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The map label for this gene is ygfZ [C]

Identifier: 84623846

GI number: 84623846

Start: 2427170

End: 2428042

Strand: Direct

Name: ygfZ [C]

Synonym: XOO_2189

Alternate gene names: 84623846

Gene position: 2427170-2428042 (Clockwise)

Preceding gene: 84623837

Following gene: 84623848

Centisome position: 49.13

GC content: 64.72

Gene sequence:

>873_bases
GTGGCTGACAACCTGAATCTTATTCCGCAGGGTTTTGGCTCCCTGCAAGATATCCAATACGTCCGATTGGTCGGCGCGGA
TGCAGTGGCGTTCGCGCATGCCCAATTTGCCAATGACGTACAGGCGCTGGCGATTGGGCAGTGGCAGTGGAATGCGTGGC
TGACGGCGAAGGGGCGCGTGATTGCCATCTTCGCACTTCTGCGTGAGGACGATGCACACCTGCTGATGCTGTTGCCGGAC
GGCAACGCGGCCGAGATCGCCGCCCAACTCGGCCGTTTCGTCTTCCGCCGCAAGCTGAAAATCAGCACTGCCGCGCTGTT
TGCCTTTGGCGGCTTCGCAGCACCTGAACGCGCGCGCGCCGCGCAAGCCGATCTCGGAACGCAGCGTATCGTGCTGGATC
TGGGCAGCGCTGCGTTGCCGCGCACCTTGCTGCTGTACGCCGAAGAAGCATTGGCAGCGCCTATCGAAGCGCCGAGCGTA
GATGCGCAGTGGCGGCGCGCGGATCTGCAGTTGGGGCTCGCTCGCCTGGTCGAGGGCCAGCGCGAACAGTGGACGCCACA
ACAATTGGCACTCGACCGTTTGCAGGCCTACAGCGTCAAAAAAGGCTGCTACCCGGGTCAGGAGATCGTGGCGCGCACGC
ATTTCCTGGGCAAGGCCAAGCGCGCCCTGCAGCTGCTGGAAACCGATAGCGCGGTCGAGGCAGGCGATGCCGTCGCAATG
GACGGCGCCGCGATCGGCACAGTAGTGAGCGTGGCTGGCAACCTGGCGCTGGCAGTGTTGCCGTTGGAGCTGACACTGGA
TGCCGACACTCCCTTGCAGGCCGGTGCGCATGGCGCCCGGCCGCGCGCGATCGCGCCGGGGCTGGAGCGGTAA

Upstream 100 bases:

>100_bases
CCTGAGAAGAAGGCTGCGTCTCGGAATCCTGCGCTGGGGTGGGGGTTGGATGGCCTATCATAGTGCTCTCACAACTTGGC
GATTTTAGTCCTCCCCTTCC

Downstream 100 bases:

>100_bases
GCGCAGCGAAGAAACGTCGCGAGCCGTCGTCCGGTTGGCGTGGACGGCGCGGAGGAACCGTGGTGTACGCATGGTGCATG
CCGCCCCAGGATCCGGCCGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MADNLNLIPQGFGSLQDIQYVRLVGADAVAFAHAQFANDVQALAIGQWQWNAWLTAKGRVIAIFALLREDDAHLLMLLPD
GNAAEIAAQLGRFVFRRKLKISTAALFAFGGFAAPERARAAQADLGTQRIVLDLGSAALPRTLLLYAEEALAAPIEAPSV
DAQWRRADLQLGLARLVEGQREQWTPQQLALDRLQAYSVKKGCYPGQEIVARTHFLGKAKRALQLLETDSAVEAGDAVAM
DGAAIGTVVSVAGNLALAVLPLELTLDADTPLQAGAHGARPRAIAPGLER

Sequences:

>Translated_290_residues
MADNLNLIPQGFGSLQDIQYVRLVGADAVAFAHAQFANDVQALAIGQWQWNAWLTAKGRVIAIFALLREDDAHLLMLLPD
GNAAEIAAQLGRFVFRRKLKISTAALFAFGGFAAPERARAAQADLGTQRIVLDLGSAALPRTLLLYAEEALAAPIEAPSV
DAQWRRADLQLGLARLVEGQREQWTPQQLALDRLQAYSVKKGCYPGQEIVARTHFLGKAKRALQLLETDSAVEAGDAVAM
DGAAIGTVVSVAGNLALAVLPLELTLDADTPLQAGAHGARPRAIAPGLER
>Mature_289_residues
ADNLNLIPQGFGSLQDIQYVRLVGADAVAFAHAQFANDVQALAIGQWQWNAWLTAKGRVIAIFALLREDDAHLLMLLPDG
NAAEIAAQLGRFVFRRKLKISTAALFAFGGFAAPERARAAQADLGTQRIVLDLGSAALPRTLLLYAEEALAAPIEAPSVD
AQWRRADLQLGLARLVEGQREQWTPQQLALDRLQAYSVKKGCYPGQEIVARTHFLGKAKRALQLLETDSAVEAGDAVAMD
GAAIGTVVSVAGNLALAVLPLELTLDADTPLQAGAHGARPRAIAPGLER

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=240, Percent_Identity=27.9166666666667, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 30968; Mature: 30837

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNLNLIPQGFGSLQDIQYVRLVGADAVAFAHAQFANDVQALAIGQWQWNAWLTAKGRV
CCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHEEEEECEEEEEEEEECCCE
IAIFALLREDDAHLLMLLPDGNAAEIAAQLGRFVFRRKLKISTAALFAFGGFAAPERARA
EEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AQADLGTQRIVLDLGSAALPRTLLLYAEEALAAPIEAPSVDAQWRRADLQLGLARLVEGQ
HHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
REQWTPQQLALDRLQAYSVKKGCYPGQEIVARTHFLGKAKRALQLLETDSAVEAGDAVAM
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
DGAAIGTVVSVAGNLALAVLPLELTLDADTPLQAGAHGARPRAIAPGLER
CCHHHHHHHHHHCCEEEEEEEEEEEECCCCCHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADNLNLIPQGFGSLQDIQYVRLVGADAVAFAHAQFANDVQALAIGQWQWNAWLTAKGRV
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHEEEEECEEEEEEEEECCCE
IAIFALLREDDAHLLMLLPDGNAAEIAAQLGRFVFRRKLKISTAALFAFGGFAAPERARA
EEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AQADLGTQRIVLDLGSAALPRTLLLYAEEALAAPIEAPSVDAQWRRADLQLGLARLVEGQ
HHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
REQWTPQQLALDRLQAYSVKKGCYPGQEIVARTHFLGKAKRALQLLETDSAVEAGDAVAM
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
DGAAIGTVVSVAGNLALAVLPLELTLDADTPLQAGAHGARPRAIAPGLER
CCHHHHHHHHHHCCEEEEEEEEEEEECCCCCHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]