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The map label for this gene is yngF [H]

Identifier: 84623398

GI number: 84623398

Start: 1923067

End: 1924239

Strand: Direct

Name: yngF [H]

Synonym: XOO_1741

Alternate gene names: 84623398

Gene position: 1923067-1924239 (Clockwise)

Preceding gene: 84623397

Following gene: 84623399

Centisome position: 38.93

GC content: 65.47

Gene sequence:

>1173_bases
ATGGCGCACGTCAGCGCAACCGGCGACGCACCGGTGCTGTTCGAGCAGCGCACCTGTGCCGATGGACATCGCATCGGTAT
CGCCACCTTGAATGCGCCCAAGACGCTCAATGGCCTGTCGTTGCAGATGACGCGGCTACTGGATGCGCAATTGCGCGCTT
GGGCCGAGGATGCGCAGATCGCGTGTGTGGTGTTGCGCGGCGCCGGTGAAAAGGCGTTCTGCGCTGGCGGCGATCTGCAT
GGCCTGTACCAGAGCATGCATGCGCATCGCGATGCGGTGCCCGACAGTGCAGCGCGCCGCGCAGCGCCGCAGGCCAATGC
GCATGCCGCGGCGTTCTTCGAAGAGGAATACCGGCTCGACCATCGCATCCACACGTATCCGAAGCCCCTGCTGTGCTGGG
GCCACGGCATCGTGATGGGCGGTGGCATCGGCTTGATGTCCGGTGCCAGCCATCGTGTGGTCACCGAGCGCTCGCGCCTG
GCCATGCCGGAAATCAGCGTCGGCTTGTTCCCGGATGTGGGGGGAAGTTGGTTGTTGCGCCGTGTGCCGCGGGGCGCCGG
CCTGTTTCTGGCGCTGACTGGCGCGCCGCTGGATACCAGCGATGCGGTTTATGCAGGGCTCGCCGACGTGCGCCTGGAGC
ATGCGCAGTACGCAGCGGTGCTGGATGCGCTAAGTGCGCACGCCTGGGCAGGCAATGCCGACGACGACCGCACCCAACTC
AGTGCATTCCTGCATGGCATCGCGCAACCCACCGAGCCGGGCCCGCTACAAATCCACGCACCGTTGATCGAACAGTGGGT
GGCTGGCGAATCGCTGGAACACGTTGTCGCTGCAATTCTGGCGCTGCAAAGCGAAGACAGCTGGTTGCAGGCTGCACATG
CCACCTTGGCCGCGGGTGCTCCGGGGTCTGCGCGCCTGGCCTGGGAGCTGCATCGCCATGCGGGCACCACGACGCTGGCC
GACACCTTCCGTACCGAATACGTGGTCGCATTGCATGCGGCGGCACACGGCGATTTTGCCGAAGGCATCCGCGCGCTGTT
GATCGACAAGGATCGTCAACCGCACTGGCAACCGGCGTCGCTGGAAGCAGCCGATGCGCAGTGGGCCGCCGATTTCTTCG
TTGTGCCCTGGTCGGCTGCACAGCATCCGCTTGCCGATCTGGGCAGCAGTTGA

Upstream 100 bases:

>100_bases
CCGAGCGCGAAGCATTTGTGGATCTATTCGAACACGTCGATCAGGCCGAAGGCGTGGCCGCATTTCTGGAGAAGCGCGCG
CCGCAGTGGAGTAACCACTG

Downstream 100 bases:

>100_bases
GGCCGGTGCGCGCCGAGTGGCTGGCAATGTGATGGGCACTGCAAGTGCGTATAAGCGCGGCGCTCGCTCTGTCCGAACAC
TGCAGTCGCACTGGAAGCTG

Product: enoyl-CoA hydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MAHVSATGDAPVLFEQRTCADGHRIGIATLNAPKTLNGLSLQMTRLLDAQLRAWAEDAQIACVVLRGAGEKAFCAGGDLH
GLYQSMHAHRDAVPDSAARRAAPQANAHAAAFFEEEYRLDHRIHTYPKPLLCWGHGIVMGGGIGLMSGASHRVVTERSRL
AMPEISVGLFPDVGGSWLLRRVPRGAGLFLALTGAPLDTSDAVYAGLADVRLEHAQYAAVLDALSAHAWAGNADDDRTQL
SAFLHGIAQPTEPGPLQIHAPLIEQWVAGESLEHVVAAILALQSEDSWLQAAHATLAAGAPGSARLAWELHRHAGTTTLA
DTFRTEYVVALHAAAHGDFAEGIRALLIDKDRQPHWQPASLEAADAQWAADFFVVPWSAAQHPLADLGSS

Sequences:

