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The map label for this gene is nth [H]

Identifier: 84623982

GI number: 84623982

Start: 2585813

End: 2586523

Strand: Direct

Name: nth [H]

Synonym: XOO_2325

Alternate gene names: 84623982

Gene position: 2585813-2586523 (Clockwise)

Preceding gene: 84623981

Following gene: 84623987

Centisome position: 52.34

GC content: 62.87

Gene sequence:

>711_bases
ATGAACGCACCGCAGGCAACGCCACCAGCGCGTCGTGGCAGCAGCATGCGCAAGCCCGAGATACAGGAGATGTTCGAGCG
CCTGCGCGAACTCAATCCGCACCCGACCACCGAACTGGAATACACCACCCCGTTCGAGTTGTTGATCGCCGTGCTGTTGT
CGGCGCAGGCTACCGATGTGGGCGTCAACAAGGCCACCCGCAAGCTGTATCCGGTGGCTAACACACCGCGCGACATTCTC
GATCTCGGCGAAGAAGGCCTGAAGCGGTACATCGCCACCATCGGCTTGTTCAACGCCAAGGCCAAGAACGTCATCGCCAC
CTGTCGCATTCTGCTGGAGCGTTACGGCGGCGAAGTGCCGCACGACCGCGCCGCACTCGAGGCATTGCCCGGCGTCGGCC
GCAAAACCGCAAACGTGGTGCTCAACACCGCGTTCGGCGAGCCTGCGATGGCAGTGGACACGCATATCTTTCGCGTCTCC
AACCGCACCGGTCTGGCGCCGGGCAAGGACGTCCGCGTTGTCGAAGACAGGCTGGTCAAGGTGATTCCCGCCCAGTTTCT
TTACGACGCGCATCACTGGCTGATCCTGCACGGCCGCTACGTCTGCAAGGCACGCAAACCGGATTGCCCAGGCTGCGTGA
TCCACGATCTATGCCGCTACCGCGACAAGACGCCGCCGGCCCCTGAGCGGCCAACAGACACCAAGAGTTGA

Upstream 100 bases:

>100_bases
CCGCTCCGGCACCCAGCCAGCCGGCATTGCCGGCATTGCCGCCGCTGGCCGCCGCCATCCTGGCACGTGCCAAGGCCAAA
GCCGCCGGCGGAGCGCAGCC

Downstream 100 bases:

>100_bases
TGCAGCAGTGAAGCCGAAGCAACCACGCAACCGCGCGCCCTGATCCACAGCCATCAACGGCAAGGCCGCAAGCTTTTACG
CATCACGAATCCCTACTCCC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MNAPQATPPARRGSSMRKPEIQEMFERLRELNPHPTTELEYTTPFELLIAVLLSAQATDVGVNKATRKLYPVANTPRDIL
DLGEEGLKRYIATIGLFNAKAKNVIATCRILLERYGGEVPHDRAALEALPGVGRKTANVVLNTAFGEPAMAVDTHIFRVS
NRTGLAPGKDVRVVEDRLVKVIPAQFLYDAHHWLILHGRYVCKARKPDCPGCVIHDLCRYRDKTPPAPERPTDTKS

Sequences:

>Translated_236_residues
MNAPQATPPARRGSSMRKPEIQEMFERLRELNPHPTTELEYTTPFELLIAVLLSAQATDVGVNKATRKLYPVANTPRDIL
DLGEEGLKRYIATIGLFNAKAKNVIATCRILLERYGGEVPHDRAALEALPGVGRKTANVVLNTAFGEPAMAVDTHIFRVS
NRTGLAPGKDVRVVEDRLVKVIPAQFLYDAHHWLILHGRYVCKARKPDCPGCVIHDLCRYRDKTPPAPERPTDTKS
>Mature_236_residues
MNAPQATPPARRGSSMRKPEIQEMFERLRELNPHPTTELEYTTPFELLIAVLLSAQATDVGVNKATRKLYPVANTPRDIL
DLGEEGLKRYIATIGLFNAKAKNVIATCRILLERYGGEVPHDRAALEALPGVGRKTANVVLNTAFGEPAMAVDTHIFRVS
NRTGLAPGKDVRVVEDRLVKVIPAQFLYDAHHWLILHGRYVCKARKPDCPGCVIHDLCRYRDKTPPAPERPTDTKS

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=30.5084745762712, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=71.6346153846154, Blast_Score=315, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=31.638418079096, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=192, Percent_Identity=24.4791666666667, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=30, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26267; Mature: 26267

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPQATPPARRGSSMRKPEIQEMFERLRELNPHPTTELEYTTPFELLIAVLLSAQATDV
CCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHCCCCCC
GVNKATRKLYPVANTPRDILDLGEEGLKRYIATIGLFNAKAKNVIATCRILLERYGGEVP
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCC
HDRAALEALPGVGRKTANVVLNTAFGEPAMAVDTHIFRVSNRTGLAPGKDVRVVEDRLVK
CHHHHHHHCCCCCCCHHHEEEECCCCCCCHHHHHEEEEECCCCCCCCCCCHHHHHHHHHH
VIPAQFLYDAHHWLILHGRYVCKARKPDCPGCVIHDLCRYRDKTPPAPERPTDTKS
HHHHHHHHCCCEEEEEECEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAPQATPPARRGSSMRKPEIQEMFERLRELNPHPTTELEYTTPFELLIAVLLSAQATDV
CCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHCCCCCC
GVNKATRKLYPVANTPRDILDLGEEGLKRYIATIGLFNAKAKNVIATCRILLERYGGEVP
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCC
HDRAALEALPGVGRKTANVVLNTAFGEPAMAVDTHIFRVSNRTGLAPGKDVRVVEDRLVK
CHHHHHHHCCCCCCCHHHEEEECCCCCCCHHHHHEEEEECCCCCCCCCCCHHHHHHHHHH
VIPAQFLYDAHHWLILHGRYVCKARKPDCPGCVIHDLCRYRDKTPPAPERPTDTKS
HHHHHHHHCCCEEEEEECEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]