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The map label for this gene is ruvC [H]

Identifier: 84623201

GI number: 84623201

Start: 1700533

End: 1701057

Strand: Direct

Name: ruvC [H]

Synonym: XOO_1544

Alternate gene names: 84623201

Gene position: 1700533-1701057 (Clockwise)

Preceding gene: 84623200

Following gene: 84623202

Centisome position: 34.42

GC content: 66.1

Gene sequence:

>525_bases
ATGACCCGCATCCTGGGCATCGATCCCGGCTCGCAGCGCACCGGCATCGGCATCATCGATATCGACGAGGGCGGTCGCAG
CCGGCATGTGCACCACGCGCCGTTGATACTGCTGGGCGAGGGCGACTTCTCCCAGCGGCTCAAGCGCCTGTTGCACGGGC
TGGGCGAGTTGATCGAAACCTACCGGCCGGATGAGGTGGCGATCGAGAAGGTGTTCATGGGCAAGAGTGCCGCTTCCGCG
CTCAAGCTTGGCCAGGCGCGCGGCGCGGCAATCTGCGCGGTGGTCATGCGCGACCTGCCGGTGCACGAGTACGCCGCCAC
CGAGGTCAAGCTGGCGCTGGTCGGCAAAGGCGGCGCGGACAAGCTGCAGGTGCAGCACATGGTCGGCATCATGCTCAACT
TGAAAGGCAAGCTGCAGCCCGACGCCGCCGACGCACTGGCGGTCGCCATCACTCACGCCCACGTGCGCGCGACTGCGCAA
TGCTTGGGCGTCAATACCCAACAGGCCTGGAGCCGCAAGAAATGA

Upstream 100 bases:

>100_bases
GGCGGCATTCGGCATTGGGGATGCGCAACAGCGATGCGACCGGCACCCTGCACGTGCATTACCAATCCCTAACCTCCCAT
CCCCCAGTCCCGGCTTCTAG

Downstream 100 bases:

>100_bases
CAATCTGGATTCGGGATTCGGGATGGGGGATTCGCAGCGGCGAGTCCGCGCGCGCTGTGCCGCTTCGGCGGTGCGCAGCC
CTCGTCAACGGCAGTGAGGA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MTRILGIDPGSQRTGIGIIDIDEGGRSRHVHHAPLILLGEGDFSQRLKRLLHGLGELIETYRPDEVAIEKVFMGKSAASA
LKLGQARGAAICAVVMRDLPVHEYAATEVKLALVGKGGADKLQVQHMVGIMLNLKGKLQPDAADALAVAITHAHVRATAQ
CLGVNTQQAWSRKK

Sequences:

>Translated_174_residues
MTRILGIDPGSQRTGIGIIDIDEGGRSRHVHHAPLILLGEGDFSQRLKRLLHGLGELIETYRPDEVAIEKVFMGKSAASA
LKLGQARGAAICAVVMRDLPVHEYAATEVKLALVGKGGADKLQVQHMVGIMLNLKGKLQPDAADALAVAITHAHVRATAQ
CLGVNTQQAWSRKK
>Mature_173_residues
TRILGIDPGSQRTGIGIIDIDEGGRSRHVHHAPLILLGEGDFSQRLKRLLHGLGELIETYRPDEVAIEKVFMGKSAASAL
KLGQARGAAICAVVMRDLPVHEYAATEVKLALVGKGGADKLQVQHMVGIMLNLKGKLQPDAADALAVAITHAHVRATAQC
LGVNTQQAWSRKK

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=159, Percent_Identity=52.8301886792453, Blast_Score=127, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 18636; Mature: 18505

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRILGIDPGSQRTGIGIIDIDEGGRSRHVHHAPLILLGEGDFSQRLKRLLHGLGELIET
CCEEEECCCCCCCCCEEEEEECCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
YRPDEVAIEKVFMGKSAASALKLGQARGAAICAVVMRDLPVHEYAATEVKLALVGKGGAD
CCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHCEEEEEEEECCCCC
KLQVQHMVGIMLNLKGKLQPDAADALAVAITHAHVRATAQCLGVNTQQAWSRKK
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure 
TRILGIDPGSQRTGIGIIDIDEGGRSRHVHHAPLILLGEGDFSQRLKRLLHGLGELIET
CEEEECCCCCCCCCEEEEEECCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
YRPDEVAIEKVFMGKSAASALKLGQARGAAICAVVMRDLPVHEYAATEVKLALVGKGGAD
CCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHCEEEEEEEECCCCC
KLQVQHMVGIMLNLKGKLQPDAADALAVAITHAHVRATAQCLGVNTQQAWSRKK
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]