>Translated_390_residues
MAHVSATGDAPVLFEQRTCADGHRIGIATLNAPKTLNGLSLQMTRLLDAQLRAWAEDAQIACVVLRGAGEKAFCAGGDLH
GLYQSMHAHRDAVPDSAARRAAPQANAHAAAFFEEEYRLDHRIHTYPKPLLCWGHGIVMGGGIGLMSGASHRVVTERSRL
AMPEISVGLFPDVGGSWLLRRVPRGAGLFLALTGAPLDTSDAVYAGLADVRLEHAQYAAVLDALSAHAWAGNADDDRTQL
SAFLHGIAQPTEPGPLQIHAPLIEQWVAGESLEHVVAAILALQSEDSWLQAAHATLAAGAPGSARLAWELHRHAGTTTLA
DTFRTEYVVALHAAAHGDFAEGIRALLIDKDRQPHWQPASLEAADAQWAADFFVVPWSAAQHPLADLGSS
>Mature_389_residues
AHVSATGDAPVLFEQRTCADGHRIGIATLNAPKTLNGLSLQMTRLLDAQLRAWAEDAQIACVVLRGAGEKAFCAGGDLHG
LYQSMHAHRDAVPDSAARRAAPQANAHAAAFFEEEYRLDHRIHTYPKPLLCWGHGIVMGGGIGLMSGASHRVVTERSRLA
MPEISVGLFPDVGGSWLLRRVPRGAGLFLALTGAPLDTSDAVYAGLADVRLEHAQYAAVLDALSAHAWAGNADDDRTQLS
AFLHGIAQPTEPGPLQIHAPLIEQWVAGESLEHVVAAILALQSEDSWLQAAHATLAAGAPGSARLAWELHRHAGTTTLAD
TFRTEYVVALHAAAHGDFAEGIRALLIDKDRQPHWQPASLEAADAQWAADFFVVPWSAAQHPLADLGSS

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=362, Percent_Identity=31.2154696132597, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI37594469, Length=316, Percent_Identity=30.379746835443, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1787659, Length=200, Percent_Identity=28, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1787660, Length=175, Percent_Identity=32.5714285714286, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=350, Percent_Identity=35.4285714285714, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI25144157, Length=350, Percent_Identity=35.4285714285714, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320241, Length=385, Percent_Identity=27.7922077922078, Blast_Score=132, Evalue=6e-32,
Organism=Drosophila melanogaster, GI28571729, Length=349, Percent_Identity=33.810888252149, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI28571730, Length=349, Percent_Identity=33.810888252149, Blast_Score=190, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 41543; Mature: 41412

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHVSATGDAPVLFEQRTCADGHRIGIATLNAPKTLNGLSLQMTRLLDAQLRAWAEDAQI
CCEECCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEE
ACVVLRGAGEKAFCAGGDLHGLYQSMHAHRDAVPDSAARRAAPQANAHAAAFFEEEYRLD
EEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHC
HRIHTYPKPLLCWGHGIVMGGGIGLMSGASHRVVTERSRLAMPEISVGLFPDVGGSWLLR
CHHCCCCCCEEEECCCEEEECCCCCCCCCCCCEEEHHHHCCCCCCCEECCCCCCHHHHHH
RVPRGAGLFLALTGAPLDTSDAVYAGLADVRLEHAQYAAVLDALSAHAWAGNADDDRTQL
HCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
SAFLHGIAQPTEPGPLQIHAPLIEQWVAGESLEHVVAAILALQSEDSWLQAAHATLAAGA
HHHHHHHCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
PGSARLAWELHRHAGTTTLADTFRTEYVVALHAAAHGDFAEGIRALLIDKDRQPHWQPAS
CCCCEEEEEEHHHCCCHHHHHHHHHEEEEEEEEHHCCCHHHHHHEEEEECCCCCCCCCCC
LEAADAQWAADFFVVPWSAAQHPLADLGSS
CCCCCCCCCCCEEEECCCCCCCCHHHCCCC
>Mature Secondary Structure 
AHVSATGDAPVLFEQRTCADGHRIGIATLNAPKTLNGLSLQMTRLLDAQLRAWAEDAQI
CEECCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEE
ACVVLRGAGEKAFCAGGDLHGLYQSMHAHRDAVPDSAARRAAPQANAHAAAFFEEEYRLD
EEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHC
HRIHTYPKPLLCWGHGIVMGGGIGLMSGASHRVVTERSRLAMPEISVGLFPDVGGSWLLR
CHHCCCCCCEEEECCCEEEECCCCCCCCCCCCEEEHHHHCCCCCCCEECCCCCCHHHHHH
RVPRGAGLFLALTGAPLDTSDAVYAGLADVRLEHAQYAAVLDALSAHAWAGNADDDRTQL
HCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
SAFLHGIAQPTEPGPLQIHAPLIEQWVAGESLEHVVAAILALQSEDSWLQAAHATLAAGA
HHHHHHHCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
PGSARLAWELHRHAGTTTLADTFRTEYVVALHAAAHGDFAEGIRALLIDKDRQPHWQPAS
CCCCEEEEEEHHHCCCHHHHHHHHHEEEEEEEEHHCCCHHHHHHEEEEECCCCCCCCCCC
LEAADAQWAADFFVVPWSAAQHPLADLGSS
CCCCCCCCCCCEEEECCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]