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The map label for this gene is cysB [H]

Identifier: 84624864

GI number: 84624864

Start: 3636495

End: 3637478

Strand: Reverse

Name: cysB [H]

Synonym: XOO_3207

Alternate gene names: 84624864

Gene position: 3637478-3636495 (Counterclockwise)

Preceding gene: 84624869

Following gene: 84624863

Centisome position: 73.63

GC content: 66.67

Gene sequence:

>984_bases
ATGACGCTGACCCAACTTCGCTATCTGGTGGCCATCGCCGATGCCGAGCTGAACATCACGCTGGCCGCTGGACGCGTGCA
CGCGACCCAGCCCGGCCTGTCCAAGCAGCTCAAGCAGCTGGAGGACGAGCTGGGCTTTCTGTTGTTCGTGCGCAAGGGGC
GCAGCCTGGATGGGGCAACGCCAGCCGGTGTGGAAGTGATCGAGCGTGCGCGTGCGGTGCTGTCGGAGGTCAACAATATC
CGCACCTATGCGGCCAACCAGCGCCGCGAAAGCCAGGGCCAGCTGACCCTGACCACCACCCACACCCAGGCGCGCTTCGT
GCTGCCGCCGGCAGTGGCGCAGATCAAGAACGCCTACCCGCAGGTCAGCGTGCATTTGCAACAGGCTGCCGAAAGTGCGG
CGCTGGATCTGCTCGGCCAGGGCGATGCGGATATTGCCGTGGTCAGCACTGCCGGCGGCGAGCCGAGTGCCGGGATTGCA
GTGCCGCTGTACCGCTGGCGGCGGCTGGTGGTGGTGCCGCGCGGGCATGCGTTGGATCACCCGCGCACGGCGCCGGAAAT
GCACGCGTTGTCCGAGCATCCGCTGATCAGCTACGACTCTTCCACGCGTCCGGGCTCATCGCTGCAGCGCGCGTTTGCGC
AGATGGGTCTGGAGCCGAGCATCGCGTTGACCGCGCTGGACGCGGATCTCATCAAAACGTACGTACGTGCCGGGCTCGGC
GTGGGCCTGGTGGCGGAGATGGCGGTCAATGCCTTCGACAAAGATCTACGCGCCTGGCCGGCGCCCGCACCGATTGCAGA
GTGCATCGCCTGGGCGGTGCTGCCACGCGACCGCGTGCTGCGCGACTACGCATTGGACCTGGTGCATGTGCTCGCGCCGC
AAATCGACAAGCGCGACCTGCGCCGCGTACTGGACGGCAATCAGGCGCCGAACTGGCCATTGCCGCCGACCTGGGAATCG
CTGACCCAGACCATCACCAGCTGA

Upstream 100 bases:

>100_bases
CATACACCCTAAAAATAGCGTTCGGTTATAAGCTTGAACGGAGAACTGCTCTAAAGTCGAAGTCCTTGTAGCGCGTCGCG
CCTTTTTTGGATATGAGCCC

Downstream 100 bases:

>100_bases
CTGGCCTGCGATGACTGAGCCGCTATGTCCCGCACTGAGCAGACTGTGGTCGTCCGAGCCGGACGGCGGCATGTCGCTGC
AACTCTCGGCCAGCATTGCG

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTLTQLRYLVAIADAELNITLAAGRVHATQPGLSKQLKQLEDELGFLLFVRKGRSLDGATPAGVEVIERARAVLSEVNNI
RTYAANQRRESQGQLTLTTTHTQARFVLPPAVAQIKNAYPQVSVHLQQAAESAALDLLGQGDADIAVVSTAGGEPSAGIA
VPLYRWRRLVVVPRGHALDHPRTAPEMHALSEHPLISYDSSTRPGSSLQRAFAQMGLEPSIALTALDADLIKTYVRAGLG
VGLVAEMAVNAFDKDLRAWPAPAPIAECIAWAVLPRDRVLRDYALDLVHVLAPQIDKRDLRRVLDGNQAPNWPLPPTWES
LTQTITS

Sequences:

>Translated_327_residues
MTLTQLRYLVAIADAELNITLAAGRVHATQPGLSKQLKQLEDELGFLLFVRKGRSLDGATPAGVEVIERARAVLSEVNNI
RTYAANQRRESQGQLTLTTTHTQARFVLPPAVAQIKNAYPQVSVHLQQAAESAALDLLGQGDADIAVVSTAGGEPSAGIA
VPLYRWRRLVVVPRGHALDHPRTAPEMHALSEHPLISYDSSTRPGSSLQRAFAQMGLEPSIALTALDADLIKTYVRAGLG
VGLVAEMAVNAFDKDLRAWPAPAPIAECIAWAVLPRDRVLRDYALDLVHVLAPQIDKRDLRRVLDGNQAPNWPLPPTWES
LTQTITS
>Mature_326_residues
TLTQLRYLVAIADAELNITLAAGRVHATQPGLSKQLKQLEDELGFLLFVRKGRSLDGATPAGVEVIERARAVLSEVNNIR
TYAANQRRESQGQLTLTTTHTQARFVLPPAVAQIKNAYPQVSVHLQQAAESAALDLLGQGDADIAVVSTAGGEPSAGIAV
PLYRWRRLVVVPRGHALDHPRTAPEMHALSEHPLISYDSSTRPGSSLQRAFAQMGLEPSIALTALDADLIKTYVRAGLGV
GLVAEMAVNAFDKDLRAWPAPAPIAECIAWAVLPRDRVLRDYALDLVHVLAPQIDKRDLRRVLDGNQAPNWPLPPTWESL
TQTITS

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=301, Percent_Identity=38.5382059800665, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1788296, Length=311, Percent_Identity=32.1543408360129, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1790399, Length=249, Percent_Identity=27.710843373494, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI2367136, Length=244, Percent_Identity=27.4590163934426, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787806, Length=177, Percent_Identity=31.0734463276836, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=28.4246575342466, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI157672245, Length=230, Percent_Identity=27.3913043478261, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787879, Length=300, Percent_Identity=24.6666666666667, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790262, Length=241, Percent_Identity=28.6307053941909, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35489; Mature: 35358

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTQLRYLVAIADAELNITLAAGRVHATQPGLSKQLKQLEDELGFLLFVRKGRSLDGAT
CCHHHHHHHHEEECCCEEEEEEECEEECCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCC
PAGVEVIERARAVLSEVNNIRTYAANQRRESQGQLTLTTTHTQARFVLPPAVAQIKNAYP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEECCHHHHHHHHCCC
QVSVHLQQAAESAALDLLGQGDADIAVVSTAGGEPSAGIAVPLYRWRRLVVVPRGHALDH
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEHHHCEEEEEEECCCCCCC
PRTAPEMHALSEHPLISYDSSTRPGSSLQRAFAQMGLEPSIALTALDADLIKTYVRAGLG
CCCCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCC
VGLVAEMAVNAFDKDLRAWPAPAPIAECIAWAVLPRDRVLRDYALDLVHVLAPQIDKRDL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
RRVLDGNQAPNWPLPPTWESLTQTITS
HHHHCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TLTQLRYLVAIADAELNITLAAGRVHATQPGLSKQLKQLEDELGFLLFVRKGRSLDGAT
CHHHHHHHHEEECCCEEEEEEECEEECCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCC
PAGVEVIERARAVLSEVNNIRTYAANQRRESQGQLTLTTTHTQARFVLPPAVAQIKNAYP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEECCHHHHHHHHCCC
QVSVHLQQAAESAALDLLGQGDADIAVVSTAGGEPSAGIAVPLYRWRRLVVVPRGHALDH
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEHHHCEEEEEEECCCCCCC
PRTAPEMHALSEHPLISYDSSTRPGSSLQRAFAQMGLEPSIALTALDADLIKTYVRAGLG
CCCCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCC
VGLVAEMAVNAFDKDLRAWPAPAPIAECIAWAVLPRDRVLRDYALDLVHVLAPQIDKRDL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
RRVLDGNQAPNWPLPPTWESLTQTITS
HHHHCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]