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The map label for this gene is pcnB

Identifier: 85058468

GI number: 85058468

Start: 847125

End: 848537

Strand: Reverse

Name: pcnB

Synonym: SG0490

Alternate gene names: 85058468

Gene position: 848537-847125 (Counterclockwise)

Preceding gene: 85058469

Following gene: 85058467

Centisome position: 20.34

GC content: 57.89

Gene sequence:

>1413_bases
TTGTTACTATTGAGGTGTACTATTTTTACCCGAGTAGCCAACTTCTGCCGTAAGATGCTCAATCGTGAAAGCGTGGAAAC
GGCACCCGAGGATATACGCCCTTTGACGATTATCCCGCGCGACCAGCACACCATCTCCCGTAAAGAGATCAGTGAAAACG
CATTAAAGGTCCTTTACCGCCTGAACAAAGCGGGATACGTAGCCTACCTGGTCGGCGGTGGCGTGCGCGATCTGCTGCTG
GGTAAAAAGCCCAAGGACTTCGACATCACCACCAGCGCGACCCCGGAGCAGATGCGTAAACTGTTCCGCAACTGCCGTCT
GGTCGGCCGCCGTTTCCGGCTGGCTCACGTCATGTTCGGGCCCGAAATTATCGAAGTCGCCACCTTCCGCGGCCATCATC
AGGTGCTGGAGCCGGAAGAAGGAGCGACGGGGGCAGCCCTGACCGGGCAAAACGGCATGCTGCTGCGCGACAATATTTTC
GGTTCCATTGAAGAAGACGCCCAGCGCCGTGACTTTTCCGTGAACAGTCTCTATTACAGCGTGGCCGATTTCACCCTGCG
CGACTATACCGGAGGGCTTAAAGATTTACAGGAAGGCACTATTCGCCTGATTGGCGATCCGGAAACCCGCTACCGGGAAG
ATCCGGTTCGCATGCTGCGCGCGGTGCGCTTTGCCGCCAAGCTGGACATGACCATCAGCCGCGAAACCGCCGAACCGATT
CCCCGTTTGGCGGCGTTGCTGCACGACATTCCGCCCGCGCGGCTGTTTGAAGAAGCTCTCAAGCTGCTACAGGCGGGCTA
TGGTGAAGCCACTTACCACCTGCTGTGCGAATACCAGCTGTTCCAGCCGCTGTTTCCGCTGATTAGCCGTAACTTTACCG
AGCACGGCGACAGCTATATGGAGCAGATGGTCATCCGCGTTCTGGCCAACACCGATCAGCGTTTGCGCAGCGATATGCGG
GTCAATCCGGCCTTCTTGTTCGCCGCCATGTTGTGGTATCCGCTGCTGGAGCATGCGCAAAAACTGACCCAGGAGAGCGG
CCTGTCCTATTTCGACGCGTTTTCTCTGGCGATGAACGACATCCTCGACAAACAATGTAGAACACTGGCCATCCCGAAAC
GATTAACCGCCCTGATGCGGGATATGTGGCAACTGCAGCTGCGGCTATCGCGCCGCCAGGGCAAGCGCGCCCATAAGCTG
ATGGAACATCCCAAGTTCCGCGCCTCCTATGATTTGCTGACGCTACGCGCCGAAGTCGAAAAGAACGCTGAATTACAGCG
ACTAAGCCGCTGGTGGGGTGAGTTTCAGGTCGCCGCGCCGCCGCGGCAAAAAAATATGCTTACCACCCTTGGCGATGAGG
GTCCGACTCGTTCCCGTCCGCGCCGTCGGGCGCCCAATCGCGACAGCGCCTGA

Upstream 100 bases:

>100_bases
GCCGTCCACGCCCGCCCCGTAAGGGACAAAACACCCACCCATTCTCAAACTTAGCGGGGTGAGCTATTATTAGCCGCGGT
TTTAGGTTGTTCCTGTTTAT

Downstream 100 bases:

>100_bases
TGGAGTGCGTCTGGCTGGCTCTTGGCAGCAATCTGGCGGAACCTCTACAGCAGGTAAGCGCCGCCCTCGTGGCGCTGGCG
AGCCTGCCGCAAACCCGGTT

Product: poly(A) polymerase I

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MLLLRCTIFTRVANFCRKMLNRESVETAPEDIRPLTIIPRDQHTISRKEISENALKVLYRLNKAGYVAYLVGGGVRDLLL
GKKPKDFDITTSATPEQMRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHQVLEPEEGATGAALTGQNGMLLRDNIF
GSIEEDAQRRDFSVNSLYYSVADFTLRDYTGGLKDLQEGTIRLIGDPETRYREDPVRMLRAVRFAAKLDMTISRETAEPI
PRLAALLHDIPPARLFEEALKLLQAGYGEATYHLLCEYQLFQPLFPLISRNFTEHGDSYMEQMVIRVLANTDQRLRSDMR
VNPAFLFAAMLWYPLLEHAQKLTQESGLSYFDAFSLAMNDILDKQCRTLAIPKRLTALMRDMWQLQLRLSRRQGKRAHKL
MEHPKFRASYDLLTLRAEVEKNAELQRLSRWWGEFQVAAPPRQKNMLTTLGDEGPTRSRPRRRAPNRDSA

Sequences:

