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The map label for this gene is lolB [H]

Identifier: 84625064

GI number: 84625064

Start: 3867631

End: 3868284

Strand: Reverse

Name: lolB [H]

Synonym: XOO_3407

Alternate gene names: 84625064

Gene position: 3868284-3867631 (Counterclockwise)

Preceding gene: 84625065

Following gene: 84625063

Centisome position: 78.3

GC content: 69.57

Gene sequence:

>654_bases
ATGAGCAAGGCATTGCGGACGCTGGCCCTGAGCGGGCTGGTGCTCGTCGGGCTATCGGCGTGCGTCAGCGTGCCGCGCGG
GCAGGGCAGTGGCGCGGCGGTAGTCGAGCAAATCTCGGACAGCGCCCGCCAGGGCGAAGCCGCCCGCCAAGCCTGGCTGC
AGCAGCATCCCAACTGGTCGTTCCAGGGCCGGGTGGCGATCAGCAAGGACCGCAATGGCGGCAGCGGTCGCATCGACTGG
CAGCAGGACGGCCCGCGCTACCGCGTGCAACTCAGTGCACCGGTCACCCGGCAAAGCTGGGTGCTCACTGGCGACACCAC
CACCGGAGCCGGCCGGCTGGAAGGACTGGACGGTGGCCCGCGTAGTGGCTCCGATGCCGAGAAGGTGTTGCTGGAAGCGA
CCGGCTGGACCATCCCGGTCAATCAGATGCCGGACTGGGTGCGCGCGCTGCGCATCGCCGACGCAGGTGCCGCCCGCGTT
GAGCTGGATGCCGCCGGGCGCCCGCGCACCGTACAGCAGGACGGCTGGACCATCGATTTTTTGGCCTGGACGCCGGCCAG
CGCCGACCAGCCCGAGCTTCCGCAGCGGATCGAAGCCCGTTACGGTGAGGCCAAGGTCCGTCTGCTGGTCGATCAGTGGA
CCGTATCGCCCTGA

Upstream 100 bases:

>100_bases
TTAGTGGCAAGCAGGACGAGGCACGCCGTTACTTCGATGAAGCGCGCCGCCTCGACCCGGACAACCGTGCGCTGCAACGC
GCAGTGGAAAAGTTCGGGCT

Downstream 100 bases:

>100_bases
TGAACGCGCCGGGCACTGATTGGTCGGCGTGGCCTGCGCCGGCCAAGCTCAATCTGTTCCTGCAGATCACCGGGCGCCGC
GCCGATGGCTATCACCTGCT

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSKALRTLALSGLVLVGLSACVSVPRGQGSGAAVVEQISDSARQGEAARQAWLQQHPNWSFQGRVAISKDRNGGSGRIDW
QQDGPRYRVQLSAPVTRQSWVLTGDTTTGAGRLEGLDGGPRSGSDAEKVLLEATGWTIPVNQMPDWVRALRIADAGAARV
ELDAAGRPRTVQQDGWTIDFLAWTPASADQPELPQRIEARYGEAKVRLLVDQWTVSP

Sequences:

>Translated_217_residues
MSKALRTLALSGLVLVGLSACVSVPRGQGSGAAVVEQISDSARQGEAARQAWLQQHPNWSFQGRVAISKDRNGGSGRIDW
QQDGPRYRVQLSAPVTRQSWVLTGDTTTGAGRLEGLDGGPRSGSDAEKVLLEATGWTIPVNQMPDWVRALRIADAGAARV
ELDAAGRPRTVQQDGWTIDFLAWTPASADQPELPQRIEARYGEAKVRLLVDQWTVSP
>Mature_216_residues
SKALRTLALSGLVLVGLSACVSVPRGQGSGAAVVEQISDSARQGEAARQAWLQQHPNWSFQGRVAISKDRNGGSGRIDWQ
QDGPRYRVQLSAPVTRQSWVLTGDTTTGAGRLEGLDGGPRSGSDAEKVLLEATGWTIPVNQMPDWVRALRIADAGAARVE
LDAAGRPRTVQQDGWTIDFLAWTPASADQPELPQRIEARYGEAKVRLLVDQWTVSP

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein [H]

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004565 [H]

Pfam domain/function: PF03550 LolB [H]

EC number: NA

Molecular weight: Translated: 23406; Mature: 23275

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKALRTLALSGLVLVGLSACVSVPRGQGSGAAVVEQISDSARQGEAARQAWLQQHPNWS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
FQGRVAISKDRNGGSGRIDWQQDGPRYRVQLSAPVTRQSWVLTGDTTTGAGRLEGLDGGP
EEEEEEEEECCCCCCCEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCCEECCCCCCC
RSGSDAEKVLLEATGWTIPVNQMPDWVRALRIADAGAARVELDAAGRPRTVQQDGWTIDF
CCCCHHHEEEEEECCCEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCCEECCCCCEEEE
LAWTPASADQPELPQRIEARYGEAKVRLLVDQWTVSP
EEECCCCCCCCCCHHHHHHHCCCEEEEEEEEEECCCC
>Mature Secondary Structure 
SKALRTLALSGLVLVGLSACVSVPRGQGSGAAVVEQISDSARQGEAARQAWLQQHPNWS
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
FQGRVAISKDRNGGSGRIDWQQDGPRYRVQLSAPVTRQSWVLTGDTTTGAGRLEGLDGGP
EEEEEEEEECCCCCCCEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCCEECCCCCCC
RSGSDAEKVLLEATGWTIPVNQMPDWVRALRIADAGAARVELDAAGRPRTVQQDGWTIDF
CCCCHHHEEEEEECCCEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCCEECCCCCEEEE
LAWTPASADQPELPQRIEARYGEAKVRLLVDQWTVSP
EEECCCCCCCCCCHHHHHHHCCCEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]