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The map label for this gene is glyS [H]

Identifier: 84625799

GI number: 84625799

Start: 4671051

End: 4673288

Strand: Reverse

Name: glyS [H]

Synonym: XOO_4142

Alternate gene names: 84625799

Gene position: 4673288-4671051 (Counterclockwise)

Preceding gene: 84625800

Following gene: 84625798

Centisome position: 94.6

GC content: 64.92

Gene sequence:

>2238_bases
ATGAGCGAACAACTTCCCCTGCTGATCGAACTGGGCACCGAAGAGCTGCCGGTCAAGGCGCTGCCGGATCTGGCGCAGGC
GTTTTTCGATGGTGTGCTCAGCGGCCTGGAAAAGCGCGGCATTGCCGTCACCCGTGGCGCTGCCAAACCGCTGTCCACCC
CGCGTCGTCTGGCGGTGCTGCTGCCGGGCGTGGCCACCGAGCAGCCGGAGCAGCGCTCCGAAGTGCTGGGACCGTATCTC
AACATCGCCCTCGACGCCGAAGGCCAACCGACCAAGGCATTGGCGGGTTTCGCGGCCAAGGCCGGCATCGACTGGACCGC
GCTGGAGCGCACCAGCGACGCCAAGGGCGAGCGTTTCGTGCACCGCGCGGTGACCCCGGGCGCGCGTACCGCCGAGCTGT
TGCCGGAGATCCTGCGCGAGGCCATCGCCGCGATGCCGATTCCCAAGCCGATGCGCTGGGGTGGGCACGAATACGCCTTC
GCACGGCCGGTGCAGTGGCTGGTGCTGCTGTTCGGCAACGAGGTGATCCCGGCCGAATTGCTGGGCGTGCGTGGCGATCG
CATCACGCGCGGCCACCGCTTCATGCATGAGGGCGCGGTCTCGCTGGCGGTGCCGGGCGACTATGTCGAAGCCTTGCGTG
CGGCGCATGTACTGGTGGATCCCGATGCGCGCCGCACCCGCATCGTCGAAGAAGTGGAAGCGTCCGCCAAGCAGGCCGGC
GGCAGCGCGCGCATCAGCGACGACAACCTGGAGCAGGTGGTCAACCTGGTCGAATGGCCGTCGGCGGTGCTGTGCAGTTT
CGAACGCGATTTCCTGGCGGTGCCGCAGGAAGCGTTGATCGAAACCATGGAGATCAACCAGAAATTCTTCCCGGTGCTGG
ACGACGGCGGCACCTTGACCGAGCAGTTCATCGGCATCGCCAACATCCAATCCAAGGACGTGGCCGAAGTCGCCAAGGGC
TACGAGCGCGTCATCCGTCCGCGCTTTGCCGACGCCAAGTTCTTCTTCGACGAAGACCTCAAGCAGGGTCTGCAGGCGAT
GGGCGATGGTCTGGCCAGCGTCACCTATCAGGCCAAGCTGGGCACGGTGGCCGACAAGGTCGCACGCGTGGCAGCGCTGG
CGGAAGTCATCGCGCCGCAGGTGGGCGCCGACCCGCTGCAGGCACGCCGCGCTGCAGAGTTGGCCAAGAACGACCTGCAA
TCGCGCATGGTCAACGAATTCCCGGAATTGCAGGGCATTGCCGGCCGCCATTACGCCAAGGCAGCCGGTGAGCCCAGCGA
GATTGCGCTGGCCATCGACCAGGCCTACCAACCACGCTTTGCCGGCGACGACATCGCACTGTCGCCGCTGGGCAAGGTGC
TGGCGATTGCCGAACGCCTGGATACCTTGGCCGGTGGATTTGCGGCCGGGTTGAAGCCGACCGGTAACAAGGATCCGTTT
GCGCTGCGGCGCAATGCGTTGGGGTTGGCGCGGACGGTGATTGAGAGTGGGTTTGATCTGTCCCTAACTGAATTGCTCAA
GAAAGCAGTCGGTTTGGTTAATTCCGCTTTGGCTTGGTCGCAAGCTAGCAAACAACAGACAGCGGTTGAGGCTGCCAAGG
TGCAAGGTGTCCATGTCGCAGAGCGTCAGAGCGGCGGATTATGGGTGCCAAACGTAGAGGCAACTTCAGCAGAAATCCTC
GACTTCATCCTCGACCGCCTACGCGGCTATTACGCCGACAAGGGCGTACCGGCTACGCATTTCAACGCGGTCGCCGCATT
GTTCTCCGGCACGTCCGAAGCCGTGACGGCACCGACCGGCGTAGGAGCGCACCCGGGCGCGACCCACGGCTCCCTTTACG
ACTTCGACCGCCGCATCGATGCCATCGGCATCTTCGCCACGTTGCCCGAAGCCCAAGCGTTGGCCGCGGCCAACAAGCGC
ATCCGCAATATCCTGCGCAAGGTGGAAGGCGAGATTCCCGGCGACATCGACACTGCGCTGTTGCGCGAACCGGCCGAAGA
AGCCCTGGCCGAAGCGGTGGAAGCCGCCATCGGCGCTACCGGCGATGCCTTGCATCGCCACGACTACGTTGCCGTGCTCG
CACGCTTGGCGCGCCTGCGCCCACAAGTGGATGCGTTCTTCGACGGCGTGATGGTCAATGCCGAAGACCCGCAGCTGCGT
GCCAACCGCCTTGCCCTGCTGAAGAAGCTCGGCGACCGCCTCGGCAGCGTCGCCGCCATCGAGCACTTGTCCAGCTGA

Upstream 100 bases:

>100_bases
ATGCACCCAAACTCCACAGAGCTTCGCCCGTACCCTCATCCGCCCCTTCCGGGGCACCTTCTCCCGACGGGAGAAGGGAA
TACGCAAAGGTCCATGTCAT

Downstream 100 bases:

>100_bases
TGTCCAGTTCGACGGGCGCGCTGCACTGGCAGACGGCGCAGGCCGCGTTGGCACGTCGCGACCTGGGTGTGGCGGGGTCC
GCACTGCAGGCGTTGCTGGA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 745; Mature: 744

Protein sequence:

>745_residues
MSEQLPLLIELGTEELPVKALPDLAQAFFDGVLSGLEKRGIAVTRGAAKPLSTPRRLAVLLPGVATEQPEQRSEVLGPYL
NIALDAEGQPTKALAGFAAKAGIDWTALERTSDAKGERFVHRAVTPGARTAELLPEILREAIAAMPIPKPMRWGGHEYAF
ARPVQWLVLLFGNEVIPAELLGVRGDRITRGHRFMHEGAVSLAVPGDYVEALRAAHVLVDPDARRTRIVEEVEASAKQAG
GSARISDDNLEQVVNLVEWPSAVLCSFERDFLAVPQEALIETMEINQKFFPVLDDGGTLTEQFIGIANIQSKDVAEVAKG
YERVIRPRFADAKFFFDEDLKQGLQAMGDGLASVTYQAKLGTVADKVARVAALAEVIAPQVGADPLQARRAAELAKNDLQ
SRMVNEFPELQGIAGRHYAKAAGEPSEIALAIDQAYQPRFAGDDIALSPLGKVLAIAERLDTLAGGFAAGLKPTGNKDPF
ALRRNALGLARTVIESGFDLSLTELLKKAVGLVNSALAWSQASKQQTAVEAAKVQGVHVAERQSGGLWVPNVEATSAEIL
DFILDRLRGYYADKGVPATHFNAVAALFSGTSEAVTAPTGVGAHPGATHGSLYDFDRRIDAIGIFATLPEAQALAAANKR
IRNILRKVEGEIPGDIDTALLREPAEEALAEAVEAAIGATGDALHRHDYVAVLARLARLRPQVDAFFDGVMVNAEDPQLR
ANRLALLKKLGDRLGSVAAIEHLSS

