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The map label for this gene is insH10 [H]

Identifier: 84625657

GI number: 84625657

Start: 4508731

End: 4509873

Strand: Reverse

Name: insH10 [H]

Synonym: XOO_4000

Alternate gene names: 84625657

Gene position: 4509873-4508731 (Counterclockwise)

Preceding gene: 84625658

Following gene: 84625654

Centisome position: 91.29

GC content: 60.72

Gene sequence:

>1143_bases
ATGCAACTGACCTTCGGCGACGCGGAGTACAACGGCAAGCGCAAGCGGACGCGGCGTGAGGTGTTCTTGGCCGAGATGGA
CCAGGTCGTGCCGTGGAAGGGCCTGCTGGCGCTGATCGAGCCGCACTATCCAAAGTCGGGGCAGCCGGGGCGACAGCCGT
ATCGGCTGGAAACGATGCTGCGCATCCACTTTTTGCAGCAGTGGTATGCGCTGAGCGATCCCTCGGCGGAAGAAGCGCTG
TACGACACGGTGTCGATGCGCCGTTTCGCCAAGATCGGCGGGCTGGACGAGGTGCCGGATGAAACGACGATTCTCCACTT
CCGGCATCTGCTGGAGCGGCATGATCTGGCGCGCAAGCTGTTCAGGGTCAACGCGCACCTGTCGCGTAAAGGGCAGAGTC
TGCGGGGCGGGACGATCGTGGATGCCACGATCATTGCGGCGCGCAGTTCGACCAAGAACAAGCAGGGCGAGCGCGATCCG
GACATGCACCAGACCAAGAAAGGGAATCAGTATGACTTCGGGATGAAGGCGCACATCGGGGTGGACGATGACTCCGGGCT
GGTGCACCATGTCGAATGCACGGCGGCCAACGTGGCCGATATCACGCAAGCGCACAAGCTGCTGCACGGCAAGGAAGACA
CGGTGTGCGGGGACAGCGGCTACACCGGGCTTGAGAAGCGCGAGGAGATGAAGCGCAAGCGCACGCTGCGCTACCTGATC
GCCGAGAAACCCTCGAAGCTGAAGCAGATCAAGAACAAACGCGAACTGAAGTTGGCCAAGCGGTGGGAGCACACCAAGGC
CAGCCTGCGGGCGAAGGTGGAACACCCGTTCCGGGTGATCAAGCGTCAGTTTGGTTACGTCAAGGTGCGCTATCGCAGCC
TGGTCAAGAACACCGCGCAGATGCTGACGCTGTTTGCGCTGTCGAATCTGTGGCTGAAGCGCAAAGAGTTAATGCCTGCT
GCGGGGAAGGTGTGCCTGTCACCCGGGAAATACACCGAAAACGCGCCGGAAACGGCAAAAAATCAGGGGTTTGAGCGCCC
TCAGCGCCGCAGATGTGGCGTGCTTCATTTTCCGGCCGCGTTGATCAGGCCATCCCTAACGCCGGAATTAAGCCGAGTTG
CGAAGCAACTTCGGATTGAATGA

Upstream 100 bases:

>100_bases
GCCGTGTACAGCGGCGGCTTGAACGGTGAATTGTTCGGGAAACTCTGAACACCTCCCCCTGATCCTGCGACAATCGCACA
GACGCAACGTGACGACGACG

Downstream 100 bases:

>100_bases
GTTGTTAGGCAGTGAGTGGCGCAAAGGTTGGATGGTGCAACCATGCTCCCCAGCACTGCCGCGTGGGCCGAGTTTGCCAC
AGCTTACGGGATGGAGCTTG

Product: ISXoo6 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MQLTFGDAEYNGKRKRTRREVFLAEMDQVVPWKGLLALIEPHYPKSGQPGRQPYRLETMLRIHFLQQWYALSDPSAEEAL
YDTVSMRRFAKIGGLDEVPDETTILHFRHLLERHDLARKLFRVNAHLSRKGQSLRGGTIVDATIIAARSSTKNKQGERDP
DMHQTKKGNQYDFGMKAHIGVDDDSGLVHHVECTAANVADITQAHKLLHGKEDTVCGDSGYTGLEKREEMKRKRTLRYLI
AEKPSKLKQIKNKRELKLAKRWEHTKASLRAKVEHPFRVIKRQFGYVKVRYRSLVKNTAQMLTLFALSNLWLKRKELMPA
AGKVCLSPGKYTENAPETAKNQGFERPQRRRCGVLHFPAALIRPSLTPELSRVAKQLRIE

Sequences:

>Translated_380_residues
MQLTFGDAEYNGKRKRTRREVFLAEMDQVVPWKGLLALIEPHYPKSGQPGRQPYRLETMLRIHFLQQWYALSDPSAEEAL
YDTVSMRRFAKIGGLDEVPDETTILHFRHLLERHDLARKLFRVNAHLSRKGQSLRGGTIVDATIIAARSSTKNKQGERDP
DMHQTKKGNQYDFGMKAHIGVDDDSGLVHHVECTAANVADITQAHKLLHGKEDTVCGDSGYTGLEKREEMKRKRTLRYLI
AEKPSKLKQIKNKRELKLAKRWEHTKASLRAKVEHPFRVIKRQFGYVKVRYRSLVKNTAQMLTLFALSNLWLKRKELMPA
AGKVCLSPGKYTENAPETAKNQGFERPQRRRCGVLHFPAALIRPSLTPELSRVAKQLRIE
>Mature_380_residues
MQLTFGDAEYNGKRKRTRREVFLAEMDQVVPWKGLLALIEPHYPKSGQPGRQPYRLETMLRIHFLQQWYALSDPSAEEAL
YDTVSMRRFAKIGGLDEVPDETTILHFRHLLERHDLARKLFRVNAHLSRKGQSLRGGTIVDATIIAARSSTKNKQGERDP
DMHQTKKGNQYDFGMKAHIGVDDDSGLVHHVECTAANVADITQAHKLLHGKEDTVCGDSGYTGLEKREEMKRKRTLRYLI
AEKPSKLKQIKNKRELKLAKRWEHTKASLRAKVEHPFRVIKRQFGYVKVRYRSLVKNTAQMLTLFALSNLWLKRKELMPA
AGKVCLSPGKYTENAPETAKNQGFERPQRRRCGVLHFPAALIRPSLTPELSRVAKQLRIE

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: COG3039

COG function: function code L; Transposase and inactivated derivatives, IS5 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787634, Length=312, Percent_Identity=56.0897435897436, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1788303, Length=312, Percent_Identity=55.4487179487179, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI1789921, Length=312, Percent_Identity=55.1282051282051, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1789612, Length=312, Percent_Identity=55.1282051282051, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1789355, Length=312, Percent_Identity=55.1282051282051, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1788518, Length=312, Percent_Identity=55.1282051282051, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1788342, Length=312, Percent_Identity=55.1282051282051, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1787592, Length=312, Percent_Identity=55.1282051282051, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1786877, Length=312, Percent_Identity=55.1282051282051, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1786764, Length=312, Percent_Identity=55.1282051282051, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1786453, Length=312, Percent_Identity=55.1282051282051, Blast_Score=352, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 43679; Mature: 43679

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTFGDAEYNGKRKRTRREVFLAEMDQVVPWKGLLALIEPHYPKSGQPGRQPYRLETML
CCEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
RIHFLQQWYALSDPSAEEALYDTVSMRRFAKIGGLDEVPDETTILHFRHLLERHDLARKL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FRVNAHLSRKGQSLRGGTIVDATIIAARSSTKNKQGERDPDMHQTKKGNQYDFGMKAHIG
HHHHHHHHHCCCCCCCCEEEHHHHEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEEC
VDDDSGLVHHVECTAANVADITQAHKLLHGKEDTVCGDSGYTGLEKREEMKRKRTLRYLI
CCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AEKPSKLKQIKNKRELKLAKRWEHTKASLRAKVEHPFRVIKRQFGYVKVRYRSLVKNTAQ
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MLTLFALSNLWLKRKELMPAAGKVCLSPGKYTENAPETAKNQGFERPQRRRCGVLHFPAA
HHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHCCCCCHHHHCCCEEECCHH
LIRPSLTPELSRVAKQLRIE
HHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQLTFGDAEYNGKRKRTRREVFLAEMDQVVPWKGLLALIEPHYPKSGQPGRQPYRLETML
CCEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
RIHFLQQWYALSDPSAEEALYDTVSMRRFAKIGGLDEVPDETTILHFRHLLERHDLARKL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FRVNAHLSRKGQSLRGGTIVDATIIAARSSTKNKQGERDPDMHQTKKGNQYDFGMKAHIG
HHHHHHHHHCCCCCCCCEEEHHHHEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEEC
VDDDSGLVHHVECTAANVADITQAHKLLHGKEDTVCGDSGYTGLEKREEMKRKRTLRYLI
CCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AEKPSKLKQIKNKRELKLAKRWEHTKASLRAKVEHPFRVIKRQFGYVKVRYRSLVKNTAQ
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MLTLFALSNLWLKRKELMPAAGKVCLSPGKYTENAPETAKNQGFERPQRRRCGVLHFPAA
HHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHCCCCCHHHHCCCEEECCHH
LIRPSLTPELSRVAKQLRIE
HHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]