>Translated_470_residues
MLLLRCTIFTRVANFCRKMLNRESVETAPEDIRPLTIIPRDQHTISRKEISENALKVLYRLNKAGYVAYLVGGGVRDLLL
GKKPKDFDITTSATPEQMRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHQVLEPEEGATGAALTGQNGMLLRDNIF
GSIEEDAQRRDFSVNSLYYSVADFTLRDYTGGLKDLQEGTIRLIGDPETRYREDPVRMLRAVRFAAKLDMTISRETAEPI
PRLAALLHDIPPARLFEEALKLLQAGYGEATYHLLCEYQLFQPLFPLISRNFTEHGDSYMEQMVIRVLANTDQRLRSDMR
VNPAFLFAAMLWYPLLEHAQKLTQESGLSYFDAFSLAMNDILDKQCRTLAIPKRLTALMRDMWQLQLRLSRRQGKRAHKL
MEHPKFRASYDLLTLRAEVEKNAELQRLSRWWGEFQVAAPPRQKNMLTTLGDEGPTRSRPRRRAPNRDSA
>Mature_470_residues
MLLLRCTIFTRVANFCRKMLNRESVETAPEDIRPLTIIPRDQHTISRKEISENALKVLYRLNKAGYVAYLVGGGVRDLLL
GKKPKDFDITTSATPEQMRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHQVLEPEEGATGAALTGQNGMLLRDNIF
GSIEEDAQRRDFSVNSLYYSVADFTLRDYTGGLKDLQEGTIRLIGDPETRYREDPVRMLRAVRFAAKLDMTISRETAEPI
PRLAALLHDIPPARLFEEALKLLQAGYGEATYHLLCEYQLFQPLFPLISRNFTEHGDSYMEQMVIRVLANTDQRLRSDMR
VNPAFLFAAMLWYPLLEHAQKLTQESGLSYFDAFSLAMNDILDKQCRTLAIPKRLTALMRDMWQLQLRLSRRQGKRAHKL
MEHPKFRASYDLLTLRAEVEKNAELQRLSRWWGEFQVAAPPRQKNMLTTLGDEGPTRSRPRRRAPNRDSA

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=213, Percent_Identity=30.9859154929577, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI87081691, Length=450, Percent_Identity=77.5555555555556, Blast_Score=720, Evalue=0.0,
Organism=Escherichia coli, GI1789436, Length=230, Percent_Identity=31.304347826087, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321016, Length=191, Percent_Identity=27.2251308900524, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 54199; Mature: 54199

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLRCTIFTRVANFCRKMLNRESVETAPEDIRPLTIIPRDQHTISRKEISENALKVLYR
CEEEEHHHHHHHHHHHHHHHCCCHHHCCHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHH
LNKAGYVAYLVGGGVRDLLLGKKPKDFDITTSATPEQMRKLFRNCRLVGRRFRLAHVMFG
HCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PEIIEVATFRGHHQVLEPEEGATGAALTGQNGMLLRDNIFGSIEEDAQRRDFSVNSLYYS
CHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEECCCCCCCHHHHHHCCCCHHHHHHH
VADFTLRDYTGGLKDLQEGTIRLIGDPETRYREDPVRMLRAVRFAAKLDMTISRETAEPI
HHHHHHHHHCCCHHHHCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHCCEECHHHHHHH
PRLAALLHDIPPARLFEEALKLLQAGYGEATYHLLCEYQLFQPLFPLISRNFTEHGDSYM
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
EQMVIRVLANTDQRLRSDMRVNPAFLFAAMLWYPLLEHAQKLTQESGLSYFDAFSLAMND
HHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ILDKQCRTLAIPKRLTALMRDMWQLQLRLSRRQGKRAHKLMEHPKFRASYDLLTLRAEVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHEEHHHHH
KNAELQRLSRWWGEFQVAAPPRQKNMLTTLGDEGPTRSRPRRRAPNRDSA
CCHHHHHHHHHHCCEEECCCCCHHCHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLLLRCTIFTRVANFCRKMLNRESVETAPEDIRPLTIIPRDQHTISRKEISENALKVLYR
CEEEEHHHHHHHHHHHHHHHCCCHHHCCHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHH
LNKAGYVAYLVGGGVRDLLLGKKPKDFDITTSATPEQMRKLFRNCRLVGRRFRLAHVMFG
HCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PEIIEVATFRGHHQVLEPEEGATGAALTGQNGMLLRDNIFGSIEEDAQRRDFSVNSLYYS
CHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEECCCCCCCHHHHHHCCCCHHHHHHH
VADFTLRDYTGGLKDLQEGTIRLIGDPETRYREDPVRMLRAVRFAAKLDMTISRETAEPI
HHHHHHHHHCCCHHHHCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHCCEECHHHHHHH
PRLAALLHDIPPARLFEEALKLLQAGYGEATYHLLCEYQLFQPLFPLISRNFTEHGDSYM
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
EQMVIRVLANTDQRLRSDMRVNPAFLFAAMLWYPLLEHAQKLTQESGLSYFDAFSLAMND
HHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ILDKQCRTLAIPKRLTALMRDMWQLQLRLSRRQGKRAHKLMEHPKFRASYDLLTLRAEVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHEEHHHHH
KNAELQRLSRWWGEFQVAAPPRQKNMLTTLGDEGPTRSRPRRRAPNRDSA
CCHHHHHHHHHHCCEEECCCCCHHCHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]