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The map label for this gene is panB

Identifier: 85058466

GI number: 85058466

Start: 845509

End: 846297

Strand: Reverse

Name: panB

Synonym: SG0488

Alternate gene names: 85058466

Gene position: 846297-845509 (Counterclockwise)

Preceding gene: 85058467

Following gene: 85058465

Centisome position: 20.29

GC content: 61.85

Gene sequence:

>789_bases
ATGACCACGATTTCTCATTTGCGCAAATGGAAACAGCAACAGCGCAAATTCACCTCCATTACCGCTTACGACGCCGCATT
TGCCCGCCTGTTCGCCGAACAGGGGATTAAGGTGATGCTGGTGGGCGATTCTTTGGGCATGACCATGCAGGGCCATGACT
CGACGCTGCCGGTCACCCTGGAGGATATGGTTTATCATACCCGCTGCGTGCGGCGCGGCGCGCCCTCGTGCCTGCTGCTG
GCCGATCTGCCATTCATGAGCTACGCGACGCCGGCACAGACCTTTGAAAATGCGGCAGCGCTGATGCGCGCGGGCGCCAA
TATGGTCAAGCTTGAGGGCGGCGCATGGCTAGCCGATACCGTTAACGGTCTGACCGCGCGGGCGGTACCCGTCTGCGGCC
ACCTTGGCCTAACCCCGCAATCTGTGAATATCTTCGGCGGCTATAAAATTCAAGGGCGCGATGCGGTCGGCGCAGACCAG
CTGCTGGCCGACGCACGGACGCTGGAACAGGCCGGCGCCCAGTTGCTGGTGCTGGAATGCGTACCGGTCGCGCTGGCGGA
ACGCGTAACGCATGAGCTGAGCATTCCGGTTATCGGCATTGGTGCCGGCGCCGTGACCGACGGCCAGATCCTGGTCATGC
AGGATGCGCTCGGCATCACCGGCGATAGCGCACCCTCCTTCGCCAAAGATTTTCTGGCGGCAGGCGGGGATATTGCCGCC
GCCGTACGCCGCTACGTGCAAGAGGTCGAAACCGGCCAGTTTCCCGCGGCCGAACATAGCTTTCAATGA

Upstream 100 bases:

>100_bases
TGTGCGATAAACGCTATACCCCCGGCAAAGCTTCCATTAAAATAGGCTGCCATTCTTTAAGCTGACAGCGGACGCTCCCG
CGGCACAGAGGTTACACACC

Downstream 100 bases:

>100_bases
TGAAGGAGACTCCCGTGCTGATTATCGAAACGCCGCCAACACTGCGTCAGACGGTTAAACAGTGGAGTCAGGAGCATAAG
CGTATCGCCCTGATCCCGAC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTTISHLRKWKQQQRKFTSITAYDAAFARLFAEQGIKVMLVGDSLGMTMQGHDSTLPVTLEDMVYHTRCVRRGAPSCLLL
ADLPFMSYATPAQTFENAAALMRAGANMVKLEGGAWLADTVNGLTARAVPVCGHLGLTPQSVNIFGGYKIQGRDAVGADQ
LLADARTLEQAGAQLLVLECVPVALAERVTHELSIPVIGIGAGAVTDGQILVMQDALGITGDSAPSFAKDFLAAGGDIAA
AVRRYVQEVETGQFPAAEHSFQ

Sequences:

>Translated_262_residues
MTTISHLRKWKQQQRKFTSITAYDAAFARLFAEQGIKVMLVGDSLGMTMQGHDSTLPVTLEDMVYHTRCVRRGAPSCLLL
ADLPFMSYATPAQTFENAAALMRAGANMVKLEGGAWLADTVNGLTARAVPVCGHLGLTPQSVNIFGGYKIQGRDAVGADQ
LLADARTLEQAGAQLLVLECVPVALAERVTHELSIPVIGIGAGAVTDGQILVMQDALGITGDSAPSFAKDFLAAGGDIAA
AVRRYVQEVETGQFPAAEHSFQ
>Mature_261_residues
TTISHLRKWKQQQRKFTSITAYDAAFARLFAEQGIKVMLVGDSLGMTMQGHDSTLPVTLEDMVYHTRCVRRGAPSCLLLA
DLPFMSYATPAQTFENAAALMRAGANMVKLEGGAWLADTVNGLTARAVPVCGHLGLTPQSVNIFGGYKIQGRDAVGADQL
LADARTLEQAGAQLLVLECVPVALAERVTHELSIPVIGIGAGAVTDGQILVMQDALGITGDSAPSFAKDFLAAGGDIAAA
VRRYVQEVETGQFPAAEHSFQ

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=261, Percent_Identity=72.4137931034483, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6319653, Length=252, Percent_Identity=36.1111111111111, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_SODGM (Q2NVR2)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454168.1
- ProteinModelPortal:   Q2NVR2
- SMR:   Q2NVR2
- STRING:   Q2NVR2
- GeneID:   3868702
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0488
- NMPDR:   fig|343509.6.peg.1094
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- PhylomeDB:   Q2NVR2
- BioCyc:   SGLO343509:SG0488-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 27818; Mature: 27687

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: ACT_SITE 179-179 BINDING 82-82 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTISHLRKWKQQQRKFTSITAYDAAFARLFAEQGIKVMLVGDSLGMTMQGHDSTLPVTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCCEEH
EDMVYHTRCVRRGAPSCLLLADLPFMSYATPAQTFENAAALMRAGANMVKLEGGAWLADT
HHHHHHHHHHHCCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHH
VNGLTARAVPVCGHLGLTPQSVNIFGGYKIQGRDAVGADQLLADARTLEQAGAQLLVLEC
HCCCHHHHHHHHCCCCCCCCCEEEEECEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEHH
VPVALAERVTHELSIPVIGIGAGAVTDGQILVMQDALGITGDSAPSFAKDFLAAGGDIAA
HHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHH
AVRRYVQEVETGQFPAAEHSFQ
HHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TTISHLRKWKQQQRKFTSITAYDAAFARLFAEQGIKVMLVGDSLGMTMQGHDSTLPVTL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCCEEH
EDMVYHTRCVRRGAPSCLLLADLPFMSYATPAQTFENAAALMRAGANMVKLEGGAWLADT
HHHHHHHHHHHCCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHH
VNGLTARAVPVCGHLGLTPQSVNIFGGYKIQGRDAVGADQLLADARTLEQAGAQLLVLEC
HCCCHHHHHHHHCCCCCCCCCEEEEECEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEHH
VPVALAERVTHELSIPVIGIGAGAVTDGQILVMQDALGITGDSAPSFAKDFLAAGGDIAA
HHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHH
AVRRYVQEVETGQFPAAEHSFQ
HHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA