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The map label for this gene is grpE [H]

Identifier: 84623569

GI number: 84623569

Start: 2109565

End: 2110083

Strand: Direct

Name: grpE [H]

Synonym: XOO_1912

Alternate gene names: 84623569

Gene position: 2109565-2110083 (Clockwise)

Preceding gene: 84623568

Following gene: 84623570

Centisome position: 42.7

GC content: 66.09

Gene sequence:

>519_bases
ATGAATCAAGACCACCCCGAATTCGATTCCGAAGACCTGGCCCAGAATCCACCCGAGACCGATCCGCTCAAGGCCGAGAT
CGAATCGCTGCGCAGCGAGATCGCGCTGGTCAAGGCCGATGCCCTGCGCGAGCGCGCCGACCTGGAAAACCAGCGCAAGC
GCATCGCCCGCGACGTGGAGAACGCGCGCAAGTTCGCCAACGAGAAGCTGCTGGGCGAGCTGCTGCCGGTGTTCGACAGC
CTTGACGCGGGCCTGACCGCCGCCGGCACCCAGCCCAGCCCGCTGCGCGATGGTCTGGACATGACCTACAAACAATTGCT
CAAGGTTGCCGCCGACAACGGCCTGACCCTGCTCGACCCGGTCGGCCAGCCGTTCAACCCGGACCAGCACCAGGCGATCA
GCCAGGGCGAGGCCGAGGGTATCGCGCCAGGCCATGTGGTGCAGGTGTTCCAGAAGGGCTACCTGCTCAACGATCGCCTG
CTGCGCCCGGCCCTGGTGGTCGTGGCCAAGCAGGACTGA

Upstream 100 bases:

>100_bases
CCGGCATCGATCGGAACGATCATCATCGCTTGAAACGCGCGCGAGCGCCCATATACGGGGTGACGTTGGGGCGGGCGTTC
CGCTTGCCCGGGAACTGCAC

Downstream 100 bases:

>100_bases
ACCGCAGCGCCGGCTGAACTGTCAGGCGCAGGCATGAAACACAGATGTGCGCCGGTGGCTTGAACACCCGCGCACCACCC
CCACATCTCAGCCAGACCCG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MNQDHPEFDSEDLAQNPPETDPLKAEIESLRSEIALVKADALRERADLENQRKRIARDVENARKFANEKLLGELLPVFDS
LDAGLTAAGTQPSPLRDGLDMTYKQLLKVAADNGLTLLDPVGQPFNPDQHQAISQGEAEGIAPGHVVQVFQKGYLLNDRL
LRPALVVVAKQD

Sequences:

>Translated_172_residues
MNQDHPEFDSEDLAQNPPETDPLKAEIESLRSEIALVKADALRERADLENQRKRIARDVENARKFANEKLLGELLPVFDS
LDAGLTAAGTQPSPLRDGLDMTYKQLLKVAADNGLTLLDPVGQPFNPDQHQAISQGEAEGIAPGHVVQVFQKGYLLNDRL
LRPALVVVAKQD
>Mature_172_residues
MNQDHPEFDSEDLAQNPPETDPLKAEIESLRSEIALVKADALRERADLENQRKRIARDVENARKFANEKLLGELLPVFDS
LDAGLTAAGTQPSPLRDGLDMTYKQLLKVAADNGLTLLDPVGQPFNPDQHQAISQGEAEGIAPGHVVQVFQKGYLLNDRL
LRPALVVVAKQD

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=182, Percent_Identity=34.6153846153846, Blast_Score=80, Evalue=7e-16,
Organism=Homo sapiens, GI40255109, Length=137, Percent_Identity=33.5766423357664, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1788967, Length=173, Percent_Identity=36.9942196531792, Blast_Score=113, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17552458, Length=172, Percent_Identity=33.1395348837209, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=169, Percent_Identity=30.7692307692308, Blast_Score=83, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24653432, Length=182, Percent_Identity=34.0659340659341, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 18927; Mature: 18927

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQDHPEFDSEDLAQNPPETDPLKAEIESLRSEIALVKADALRERADLENQRKRIARDVE
CCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NARKFANEKLLGELLPVFDSLDAGLTAAGTQPSPLRDGLDMTYKQLLKVAADNGLTLLDP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECC
VGQPFNPDQHQAISQGEAEGIAPGHVVQVFQKGYLLNDRLLRPALVVVAKQD
CCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MNQDHPEFDSEDLAQNPPETDPLKAEIESLRSEIALVKADALRERADLENQRKRIARDVE
CCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NARKFANEKLLGELLPVFDSLDAGLTAAGTQPSPLRDGLDMTYKQLLKVAADNGLTLLDP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECC
VGQPFNPDQHQAISQGEAEGIAPGHVVQVFQKGYLLNDRLLRPALVVVAKQD
CCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]