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The map label for this gene is nreB [H]

Identifier: 84624078

GI number: 84624078

Start: 2692573

End: 2694705

Strand: Direct

Name: nreB [H]

Synonym: XOO_2421

Alternate gene names: 84624078

Gene position: 2692573-2694705 (Clockwise)

Preceding gene: 84624076

Following gene: 84624079

Centisome position: 54.5

GC content: 65.21

Gene sequence:

>2133_bases
ATGGCTGCATCACCAGTATCCAAGGTCGTCTACGGATCCATGCAACAGCCCGTCGCCGCGCCCGCGTTTTCGTCCGCTCC
CGTCTCCTGTCTCCGCCTCGCCGAGCGCCTGCAGCAAGGCCTGTGGACCCTGACTGGTCTGTATCTGCTGATTGGCGTGG
TCTGGCTGCTGCTGGGCAATCGGCTGCTCAACCTGGCCGGTGCCGCCGTGCCGGAGCGCTGGTCGGACGCGAGCTTTCTG
GTGCTGTCCAGCATCGTCATCCACCTGGTGCTGCGCCGCTTTGCCACGTTGATCGTGGACGAGTACGCACAACTGGCGCA
GTCCGAAGCCTTGCTGCAGGCCAAGTTTATGGCCCTGCCCAGCCCTGCCTTCATCTACGACATGGCTACGATGCGCATCC
TCGACGCGAATCCGGTGGCACTGGAATTCTTCGGATGGGAACGTGACGAATTCTTGCAGCAGACGCTGCAGGCGATCTGG
CCCAACGCCGATGGTGACCGGCTAGAAGAGATGATCACGCAGATCCGCGGCAAGGGCGACGCCACCTGCGTGTTGAACGA
AGACCTGCTGACGCGCAGCGGGCCACGGCATGTCGAGGTGCGTAGCAACCAGCTGCACCTGTCCAGCGGCCCTGCCCGCC
TGGTGGTCACCGTGGACCGCAACGAAGAGCGCCAGGCGCAGCATCTGCGCGACGAGGCGCTTGAGCGCCTGGAGGAAGCG
CAGAGCATCGCGCGGCTGGGCTCCTGGCAACTGGATCCCACGACCGGCCTGGGACGCTATTCGCCGGCGGTCTATCGCCT
GCTGGGGCGCAGCGTGCCGATGCACCGTCGCGAGCATCGGCTGGAAGAATTGCTGACCGCCTCCGACAGCCCCACCCAGG
CGCGTATCGAACGCATGATCGAAGACATGTGCACCGGTGGCGAGGTGCAGATCGATGTGCTGTTGCCTCTGACCGGTGGC
GATTCGCAACCGCGCACCGTGCACCTGCGCGCCGAACGCGTAACCAGCCCCAGCGGCGCGCGCTATGTGCACGGCACTTT
GCAGGACGTGAGCGAACGCGAGCAATCGCGCCGGCTGCTGCGCGAACGCGAGGAGCAGTTTCGCGAACTCGTGCGCGTGT
TGCCCGACGGTGTGCTCATCCTCGCCGACGAGCATGTGATGTACGCCAACGCGGCCGGCTCCGCTCAGTTCGGCTTTCAG
GGCGAAACCATCCTGGGCGAGCCGCTGCAGACCCTGGTCGGCAATAACGACTTGCCGGTGGTGCGCGAATACCTGCGTGC
AGGCAGCGATCGCAACGAGCCGGTTTCCAGCGCACGCGCGATGCGCCGCCGCGATGGCAGTACCTTCTATGCTGGCCTCT
CCGCCGGCGATATCCGCTATGGCGGGCGAAGCTGCAAGTTGCTGGTCGTGCGCGACCTGAGCGAACCGGAGCGCATGCGC
GATGCCCTGGCTGAAAGCAATGCCGAACTGCAGGCGATGGCCAAGCGGCTGTTCTCGTTGCAGGAAGACGAACGCCGCGC
CATCTCGCGCGATCTGCACGACGACATCGGGCAGGCGATCACTGCGATGAAGCTGTCCGCGCATGCGGCGTTGGATGAGC
AGGACGCAGAGGCGCGCCGCGAGGACATGCTGGAAGTGGTGTCGCTGGCCGACAGCACGGTGACCAAGCTGCGCAATCTG
TCGATGCTGCTGCGTCCGCCACAACTCGATGCCTTGGGCCTAGAGGCCGCTTTGCGTTGGCAGGCGTCGATGCTGTTCCG
CGCCTCGCAGATACGGCTGGAGCTGGACATTCAGGCGCTTGAAGAACGGCCGGGCAACGAGATCGAGCAAGCCTGCTTCC
GGATCGCACAGGAGAGCCTCACTAATGCGCTGCGGCACGCCTGCGCGGGCGAAGTGCACCTGCGTCTGCATTCGATCGAC
AGCGACAGTTTCCGGCTGGAAGTCAGCGATGATGGCGATGGCTTCGAGCCGGAGGGCCCACGTGGCCTGGGCCTGATCGT
GATGCGTGAGCGTGCGCAAACCGTGGGCGGCACGCTCGCGATAGAATCGGCACCCGGAGCAGGCACGCGCGTGACGTTGC
GCCTGCCCTATCACTCGGTCGGCGAGTCCGTCCACGACGATGGTGGACGTTGA

