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The map label for this gene is hemL

Identifier: 85058478

GI number: 85058478

Start: 867384

End: 868664

Strand: Reverse

Name: hemL

Synonym: SG0500

Alternate gene names: 85058478

Gene position: 868664-867384 (Counterclockwise)

Preceding gene: 85058480

Following gene: 85058477

Centisome position: 20.83

GC content: 63.78

Gene sequence:

>1281_bases
ATGAGTCATTCCGCAAGTTTGTACGCCGAGGCGCAGCAGCTGATCCCCGGCGGGGTCAATTCACCGGTGCGTGCATTCAA
CGGCGTTGGTGGCACGCCGCTGTTTATCGCCAGCGCCGACGGCGCCCGTTTGACCGACGCCGACGGCAATAGCTATATCG
ACTATGTTGGCTCGTGGGGCCCTATGGTGCTGGGCCATAATCACCCGGCGATTCGCCAGGCGGTGCTGGACGCCGTGCAG
CGCGGTCTGAGTTTTGGCGCTCCTACGGAAATCGAGGTGAAAATGGCGCGGCTGCTGACCGAGCTGGTGCCCTCTATCGA
GAGTGTGCGCATGGTCAACTCCGGCACCGAAGCCACCATGAGCACCATCCGGTTGGCGCGCGGCTATACCGGCCGCGACA
GGGTAATCAAATTTGAAGGCTGCTATCACGGCCACGCGGACGGACTGCTGGTCAAGGCCGGATCCGGGGCGCTCACTCTG
GGGCAACCCAGTTCTCCCGGCGTCCCGGCGGATTATGCCAAATATACCCTCACCTGCAGCTATAACGACCTGCAATCGGT
GCGCGAGGCGTTTACGCTGTACCCGCGCGAGATCGCCTGTGTCATCGTTGAACCGGTGGCGGGGAATATGAACTGCGTCC
CGCCGCTGCCGGAATTTTTGCCCGGCCTACGCGCGCTGTGCGACGAGTTCGGCGCACTGTTGATTATCGATGAGGTGATG
ACCGGTTTCCGTGTCGAGCTGGGCGGGGCCCAGGCTTATTATGATGTGAAACCAGATTTGACCTGCCTGGGCAAGATCAT
CGGCGGCGGCATGCCGGTTGGCGCCTTCGGCGGCCGCCGTGAGGTGATGGCGGCGCTGGCGCCGACGGGGCCGGTCTATC
AGGCAGGTACCCTGTCGGGCAATCCGGTCGCCATTGCGGCGGGCTACGCCTGCCTGCGCGAGGTCGCCCGGCCGGGCGTA
CATCAGCAGCTAACGCAGCAGACCGAACGTCTGGCGCAGGGGCTGCTGGCGGCAGCGGAAGCGGCGGGCGTGCCTCTGGT
GGTCAACCGCGTCGGCGGTATGTTCGGCCTGTTCTTTACCGAGTCGCCGACGGTGACCTGCTTCCAGGACGTCCAAGCGT
GTGACGTGGATCGTTTTAAACGGTTCTTCCATCTGATGCTGGCGGCGGGGGTTTATCTGGCGCCGTCGGCGTTTGAAGCC
GGCTTCATGTCCCTGGCGCACGGCGATGCCGAAATTCGCCACACGGTGGAGGCCGCGGCACAGAGTTTTGCCCAGCTGTG
A

Upstream 100 bases:

>100_bases
TGCTCTTTGATGTTAAATGCCAGGCGCGGTGATTTCACAATGGCCGCCAGTTGATTACACTACAGGGGTCAGGTTAATTT
TGCCATTCAGGAGTCGATTG

Downstream 100 bases:

>100_bases
ACTGTCTGTCGCCAGGCTTGTGCTTGCGCGGCTTTTCCCGACAGTGGCCCCTATGCCATGCAGCCCCGTCAGGCGCGGTT
TGCTCCCGGCCCCGCCTGAC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSHSASLYAEAQQLIPGGVNSPVRAFNGVGGTPLFIASADGARLTDADGNSYIDYVGSWGPMVLGHNHPAIRQAVLDAVQ
RGLSFGAPTEIEVKMARLLTELVPSIESVRMVNSGTEATMSTIRLARGYTGRDRVIKFEGCYHGHADGLLVKAGSGALTL
GQPSSPGVPADYAKYTLTCSYNDLQSVREAFTLYPREIACVIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
TGFRVELGGAQAYYDVKPDLTCLGKIIGGGMPVGAFGGRREVMAALAPTGPVYQAGTLSGNPVAIAAGYACLREVARPGV
HQQLTQQTERLAQGLLAAAEAAGVPLVVNRVGGMFGLFFTESPTVTCFQDVQACDVDRFKRFFHLMLAAGVYLAPSAFEA
GFMSLAHGDAEIRHTVEAAAQSFAQL

Sequences:

>Translated_426_residues
MSHSASLYAEAQQLIPGGVNSPVRAFNGVGGTPLFIASADGARLTDADGNSYIDYVGSWGPMVLGHNHPAIRQAVLDAVQ
RGLSFGAPTEIEVKMARLLTELVPSIESVRMVNSGTEATMSTIRLARGYTGRDRVIKFEGCYHGHADGLLVKAGSGALTL
GQPSSPGVPADYAKYTLTCSYNDLQSVREAFTLYPREIACVIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
TGFRVELGGAQAYYDVKPDLTCLGKIIGGGMPVGAFGGRREVMAALAPTGPVYQAGTLSGNPVAIAAGYACLREVARPGV
HQQLTQQTERLAQGLLAAAEAAGVPLVVNRVGGMFGLFFTESPTVTCFQDVQACDVDRFKRFFHLMLAAGVYLAPSAFEA
GFMSLAHGDAEIRHTVEAAAQSFAQL
>Mature_425_residues
SHSASLYAEAQQLIPGGVNSPVRAFNGVGGTPLFIASADGARLTDADGNSYIDYVGSWGPMVLGHNHPAIRQAVLDAVQR
GLSFGAPTEIEVKMARLLTELVPSIESVRMVNSGTEATMSTIRLARGYTGRDRVIKFEGCYHGHADGLLVKAGSGALTLG
QPSSPGVPADYAKYTLTCSYNDLQSVREAFTLYPREIACVIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVMT
GFRVELGGAQAYYDVKPDLTCLGKIIGGGMPVGAFGGRREVMAALAPTGPVYQAGTLSGNPVAIAAGYACLREVARPGVH
QQLTQQTERLAQGLLAAAEAAGVPLVVNRVGGMFGLFFTESPTVTCFQDVQACDVDRFKRFFHLMLAAGVYLAPSAFEAG
FMSLAHGDAEIRHTVEAAAQSFAQL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=329, Percent_Identity=27.355623100304, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI13994255, Length=333, Percent_Identity=27.9279279279279, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI284507298, Length=244, Percent_Identity=28.6885245901639, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=79.3427230046948, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=450, Percent_Identity=29.5555555555556, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1787560, Length=358, Percent_Identity=30.1675977653631, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI145693181, Length=303, Percent_Identity=31.3531353135314, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1788044, Length=313, Percent_Identity=33.5463258785943, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1789759, Length=308, Percent_Identity=31.4935064935065, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1786991, Length=381, Percent_Identity=28.3464566929134, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=296, Percent_Identity=26.3513513513513, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25144274, Length=139, Percent_Identity=34.5323741007194, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=346, Percent_Identity=28.9017341040462, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=354, Percent_Identity=24.8587570621469, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324386, Length=420, Percent_Identity=21.9047619047619, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28574759, Length=316, Percent_Identity=27.2151898734177, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI161085790, Length=316, Percent_Identity=27.2151898734177, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357415, Length=295, Percent_Identity=25.7627118644068, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_SODGM (Q2NVQ0)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454180.1
- ProteinModelPortal:   Q2NVQ0
- SMR:   Q2NVQ0
- STRING:   Q2NVQ0
- GeneID:   3868279
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0500
- NMPDR:   fig|343509.6.peg.1130
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- PhylomeDB:   Q2NVQ0
- ProtClustDB:   PRK00062
- BioCyc:   SGLO343509:SG0500-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 44962; Mature: 44831

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSASLYAEAQQLIPGGVNSPVRAFNGVGGTPLFIASADGARLTDADGNSYIDYVGSWG
CCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEECCCCEEECCCCCCEEHCCCCCC
PMVLGHNHPAIRQAVLDAVQRGLSFGAPTEIEVKMARLLTELVPSIESVRMVNSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHH
STIRLARGYTGRDRVIKFEGCYHGHADGLLVKAGSGALTLGQPSSPGVPADYAKYTLTCS
HHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCEEEEEEC
YNDLQSVREAFTLYPREIACVIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
HHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHH
TGFRVELGGAQAYYDVKPDLTCLGKIIGGGMPVGAFGGRREVMAALAPTGPVYQAGTLSG
CCEEEEECCCEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPVAIAAGYACLREVARPGVHQQLTQQTERLAQGLLAAAEAAGVPLVVNRVGGMFGLFFT
CCEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCEEEEEE
ESPTVTCFQDVQACDVDRFKRFFHLMLAAGVYLAPSAFEAGFMSLAHGDAEIRHTVEAAA
CCCCEEHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHH
QSFAQL
HHHHCC
>Mature Secondary Structure 
SHSASLYAEAQQLIPGGVNSPVRAFNGVGGTPLFIASADGARLTDADGNSYIDYVGSWG
CCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEECCCCEEECCCCCCEEHCCCCCC
PMVLGHNHPAIRQAVLDAVQRGLSFGAPTEIEVKMARLLTELVPSIESVRMVNSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHH
STIRLARGYTGRDRVIKFEGCYHGHADGLLVKAGSGALTLGQPSSPGVPADYAKYTLTCS
HHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCEEEEEEC
YNDLQSVREAFTLYPREIACVIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
HHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHH
TGFRVELGGAQAYYDVKPDLTCLGKIIGGGMPVGAFGGRREVMAALAPTGPVYQAGTLSG
CCEEEEECCCEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPVAIAAGYACLREVARPGVHQQLTQQTERLAQGLLAAAEAAGVPLVVNRVGGMFGLFFT
CCEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCEEEEEE
ESPTVTCFQDVQACDVDRFKRFFHLMLAAGVYLAPSAFEAGFMSLAHGDAEIRHTVEAAA
CCCCEEHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHH
QSFAQL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA