGEYAQTGTLEFSDVTVDETTGSITLRAIFPNPQHTLLPGMFVRARLDQGVNENALLVPQQGVTHTPR
GEASAMVVDSNNKVAIRQVTAPQAIGDKWLVTAGLQEGDKVIVAGVQKVKPCMQVTPKEVTNQEKQPEQPQPDTASKS
>Mature_398_residues
MNKNRGLLPLAAVLMLSGSFILAGCDNKEANKAGNQQQAPEVGVVTLKKQALNVTTDLPGRTAAFRVAEVRPQVDGIILK
RNFVEGSDVTAGVSLYQIDPATYQAAYNSAKGDLAQAQANAGIARVTVNRYKKLLSTSYVSRQDYDQAVATLAQNDAAVQ
TAQAALKTARINLAYTRVTSPISGRIGKSAFTEGALITSAQTTAMATVQQLDPMYVDVTQSSNDFLRLRQELENGTLKQR
DGKSTVHLLMENGGEYAQTGTLEFSDVTVDETTGSITLRAIFPNPQHTLLPGMFVRARLDQGVNENALLVPQQGVTHTPR
GEASAMVVDSNNKVAIRQVTAPQAIGDKWLVTAGLQEGDKVIVAGVQKVKPCMQVTPKEVTNQEKQPEQPQPDTASKS

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=396, Percent_Identity=68.6868686868687, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1789665, Length=375, Percent_Identity=60.5333333333333, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1789929, Length=374, Percent_Identity=54.2780748663102, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI87082036, Length=317, Percent_Identity=29.9684542586751, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI87081796, Length=185, Percent_Identity=29.1891891891892, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42679; Mature: 42679

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNRGLLPLAAVLMLSGSFILAGCDNKEANKAGNQQQAPEVGVVTLKKQALNVTTDLPG
CCCCCCCHHHHHHHHHCCCEEEEECCCCCHHCCCCCCCCCCCCEEEEEHHHEEEECCCCC
RTAAFRVAEVRPQVDGIILKRNFVEGSDVTAGVSLYQIDPATYQAAYNSAKGDLAQAQAN
CCCEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHCCCCCCHHHHHHC
AGIARVTVNRYKKLLSTSYVSRQDYDQAVATLAQNDAAVQTAQAALKTARINLAYTRVTS
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEECC
PISGRIGKSAFTEGALITSAQTTAMATVQQLDPMYVDVTQSSNDFLRLRQELENGTLKQR
CCCCCCCCHHHCCCEEEECCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCC
DGKSTVHLLMENGGEYAQTGTLEFSDVTVDETTGSITLRAIFPNPQHTLLPGMFVRARLD
CCCCEEEEEEECCCCEEEECCEEECCEEEECCCCCEEEEEECCCCCCHHCCHHHHHHHHH
QGVNENALLVPQQGVTHTPRGEASAMVVDSNNKVAIRQVTAPQAIGDKWLVTAGLQEGDK
CCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCHHCCCCEEEEECCCCCCE
VIVAGVQKVKPCMQVTPKEVTNQEKQPEQPQPDTASKS
EEEECHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKNRGLLPLAAVLMLSGSFILAGCDNKEANKAGNQQQAPEVGVVTLKKQALNVTTDLPG
CCCCCCCHHHHHHHHHCCCEEEEECCCCCHHCCCCCCCCCCCCEEEEEHHHEEEECCCCC
RTAAFRVAEVRPQVDGIILKRNFVEGSDVTAGVSLYQIDPATYQAAYNSAKGDLAQAQAN
CCCEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHCCCCCCHHHHHHC
AGIARVTVNRYKKLLSTSYVSRQDYDQAVATLAQNDAAVQTAQAALKTARINLAYTRVTS
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEECC
PISGRIGKSAFTEGALITSAQTTAMATVQQLDPMYVDVTQSSNDFLRLRQELENGTLKQR
CCCCCCCCHHHCCCEEEECCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCC
DGKSTVHLLMENGGEYAQTGTLEFSDVTVDETTGSITLRAIFPNPQHTLLPGMFVRARLD
CCCCEEEEEEECCCCEEEECCEEECCEEEECCCCCEEEEEECCCCCCHHCCHHHHHHHHH
QGVNENALLVPQQGVTHTPRGEASAMVVDSNNKVAIRQVTAPQAIGDKWLVTAGLQEGDK
CCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCHHCCCCEEEEECCCCCCE
VIVAGVQKVKPCMQVTPKEVTNQEKQPEQPQPDTASKS
EEEECHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]