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The map label for this gene is ptsN [H]

Identifier: 85058175

GI number: 85058175

Start: 377763

End: 378242

Strand: Reverse

Name: ptsN [H]

Synonym: SG0197

Alternate gene names: 85058175

Gene position: 378242-377763 (Counterclockwise)

Preceding gene: 85058176

Following gene: 85058174

Centisome position: 9.07

GC content: 55.42

Gene sequence:

>480_bases
ATGACTAACGATACAGCAATGCAAATCGATTCAGTGTTAAACATTGAATGTACCCGTAGCGACGTCCACTGCCAGAGCAA
GAAAAGAGCGCTGGAAATTATCAGCGAACTGGCGGCGAAACAACTTAACCTGGCGCCGCAAGTGGTGTTTGACGCCGTAT
TGACGCGCGAGCGCATGGGCAGTACCGGTATCGGCAACGGCATCGCCATTCCGCATGGTAAGCTGGAAGAAGACACGCTG
CGCGCGGTCGGGGTCTTTATCCAGCTCGAACAACCCATCGCGTTTGATGCCATCGATAATCAGCCAGTGGACCTGCTGTT
CGCGCTGCTGGTGCCGGCAGAGCAGTGCAAAGTCCATTTGCACACCCTTTCGCTTGTGGCCAAGCGGTTGGCGGATAAAA
CCGTCTGCCGCCGTTTGCGCGCCGCGCAAAGCGATGAGGAGCTGTATCAAATCATGACCGAAACGGAAGACGGCGACTGA

Upstream 100 bases:

>100_bases
CCGTAGCCGACAACGCTACCTAAACCTCACATCGGCTCGTTATCCCTACGGTAACGGGCCGATGAGTCTTAAAGGTTAAC
CAGCGCCTAAGTGACCCGAA

Downstream 100 bases:

>100_bases
CGCCGCGGCGCCGTCCTGAAAGCAGGATAGGTTGGGCGAAGCATGGCCGCATCGCCAGGGGATGAATAAGCAAGGCAGAG
GGGTGTATCGTCATGGTGCT

Product: PTS system transporter subunit IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; PTS system EIIA component; Phosphotransferase enzyme IIA component [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTNDTAMQIDSVLNIECTRSDVHCQSKKRALEIISELAAKQLNLAPQVVFDAVLTRERMGSTGIGNGIAIPHGKLEEDTL
RAVGVFIQLEQPIAFDAIDNQPVDLLFALLVPAEQCKVHLHTLSLVAKRLADKTVCRRLRAAQSDEELYQIMTETEDGD

Sequences:

>Translated_159_residues
MTNDTAMQIDSVLNIECTRSDVHCQSKKRALEIISELAAKQLNLAPQVVFDAVLTRERMGSTGIGNGIAIPHGKLEEDTL
RAVGVFIQLEQPIAFDAIDNQPVDLLFALLVPAEQCKVHLHTLSLVAKRLADKTVCRRLRAAQSDEELYQIMTETEDGD
>Mature_158_residues
TNDTAMQIDSVLNIECTRSDVHCQSKKRALEIISELAAKQLNLAPQVVFDAVLTRERMGSTGIGNGIAIPHGKLEEDTLR
AVGVFIQLEQPIAFDAIDNQPVDLLFALLVPAEQCKVHLHTLSLVAKRLADKTVCRRLRAAQSDEELYQIMTETEDGD

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=154, Percent_Identity=87.6623376623377, Blast_Score=282, Evalue=8e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17568; Mature: 17437

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDTAMQIDSVLNIECTRSDVHCQSKKRALEIISELAAKQLNLAPQVVFDAVLTRERMG
CCCCHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
STGIGNGIAIPHGKLEEDTLRAVGVFIQLEQPIAFDAIDNQPVDLLFALLVPAEQCKVHL
CCCCCCCEECCCCCCCHHHHHHHHHHEEECCCEEECCCCCCHHHHHHHHHCCHHHHHHHH
HTLSLVAKRLADKTVCRRLRAAQSDEELYQIMTETEDGD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TNDTAMQIDSVLNIECTRSDVHCQSKKRALEIISELAAKQLNLAPQVVFDAVLTRERMG
CCCHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
STGIGNGIAIPHGKLEEDTLRAVGVFIQLEQPIAFDAIDNQPVDLLFALLVPAEQCKVHL
CCCCCCCEECCCCCCCHHHHHHHHHHEEECCCEEECCCCCCHHHHHHHHHCCHHHHHHHH
HTLSLVAKRLADKTVCRRLRAAQSDEELYQIMTETEDGD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7876255; 8444818; 8025669; 9278503; 9636714 [H]