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The map label for this gene is int [H]

Identifier: 84625654

GI number: 84625654

Start: 4505738

End: 4506613

Strand: Reverse

Name: int [H]

Synonym: XOO_3997

Alternate gene names: 84625654

Gene position: 4506613-4505738 (Counterclockwise)

Preceding gene: 84625657

Following gene: 84625652

Centisome position: 91.22

GC content: 65.07

Gene sequence:

>876_bases
ATGGATGTGGTCTGGGGGGTTTTCAGGGGCGACCAAATAGAGGTCGAGGCGTTTCTCACCGAACTGGCGACGCGGGGCCA
GGTGTCGGCCGGCACGCAGAATCAGGCGCTGGCGGCACTGCTGTTCCTGTATCGCGAGGTTCTGGGCGTGGAGTTGCCCT
GGATGGAGAATCTGGTGCGCGCCAAGCGGCCGCGGCGCATTCCCGTGGTGCTCTCGGCCGAGGAAGTCGCGCGTTTGCTG
ACGATGCTGGAGGGGTCTTGCCGGCTGATGGCGGGGCTGCTGTACGGCAGCGGGATGCGGCTGCTGGAATGCCTGCGCTT
GCGGATCAAGGACGTGGATGTGGTTCGTGGCGAAATTGTGGTGCGCGTCGGCAAAGGGGGCAAGGATCGGTGGGTGCCGC
TGCCGCACAGTCTGCGTGGGGAATTGATGCAGCAGCGCGAACGGGCGTTGCTGCTGCACGCCGCTGATCTCGCCGAGGGG
GCAGGGCGGGCGTTCCTGCCACACGCGCTGGCGCGCAAGTATCCCAATGCCGATGTCGAGCACGGCTGGCAGTATCTGTT
TCCCGCCGCACCTCGCTCTGTGGATCCGCGCAGTGGGCGCGTTGGTCGGCATCACGTATCGGAAAAAGTCCTGCAGCGTG
CGGTGCAGGCGGCACGGCAACAGGCTGGCCTTGCCAAGCCTGCAACCTGCCACACGTTGCGGCATTCATTCGCAACCCAT
TTGCTGGAGGCCGGCCACGATATCCGCACCGTGCAGGAACTGCTCGGCCACAAGGACGTGGCAACCACACAGATCTATAC
GCATGTGCTGGGACGCGGTGCATCGGTGGTGCGTAGTCCGCTGGACGGGCTCAGCCGTCTTGGTGGCGAGGCGTAA

Upstream 100 bases:

>100_bases
GGACGGCGTGTCCGCATGGGCGAAAACGGCCAGAAACCAGGACGTAGCGTAACGAACACTGGCAATTCCAGCACACCGAA
AACGCAGATGAAGCCTTGCA

Downstream 100 bases:

>100_bases
CGCGACGGCGGCGACGCAGTGATCGGATGCTTGCTACCACGCCAGTAAAATCGCGTGTTCTGATACACCAGAGCGTGCGA
TGAGGACGGCCAATCGCCGC

Product: site-specific recombinase IntIA

Products: NA

Alternate protein names: E2 protein [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MDVVWGVFRGDQIEVEAFLTELATRGQVSAGTQNQALAALLFLYREVLGVELPWMENLVRAKRPRRIPVVLSAEEVARLL
TMLEGSCRLMAGLLYGSGMRLLECLRLRIKDVDVVRGEIVVRVGKGGKDRWVPLPHSLRGELMQQRERALLLHAADLAEG
AGRAFLPHALARKYPNADVEHGWQYLFPAAPRSVDPRSGRVGRHHVSEKVLQRAVQAARQQAGLAKPATCHTLRHSFATH
LLEAGHDIRTVQELLGHKDVATTQIYTHVLGRGASVVRSPLDGLSRLGGEA

Sequences:

>Translated_291_residues
MDVVWGVFRGDQIEVEAFLTELATRGQVSAGTQNQALAALLFLYREVLGVELPWMENLVRAKRPRRIPVVLSAEEVARLL
TMLEGSCRLMAGLLYGSGMRLLECLRLRIKDVDVVRGEIVVRVGKGGKDRWVPLPHSLRGELMQQRERALLLHAADLAEG
AGRAFLPHALARKYPNADVEHGWQYLFPAAPRSVDPRSGRVGRHHVSEKVLQRAVQAARQQAGLAKPATCHTLRHSFATH
LLEAGHDIRTVQELLGHKDVATTQIYTHVLGRGASVVRSPLDGLSRLGGEA
>Mature_291_residues
MDVVWGVFRGDQIEVEAFLTELATRGQVSAGTQNQALAALLFLYREVLGVELPWMENLVRAKRPRRIPVVLSAEEVARLL
TMLEGSCRLMAGLLYGSGMRLLECLRLRIKDVDVVRGEIVVRVGKGGKDRWVPLPHSLRGELMQQRERALLLHAADLAEG
AGRAFLPHALARKYPNADVEHGWQYLFPAAPRSVDPRSGRVGRHHVSEKVLQRAVQAARQQAGLAKPATCHTLRHSFATH
LLEAGHDIRTVQELLGHKDVATTQIYTHVLGRGASVVRSPLDGLSRLGGEA

Specific function: Putative integrase believed to be involved in the insertion of antibiotic resistance genes into plasmids and transposons [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1789261, Length=235, Percent_Identity=36.1702127659575, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790244, Length=213, Percent_Identity=32.3943661971831, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR011946
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 32130; Mature: 32130

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVVWGVFRGDQIEVEAFLTELATRGQVSAGTQNQALAALLFLYREVLGVELPWMENLVR
CCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
AKRPRRIPVVLSAEEVARLLTMLEGSCRLMAGLLYGSGMRLLECLRLRIKDVDVVRGEIV
HCCCCCCEEEECHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECEEE
VRVGKGGKDRWVPLPHSLRGELMQQRERALLLHAADLAEGAGRAFLPHALARKYPNADVE
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHH
HGWQYLFPAAPRSVDPRSGRVGRHHVSEKVLQRAVQAARQQAGLAKPATCHTLRHSFATH
CCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LLEAGHDIRTVQELLGHKDVATTQIYTHVLGRGASVVRSPLDGLSRLGGEA
HHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDVVWGVFRGDQIEVEAFLTELATRGQVSAGTQNQALAALLFLYREVLGVELPWMENLVR
CCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
AKRPRRIPVVLSAEEVARLLTMLEGSCRLMAGLLYGSGMRLLECLRLRIKDVDVVRGEIV
HCCCCCCEEEECHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECEEE
VRVGKGGKDRWVPLPHSLRGELMQQRERALLLHAADLAEGAGRAFLPHALARKYPNADVE
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHH
HGWQYLFPAAPRSVDPRSGRVGRHHVSEKVLQRAVQAARQQAGLAKPATCHTLRHSFATH
CCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LLEAGHDIRTVQELLGHKDVATTQIYTHVLGRGASVVRSPLDGLSRLGGEA
HHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2821509; 2823258; 3054482; 8195081; 2188588 [H]