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The map label for this gene is paaF [C]

Identifier: 84623397

GI number: 84623397

Start: 1922270

End: 1923067

Strand: Direct

Name: paaF [C]

Synonym: XOO_1740

Alternate gene names: 84623397

Gene position: 1922270-1923067 (Clockwise)

Preceding gene: 84623396

Following gene: 84623398

Centisome position: 38.91

GC content: 63.91

Gene sequence:

>798_bases
ATGAGCGATTGGGAACAGGGCGTCCATACCGGTCTGCAGGTCGAGCGCGATGGTCACGTGGCCATCGTGACCTTGAGCAA
CCCACCGGCCAACACCTGGACCGTGCACAGCCTGGCGGCTCTGCGCGATTTGGTGCGCGCACTGGATGCAGATCGCAGCA
TCTACGCCTTGGTGATCAGCGGCGAGGGCGAGAAATTCTTCAGCGCCGGTGCCGATCTCAAGCAGTTCGCCGATGGCGAC
AAGGCCAACGCACGCGAGGCCGCGCGCCGGTTTGGCGAAGCTTTCGAAGCCTTGAGTGCGTTTCGCGGCGTGTCGATCGC
TGCAATCAACGGCTATGCGATGGGGGGCGGGCTGGAGTGCGCATTGGCCTGCGATCTACGCATCGCCGAGCAGCAAGCAC
ATCTTGCGTTGCCCGAAGCCAGTGTTGGTCTGCTGCCATGTGCAGGCGGTACCCAGAATCTGCCGCGCGTGGTCGGCGAA
GGTTGGGCCAAGCGCATGATCCTGCTCGGTGAGCGTGTTGATGCTGCCACCGCGCAGCGCATCGGGTTGGTGGAAGAGGT
GGTTGGCAAGGGCGAGTCGCGCGCCTTGGCGATTGCCTGGGCGCAGCGCGCCGGTAAACAGAGCCCAATCAGCGTGTCGG
CGTGCAAGCAGCTGGTGCAGTCCACGCGCCATGGCACGCACGCAGCCGCGCTGATTGCCGAGCGCGAAGCATTTGTGGAT
CTATTCGAACACGTCGATCAGGCCGAAGGCGTGGCCGCATTTCTGGAGAAGCGCGCGCCGCAGTGGAGTAACCACTGA

Upstream 100 bases:

>100_bases
TGCTGCGCGACAGCCGCGTGCACCGCATTCTGGAAGGTACCAACGAGGTGATGCGCATGATCGTGGCGCGTCATCTGCTC
AATGGCGAGGAGGAACTGCG

Downstream 100 bases:

>100_bases
TGGCGCACGTCAGCGCAACCGGCGACGCACCGGTGCTGTTCGAGCAGCGCACCTGTGCCGATGGACATCGCATCGGTATC
GCCACCTTGAATGCGCCCAA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSDWEQGVHTGLQVERDGHVAIVTLSNPPANTWTVHSLAALRDLVRALDADRSIYALVISGEGEKFFSAGADLKQFADGD
KANAREAARRFGEAFEALSAFRGVSIAAINGYAMGGGLECALACDLRIAEQQAHLALPEASVGLLPCAGGTQNLPRVVGE
GWAKRMILLGERVDAATAQRIGLVEEVVGKGESRALAIAWAQRAGKQSPISVSACKQLVQSTRHGTHAAALIAEREAFVD
LFEHVDQAEGVAAFLEKRAPQWSNH

Sequences:

>Translated_265_residues
MSDWEQGVHTGLQVERDGHVAIVTLSNPPANTWTVHSLAALRDLVRALDADRSIYALVISGEGEKFFSAGADLKQFADGD
KANAREAARRFGEAFEALSAFRGVSIAAINGYAMGGGLECALACDLRIAEQQAHLALPEASVGLLPCAGGTQNLPRVVGE
GWAKRMILLGERVDAATAQRIGLVEEVVGKGESRALAIAWAQRAGKQSPISVSACKQLVQSTRHGTHAAALIAEREAFVD
LFEHVDQAEGVAAFLEKRAPQWSNH
>Mature_264_residues
SDWEQGVHTGLQVERDGHVAIVTLSNPPANTWTVHSLAALRDLVRALDADRSIYALVISGEGEKFFSAGADLKQFADGDK
ANAREAARRFGEAFEALSAFRGVSIAAINGYAMGGGLECALACDLRIAEQQAHLALPEASVGLLPCAGGTQNLPRVVGEG
WAKRMILLGERVDAATAQRIGLVEEVVGKGESRALAIAWAQRAGKQSPISVSACKQLVQSTRHGTHAAALIAEREAFVDL
FEHVDQAEGVAAFLEKRAPQWSNH