Sequences:

>Translated_745_residues
MSEQLPLLIELGTEELPVKALPDLAQAFFDGVLSGLEKRGIAVTRGAAKPLSTPRRLAVLLPGVATEQPEQRSEVLGPYL
NIALDAEGQPTKALAGFAAKAGIDWTALERTSDAKGERFVHRAVTPGARTAELLPEILREAIAAMPIPKPMRWGGHEYAF
ARPVQWLVLLFGNEVIPAELLGVRGDRITRGHRFMHEGAVSLAVPGDYVEALRAAHVLVDPDARRTRIVEEVEASAKQAG
GSARISDDNLEQVVNLVEWPSAVLCSFERDFLAVPQEALIETMEINQKFFPVLDDGGTLTEQFIGIANIQSKDVAEVAKG
YERVIRPRFADAKFFFDEDLKQGLQAMGDGLASVTYQAKLGTVADKVARVAALAEVIAPQVGADPLQARRAAELAKNDLQ
SRMVNEFPELQGIAGRHYAKAAGEPSEIALAIDQAYQPRFAGDDIALSPLGKVLAIAERLDTLAGGFAAGLKPTGNKDPF
ALRRNALGLARTVIESGFDLSLTELLKKAVGLVNSALAWSQASKQQTAVEAAKVQGVHVAERQSGGLWVPNVEATSAEIL
DFILDRLRGYYADKGVPATHFNAVAALFSGTSEAVTAPTGVGAHPGATHGSLYDFDRRIDAIGIFATLPEAQALAAANKR
IRNILRKVEGEIPGDIDTALLREPAEEALAEAVEAAIGATGDALHRHDYVAVLARLARLRPQVDAFFDGVMVNAEDPQLR
ANRLALLKKLGDRLGSVAAIEHLSS
>Mature_744_residues
SEQLPLLIELGTEELPVKALPDLAQAFFDGVLSGLEKRGIAVTRGAAKPLSTPRRLAVLLPGVATEQPEQRSEVLGPYLN
IALDAEGQPTKALAGFAAKAGIDWTALERTSDAKGERFVHRAVTPGARTAELLPEILREAIAAMPIPKPMRWGGHEYAFA
RPVQWLVLLFGNEVIPAELLGVRGDRITRGHRFMHEGAVSLAVPGDYVEALRAAHVLVDPDARRTRIVEEVEASAKQAGG
SARISDDNLEQVVNLVEWPSAVLCSFERDFLAVPQEALIETMEINQKFFPVLDDGGTLTEQFIGIANIQSKDVAEVAKGY
ERVIRPRFADAKFFFDEDLKQGLQAMGDGLASVTYQAKLGTVADKVARVAALAEVIAPQVGADPLQARRAAELAKNDLQS
RMVNEFPELQGIAGRHYAKAAGEPSEIALAIDQAYQPRFAGDDIALSPLGKVLAIAERLDTLAGGFAAGLKPTGNKDPFA
LRRNALGLARTVIESGFDLSLTELLKKAVGLVNSALAWSQASKQQTAVEAAKVQGVHVAERQSGGLWVPNVEATSAEILD
FILDRLRGYYADKGVPATHFNAVAALFSGTSEAVTAPTGVGAHPGATHGSLYDFDRRIDAIGIFATLPEAQALAAANKRI
RNILRKVEGEIPGDIDTALLREPAEEALAEAVEAAIGATGDALHRHDYVAVLARLARLRPQVDAFFDGVMVNAEDPQLRA
NRLALLKKLGDRLGSVAAIEHLSS

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=745, Percent_Identity=40.8053691275168, Blast_Score=500, Evalue=1e-142,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 79791; Mature: 79660

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQLPLLIELGTEELPVKALPDLAQAFFDGVLSGLEKRGIAVTRGAAKPLSTPRRLAVL
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHEE
LPGVATEQPEQRSEVLGPYLNIALDAEGQPTKALAGFAAKAGIDWTALERTSDAKGERFV
ECCCCCCCCHHHHHHCCCHHEEEECCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCHHHHH
HRAVTPGARTAELLPEILREAIAAMPIPKPMRWGGHEYAFARPVQWLVLLFGNEVIPAEL
HHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
LGVRGDRITRGHRFMHEGAVSLAVPGDYVEALRAAHVLVDPDARRTRIVEEVEASAKQAG
HCCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCC
GSARISDDNLEQVVNLVEWPSAVLCSFERDFLAVPQEALIETMEINQKFFPVLDDGGTLT
CCCCCCCHHHHHHHHHHHCCHHHHHHCCHHHHHCCHHHHHHHHHHCCHHCCEECCCCHHH
EQFIGIANIQSKDVAEVAKGYERVIRPRFADAKFFFDEDLKQGLQAMGDGLASVTYQAKL
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHEEHHHHH
GTVADKVARVAALAEVIAPQVGADPLQARRAAELAKNDLQSRMVNEFPELQGIAGRHYAK
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHH
AAGEPSEIALAIDQAYQPRFAGDDIALSPLGKVLAIAERLDTLAGGFAAGLKPTGNKDPF
CCCCCHHEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
ALRRNALGLARTVIESGFDLSLTELLKKAVGLVNSALAWSQASKQQTAVEAAKVQGVHVA
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
ERQSGGLWVPNVEATSAEILDFILDRLRGYYADKGVPATHFNAVAALFSGTSEAVTAPTG
ECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCCC
VGAHPGATHGSLYDFDRRIDAIGIFATLPEAQALAAANKRIRNILRKVEGEIPGDIDTAL
CCCCCCCCCCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LREPAEEALAEAVEAAIGATGDALHRHDYVAVLARLARLRPQVDAFFDGVMVNAEDPQLR
HHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEECCCCCHHH
ANRLALLKKLGDRLGSVAAIEHLSS
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEQLPLLIELGTEELPVKALPDLAQAFFDGVLSGLEKRGIAVTRGAAKPLSTPRRLAVL
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHEE
LPGVATEQPEQRSEVLGPYLNIALDAEGQPTKALAGFAAKAGIDWTALERTSDAKGERFV
ECCCCCCCCHHHHHHCCCHHEEEECCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCHHHHH
HRAVTPGARTAELLPEILREAIAAMPIPKPMRWGGHEYAFARPVQWLVLLFGNEVIPAEL
HHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
LGVRGDRITRGHRFMHEGAVSLAVPGDYVEALRAAHVLVDPDARRTRIVEEVEASAKQAG
HCCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCC
GSARISDDNLEQVVNLVEWPSAVLCSFERDFLAVPQEALIETMEINQKFFPVLDDGGTLT
CCCCCCCHHHHHHHHHHHCCHHHHHHCCHHHHHCCHHHHHHHHHHCCHHCCEECCCCHHH
EQFIGIANIQSKDVAEVAKGYERVIRPRFADAKFFFDEDLKQGLQAMGDGLASVTYQAKL
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHEEHHHHH
GTVADKVARVAALAEVIAPQVGADPLQARRAAELAKNDLQSRMVNEFPELQGIAGRHYAK
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHH
AAGEPSEIALAIDQAYQPRFAGDDIALSPLGKVLAIAERLDTLAGGFAAGLKPTGNKDPF
CCCCCHHEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
ALRRNALGLARTVIESGFDLSLTELLKKAVGLVNSALAWSQASKQQTAVEAAKVQGVHVA
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
ERQSGGLWVPNVEATSAEILDFILDRLRGYYADKGVPATHFNAVAALFSGTSEAVTAPTG
ECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCCC
VGAHPGATHGSLYDFDRRIDAIGIFATLPEAQALAAANKRIRNILRKVEGEIPGDIDTAL
CCCCCCCCCCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LREPAEEALAEAVEAAIGATGDALHRHDYVAVLARLARLRPQVDAFFDGVMVNAEDPQLR
HHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEECCCCCHHH
ANRLALLKKLGDRLGSVAAIEHLSS
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA