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The map label for this gene is metL [H]

Identifier: 84623375

GI number: 84623375

Start: 1893384

End: 1894058

Strand: Direct

Name: metL [H]

Synonym: XOO_1718

Alternate gene names: 84623375

Gene position: 1893384-1894058 (Clockwise)

Preceding gene: 84623374

Following gene: 84623377

Centisome position: 38.33

GC content: 65.48

Gene sequence:

>675_bases
ATGCTGCGTGCTGCGTGTCATGCCAGCGGCGCGCATTACGGCGACAGCGCCACCGTGGGCGCTGGGTTGCCGGTGTTGAG
CAGCGTGCGTGCGTTGGTCGCCGGCGGCGATCACATTCATTCGATCAAAGGCGTGTTGTCCGGATCGTTGGCGTGGCTGT
TTCATCGCTACGACGGCAGTGGTGCGTTTTCCGATTGCGTGCGCGAAGCAATAGCGGCTGGTTATACCGAGCCGGATCCG
CGCATCGATTTGTCGGGCGAAGATGTACGGCGCAAGCTGCTGATCCTGGCGCGTGCCGCAGGTTGGCAGCTGGAAGCTGC
GCAGGTGCATGTGGAATCGCTCGTGCCGGCCGTTGTCGCCAAGCTGCCGCTTGCCGAGTTGGATGCGCATTTGCGCGCGC
TGGATGCAGTGGTGGGTGCGCGTTGGCAACAGGCGCGCGCGGCAGGGCGTTGCCTGCGGTTCGTTGGACGTGTGGACGCA
CATGGGGCGAGCGTCGGCTTGCGCGAGTTGGCGCCGGATCATCCCTTGGCCGGCGGTGCTGGTACCGACAATCGGGTTGC
GATCAGCAGCGACCGCTATCGTGCGCAGCCGTTATTGATCCAGGGGCCGGGCGCCGGTGCCGAAGTGACTGCAGCGGCGC
TGCTGGACGATGTGTTGCGGATCGTGGCCGGGTGA

Upstream 100 bases:

>100_bases
GTCGCCGATTGGCATGTCCAATGGTTGCTGCGCGGTGTGCATGTGGTCACCGCCAACAAACTGGGCCAGGGCACCGCGTT
GTCGCGTGCGCAGGCGCTGC

Downstream 100 bases:

>100_bases
TGCGCTGAACGATGAGCGCTTGAGCGTTTCAGACAGCCGCGCTCGTCGCTTGGGCAGGCGACTTATGGACGCTCGTATTG
CTGCGCGATGGTACTGGATG

Product: putative homoserine dehydrogenase

Products: NA

Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MLRAACHASGAHYGDSATVGAGLPVLSSVRALVAGGDHIHSIKGVLSGSLAWLFHRYDGSGAFSDCVREAIAAGYTEPDP
RIDLSGEDVRRKLLILARAAGWQLEAAQVHVESLVPAVVAKLPLAELDAHLRALDAVVGARWQQARAAGRCLRFVGRVDA
HGASVGLRELAPDHPLAGGAGTDNRVAISSDRYRAQPLLIQGPGAGAEVTAAALLDDVLRIVAG

Sequences:

>Translated_224_residues
MLRAACHASGAHYGDSATVGAGLPVLSSVRALVAGGDHIHSIKGVLSGSLAWLFHRYDGSGAFSDCVREAIAAGYTEPDP
RIDLSGEDVRRKLLILARAAGWQLEAAQVHVESLVPAVVAKLPLAELDAHLRALDAVVGARWQQARAAGRCLRFVGRVDA
HGASVGLRELAPDHPLAGGAGTDNRVAISSDRYRAQPLLIQGPGAGAEVTAAALLDDVLRIVAG
>Mature_224_residues
MLRAACHASGAHYGDSATVGAGLPVLSSVRALVAGGDHIHSIKGVLSGSLAWLFHRYDGSGAFSDCVREAIAAGYTEPDP
RIDLSGEDVRRKLLILARAAGWQLEAAQVHVESLVPAVVAKLPLAELDAHLRALDAVVGARWQQARAAGRCLRFVGRVDA
HGASVGLRELAPDHPLAGGAGTDNRVAISSDRYRAQPLLIQGPGAGAEVTAAALLDDVLRIVAG

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0460

COG function: function code E; Homoserine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790376, Length=218, Percent_Identity=48.1651376146789, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1786183, Length=224, Percent_Identity=41.0714285714286, Blast_Score=159, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322599, Length=213, Percent_Identity=40.8450704225352, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 23221; Mature: 23221

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS01042 HOMOSER_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRAACHASGAHYGDSATVGAGLPVLSSVRALVAGGDHIHSIKGVLSGSLAWLFHRYDGS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEECCC
GAFSDCVREAIAAGYTEPDPRIDLSGEDVRRKLLILARAAGWQLEAAQVHVESLVPAVVA
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHH
KLPLAELDAHLRALDAVVGARWQQARAAGRCLRFVGRVDAHGASVGLRELAPDHPLAGGA
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCC
GTDNRVAISSDRYRAQPLLIQGPGAGAEVTAAALLDDVLRIVAG
CCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLRAACHASGAHYGDSATVGAGLPVLSSVRALVAGGDHIHSIKGVLSGSLAWLFHRYDGS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEECCC
GAFSDCVREAIAAGYTEPDPRIDLSGEDVRRKLLILARAAGWQLEAAQVHVESLVPAVVA
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHH
KLPLAELDAHLRALDAVVGARWQQARAAGRCLRFVGRVDAHGASVGLRELAPDHPLAGGA
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCC
GTDNRVAISSDRYRAQPLLIQGPGAGAEVTAAALLDDVLRIVAG
CCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6298218; 8346018; 9278503 [H]