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The map label for this gene is metB [H]

Identifier: 84623374

GI number: 84623374

Start: 1891749

End: 1892966

Strand: Direct

Name: metB [H]

Synonym: XOO_1717

Alternate gene names: 84623374

Gene position: 1891749-1892966 (Clockwise)

Preceding gene: 84623373

Following gene: 84623375

Centisome position: 38.29

GC content: 64.12

Gene sequence:

>1218_bases
ATGAGCTTTCGTGACCCCACCGACACCCCCTGTACTGCTGCCACTGCCGCCGTGCGTGCCGGTATCGACCGCGATACCGC
CTACGGCGCGGTGACCCCGCCCATCGTGCTGTCATCGAATTTTTCGTTCGATGGTTTCGGCAACAAGCGCCAGTACGACT
ACACCCGCAGCGGCAACCCGACCCGCGACCTGCTTGGCGAAGCGCTGGCCGAACTAGAAGGCGGCGCAGGTGGGGTGATT
ACCTCCACCGGCATGGGCGCGATCAATCTGGTGCTCAACGCGGTGCTGCAGCCGGGCGATACGCTGGTGGTGCCGCACGA
TGCGTACGGCGGCAGCTGGCGTCTGTTCAATGCGCTGGCCAAGAAAGGCCACTTTGCGCTGATCACCGCCGATCTCACCG
ACCCGCGTTCGCTGGCCGATGCCTTGGCGCAGTCGCCCAAGTTGGTGCTGATCGAAACCCCGTCCAACCCGCTGCTGCGC
ATCACCGATCTGCGCTTCGTCATCGAAGCTGCGAAGAAGGTCGGTGCATTGACGGTGGTGGACAACACCTTCCTGTCGCC
CGCGCTGCAGAAGCCGTTGGATTTCGGCGCCGATCTGGTGCTGCATTCCACCACCAAGTACATCAATGGCCATAGCGATG
TGGTGGGCGGTGCGGTGGTAGCGCGCGATGCGGAGCTGCATCAGCAACTGGTGTGGTGGGCCAATGCGCTGGGCCTGACC
GGCTCGCCGTTCGATGCCTTCCTCACCTTGCGTGGGCTGCGCACGCTGGACGCACGCCTGCGCGTGCATCAGGAAAATGC
CGATGCGATCGCCGAGTTGCTGGATGGGCATGCGATGGTGAATCAGGTGTATTTCCCCGGTCTGGCGACGCACCCGGGTC
ATGCGCTGGCTGCGCGCCAGCAGAAGGGCTTCGGCGCGATGATGAGCTTCGAGCTGGAAGGCGGCGAAGCGGCAGTGCGC
GCGTTCGTCGATGGCCTGCGCTACTTCACCCTGGCCGAATCGCTGGGCGGGGTGGAAAGCCTGATTGCCCATCCGGCTTC
GATGACGCATGCGGCGATGACTGCCGAAGCGCGTGCGGCAGCCGGCATTTCCGATGGTCTGCTGCGGCTGTCGATCGGGA
TCGAGTCGGCCGAAGATCTGCTGATCGATCTGCGTGCCGGCCTGTCCCGCGCAGAAGCCACCTTGACCACCACCAACCGT
AAAAAAGTCGACGCGTGA

Upstream 100 bases:

>100_bases
GCGTGTTGCGCTCGCCTTATGGCCACGACGCCTTCCTCAAAGAAATCGATCGTATCGATGCAATCCTCACTACCGCCCTT
CGCAGCACCGGAGAAACCAC

Downstream 100 bases:

>100_bases
GCACGGTGCTGCCTGCTTCGCGCCGCGCGCCAGTCGCTGCGGTGACGCCGACGCCGCGGCTGGCCTTGCTGGGCACAGGC
ACCGTGGGCCGTGCTTTTGT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSFRDPTDTPCTAATAAVRAGIDRDTAYGAVTPPIVLSSNFSFDGFGNKRQYDYTRSGNPTRDLLGEALAELEGGAGGVI
TSTGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALAKKGHFALITADLTDPRSLADALAQSPKLVLIETPSNPLLR
ITDLRFVIEAAKKVGALTVVDNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAELHQQLVWWANALGLT
GSPFDAFLTLRGLRTLDARLRVHQENADAIAELLDGHAMVNQVYFPGLATHPGHALAARQQKGFGAMMSFELEGGEAAVR
AFVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAAAGISDGLLRLSIGIESAEDLLIDLRAGLSRAEATLTTTNR
KKVDA

Sequences:

