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The map label for this gene is pleD [H]

Identifier: 84624284

GI number: 84624284

Start: 2931045

End: 2932343

Strand: Direct

Name: pleD [H]

Synonym: XOO_2627

Alternate gene names: 84624284

Gene position: 2931045-2932343 (Clockwise)

Preceding gene: 84624283

Following gene: 84624290

Centisome position: 59.33

GC content: 61.51

Gene sequence:

>1299_bases
ATGTCCCCAAGTAAAGTCCATCGCGATAGTGAAAGCGTCTTTCTGCCGCAGCGTGTGCTGTTGGTGGAGAATTCGCGCAC
GTTCACCAGCATGCTGCGCGAAGCCATCGAGCAACGCGTGGAACTTTCAGTCACCGTCGTGTCGACCCTGGCCGAAGCTG
CGCGTGTGCTTGACGAAGAGGATGGCTGGTTTCTGGTCCTGACCGGCCTGGTGCTGGTCGACGGCGACCGCGACAAGGTC
GTGGAATTCTTTCTCTCGCGCAAACTGCCCACCGTGGTGGTCAGCGGCGTGTACGACGAAGACCTGCGTCAGCGCGTGCT
ACAGCAGCAGATCATCGATTACGTACTGAAAAACGCACCGGGCAGCATCGAATACCTGGCCTGGCTGGTACAGCGTTTGG
AGCGCAATCGCCGGATCGCCGCCCTGGTGGTGGACGATTCGCTGTCGGCACGCACCTATGCCGGCGCGCTGCTGTCGATG
TACGGCTACCGCGTGGTGCTGGCAGCCGACGGCCCTGCCGGGCTGGAAGCAATCGAACGCGACCCGAGCATTCGCCTGAC
CATCGTCGACCAGGAAATGCCTGGCATGGATGGCGTGGAATTTACCCGCCGGCTACGTGCGATCCGCTCGCGCGACAAGG
TGGCGCTGATCGGTATTTCCGGTAACAGCGATTCGTCGCTGATCCCGCGCTTTTTGAAGAACGGCGCCAACGATTTCCTG
CGCAAGCCGTTTTCGCGCGAAGAATTCTTCTGCCGCGTGTCGCAGAACGTGGACCAGCTGGAACTGATTGGCACCCTGCA
GGATCTGGCTACCCGCGACTTCCTCACCGGCTTACCCAACCGGCGTTACTTTCTGGAACAAAGCCAGCGTGTCATTACGA
AACTGTTGTCGCAGGACCAGACGGTGACTGCGGCGATGGTGGATATCGATCACTTCAAACACATCAACGACACCTGGGGC
CACGACGTTGGCGACCAAGCGCTGCGTGCGGTGGCCGCTTCGGTCTCCGGGCATGCCCGGCCGCAGGACCTGGTGGCGCG
CTTCGGCGGCGAAGAATTCTGTCTGCTGGTGCCCGGCCTGGATGCGGCCAACGCCACCAGCTATTTCGAGACGTTGCGCG
AACGCATCAGCGCGCTGCGCGTGCGCGTGGGCGAAGAGACGCTCAGCATGACGGTCAGCATCGGCGTGTGCATCGCCAGC
GAAAGCGACCAGTCGCTGCATTACCTGTTGAACGAAGCAGACAAGTACCTGTATCTGGCCAAGGCCGGCGGCCGTAACCA
GGTGCGTCTGGCAGGCTGA

Upstream 100 bases:

>100_bases
CGCGATGCACTGTGGTGACCGGCGCGTTGCAGCCACGTGCTCAAGTTTTTGCCAGCAGGGCCGTTAGCTGACCTGACGGT
TCGATCCAGACGGCCAACTG

Downstream 100 bases:

>100_bases
TCCTTCCGTCATCTGCTGGCCCAGGCAGGCCGTTGCAGATGACGCGCTTCATCGCATGCCTTGGCTCAGATCAACGCACG
GCCGACGCGTGCGGTTCACT

Product: transcriptional regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSPSKVHRDSESVFLPQRVLLVENSRTFTSMLREAIEQRVELSVTVVSTLAEAARVLDEEDGWFLVLTGLVLVDGDRDKV
VEFFLSRKLPTVVVSGVYDEDLRQRVLQQQIIDYVLKNAPGSIEYLAWLVQRLERNRRIAALVVDDSLSARTYAGALLSM
YGYRVVLAADGPAGLEAIERDPSIRLTIVDQEMPGMDGVEFTRRLRAIRSRDKVALIGISGNSDSSLIPRFLKNGANDFL
RKPFSREEFFCRVSQNVDQLELIGTLQDLATRDFLTGLPNRRYFLEQSQRVITKLLSQDQTVTAAMVDIDHFKHINDTWG
HDVGDQALRAVAASVSGHARPQDLVARFGGEEFCLLVPGLDAANATSYFETLRERISALRVRVGEETLSMTVSIGVCIAS
ESDQSLHYLLNEADKYLYLAKAGGRNQVRLAG

Sequences:

>Translated_432_residues
MSPSKVHRDSESVFLPQRVLLVENSRTFTSMLREAIEQRVELSVTVVSTLAEAARVLDEEDGWFLVLTGLVLVDGDRDKV
VEFFLSRKLPTVVVSGVYDEDLRQRVLQQQIIDYVLKNAPGSIEYLAWLVQRLERNRRIAALVVDDSLSARTYAGALLSM
YGYRVVLAADGPAGLEAIERDPSIRLTIVDQEMPGMDGVEFTRRLRAIRSRDKVALIGISGNSDSSLIPRFLKNGANDFL
RKPFSREEFFCRVSQNVDQLELIGTLQDLATRDFLTGLPNRRYFLEQSQRVITKLLSQDQTVTAAMVDIDHFKHINDTWG
HDVGDQALRAVAASVSGHARPQDLVARFGGEEFCLLVPGLDAANATSYFETLRERISALRVRVGEETLSMTVSIGVCIAS
ESDQSLHYLLNEADKYLYLAKAGGRNQVRLAG
>Mature_431_residues
SPSKVHRDSESVFLPQRVLLVENSRTFTSMLREAIEQRVELSVTVVSTLAEAARVLDEEDGWFLVLTGLVLVDGDRDKVV
EFFLSRKLPTVVVSGVYDEDLRQRVLQQQIIDYVLKNAPGSIEYLAWLVQRLERNRRIAALVVDDSLSARTYAGALLSMY
GYRVVLAADGPAGLEAIERDPSIRLTIVDQEMPGMDGVEFTRRLRAIRSRDKVALIGISGNSDSSLIPRFLKNGANDFLR
KPFSREEFFCRVSQNVDQLELIGTLQDLATRDFLTGLPNRRYFLEQSQRVITKLLSQDQTVTAAMVDIDHFKHINDTWGH
DVGDQALRAVAASVSGHARPQDLVARFGGEEFCLLVPGLDAANATSYFETLRERISALRVRVGEETLSMTVSIGVCIASE
SDQSLHYLLNEADKYLYLAKAGGRNQVRLAG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=168, Percent_Identity=37.5, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=37.1951219512195, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=38.8888888888889, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=35.5029585798817, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=36.3636363636364, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI145693134, Length=192, Percent_Identity=30.7291666666667, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787802, Length=203, Percent_Identity=31.5270935960591, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787541, Length=197, Percent_Identity=30.4568527918782, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788381, Length=159, Percent_Identity=28.9308176100629, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081974, Length=149, Percent_Identity=30.2013422818792, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=104, Percent_Identity=37.5, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 48253; Mature: 48121

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSKVHRDSESVFLPQRVLLVENSRTFTSMLREAIEQRVELSVTVVSTLAEAARVLDEE
CCCCCCCCCCCCEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGWFLVLTGLVLVDGDRDKVVEFFLSRKLPTVVVSGVYDEDLRQRVLQQQIIDYVLKNAP
CCEEEEEEEEEEEECCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCC
GSIEYLAWLVQRLERNRRIAALVVDDSLSARTYAGALLSMYGYRVVLAADGPAGLEAIER
CHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHCC
DPSIRLTIVDQEMPGMDGVEFTRRLRAIRSRDKVALIGISGNSDSSLIPRFLKNGANDFL
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCHHHH
RKPFSREEFFCRVSQNVDQLELIGTLQDLATRDFLTGLPNRRYFLEQSQRVITKLLSQDQ
HCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
TVTAAMVDIDHFKHINDTWGHDVGDQALRAVAASVSGHARPQDLVARFGGEEFCLLVPGL
EEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCC
DAANATSYFETLRERISALRVRVGEETLSMTVSIGVCIASESDQSLHYLLNEADKYLYLA
CCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEEEECCCCCHHHHHHHCCCCEEEEE
KAGGRNQVRLAG
ECCCCCCEEECC
>Mature Secondary Structure 
SPSKVHRDSESVFLPQRVLLVENSRTFTSMLREAIEQRVELSVTVVSTLAEAARVLDEE
CCCCCCCCCCCEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGWFLVLTGLVLVDGDRDKVVEFFLSRKLPTVVVSGVYDEDLRQRVLQQQIIDYVLKNAP
CCEEEEEEEEEEEECCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCC
GSIEYLAWLVQRLERNRRIAALVVDDSLSARTYAGALLSMYGYRVVLAADGPAGLEAIER
CHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHCC
DPSIRLTIVDQEMPGMDGVEFTRRLRAIRSRDKVALIGISGNSDSSLIPRFLKNGANDFL
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCHHHH
RKPFSREEFFCRVSQNVDQLELIGTLQDLATRDFLTGLPNRRYFLEQSQRVITKLLSQDQ
HCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
TVTAAMVDIDHFKHINDTWGHDVGDQALRAVAASVSGHARPQDLVARFGGEEFCLLVPGL
EEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCC
DAANATSYFETLRERISALRVRVGEETLSMTVSIGVCIASESDQSLHYLLNEADKYLYLA
CCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEEEECCCCCHHHHHHHCCCCEEEEE
KAGGRNQVRLAG
ECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA