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The map label for this gene is aaeR [H]

Identifier: 85058140

GI number: 85058140

Start: 286774

End: 287706

Strand: Reverse

Name: aaeR [H]

Synonym: SG0162

Alternate gene names: 85058140

Gene position: 287706-286774 (Counterclockwise)

Preceding gene: 85058143

Following gene: 85058132

Centisome position: 6.9

GC content: 54.13

Gene sequence:

>933_bases
GTGAATAATGGAGAGACTAAAACGCATGCTAATCTTCGCCCGGTGGTGGAGGCCAATTCGTTTACCAGCGCCGCCCGCCG
ACTGCAAATGAGCATCTCATCCGTCAGTCAGACCGTGGCGAAACTGGAAAATGAATTGCAGGTTAAGCTGCTCAACCACA
GTACCCGCAGCATCGGTCTTACCGAAGCCGGCAAGATTTACTATCAGGGTTGTCGGCGGATGTTGCAGGAATTCAAAGAG
GTGTACGAGCAATTGTTCGCCTTTAACAATACTCCTATCGGTACTTTGCGCATCGGTAGCTCGTCGATGCTGGCCCATAA
TGTCCTGGCCTCGATAACCGCCACAATGCTGAAGGAGTATCCCGGCCTGTCGGTCAATCTGGTGACCGGCATTCCGGCGC
CGGACCTTATCGCCGACGGCCTGGATCTGGTGGTCCGGGTGGACACGCTGCAGGATTCCGGACTGTTTTCGCGCCGGGTG
GGAATTATGCCGATGGTGGTGTGCGCCGCCAAAAGCTATCTGGCACAGTGGTGCACGCCGGAAAAACCCGGCGATATAGC
CAATTTCTCTTGGCTGGAGTATGACGTGCGTCCCGACAGCGAGTTTGAGCTTATGACGCCGGAAGGGAGTACGCTGCATC
TGTCACCGGCCGATCGCTTTGTCACCAACGACTCCCAGACTCTGATTCGCTGGCTTATCGCCGGCGCAGGCATTTGTTAT
GTGCCAATCATGTGGGTTTTGGACGAGATAAGACGCGGCAATGTCGAGATATTATTCAGCCGTTATCACTCCGATCCACG
GCCTGTCTACGCAGTCTATACGACGCGTGATAAGTTGCCGCTCAAAGTACAAGTGTGCATTAACTATTTGACCGACTATT
TCAACAGGGTGACGGAAATGTACCAGACCTTCCGTCAGGACAGCGGAGGGTAA

Upstream 100 bases:

>100_bases
ACAGGCTGATAGGCGCAATACACTGAACCAACGCTGCTATATTATCCTGTACATTCCAGCGGTTTCAACATAACGTGATA
TAAATCCCTTTTAAGCCAGA

Downstream 100 bases:

>100_bases
AACCCGGCAAGCACGGTGGCGTGAGACGCTTGAGAGGCCTGCCGCGCAAGCTAAGTATCTTGCGGCGCTAGCGCAGCAGC
TCAGGCGGTACCACCCACGG

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNNGETKTHANLRPVVEANSFTSAARRLQMSISSVSQTVAKLENELQVKLLNHSTRSIGLTEAGKIYYQGCRRMLQEFKE
VYEQLFAFNNTPIGTLRIGSSSMLAHNVLASITATMLKEYPGLSVNLVTGIPAPDLIADGLDLVVRVDTLQDSGLFSRRV
GIMPMVVCAAKSYLAQWCTPEKPGDIANFSWLEYDVRPDSEFELMTPEGSTLHLSPADRFVTNDSQTLIRWLIAGAGICY
VPIMWVLDEIRRGNVEILFSRYHSDPRPVYAVYTTRDKLPLKVQVCINYLTDYFNRVTEMYQTFRQDSGG

Sequences:

>Translated_310_residues
MNNGETKTHANLRPVVEANSFTSAARRLQMSISSVSQTVAKLENELQVKLLNHSTRSIGLTEAGKIYYQGCRRMLQEFKE
VYEQLFAFNNTPIGTLRIGSSSMLAHNVLASITATMLKEYPGLSVNLVTGIPAPDLIADGLDLVVRVDTLQDSGLFSRRV
GIMPMVVCAAKSYLAQWCTPEKPGDIANFSWLEYDVRPDSEFELMTPEGSTLHLSPADRFVTNDSQTLIRWLIAGAGICY
VPIMWVLDEIRRGNVEILFSRYHSDPRPVYAVYTTRDKLPLKVQVCINYLTDYFNRVTEMYQTFRQDSGG
>Mature_310_residues
MNNGETKTHANLRPVVEANSFTSAARRLQMSISSVSQTVAKLENELQVKLLNHSTRSIGLTEAGKIYYQGCRRMLQEFKE
VYEQLFAFNNTPIGTLRIGSSSMLAHNVLASITATMLKEYPGLSVNLVTGIPAPDLIADGLDLVVRVDTLQDSGLFSRRV
GIMPMVVCAAKSYLAQWCTPEKPGDIANFSWLEYDVRPDSEFELMTPEGSTLHLSPADRFVTNDSQTLIRWLIAGAGICY
VPIMWVLDEIRRGNVEILFSRYHSDPRPVYAVYTTRDKLPLKVQVCINYLTDYFNRVTEMYQTFRQDSGG

Specific function: Activates transcription of the aaeXAB operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=73.9583333333333, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI145693193, Length=282, Percent_Identity=29.0780141843972, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787128, Length=266, Percent_Identity=26.6917293233083, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=27.4914089347079, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=27.3972602739726, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=23.859649122807, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787589, Length=264, Percent_Identity=26.1363636363636, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34835; Mature: 34835

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNGETKTHANLRPVVEANSFTSAARRLQMSISSVSQTVAKLENELQVKLLNHSTRSIGL
CCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCC
TEAGKIYYQGCRRMLQEFKEVYEQLFAFNNTPIGTLRIGSSSMLAHNVLASITATMLKEY
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHC
PGLSVNLVTGIPAPDLIADGLDLVVRVDTLQDSGLFSRRVGIMPMVVCAAKSYLAQWCTP
CCCEEEEEECCCCHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EKPGDIANFSWLEYDVRPDSEFELMTPEGSTLHLSPADRFVTNDSQTLIRWLIAGAGICY
CCCCCCCCCEEEEEECCCCCCEEEECCCCCEEEECCCHHHCCCCHHHHHHHHHHCCCHHH
VPIMWVLDEIRRGNVEILFSRYHSDPRPVYAVYTTRDKLPLKVQVCINYLTDYFNRVTEM
HHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
YQTFRQDSGG
HHHHHHCCCC
>Mature Secondary Structure
MNNGETKTHANLRPVVEANSFTSAARRLQMSISSVSQTVAKLENELQVKLLNHSTRSIGL
CCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCC
TEAGKIYYQGCRRMLQEFKEVYEQLFAFNNTPIGTLRIGSSSMLAHNVLASITATMLKEY
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHC
PGLSVNLVTGIPAPDLIADGLDLVVRVDTLQDSGLFSRRVGIMPMVVCAAKSYLAQWCTP
CCCEEEEEECCCCHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EKPGDIANFSWLEYDVRPDSEFELMTPEGSTLHLSPADRFVTNDSQTLIRWLIAGAGICY
CCCCCCCCCEEEEEECCCCCCEEEECCCCCEEEECCCHHHCCCCHHHHHHHHHHCCCHHH
VPIMWVLDEIRRGNVEILFSRYHSDPRPVYAVYTTRDKLPLKVQVCINYLTDYFNRVTEM
HHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
YQTFRQDSGG
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]