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The map label for this gene is emrB [H]

Identifier: 84623548

GI number: 84623548

Start: 2088741

End: 2090321

Strand: Direct

Name: emrB [H]

Synonym: XOO_1891

Alternate gene names: 84623548

Gene position: 2088741-2090321 (Clockwise)

Preceding gene: 84623547

Following gene: 84623549

Centisome position: 42.28

GC content: 63.38

Gene sequence:

>1581_bases
ATGTCTTCGCAAGCTCCCACCGCGCCCGGCGGCCCGGCGGCGCCGGCGCCCGCGGCGGGTTTTCGACCGGCCAGCGTGGC
GTTGTGCACCGTGGGCCTGGCCATGGCCTCGTTCATGCAGGTGCTGGACACCACCATCGCCAACGTCTCGCTGCCCACCA
TCGCCGGCAACCTCGGCGCCAGTTCGCAGCAGGCCACCTGGGTGATCACTTCGTTCGCGGTCAGCACCGCGATCGCGTTG
CCGTTGACCGGTTGGTTGAGCCGGCGCTTCGGCGAAACCAAATTATTCGTGTGGTCCACGCTGGCATTCACCATTGCCTC
GCTGCTGTGCGGGCTTGCGCAGAGCATGGGCATGCTGGTGGTGGCGCGTGCGTTGCAGGGCGTTGTCGCCGGGCCGATGT
ATCCCATCACCCAGAGTCTGCTGGTGTCGATCTATCCGCGCGAAAAACGCGGGCAGGCGCTGGCGTTGCTGGGGATGATC
ACCGTGGTGGCGCCGATTGCCGGGCCAATTCTCGGTGGCTGGATCACCGACGACTACAGCTGGGAATGGATCTTCTTGAT
CAACGTGCCGCTGGGCATCATCGCCAGCAGTATCGTGGGTTCGCAGTTGCGGCATCGCCCCGAACAGCTCGAAAAGCCGC
GCATGGATTACATCGGCCTGATCCTGCTGGTGGCCGGTGTGGGTGCGCTGCAGTTGGTGCTGGACCTGGGCAATGACGAG
GACTGGTTCTCTTCCGACAAGATCGTGGTGCTGGCCTGCGTCGCTGCGGTGGCGCTGGTGGTGTTCGTGATCTGGGAGCT
GACCGACAAGGACCCCATCGTCGATCTCAAGCTGTTCCGGCATCGCAACTTCCGCACCGGCACGTTGGCGATGGTGGTGG
CGTATGCCGCGTTCTTCAGCGTGAGCCTGCTGATCCCGCAATGGCTGCAGCGCGACATGGGTTACACCGCGATCTGGGCC
GGCCTTGCGACTGCGCCGATCGGCATCCTGCCGGTGCTGATGACGCCGTTCGTGGGCAAATACGCGCTGCGCTTCGACCT
GCGCCTGCTGGCTACCATCGCCTTTATCTTCATGTCATTCACCAGCTTCTTCCGTTCCAACTTCAACCTGCAGGTGGACT
TCAGCCATGTGGCGACCATCCAGCTGGTGATGGGCGTGGGCGTGGCGCTGTTCTTCATGCCGGTGCTGCAGATCCTGCTG
TCGGATCTGGATGGGCGCGAAATCGCTGCAGGTTCTGGTCTGGCCACCTTCCTGCGTACGTTGGGCGGAAGCTTCGCCGC
ATCGTTGAGCACCTACCTGTGGGCACACCGTACCCAGGTGCATCACGCGCACATCACCGAACACATTTCGGTGTACACGC
CCGGCATGCAGGAACGACTGGCTGCGATGGGGCAGGGCGACCTGAAGCGTGGCGCTGCATCGCTCAACAACATGATCAAC
CACCAGGCGTCGCAGATGGGCTTCAACGACATCTTCTATCTGCTTGGTTGGACCTTTCTGGCAATCATCTTCTTCCTGTG
GCTGGCCAAGCGGCCGTTCGGCGCCGGTGCCGGGAGTGCAGCGGCGGCAGCGGGGCACTGA

Upstream 100 bases:

>100_bases
CGTGTATGCGCAGCAGCTCAAGGCTGCCGACGCCGACGTCCAACGCATCATCCAGGACAACCTGCCGTCGCAGGCTGCCA
GCAAGGCGGGCTAAGCCATC

Downstream 100 bases:

>100_bases
GCCCCTGTTGAGGGGCTGCTAGACTTGCGCCGTCAACGCAGGAGCGGGCGATGGGCATGTGGTCGGCAGCGATGGTATGC
AGGCAAATGGATCTGCTCGA

Product: multidrug resistance membrane translocase

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSSQAPTAPGGPAAPAPAAGFRPASVALCTVGLAMASFMQVLDTTIANVSLPTIAGNLGASSQQATWVITSFAVSTAIAL
PLTGWLSRRFGETKLFVWSTLAFTIASLLCGLAQSMGMLVVARALQGVVAGPMYPITQSLLVSIYPREKRGQALALLGMI
TVVAPIAGPILGGWITDDYSWEWIFLINVPLGIIASSIVGSQLRHRPEQLEKPRMDYIGLILLVAGVGALQLVLDLGNDE
DWFSSDKIVVLACVAAVALVVFVIWELTDKDPIVDLKLFRHRNFRTGTLAMVVAYAAFFSVSLLIPQWLQRDMGYTAIWA
GLATAPIGILPVLMTPFVGKYALRFDLRLLATIAFIFMSFTSFFRSNFNLQVDFSHVATIQLVMGVGVALFFMPVLQILL
SDLDGREIAAGSGLATFLRTLGGSFAASLSTYLWAHRTQVHHAHITEHISVYTPGMQERLAAMGQGDLKRGAASLNNMIN
HQASQMGFNDIFYLLGWTFLAIIFFLWLAKRPFGAGAGSAAAAAGH

Sequences:

>Translated_526_residues
MSSQAPTAPGGPAAPAPAAGFRPASVALCTVGLAMASFMQVLDTTIANVSLPTIAGNLGASSQQATWVITSFAVSTAIAL
PLTGWLSRRFGETKLFVWSTLAFTIASLLCGLAQSMGMLVVARALQGVVAGPMYPITQSLLVSIYPREKRGQALALLGMI
TVVAPIAGPILGGWITDDYSWEWIFLINVPLGIIASSIVGSQLRHRPEQLEKPRMDYIGLILLVAGVGALQLVLDLGNDE
DWFSSDKIVVLACVAAVALVVFVIWELTDKDPIVDLKLFRHRNFRTGTLAMVVAYAAFFSVSLLIPQWLQRDMGYTAIWA
GLATAPIGILPVLMTPFVGKYALRFDLRLLATIAFIFMSFTSFFRSNFNLQVDFSHVATIQLVMGVGVALFFMPVLQILL
SDLDGREIAAGSGLATFLRTLGGSFAASLSTYLWAHRTQVHHAHITEHISVYTPGMQERLAAMGQGDLKRGAASLNNMIN
HQASQMGFNDIFYLLGWTFLAIIFFLWLAKRPFGAGAGSAAAAAGH
>Mature_525_residues
SSQAPTAPGGPAAPAPAAGFRPASVALCTVGLAMASFMQVLDTTIANVSLPTIAGNLGASSQQATWVITSFAVSTAIALP
LTGWLSRRFGETKLFVWSTLAFTIASLLCGLAQSMGMLVVARALQGVVAGPMYPITQSLLVSIYPREKRGQALALLGMIT
VVAPIAGPILGGWITDDYSWEWIFLINVPLGIIASSIVGSQLRHRPEQLEKPRMDYIGLILLVAGVGALQLVLDLGNDED
WFSSDKIVVLACVAAVALVVFVIWELTDKDPIVDLKLFRHRNFRTGTLAMVVAYAAFFSVSLLIPQWLQRDMGYTAIWAG
LATAPIGILPVLMTPFVGKYALRFDLRLLATIAFIFMSFTSFFRSNFNLQVDFSHVATIQLVMGVGVALFFMPVLQILLS
DLDGREIAAGSGLATFLRTLGGSFAASLSTYLWAHRTQVHHAHITEHISVYTPGMQERLAAMGQGDLKRGAASLNNMINH
QASQMGFNDIFYLLGWTFLAIIFFLWLAKRPFGAGAGSAAAAAGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=494, Percent_Identity=47.165991902834, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1788710, Length=506, Percent_Identity=43.2806324110672, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1790195, Length=473, Percent_Identity=29.3868921775898, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1788392, Length=316, Percent_Identity=34.1772151898734, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87081983, Length=413, Percent_Identity=27.8450363196126, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790146, Length=172, Percent_Identity=27.3255813953488, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788509, Length=136, Percent_Identity=27.2058823529412, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1789152, Length=186, Percent_Identity=25.2688172043011, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=280, Percent_Identity=25.7142857142857, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322958, Length=306, Percent_Identity=23.8562091503268, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319770, Length=328, Percent_Identity=26.5243902439024, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=26.797385620915, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323520, Length=371, Percent_Identity=22.911051212938, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56527; Mature: 56396

