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The map label for this gene is benK [H]

Identifier: 84625647

GI number: 84625647

Start: 4497880

End: 4499187

Strand: Reverse

Name: benK [H]

Synonym: XOO_3990

Alternate gene names: 84625647

Gene position: 4499187-4497880 (Counterclockwise)

Preceding gene: 84625648

Following gene: 84625646

Centisome position: 91.07

GC content: 64.45

Gene sequence:

>1308_bases
ATGCTTCAGGACACTGCACCGTCTGGGCACTTCGTGCTGGAACGCATGACGCCGTTTCAGTGGCGGGCCGTCGCGGTCTG
CGTATTGCTGACGATGCTTGATGGTTTCGACGTGATGACCATGGCGTTCACTGCACCGCACGTGTCAGCGGACTGGCAGT
TGTCGGGCAAGCTGCTGGGCATGCTGTTCAGCGCCGGCCTGATCGGCATGGCGGTTGGCGCGCTGGCGCTGGCGCCGTTG
GCTGATCGTGTCGGTCGTCGTGCGCTGATACTGGTCTGCCTGGTGATCCTGACCGTCGCCATGAGCATCTCCGCATTGGC
CGGCAGTGCATGGCAGCTCGGCGCGCTGCGGCTCATGACCGGTGTGGGTATTGGCGGTATGTTGGCCTGCGTTGCGGTCA
CGGCCGGCGAGTTTTCCAGCCCCCGATGGCGCAACACCGCGATCGTGCTACAGGTCACCGGCTACCCGGTCGGTGCCACG
CTGGGTGGCGTGATTGCCGAACTGCTGATACGGCAGTGGTCGTGGCCCTCGGTGTTCGTGCTCGGGGCGGTGGCATCGTT
GTTAAGTGTGCCCTTGGTGCTTGCGTTCCTGCCGGAGTCGCTGGAGTTCCTGATCATCCGTCGGCCGCCCGATGCCGTCG
CCCGCTTCAACGCGGTATTGGCACGGATGGGGATGCCGCCGTACGCCGTATTGCCGCCACCGCCGCAGTTGGCCACGCAG
GCGCAAGGCTATTCGGCCATGTTTGACGCTCCCATGCGGCGGCACACGCTGTTGGTTGCGCTTGGCTTTTTCTCGCTGAT
GTTCGCGTTCTACTTCGTGATCAGCTGGACGCCCAAGCTGCTGGTGACCTCGGGCGCGTCTGCTGCGGAAGGCATCACAG
GCGGAGTGCTTTTGAATCTTGGAGGCATTGGCGGCGGCATCCTGTTCAGTGGGTTGGCATCTTGCGTGCGGCTGTCGCGG
CTGACGGTAGGATCCCTGTTGCTGATGGGCTTGGCGATGCTGGCGTTCGGGTTGTGCAGTACGCAGTTGAACTGGGCCTT
CGCCATGGCCTTGCTCACCGGCGCAGCCATGTTCGCTGCGGTGGGCGGCATGTACGCCATTGTTCCCATCGTCTTCACCG
CTGCGGTGCGCTCAACTGCGTTGGGCTGGGCGATCGGGGTGGGGCGCTTCGGTGCCATCTTGTCGCCGTTCTGCGCCGGT
GTATTGCTGGATGCGGGATGGTCGCCGGCGCGGTTGTTTGTCGTGTGTGGTGCGCCGCTGCTGGTCGCCGCCGCAGCGGT
TGTCGCGATGCGGCTACCGGAACGCTGA

Upstream 100 bases:

>100_bases
CCTGCTTCAGCCTGCGCCAGTGTGCTCTTGCACGCTGAGGTGTCGAAGAACAACGACGTCGAGTTACATTCATCATCTCC
TCCCACCACAGGACACGCCA

Downstream 100 bases:

>100_bases
GCCGATCAGCGGGCGCTACCGGGCGCCCGCTCGGCGGCGGTCGGTGGACCAAAGAAATCCGGCTTGCTGCCGATAGTTCG
GAGGGCCGCCGCGTCTGTGG

Product: MFS transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MLQDTAPSGHFVLERMTPFQWRAVAVCVLLTMLDGFDVMTMAFTAPHVSADWQLSGKLLGMLFSAGLIGMAVGALALAPL
ADRVGRRALILVCLVILTVAMSISALAGSAWQLGALRLMTGVGIGGMLACVAVTAGEFSSPRWRNTAIVLQVTGYPVGAT
LGGVIAELLIRQWSWPSVFVLGAVASLLSVPLVLAFLPESLEFLIIRRPPDAVARFNAVLARMGMPPYAVLPPPPQLATQ
AQGYSAMFDAPMRRHTLLVALGFFSLMFAFYFVISWTPKLLVTSGASAAEGITGGVLLNLGGIGGGILFSGLASCVRLSR
LTVGSLLLMGLAMLAFGLCSTQLNWAFAMALLTGAAMFAAVGGMYAIVPIVFTAAVRSTALGWAIGVGRFGAILSPFCAG
VLLDAGWSPARLFVVCGAPLLVAAAAVVAMRLPER

