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The map label for this gene is rluF [H]

Identifier: 84623097

GI number: 84623097

Start: 1567584

End: 1568306

Strand: Direct

Name: rluF [H]

Synonym: XOO_1440

Alternate gene names: 84623097

Gene position: 1567584-1568306 (Clockwise)

Preceding gene: 84623096

Following gene: 84623100

Centisome position: 31.73

GC content: 63.21

Gene sequence:

>723_bases
ATGACGACGCGACTCAACAAATACATAGCCGAAACCGGCTTCTGCTCACGCCGCCAGGCCGATCGCTTGATCGGCGAACG
GCGGGTGACCGTCAATGGCGTGCCCGCCGGCACCGGCGCGGTGGTCGGCGAGGAAGACACCGTGCTGGTGGATGGCCAGC
CGCTGCGCGTGCGCGAGGCCAAGAAGCTCGGTGGCCGCAAACATGTCTACATCGCGCTCAACAAGCCGGTGGGCATCACC
TGCACCACCGAGAGTTCGGTCAAAGGCAATATCGTCGAATTCGTTGGCCACGAGCAGCGCATCTTCCCGATCGGCCGGCT
CGACAAGGAGTCCGAAGGGCTGATCCTGATGACCAGCAACGGCAACATCGTCAACGAGATTCTGCGCGCGGAAAATCGCC
ACAAGAAGGAATATCTGGTGGCGGTCAACAAGCCGGTCACCGACGAATTCCTGCGTGGCATGGCGCGCGGCGTGCGTATC
CACAACGAGACCACGCTGCCATGCCGCACCGCGCGCATCGCCAAATTCGGCTTCCGCATCGTGCTGGAGCAGGGCTTGAA
TCGGCAGATCCGCCTGATGGCCGCCGAGTTTGGCTATCGGGTCACGCAACTGCGCCGCGTGCGCATCGACAACATCAAGC
TGGCCGCGCTCAAGCCAGGGCAGTGGCGCAACCTCAGCGATGCGGAATTGCGCGGCCTGCTGCCGCAACGCACCGACTGG
TAA

Upstream 100 bases:

>100_bases
CCCATCTGGCGAGTACCAACTGCCGATTGCCGATTGCCGATTGCCGGTCTGGGCAGCTGGCATGGCGCACGGCCTGCGCT
AAATCGCCGATAATTGGCAG

Downstream 100 bases:

>100_bases
ACCCGGCAGACGCGCCGACCGCGCCGCCGCGTGTCTGTCGTCCGCCTGCTGATCACGCCGGCCTGGCAACGGCTCAAGAC
GAGCGCTGCAGCGCCCACGC

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTTRLNKYIAETGFCSRRQADRLIGERRVTVNGVPAGTGAVVGEEDTVLVDGQPLRVREAKKLGGRKHVYIALNKPVGIT
CTTESSVKGNIVEFVGHEQRIFPIGRLDKESEGLILMTSNGNIVNEILRAENRHKKEYLVAVNKPVTDEFLRGMARGVRI
HNETTLPCRTARIAKFGFRIVLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSDAELRGLLPQRTDW

Sequences:

>Translated_240_residues
MTTRLNKYIAETGFCSRRQADRLIGERRVTVNGVPAGTGAVVGEEDTVLVDGQPLRVREAKKLGGRKHVYIALNKPVGIT
CTTESSVKGNIVEFVGHEQRIFPIGRLDKESEGLILMTSNGNIVNEILRAENRHKKEYLVAVNKPVTDEFLRGMARGVRI
HNETTLPCRTARIAKFGFRIVLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSDAELRGLLPQRTDW
>Mature_239_residues
TTRLNKYIAETGFCSRRQADRLIGERRVTVNGVPAGTGAVVGEEDTVLVDGQPLRVREAKKLGGRKHVYIALNKPVGITC
TTESSVKGNIVEFVGHEQRIFPIGRLDKESEGLILMTSNGNIVNEILRAENRHKKEYLVAVNKPVTDEFLRGMARGVRIH
NETTLPCRTARIAKFGFRIVLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSDAELRGLLPQRTDW

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=53.6480686695279, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=34.453781512605, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787524, Length=247, Percent_Identity=29.9595141700405, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=28.2485875706215, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27054; Mature: 26923

Theoretical pI: Translated: 10.86; Mature: 10.86

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRLNKYIAETGFCSRRQADRLIGERRVTVNGVPAGTGAVVGEEDTVLVDGQPLRVREA
CCCHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCEECCCCEEEECCCCCEEHHH
KKLGGRKHVYIALNKPVGITCTTESSVKGNIVEFVGHEQRIFPIGRLDKESEGLILMTSN
HHCCCCEEEEEEECCCEEEEEECCCCCCCCCEEECCCCCEEEECCCCCCCCCCEEEEECC
GNIVNEILRAENRHKKEYLVAVNKPVTDEFLRGMARGVRIHNETTLPCRTARIAKFGFRI
CHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHH
VLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSDAELRGLLPQRTDW
EEHHCCCCEEEEEEHHHCHHHHHHHEEEECCEEEEEECCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTRLNKYIAETGFCSRRQADRLIGERRVTVNGVPAGTGAVVGEEDTVLVDGQPLRVREA
CCHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCEECCCCEEEECCCCCEEHHH
KKLGGRKHVYIALNKPVGITCTTESSVKGNIVEFVGHEQRIFPIGRLDKESEGLILMTSN
HHCCCCEEEEEEECCCEEEEEECCCCCCCCCEEECCCCCEEEECCCCCCCCCCEEEEECC
GNIVNEILRAENRHKKEYLVAVNKPVTDEFLRGMARGVRIHNETTLPCRTARIAKFGFRI
CHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHH
VLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSDAELRGLLPQRTDW
EEHHCCCCEEEEEEHHHCHHHHHHHEEEECCEEEEEECCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]