>Translated_405_residues
MSFRDPTDTPCTAATAAVRAGIDRDTAYGAVTPPIVLSSNFSFDGFGNKRQYDYTRSGNPTRDLLGEALAELEGGAGGVI
TSTGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALAKKGHFALITADLTDPRSLADALAQSPKLVLIETPSNPLLR
ITDLRFVIEAAKKVGALTVVDNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAELHQQLVWWANALGLT
GSPFDAFLTLRGLRTLDARLRVHQENADAIAELLDGHAMVNQVYFPGLATHPGHALAARQQKGFGAMMSFELEGGEAAVR
AFVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAAAGISDGLLRLSIGIESAEDLLIDLRAGLSRAEATLTTTNR
KKVDA
>Mature_404_residues
SFRDPTDTPCTAATAAVRAGIDRDTAYGAVTPPIVLSSNFSFDGFGNKRQYDYTRSGNPTRDLLGEALAELEGGAGGVIT
STGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALAKKGHFALITADLTDPRSLADALAQSPKLVLIETPSNPLLRI
TDLRFVIEAAKKVGALTVVDNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAELHQQLVWWANALGLTG
SPFDAFLTLRGLRTLDARLRVHQENADAIAELLDGHAMVNQVYFPGLATHPGHALAARQQKGFGAMMSFELEGGEAAVRA
FVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAAAGISDGLLRLSIGIESAEDLLIDLRAGLSRAEATLTTTNRK
KVDA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=362, Percent_Identity=40.6077348066298, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI299473759, Length=362, Percent_Identity=38.3977900552486, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI25453487, Length=361, Percent_Identity=36.5650969529086, Blast_Score=217, Evalue=1e-56,
Organism=Escherichia coli, GI1790375, Length=378, Percent_Identity=59.2592592592593, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=32.824427480916, Blast_Score=165, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17538055, Length=365, Percent_Identity=44.9315068493151, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17560056, Length=348, Percent_Identity=45.1149425287356, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=35.4054054054054, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6319307, Length=388, Percent_Identity=43.5567010309278, Blast_Score=279, Evalue=7e-76,
Organism=Saccharomyces cerevisiae, GI6321254, Length=411, Percent_Identity=32.360097323601, Blast_Score=196, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6321904, Length=398, Percent_Identity=29.1457286432161, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=27.4193548387097, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321170, Length=325, Percent_Identity=29.5384615384615, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323557, Length=251, Percent_Identity=26.2948207171315, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322970, Length=274, Percent_Identity=25.9124087591241, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922554, Length=397, Percent_Identity=41.0579345088161, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 42794; Mature: 42663

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRDPTDTPCTAATAAVRAGIDRDTAYGAVTPPIVLSSNFSFDGFGNKRQYDYTRSGNP
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
TRDLLGEALAELEGGAGGVITSTGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALA
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
KKGHFALITADLTDPRSLADALAQSPKLVLIETPSNPLLRITDLRFVIEAAKKVGALTVV
CCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHHHHHHHHHCCEEEEE
DNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAELHQQLVWWANALGLT
CCCHHCHHHHCCHHCCCCEEEECCHHHCCCCCCHHCCCCEECCHHHHHHHHHHHHHCCCC
GSPFDAFLTLRGLRTLDARLRVHQENADAIAELLDGHAMVNQVYFPGLATHPGHALAARQ
CCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHH
QKGFGAMMSFELEGGEAAVRAFVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAA
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHH
AGISDGLLRLSIGIESAEDLLIDLRAGLSRAEATLTTTNRKKVDA
CCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHEEEEECCCEECCC
>Mature Secondary Structure 
SFRDPTDTPCTAATAAVRAGIDRDTAYGAVTPPIVLSSNFSFDGFGNKRQYDYTRSGNP
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
TRDLLGEALAELEGGAGGVITSTGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALA
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
KKGHFALITADLTDPRSLADALAQSPKLVLIETPSNPLLRITDLRFVIEAAKKVGALTVV
CCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHHHHHHHHHCCEEEEE
DNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAELHQQLVWWANALGLT
CCCHHCHHHHCCHHCCCCEEEECCHHHCCCCCCHHCCCCEECCHHHHHHHHHHHHHCCCC
GSPFDAFLTLRGLRTLDARLRVHQENADAIAELLDGHAMVNQVYFPGLATHPGHALAARQ
CCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHH
QKGFGAMMSFELEGGEAAVRAFVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAA
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHH
AGISDGLLRLSIGIESAEDLLIDLRAGLSRAEATLTTTNRKKVDA
CCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHEEEEECCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]