Sequences:

>Translated_435_residues
MLQDTAPSGHFVLERMTPFQWRAVAVCVLLTMLDGFDVMTMAFTAPHVSADWQLSGKLLGMLFSAGLIGMAVGALALAPL
ADRVGRRALILVCLVILTVAMSISALAGSAWQLGALRLMTGVGIGGMLACVAVTAGEFSSPRWRNTAIVLQVTGYPVGAT
LGGVIAELLIRQWSWPSVFVLGAVASLLSVPLVLAFLPESLEFLIIRRPPDAVARFNAVLARMGMPPYAVLPPPPQLATQ
AQGYSAMFDAPMRRHTLLVALGFFSLMFAFYFVISWTPKLLVTSGASAAEGITGGVLLNLGGIGGGILFSGLASCVRLSR
LTVGSLLLMGLAMLAFGLCSTQLNWAFAMALLTGAAMFAAVGGMYAIVPIVFTAAVRSTALGWAIGVGRFGAILSPFCAG
VLLDAGWSPARLFVVCGAPLLVAAAAVVAMRLPER
>Mature_435_residues
MLQDTAPSGHFVLERMTPFQWRAVAVCVLLTMLDGFDVMTMAFTAPHVSADWQLSGKLLGMLFSAGLIGMAVGALALAPL
ADRVGRRALILVCLVILTVAMSISALAGSAWQLGALRLMTGVGIGGMLACVAVTAGEFSSPRWRNTAIVLQVTGYPVGAT
LGGVIAELLIRQWSWPSVFVLGAVASLLSVPLVLAFLPESLEFLIIRRPPDAVARFNAVLARMGMPPYAVLPPPPQLATQ
AQGYSAMFDAPMRRHTLLVALGFFSLMFAFYFVISWTPKLLVTSGASAAEGITGGVLLNLGGIGGGILFSGLASCVRLSR
LTVGSLLLMGLAMLAFGLCSTQLNWAFAMALLTGAAMFAAVGGMYAIVPIVFTAAVRSTALGWAIGVGRFGAILSPFCAG
VLLDAGWSPARLFVVCGAPLLVAAAAVVAMRLPER

Specific function: Probable uptake of benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=413, Percent_Identity=25.181598062954, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI87081723, Length=417, Percent_Identity=29.4964028776978, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788074, Length=350, Percent_Identity=24, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082159, Length=385, Percent_Identity=22.5974025974026, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082231, Length=163, Percent_Identity=28.2208588957055, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082404, Length=191, Percent_Identity=24.0837696335079, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788068, Length=424, Percent_Identity=21.9339622641509, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1786229, Length=381, Percent_Identity=23.0971128608924, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45374; Mature: 45374

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQDTAPSGHFVLERMTPFQWRAVAVCVLLTMLDGFDVMTMAFTAPHVSADWQLSGKLLG
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCCCCHHHHHHH
MLFSAGLIGMAVGALALAPLADRVGRRALILVCLVILTVAMSISALAGSAWQLGALRLMT
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GVGIGGMLACVAVTAGEFSSPRWRNTAIVLQVTGYPVGATLGGVIAELLIRQWSWPSVFV
HCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHH
LGAVASLLSVPLVLAFLPESLEFLIIRRPPDAVARFNAVLARMGMPPYAVLPPPPQLATQ
HHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEECCCCCHHHHHH
AQGYSAMFDAPMRRHTLLVALGFFSLMFAFYFVISWTPKLLVTSGASAAEGITGGVLLNL
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCHHCCCCCCEEEEE
GGIGGGILFSGLASCVRLSRLTVGSLLLMGLAMLAFGLCSTQLNWAFAMALLTGAAMFAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGGMYAIVPIVFTAAVRSTALGWAIGVGRFGAILSPFCAGVLLDAGWSPARLFVVCGAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHH
LVAAAAVVAMRLPER
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MLQDTAPSGHFVLERMTPFQWRAVAVCVLLTMLDGFDVMTMAFTAPHVSADWQLSGKLLG
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCCCCHHHHHHH
MLFSAGLIGMAVGALALAPLADRVGRRALILVCLVILTVAMSISALAGSAWQLGALRLMT
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GVGIGGMLACVAVTAGEFSSPRWRNTAIVLQVTGYPVGATLGGVIAELLIRQWSWPSVFV
HCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHH
LGAVASLLSVPLVLAFLPESLEFLIIRRPPDAVARFNAVLARMGMPPYAVLPPPPQLATQ
HHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEECCCCCHHHHHH
AQGYSAMFDAPMRRHTLLVALGFFSLMFAFYFVISWTPKLLVTSGASAAEGITGGVLLNL
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCHHCCCCCCEEEEE
GGIGGGILFSGLASCVRLSRLTVGSLLLMGLAMLAFGLCSTQLNWAFAMALLTGAAMFAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGGMYAIVPIVFTAAVRSTALGWAIGVGRFGAILSPFCAGVLLDAGWSPARLFVVCGAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHH
LVAAAAVVAMRLPER
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9294456 [H]