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQAPTAPGGPAAPAPAAGFRPASVALCTVGLAMASFMQVLDTTIANVSLPTIAGNLGA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCC
SSQQATWVITSFAVSTAIALPLTGWLSRRFGETKLFVWSTLAFTIASLLCGLAQSMGMLV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VARALQGVVAGPMYPITQSLLVSIYPREKRGQALALLGMITVVAPIAGPILGGWITDDYS
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCC
WEWIFLINVPLGIIASSIVGSQLRHRPEQLEKPRMDYIGLILLVAGVGALQLVLDLGNDE
EEEEEEEECCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DWFSSDKIVVLACVAAVALVVFVIWELTDKDPIVDLKLFRHRNFRTGTLAMVVAYAAFFS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHH
VSLLIPQWLQRDMGYTAIWAGLATAPIGILPVLMTPFVGKYALRFDLRLLATIAFIFMSF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSFFRSNFNLQVDFSHVATIQLVMGVGVALFFMPVLQILLSDLDGREIAAGSGLATFLRT
HHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LGGSFAASLSTYLWAHRTQVHHAHITEHISVYTPGMQERLAAMGQGDLKRGAASLNNMIN
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCHHHHHHHHHHHHHH
HQASQMGFNDIFYLLGWTFLAIIFFLWLAKRPFGAGAGSAAAAAGH
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSQAPTAPGGPAAPAPAAGFRPASVALCTVGLAMASFMQVLDTTIANVSLPTIAGNLGA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCC
SSQQATWVITSFAVSTAIALPLTGWLSRRFGETKLFVWSTLAFTIASLLCGLAQSMGMLV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VARALQGVVAGPMYPITQSLLVSIYPREKRGQALALLGMITVVAPIAGPILGGWITDDYS
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCC
WEWIFLINVPLGIIASSIVGSQLRHRPEQLEKPRMDYIGLILLVAGVGALQLVLDLGNDE
EEEEEEEECCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DWFSSDKIVVLACVAAVALVVFVIWELTDKDPIVDLKLFRHRNFRTGTLAMVVAYAAFFS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHH
VSLLIPQWLQRDMGYTAIWAGLATAPIGILPVLMTPFVGKYALRFDLRLLATIAFIFMSF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSFFRSNFNLQVDFSHVATIQLVMGVGVALFFMPVLQILLSDLDGREIAAGSGLATFLRT
HHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LGGSFAASLSTYLWAHRTQVHHAHITEHISVYTPGMQERLAAMGQGDLKRGAASLNNMIN
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCHHHHHHHHHHHHHH
HQASQMGFNDIFYLLGWTFLAIIFFLWLAKRPFGAGAGSAAAAAGH
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]