Upstream 100 bases:

>100_bases
CGCCTCCGGCCTTCGGAGTCTCAATGGATAACGGCCGTGCGGCCGATTAGTTGAGCCACCTGTTTCGAGCTGGACCGAAC
CAGCCCGAGTTACGGCTAAG

Downstream 100 bases:

>100_bases
GTCATGTGGAACCCTGTTAGTCCGCCCGGACTGGAGGTCGTCAAGCCCACACGGCTTGGCGCGGGCAACCCCGAATTGCT
GCATTTAGTCGATGCGGCCG

Product: HrpX related protein

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MAASPVSKVVYGSMQQPVAAPAFSSAPVSCLRLAERLQQGLWTLTGLYLLIGVVWLLLGNRLLNLAGAAVPERWSDASFL
VLSSIVIHLVLRRFATLIVDEYAQLAQSEALLQAKFMALPSPAFIYDMATMRILDANPVALEFFGWERDEFLQQTLQAIW
PNADGDRLEEMITQIRGKGDATCVLNEDLLTRSGPRHVEVRSNQLHLSSGPARLVVTVDRNEERQAQHLRDEALERLEEA
QSIARLGSWQLDPTTGLGRYSPAVYRLLGRSVPMHRREHRLEELLTASDSPTQARIERMIEDMCTGGEVQIDVLLPLTGG
DSQPRTVHLRAERVTSPSGARYVHGTLQDVSEREQSRRLLREREEQFRELVRVLPDGVLILADEHVMYANAAGSAQFGFQ
GETILGEPLQTLVGNNDLPVVREYLRAGSDRNEPVSSARAMRRRDGSTFYAGLSAGDIRYGGRSCKLLVVRDLSEPERMR
DALAESNAELQAMAKRLFSLQEDERRAISRDLHDDIGQAITAMKLSAHAALDEQDAEARREDMLEVVSLADSTVTKLRNL
SMLLRPPQLDALGLEAALRWQASMLFRASQIRLELDIQALEERPGNEIEQACFRIAQESLTNALRHACAGEVHLRLHSID
SDSFRLEVSDDGDGFEPEGPRGLGLIVMRERAQTVGGTLAIESAPGAGTRVTLRLPYHSVGESVHDDGGR

Sequences:

>Translated_710_residues
MAASPVSKVVYGSMQQPVAAPAFSSAPVSCLRLAERLQQGLWTLTGLYLLIGVVWLLLGNRLLNLAGAAVPERWSDASFL
VLSSIVIHLVLRRFATLIVDEYAQLAQSEALLQAKFMALPSPAFIYDMATMRILDANPVALEFFGWERDEFLQQTLQAIW
PNADGDRLEEMITQIRGKGDATCVLNEDLLTRSGPRHVEVRSNQLHLSSGPARLVVTVDRNEERQAQHLRDEALERLEEA
QSIARLGSWQLDPTTGLGRYSPAVYRLLGRSVPMHRREHRLEELLTASDSPTQARIERMIEDMCTGGEVQIDVLLPLTGG
DSQPRTVHLRAERVTSPSGARYVHGTLQDVSEREQSRRLLREREEQFRELVRVLPDGVLILADEHVMYANAAGSAQFGFQ
GETILGEPLQTLVGNNDLPVVREYLRAGSDRNEPVSSARAMRRRDGSTFYAGLSAGDIRYGGRSCKLLVVRDLSEPERMR
DALAESNAELQAMAKRLFSLQEDERRAISRDLHDDIGQAITAMKLSAHAALDEQDAEARREDMLEVVSLADSTVTKLRNL
SMLLRPPQLDALGLEAALRWQASMLFRASQIRLELDIQALEERPGNEIEQACFRIAQESLTNALRHACAGEVHLRLHSID
SDSFRLEVSDDGDGFEPEGPRGLGLIVMRERAQTVGGTLAIESAPGAGTRVTLRLPYHSVGESVHDDGGR
>Mature_709_residues
AASPVSKVVYGSMQQPVAAPAFSSAPVSCLRLAERLQQGLWTLTGLYLLIGVVWLLLGNRLLNLAGAAVPERWSDASFLV
LSSIVIHLVLRRFATLIVDEYAQLAQSEALLQAKFMALPSPAFIYDMATMRILDANPVALEFFGWERDEFLQQTLQAIWP
NADGDRLEEMITQIRGKGDATCVLNEDLLTRSGPRHVEVRSNQLHLSSGPARLVVTVDRNEERQAQHLRDEALERLEEAQ
SIARLGSWQLDPTTGLGRYSPAVYRLLGRSVPMHRREHRLEELLTASDSPTQARIERMIEDMCTGGEVQIDVLLPLTGGD
SQPRTVHLRAERVTSPSGARYVHGTLQDVSEREQSRRLLREREEQFRELVRVLPDGVLILADEHVMYANAAGSAQFGFQG
ETILGEPLQTLVGNNDLPVVREYLRAGSDRNEPVSSARAMRRRDGSTFYAGLSAGDIRYGGRSCKLLVVRDLSEPERMRD
ALAESNAELQAMAKRLFSLQEDERRAISRDLHDDIGQAITAMKLSAHAALDEQDAEARREDMLEVVSLADSTVTKLRNLS
MLLRPPQLDALGLEAALRWQASMLFRASQIRLELDIQALEERPGNEIEQACFRIAQESLTNALRHACAGEVHLRLHSIDS
DSFRLEVSDDGDGFEPEGPRGLGLIVMRERAQTVGGTLAIESAPGAGTRVTLRLPYHSVGESVHDDGGR

