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The map label for this gene is parE [H]

Identifier: 84624476

GI number: 84624476

Start: 3167240

End: 3169129

Strand: Direct

Name: parE [H]

Synonym: XOO_2819

Alternate gene names: 84624476

Gene position: 3167240-3169129 (Clockwise)

Preceding gene: 84624466

Following gene: 84624479

Centisome position: 64.11

GC content: 63.23

Gene sequence:

>1890_bases
ATGAACACCCGTTATAACGCCGCCGATATTGAAGTGCTGTCGGGCCTGGACCCGGTCAAACGCCGTCCTGGCATGTACAC
CGACACCGCGCGCCCGAACCATCTGGCGCAGGAAGTGATCGACAACTCGGTCGACGAGGCGCTGGCCGGTCACGCCAAGC
AGATCGAAGTCACCTTGTACAAGGACGGCAGCTGCGAGGTGTCCGACGACGGCCGCGGCATGCCGGTGGACATCCATCCG
GAAGAGAAGATTCCGGGTGTGGAGTTGATCCTGACCCGGCTGCATGCCGGCGGCAAGTTCAACAACCGCAATTACACCTT
CTCCGGCGGCCTGCATGGCGTGGGTGTGAGCGTGGTCAATGCGTTGTCGACCAAGGTCGAGCTGTTCATCAAGCGCGAAG
GCAGCGAACACCGCATGGAGTTCCGCGACGGCAATGCCGCGTCCAAGCTGGAAGTGGTCGGCACGGTCGGCAAGAAAAAC
ACCGGCACGCGGCTGCGTTTCTGGGCCGACCCCAAGTATTTCGATACGCCCAAATTCAACGTGCGTGCGCTGCGGCATCT
GCTGCGCGCCAAGGCGGTGCTGTGCCCCGGCCTGACCGTGAAGCTGCACGACGAAGCCACCGGCGAGCAGGACAGCTGGT
ATTTCGAAAACGGCCTGCGCGATTACCTCAAAGGCGAGATGGCCGAGCACGAGATGCTGCCGGCCGACCTGTTCGTCGGT
AGCCTGAAAAAAGACACCGAAATCGTCGACTGGGCCGCAGGCTGGGTGCCGGAAGGCGAGCTGGTGCAGGAAAGCTACGT
CAACCTGATCCCCACCGCGCAGCACGGCACCCACGTCAACGGCCTGCGGTCGGGCCTGACCGATGCGCTGCGCGAGTTCT
GCGATTTCCGCAATCTGCTGCCACGCGGCGTCAAGCTGGCGCCGGAAGACGTGTGGGACCGGGTCACCTTCGTGCTCAGC
CTGAAGATGACCGACCCGCAATTCTCCGGGCAGACCAAGGAACGCCTGTCCTCGCGTCAGGCCGCCGGCTTCATCGAAGG
TGCTGCGCACGATGCCTTCAGCCTGTATCTGAATCAGAACGTGGAAATCGGCGAGAAGATCGCGCAGATCGCCATCGACC
GCGCCAGCGCACGGTTGAAGACCGAGAAGCAGATCGTCCGCAAGAAGGTCACCCAGGGCCCGGCCCTGCCCGGCAAGCTG
GCCGATTGCATCAGCCAGGATCTGTCCCGTACCGAATTGTTCCTGGTCGAAGGCGATTCGGCCGGTGGCTCGGCCAAGCA
GGCGCGCGACAAGGACTTCCAGGCGATCCTGCCGCTGCGTGGCAAGATCCTCAATACCTGGGAAGTGGCTTCCGGCAGCG
TGCTGGCCTCCGAAGAAGTGCATAACTTGGCGATCGCGATCGGCTGCGACCCGGGCAAGGACGACATCACCGGGCTGCGC
TACGGCAAGGTGGTGATCCTGGCCGACGCCGACTCCGACGGCCTGCATATCGCCACGCTGCTGACGGCGCTGTTCCTGCA
GCACTTCCCCGCGCTGGTGGCCGCCGGCCACGTGTTCGTGGCGATGCCGCCGCTGTTCCGCGTGGACGTGGGCAAGCAGG
TGTTCTACGCGCTGGACGAAGAAGAAAAGACCACGCTGCTGGACAAGATCGCGCGCGAGAAGATGAAGGGCCAGATCAGC
GTCACCCGCTTCAAGGGCCTGGGCGAAATGAATCCGCAGCAGCTGCGCGAATCCACCATCCACCCGGACACACGACGGCT
GGTGCAGCTGACCATCGATGATGGCGAGCAGACGCGTTTGTTGATGGACATGCTGCTGGCCAAGAAGCGCGCAAGCGACC
GCAAGCAGTGGCTGGAAACCAAGGGCGATCTGGCTTCGCTGGAAGTTTGA

Upstream 100 bases:

>100_bases
GGTATCCACTGGCACCCAGCCCTGCACTGAACGCCTGTCGCGTGCAAAAAATCGTCCCAGCCCCCACACTTGCGCGTCGT
TTCACGTGCCAACGAGATGC

Downstream 100 bases:

>100_bases
AACCCCCTAAGAACCTGTTTACGATCTCCTGAGCAGCAGTGCCAGGAACGCCAGGTGGATGAACTGCAAGCTGGTATTGA
GCCTTCGCTCGCAGTTCTTC

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MNTRYNAADIEVLSGLDPVKRRPGMYTDTARPNHLAQEVIDNSVDEALAGHAKQIEVTLYKDGSCEVSDDGRGMPVDIHP
EEKIPGVELILTRLHAGGKFNNRNYTFSGGLHGVGVSVVNALSTKVELFIKREGSEHRMEFRDGNAASKLEVVGTVGKKN
TGTRLRFWADPKYFDTPKFNVRALRHLLRAKAVLCPGLTVKLHDEATGEQDSWYFENGLRDYLKGEMAEHEMLPADLFVG
SLKKDTEIVDWAAGWVPEGELVQESYVNLIPTAQHGTHVNGLRSGLTDALREFCDFRNLLPRGVKLAPEDVWDRVTFVLS
LKMTDPQFSGQTKERLSSRQAAGFIEGAAHDAFSLYLNQNVEIGEKIAQIAIDRASARLKTEKQIVRKKVTQGPALPGKL
ADCISQDLSRTELFLVEGDSAGGSAKQARDKDFQAILPLRGKILNTWEVASGSVLASEEVHNLAIAIGCDPGKDDITGLR
YGKVVILADADSDGLHIATLLTALFLQHFPALVAAGHVFVAMPPLFRVDVGKQVFYALDEEEKTTLLDKIAREKMKGQIS
VTRFKGLGEMNPQQLRESTIHPDTRRLVQLTIDDGEQTRLLMDMLLAKKRASDRKQWLETKGDLASLEV

Sequences:

>Translated_629_residues
MNTRYNAADIEVLSGLDPVKRRPGMYTDTARPNHLAQEVIDNSVDEALAGHAKQIEVTLYKDGSCEVSDDGRGMPVDIHP
EEKIPGVELILTRLHAGGKFNNRNYTFSGGLHGVGVSVVNALSTKVELFIKREGSEHRMEFRDGNAASKLEVVGTVGKKN
TGTRLRFWADPKYFDTPKFNVRALRHLLRAKAVLCPGLTVKLHDEATGEQDSWYFENGLRDYLKGEMAEHEMLPADLFVG
SLKKDTEIVDWAAGWVPEGELVQESYVNLIPTAQHGTHVNGLRSGLTDALREFCDFRNLLPRGVKLAPEDVWDRVTFVLS
LKMTDPQFSGQTKERLSSRQAAGFIEGAAHDAFSLYLNQNVEIGEKIAQIAIDRASARLKTEKQIVRKKVTQGPALPGKL
ADCISQDLSRTELFLVEGDSAGGSAKQARDKDFQAILPLRGKILNTWEVASGSVLASEEVHNLAIAIGCDPGKDDITGLR
YGKVVILADADSDGLHIATLLTALFLQHFPALVAAGHVFVAMPPLFRVDVGKQVFYALDEEEKTTLLDKIAREKMKGQIS
VTRFKGLGEMNPQQLRESTIHPDTRRLVQLTIDDGEQTRLLMDMLLAKKRASDRKQWLETKGDLASLEV
>Mature_629_residues
MNTRYNAADIEVLSGLDPVKRRPGMYTDTARPNHLAQEVIDNSVDEALAGHAKQIEVTLYKDGSCEVSDDGRGMPVDIHP
EEKIPGVELILTRLHAGGKFNNRNYTFSGGLHGVGVSVVNALSTKVELFIKREGSEHRMEFRDGNAASKLEVVGTVGKKN
TGTRLRFWADPKYFDTPKFNVRALRHLLRAKAVLCPGLTVKLHDEATGEQDSWYFENGLRDYLKGEMAEHEMLPADLFVG
SLKKDTEIVDWAAGWVPEGELVQESYVNLIPTAQHGTHVNGLRSGLTDALREFCDFRNLLPRGVKLAPEDVWDRVTFVLS
LKMTDPQFSGQTKERLSSRQAAGFIEGAAHDAFSLYLNQNVEIGEKIAQIAIDRASARLKTEKQIVRKKVTQGPALPGKL
ADCISQDLSRTELFLVEGDSAGGSAKQARDKDFQAILPLRGKILNTWEVASGSVLASEEVHNLAIAIGCDPGKDDITGLR
YGKVVILADADSDGLHIATLLTALFLQHFPALVAAGHVFVAMPPLFRVDVGKQVFYALDEEEKTTLLDKIAREKMKGQIS
VTRFKGLGEMNPQQLRESTIHPDTRRLVQLTIDDGEQTRLLMDMLLAKKRASDRKQWLETKGDLASLEV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=624, Percent_Identity=24.8397435897436, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI19913408, Length=581, Percent_Identity=24.2685025817556, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1789408, Length=632, Percent_Identity=64.2405063291139, Blast_Score=867, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=552, Percent_Identity=40.7608695652174, Blast_Score=395, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17535065, Length=623, Percent_Identity=24.5585874799358, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI212645845, Length=591, Percent_Identity=23.3502538071066, Blast_Score=112, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6324241, Length=636, Percent_Identity=23.5849056603774, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17136538, Length=590, Percent_Identity=25.2542372881356, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.- [C]

Molecular weight: Translated: 69553; Mature: 69553

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTRYNAADIEVLSGLDPVKRRPGMYTDTARPNHLAQEVIDNSVDEALAGHAKQIEVTLY
CCCCCCCCHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHCCCCEEEEEEEE
KDGSCEVSDDGRGMPVDIHPEEKIPGVELILTRLHAGGKFNNRNYTFSGGLHGVGVSVVN
ECCCEEECCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHH
ALSTKVELFIKREGSEHRMEFRDGNAASKLEVVGTVGKKNTGTRLRFWADPKYFDTPKFN
HHHHEEEEEEEECCCHHCEEECCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCC
VRALRHLLRAKAVLCPGLTVKLHDEATGEQDSWYFENGLRDYLKGEMAEHEMLPADLFVG
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHC
SLKKDTEIVDWAAGWVPEGELVQESYVNLIPTAQHGTHVNGLRSGLTDALREFCDFRNLL
CCCCCCCEEEHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
PRGVKLAPEDVWDRVTFVLSLKMTDPQFSGQTKERLSSRQAAGFIEGAAHDAFSLYLNQN
CCCCCCCCHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHCCHHHCCCCCEEEEEECCC
VEIGEKIAQIAIDRASARLKTEKQIVRKKVTQGPALPGKLADCISQDLSRTELFLVEGDS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCC
AGGSAKQARDKDFQAILPLRGKILNTWEVASGSVLASEEVHNLAIAIGCDPGKDDITGLR
CCCCCHHHCCCCCCEECCCCCCEEEEEECCCCCEEECCCCCEEEEEEECCCCCCCCCCEE
YGKVVILADADSDGLHIATLLTALFLQHFPALVAAGHVFVAMPPLFRVDVGKQVFYALDE
ECEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCEEEEECC
EEKTTLLDKIAREKMKGQISVTRFKGLGEMNPQQLRESTIHPDTRRLVQLTIDDGEQTRL
CHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCHHHH
LMDMLLAKKRASDRKQWLETKGDLASLEV
HHHHHHHHHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure
MNTRYNAADIEVLSGLDPVKRRPGMYTDTARPNHLAQEVIDNSVDEALAGHAKQIEVTLY
CCCCCCCCHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHCCCCEEEEEEEE
KDGSCEVSDDGRGMPVDIHPEEKIPGVELILTRLHAGGKFNNRNYTFSGGLHGVGVSVVN
ECCCEEECCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHH
ALSTKVELFIKREGSEHRMEFRDGNAASKLEVVGTVGKKNTGTRLRFWADPKYFDTPKFN
HHHHEEEEEEEECCCHHCEEECCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCC
VRALRHLLRAKAVLCPGLTVKLHDEATGEQDSWYFENGLRDYLKGEMAEHEMLPADLFVG
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHC
SLKKDTEIVDWAAGWVPEGELVQESYVNLIPTAQHGTHVNGLRSGLTDALREFCDFRNLL
CCCCCCCEEEHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
PRGVKLAPEDVWDRVTFVLSLKMTDPQFSGQTKERLSSRQAAGFIEGAAHDAFSLYLNQN
CCCCCCCCHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHCCHHHCCCCCEEEEEECCC
VEIGEKIAQIAIDRASARLKTEKQIVRKKVTQGPALPGKLADCISQDLSRTELFLVEGDS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCC
AGGSAKQARDKDFQAILPLRGKILNTWEVASGSVLASEEVHNLAIAIGCDPGKDDITGLR
CCCCCHHHCCCCCCEECCCCCCEEEEEECCCCCEEECCCCCEEEEEEECCCCCCCCCCEE
YGKVVILADADSDGLHIATLLTALFLQHFPALVAAGHVFVAMPPLFRVDVGKQVFYALDE
ECEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCEEEEECC
EEKTTLLDKIAREKMKGQISVTRFKGLGEMNPQQLRESTIHPDTRRLVQLTIDDGEQTRL
CHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCHHHH
LMDMLLAKKRASDRKQWLETKGDLASLEV
HHHHHHHHHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]