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The map label for this gene is pitA [H]

Identifier: 85058053

GI number: 85058053

Start: 118069

End: 119667

Strand: Reverse

Name: pitA [H]

Synonym: SG0075

Alternate gene names: 85058053

Gene position: 119667-118069 (Counterclockwise)

Preceding gene: 85058054

Following gene: 85058052

Centisome position: 2.87

GC content: 58.6

Gene sequence:

>1599_bases
ATGCTACATCTATTCACCTGCATGGAATTCCATACCGGTTTAATGTTGGTGCTCGCATTAATTTTTGTTCTGGTTTATGA
GGCCATCAATGGCTTTCATGATACGGCCAACGCCGTGGCCACCGTCATCTACACCCGCGCACTGCGCTCCCAACTGGCGG
TCGCCATGTCCGGGGTGTTCAATTTTCTCGGGGTTCTGATGGGCGGATTGAGCGTGGCCTACGCCATCGTTCATCTCTTG
CCGACCGACCTTTTGCTCAATGTCAGTTCAGCCCACGGTCTGGCGATGATGTTTTCCATGCTGCTGGCGGCGATTCTGTG
GAACCTGGGCACCTGGTATTTCGGCCTGCCGGCCTCCAGCTCGCACACCCTTATCGGCGCAATTATCGGTATCGGTCTGA
CCAACGCGTTGGTCAGCGACAGCTCGGTGGTCCAGGCGCTCAATATCCCCAAATTGATTGAAATCTTCCTGTCGCTAATT
ATTTCTCCGATAGTGGGTTTGGTGCTAGCGGGCGCGATGGTGTTTGTTCTGCACCGTTATTGGAGCAGCACCAAAAAGCG
CCGCCGCATCCATCTGACGCCGGCGGAGCGTGAGAAAAAGGACGGCAAGCGCAAACCGCCGTTCTGGACACGTATCGCCC
TGATTTTCTCCGCCGCCGGAGTGAGTTTTTCCCACGGCGCCAATGACGGGCAGAAGGGGATCGGGCTTATCATGCTGGTA
CTAATAGGCGTTGCGCCGGCGGGATTTGTGGTCAATATGAACGCCAGCGGCTACGACATCAGCCGCACCCGCGATGCGGT
GAATCATTTGCAGCAATATTATACTCAGCACCCCGGCGCGTTCACCCATGTCGTTGGCCCGGATGAGCAACCCCTACCGT
CGCCGGCCGCGGAAAATGCCGTCCCCGGCGCCGCGCCTGCCGTTGACCCTGCTGTCCCCCCTGCCGCCATCGGCGCGCCG
ATGGCCAATGGCCAGAGGCTGGATCAAGGGGATACCCCCCAGCCACAGGAGTTTCATTGCGACCTGTCCCGGGCGCTGTT
CGCCATCAATCGCGCCTCGCTGCTGTTGGGCAATCTGGATAGCTATGAAACGCTTAACGCCGATCAGCGCTCGCACGTAC
GGCGTCTGCTGATGTGCATTGCCGACACCGCCGACAAAGTGGCGAAGCTGCCGGAAACCCCTTCTGCGGATAAACGATTC
TTATCGAACCTGCGCCAGGATCTGCTGTATACGGTAGAGTACGCGCCGATGTGGATCATTATCGCCGTAGCGCTGGCGCT
TTCGCTGGGCACCATGGTGGGCTGGCGTCGGGTGGCCACGACCATCGGCGAGAAAATCGGTAAGAAAGGCATGACCTATG
CCCAGGGGCTGTCGGCGCAGATGACGGCGGCGGTGTCCATCGGCGTCGCCAGCTATACCGGTATGCCGGTTTCTACCACC
CACGTGCTGTCATCCGCGGTCACCGGCACCATGTTGGTGGATGGCGGCGGTGTGCAGGGCAAGACCGTGAAGAATATACT
TCTGGCCTGGGTACTGACGCTGCCGGTGGCGATTATTCTGGCCGGTAGTCTTTACTGGCTGGCGCTGAAATTGATTTAG

Upstream 100 bases:

>100_bases
CGGCAAGAATATCAAAAAAAGGTCATATTTTCCTTTTCCCTTGGCCCTCTTGTCGCAGATAATGTGGCCCGTTCATGTCC
AAACAATGGCGTAACTCTCT

Downstream 100 bases:

>100_bases
CCTCAGCCGCACGCAGCAAGACGGCCAGCCGGCCGTCTTTTTTTTACCTGTCGTTCAGTGCCAGATAGCCAGAGCGATCA
GGCTGACCACAATCAGACCA

Product: low-affinity inorganic phosphate transport protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MLHLFTCMEFHTGLMLVLALIFVLVYEAINGFHDTANAVATVIYTRALRSQLAVAMSGVFNFLGVLMGGLSVAYAIVHLL
PTDLLLNVSSAHGLAMMFSMLLAAILWNLGTWYFGLPASSSHTLIGAIIGIGLTNALVSDSSVVQALNIPKLIEIFLSLI
ISPIVGLVLAGAMVFVLHRYWSSTKKRRRIHLTPAEREKKDGKRKPPFWTRIALIFSAAGVSFSHGANDGQKGIGLIMLV
LIGVAPAGFVVNMNASGYDISRTRDAVNHLQQYYTQHPGAFTHVVGPDEQPLPSPAAENAVPGAAPAVDPAVPPAAIGAP
MANGQRLDQGDTPQPQEFHCDLSRALFAINRASLLLGNLDSYETLNADQRSHVRRLLMCIADTADKVAKLPETPSADKRF
LSNLRQDLLYTVEYAPMWIIIAVALALSLGTMVGWRRVATTIGEKIGKKGMTYAQGLSAQMTAAVSIGVASYTGMPVSTT
HVLSSAVTGTMLVDGGGVQGKTVKNILLAWVLTLPVAIILAGSLYWLALKLI

Sequences:

>Translated_532_residues
MLHLFTCMEFHTGLMLVLALIFVLVYEAINGFHDTANAVATVIYTRALRSQLAVAMSGVFNFLGVLMGGLSVAYAIVHLL
PTDLLLNVSSAHGLAMMFSMLLAAILWNLGTWYFGLPASSSHTLIGAIIGIGLTNALVSDSSVVQALNIPKLIEIFLSLI
ISPIVGLVLAGAMVFVLHRYWSSTKKRRRIHLTPAEREKKDGKRKPPFWTRIALIFSAAGVSFSHGANDGQKGIGLIMLV
LIGVAPAGFVVNMNASGYDISRTRDAVNHLQQYYTQHPGAFTHVVGPDEQPLPSPAAENAVPGAAPAVDPAVPPAAIGAP
MANGQRLDQGDTPQPQEFHCDLSRALFAINRASLLLGNLDSYETLNADQRSHVRRLLMCIADTADKVAKLPETPSADKRF
LSNLRQDLLYTVEYAPMWIIIAVALALSLGTMVGWRRVATTIGEKIGKKGMTYAQGLSAQMTAAVSIGVASYTGMPVSTT
HVLSSAVTGTMLVDGGGVQGKTVKNILLAWVLTLPVAIILAGSLYWLALKLI
>Mature_532_residues
MLHLFTCMEFHTGLMLVLALIFVLVYEAINGFHDTANAVATVIYTRALRSQLAVAMSGVFNFLGVLMGGLSVAYAIVHLL
PTDLLLNVSSAHGLAMMFSMLLAAILWNLGTWYFGLPASSSHTLIGAIIGIGLTNALVSDSSVVQALNIPKLIEIFLSLI
ISPIVGLVLAGAMVFVLHRYWSSTKKRRRIHLTPAEREKKDGKRKPPFWTRIALIFSAAGVSFSHGANDGQKGIGLIMLV
LIGVAPAGFVVNMNASGYDISRTRDAVNHLQQYYTQHPGAFTHVVGPDEQPLPSPAAENAVPGAAPAVDPAVPPAAIGAP
MANGQRLDQGDTPQPQEFHCDLSRALFAINRASLLLGNLDSYETLNADQRSHVRRLLMCIADTADKVAKLPETPSADKRF
LSNLRQDLLYTVEYAPMWIIIAVALALSLGTMVGWRRVATTIGEKIGKKGMTYAQGLSAQMTAAVSIGVASYTGMPVSTT
HVLSSAVTGTMLVDGGGVQGKTVKNILLAWVLTLPVAIILAGSLYWLALKLI

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=533, Percent_Identity=68.1050656660413, Blast_Score=715, Evalue=0.0,
Organism=Escherichia coli, GI1789360, Length=533, Percent_Identity=64.9155722326454, Blast_Score=661, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 56898; Mature: 56898

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLFTCMEFHTGLMLVLALIFVLVYEAINGFHDTANAVATVIYTRALRSQLAVAMSGVF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFLGVLMGGLSVAYAIVHLLPTDLLLNVSSAHGLAMMFSMLLAAILWNLGTWYFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCC
SHTLIGAIIGIGLTNALVSDSSVVQALNIPKLIEIFLSLIISPIVGLVLAGAMVFVLHRY
CCHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSSTKKRRRIHLTPAEREKKDGKRKPPFWTRIALIFSAAGVSFSHGANDGQKGIGLIMLV
HHHHHHCCEEECCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
LIGVAPAGFVVNMNASGYDISRTRDAVNHLQQYYTQHPGAFTHVVGPDEQPLPSPAAENA
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
VPGAAPAVDPAVPPAAIGAPMANGQRLDQGDTPQPQEFHCDLSRALFAINRASLLLGNLD
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SYETLNADQRSHVRRLLMCIADTADKVAKLPETPSADKRFLSNLRQDLLYTVEYAPMWII
CCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAVALALSLGTMVGWRRVATTIGEKIGKKGMTYAQGLSAQMTAAVSIGVASYTGMPVSTT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHH
HVLSSAVTGTMLVDGGGVQGKTVKNILLAWVLTLPVAIILAGSLYWLALKLI
HHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLHLFTCMEFHTGLMLVLALIFVLVYEAINGFHDTANAVATVIYTRALRSQLAVAMSGVF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFLGVLMGGLSVAYAIVHLLPTDLLLNVSSAHGLAMMFSMLLAAILWNLGTWYFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCC
SHTLIGAIIGIGLTNALVSDSSVVQALNIPKLIEIFLSLIISPIVGLVLAGAMVFVLHRY
CCHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSSTKKRRRIHLTPAEREKKDGKRKPPFWTRIALIFSAAGVSFSHGANDGQKGIGLIMLV
HHHHHHCCEEECCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
LIGVAPAGFVVNMNASGYDISRTRDAVNHLQQYYTQHPGAFTHVVGPDEQPLPSPAAENA
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
VPGAAPAVDPAVPPAAIGAPMANGQRLDQGDTPQPQEFHCDLSRALFAINRASLLLGNLD
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SYETLNADQRSHVRRLLMCIADTADKVAKLPETPSADKRFLSNLRQDLLYTVEYAPMWII
CCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAVALALSLGTMVGWRRVATTIGEKIGKKGMTYAQGLSAQMTAAVSIGVASYTGMPVSTT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHH
HVLSSAVTGTMLVDGGGVQGKTVKNILLAWVLTLPVAIILAGSLYWLALKLI
HHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]