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=239, Percent_Identity=35.5648535564854, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1787474, Length=257, Percent_Identity=28.0155642023346, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788812, Length=196, Percent_Identity=28.0612244897959, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 78755; Mature: 78624

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASPVSKVVYGSMQQPVAAPAFSSAPVSCLRLAERLQQGLWTLTGLYLLIGVVWLLLGN
CCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLNLAGAAVPERWSDASFLVLSSIVIHLVLRRFATLIVDEYAQLAQSEALLQAKFMALP
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPAFIYDMATMRILDANPVALEFFGWERDEFLQQTLQAIWPNADGDRLEEMITQIRGKGD
CCHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
ATCVLNEDLLTRSGPRHVEVRSNQLHLSSGPARLVVTVDRNEERQAQHLRDEALERLEEA
EEEEECCHHHHCCCCCEEEEECCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
QSIARLGSWQLDPTTGLGRYSPAVYRLLGRSVPMHRREHRLEELLTASDSPTQARIERMI
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
EDMCTGGEVQIDVLLPLTGGDSQPRTVHLRAERVTSPSGARYVHGTLQDVSEREQSRRLL
HHHCCCCCEEEEEEEEECCCCCCCCEEEEEEHHCCCCCCCEEEHHHHHHHHHHHHHHHHH
REREEQFRELVRVLPDGVLILADEHVMYANAAGSAQFGFQGETILGEPLQTLVGNNDLPV
HHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCHH
VREYLRAGSDRNEPVSSARAMRRRDGSTFYAGLSAGDIRYGGRSCKLLVVRDLSEPERMR
HHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEECCCCEEEEEEECCCCHHHHH
DALAESNAELQAMAKRLFSLQEDERRAISRDLHDDIGQAITAMKLSAHAALDEQDAEARR
HHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EDMLEVVSLADSTVTKLRNLSMLLRPPQLDALGLEAALRWQASMLFRASQIRLELDIQAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHH
EERPGNEIEQACFRIAQESLTNALRHACAGEVHLRLHSIDSDSFRLEVSDDGDGFEPEGP
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCC
RGLGLIVMRERAQTVGGTLAIESAPGAGTRVTLRLPYHSVGESVHDDGGR
CCCEEEEEEHHHHHCCCEEEEECCCCCCCEEEEECCHHHHCCCCCCCCCC
>Mature Secondary Structure 
AASPVSKVVYGSMQQPVAAPAFSSAPVSCLRLAERLQQGLWTLTGLYLLIGVVWLLLGN
CCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLNLAGAAVPERWSDASFLVLSSIVIHLVLRRFATLIVDEYAQLAQSEALLQAKFMALP
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPAFIYDMATMRILDANPVALEFFGWERDEFLQQTLQAIWPNADGDRLEEMITQIRGKGD
CCHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
ATCVLNEDLLTRSGPRHVEVRSNQLHLSSGPARLVVTVDRNEERQAQHLRDEALERLEEA
EEEEECCHHHHCCCCCEEEEECCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
QSIARLGSWQLDPTTGLGRYSPAVYRLLGRSVPMHRREHRLEELLTASDSPTQARIERMI
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
EDMCTGGEVQIDVLLPLTGGDSQPRTVHLRAERVTSPSGARYVHGTLQDVSEREQSRRLL
HHHCCCCCEEEEEEEEECCCCCCCCEEEEEEHHCCCCCCCEEEHHHHHHHHHHHHHHHHH
REREEQFRELVRVLPDGVLILADEHVMYANAAGSAQFGFQGETILGEPLQTLVGNNDLPV
HHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCHH
VREYLRAGSDRNEPVSSARAMRRRDGSTFYAGLSAGDIRYGGRSCKLLVVRDLSEPERMR
HHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEECCCCEEEEEEECCCCHHHHH
DALAESNAELQAMAKRLFSLQEDERRAISRDLHDDIGQAITAMKLSAHAALDEQDAEARR
HHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EDMLEVVSLADSTVTKLRNLSMLLRPPQLDALGLEAALRWQASMLFRASQIRLELDIQAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHH
EERPGNEIEQACFRIAQESLTNALRHACAGEVHLRLHSIDSDSFRLEVSDDGDGFEPEGP
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCC
RGLGLIVMRERAQTVGGTLAIESAPGAGTRVTLRLPYHSVGESVHDDGGR
CCCEEEEEEHHHHHCCCEEEEECCCCCCCEEEEECCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]