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=31.8725099601594, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI68989263, Length=202, Percent_Identity=36.6336633663366, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI4502327, Length=239, Percent_Identity=29.2887029288703, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI37594471, Length=239, Percent_Identity=26.7782426778243, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI37594469, Length=239, Percent_Identity=26.7782426778243, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI20127408, Length=179, Percent_Identity=33.5195530726257, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=29.3650793650794, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI261878539, Length=109, Percent_Identity=43.1192660550459, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=27.3913043478261, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=27.3913043478261, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI150378541, Length=251, Percent_Identity=27.8884462151394, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI62530384, Length=244, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI70995211, Length=256, Percent_Identity=28.125, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI157694520, Length=184, Percent_Identity=27.1739130434783, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI295842266, Length=242, Percent_Identity=24.3801652892562, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=254, Percent_Identity=38.5826771653543, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI221142681, Length=255, Percent_Identity=33.7254901960784, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788682, Length=178, Percent_Identity=35.9550561797753, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790281, Length=287, Percent_Identity=27.5261324041812, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082183, Length=235, Percent_Identity=24.6808510638298, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787660, Length=249, Percent_Identity=29.718875502008, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=25.2, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=255, Percent_Identity=34.1176470588235, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=34.8178137651822, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17540714, Length=233, Percent_Identity=29.1845493562232, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17558304, Length=186, Percent_Identity=33.8709677419355, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25144276, Length=186, Percent_Identity=36.5591397849462, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=186, Percent_Identity=36.5591397849462, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=186, Percent_Identity=36.5591397849462, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=262, Percent_Identity=28.6259541984733, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=203, Percent_Identity=27.5862068965517, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=230, Percent_Identity=28.2608695652174, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=211, Percent_Identity=27.9620853080569, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=257, Percent_Identity=25.6809338521401, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=241, Percent_Identity=25.7261410788382, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=190, Percent_Identity=27.3684210526316, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=190, Percent_Identity=27.3684210526316, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI20129971, Length=266, Percent_Identity=32.7067669172932, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24653477, Length=266, Percent_Identity=32.7067669172932, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24653139, Length=268, Percent_Identity=29.8507462686567, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24650670, Length=190, Percent_Identity=30.5263157894737, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922422, Length=246, Percent_Identity=23.9837398373984, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24654903, Length=251, Percent_Identity=23.1075697211155, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357171, Length=239, Percent_Identity=28.0334728033473, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550169, Length=254, Percent_Identity=24.4094488188976, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24583165, Length=209, Percent_Identity=23.9234449760766, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19921018, Length=187, Percent_Identity=25.668449197861, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28201; Mature: 28070

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDWEQGVHTGLQVERDGHVAIVTLSNPPANTWTVHSLAALRDLVRALDADRSIYALVIS
CCCHHHHHHCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEE
GEGEKFFSAGADLKQFADGDKANAREAARRFGEAFEALSAFRGVSIAAINGYAMGGGLEC
CCCCHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCEE
ALACDLRIAEQQAHLALPEASVGLLPCAGGTQNLPRVVGEGWAKRMILLGERVDAATAQR
EEEECCHHHHHHHHEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IGLVEEVVGKGESRALAIAWAQRAGKQSPISVSACKQLVQSTRHGTHAAALIAEREAFVD
HHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LFEHVDQAEGVAAFLEKRAPQWSNH
HHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDWEQGVHTGLQVERDGHVAIVTLSNPPANTWTVHSLAALRDLVRALDADRSIYALVIS
CCHHHHHHCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEE
GEGEKFFSAGADLKQFADGDKANAREAARRFGEAFEALSAFRGVSIAAINGYAMGGGLEC
CCCCHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCEE
ALACDLRIAEQQAHLALPEASVGLLPCAGGTQNLPRVVGEGWAKRMILLGERVDAATAQR
EEEECCHHHHHHHHEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IGLVEEVVGKGESRALAIAWAQRAGKQSPISVSACKQLVQSTRHGTHAAALIAEREAFVD
HHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LFEHVDQAEGVAAFLEKRAPQWSNH
HHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA