Click here to switch to the map view.

The map label for this gene is ddl [H]

Identifier: 84621981

GI number: 84621981

Start: 355636

End: 356739

Strand: Direct

Name: ddl [H]

Synonym: XOO_0324

Alternate gene names: 84621981

Gene position: 355636-356739 (Clockwise)

Preceding gene: 84621976

Following gene: 84621985

Centisome position: 7.2

GC content: 64.13

Gene sequence:

>1104_bases
ATGCGCAAGATCCGGGTCGGCCTCATCTTCGGCGGCAAATCGGCCGAGCACGAGGTTTCGTTGCAATCGGCGCGCAACAT
CCTGGATGCGCTGGACCCGCAACGCTTCGAGCCGGTGCTGATTGGTATCGACAAGCAAGGCCAATGGCACGTCAACGACC
CCGACAGCTTCCTGCTCCATGCCGACGACCCGGCACGTATTGCGCTGCATCGCAGTGGTCGCGGGGTGGCACTGCTGCCG
GGCGCGCAACAGCAGCAATTGCGGCCCATCCAGCCGGAGCAGGCGCTGGCGCAAATCGATGTGGTGTTCCCCATCGTGCA
CGGCACCTTGGGCGAAGACGGCTCGCTGCAGGGGCTGCTGCGCATGGCCAACCTGCCCTTCGTGGGCTCCGGCGTGCTGG
GCTCGGCGGTGGCGATGGACAAGGACATGGCCAAGCGCGTGTTGCGCGATGCCAGGCTGGCGGTGGCACCGTTCGTCTGC
TTCGACCGCCACACCGCCGCGCACGCCGATGTGGATACGTTGATCGCGCAGCTGGGCTTGCCGTTGTTCGTCAAACCGGC
CAACCAGGGCTCATCGGTCGGCGTCAGCCAGGTGCGCACGGCCGACGCATTCGCTGCTGCACTCGCCTTGGCGTTGGCCT
ATGACCATAAGGTGCTGGTGGAAGCGGCCGTTGCCGGGCGCGAAATCGAATGCGCCGTGCTGGGCAATGCCGTGCCACAC
GCAAGCGTCTGCGGCGAAGTGGTGGTGCACGACGCATTCTATTCGTACGCAACCAAATACATCAGCGAACACGGTGCCGA
GATCGTGATTCCGGCCGACATCGATGCGCAGACCCAGCAACGCATCCAGCAGATCGCAGTGCAGGCCTACCAGGCGCTGG
GCTGCGCAGGCATGGCGCGCGTGGACGTCTTTTTGTGCGCCGATGGGCGCATCGTGATCAACGAGGTCAACACGCTGCCA
GGCTTCACCCGCATCAGCATGTATCCGAAATTATGGCAGGCAAGCGGGCTGGACTATCGCGGTCTGATTACGCGGTTGAT
CGAGCTCGCACTGGAACGGCACACAGACGACCAACTGCTGCGCAGCGCGGTCGAGCTACACTGA

Upstream 100 bases:

>100_bases
CGGGCATGCCGGCGTTGTTTGCCATCGCTGGATGCGGCAAGGCTTCCTTCAGCGCATACGCCACAGGCATCCTGTCCGCT
TCCAATGGCAGGAGCAGGCA

Downstream 100 bases:

>100_bases
CCACGCTGCGGCAGCTGCACGCCGTACACATGCACGGCACGCCGGATTACCTGCACTCAACAATCCGCATGCCACCGAAC
TGCCGTTGCTCTACAGGGAA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetase A [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MRKIRVGLIFGGKSAEHEVSLQSARNILDALDPQRFEPVLIGIDKQGQWHVNDPDSFLLHADDPARIALHRSGRGVALLP
GAQQQQLRPIQPEQALAQIDVVFPIVHGTLGEDGSLQGLLRMANLPFVGSGVLGSAVAMDKDMAKRVLRDARLAVAPFVC
FDRHTAAHADVDTLIAQLGLPLFVKPANQGSSVGVSQVRTADAFAAALALALAYDHKVLVEAAVAGREIECAVLGNAVPH
ASVCGEVVVHDAFYSYATKYISEHGAEIVIPADIDAQTQQRIQQIAVQAYQALGCAGMARVDVFLCADGRIVINEVNTLP
GFTRISMYPKLWQASGLDYRGLITRLIELALERHTDDQLLRSAVELH

Sequences:

>Translated_367_residues
MRKIRVGLIFGGKSAEHEVSLQSARNILDALDPQRFEPVLIGIDKQGQWHVNDPDSFLLHADDPARIALHRSGRGVALLP
GAQQQQLRPIQPEQALAQIDVVFPIVHGTLGEDGSLQGLLRMANLPFVGSGVLGSAVAMDKDMAKRVLRDARLAVAPFVC
FDRHTAAHADVDTLIAQLGLPLFVKPANQGSSVGVSQVRTADAFAAALALALAYDHKVLVEAAVAGREIECAVLGNAVPH
ASVCGEVVVHDAFYSYATKYISEHGAEIVIPADIDAQTQQRIQQIAVQAYQALGCAGMARVDVFLCADGRIVINEVNTLP
GFTRISMYPKLWQASGLDYRGLITRLIELALERHTDDQLLRSAVELH
>Mature_367_residues
MRKIRVGLIFGGKSAEHEVSLQSARNILDALDPQRFEPVLIGIDKQGQWHVNDPDSFLLHADDPARIALHRSGRGVALLP
GAQQQQLRPIQPEQALAQIDVVFPIVHGTLGEDGSLQGLLRMANLPFVGSGVLGSAVAMDKDMAKRVLRDARLAVAPFVC
FDRHTAAHADVDTLIAQLGLPLFVKPANQGSSVGVSQVRTADAFAAALALALAYDHKVLVEAAVAGREIECAVLGNAVPH
ASVCGEVVVHDAFYSYATKYISEHGAEIVIPADIDAQTQQRIQQIAVQAYQALGCAGMARVDVFLCADGRIVINEVNTLP
GFTRISMYPKLWQASGLDYRGLITRLIELALERHTDDQLLRSAVELH

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=367, Percent_Identity=63.2152588555858, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1786280, Length=349, Percent_Identity=32.9512893982808, Blast_Score=178, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 39614; Mature: 39614

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIRVGLIFGGKSAEHEVSLQSARNILDALDPQRFEPVLIGIDKQGQWHVNDPDSFLLH
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEECCCCCCEEEE
ADDPARIALHRSGRGVALLPGAQQQQLRPIQPEQALAQIDVVFPIVHGTLGEDGSLQGLL
CCCCCEEEEEECCCCEEECCCCCHHCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHH
RMANLPFVGSGVLGSAVAMDKDMAKRVLRDARLAVAPFVCFDRHTAAHADVDTLIAQLGL
HHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCC
PLFVKPANQGSSVGVSQVRTADAFAAALALALAYDHKVLVEAAVAGREIECAVLGNAVPH
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCCC
ASVCGEVVVHDAFYSYATKYISEHGAEIVIPADIDAQTQQRIQQIAVQAYQALGCAGMAR
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VDVFLCADGRIVINEVNTLPGFTRISMYPKLWQASGLDYRGLITRLIELALERHTDDQLL
EEEEEECCCEEEEEECCCCCCCEEEHHCHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHH
RSAVELH
HHHHHCC
>Mature Secondary Structure
MRKIRVGLIFGGKSAEHEVSLQSARNILDALDPQRFEPVLIGIDKQGQWHVNDPDSFLLH
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEECCCCCCEEEE
ADDPARIALHRSGRGVALLPGAQQQQLRPIQPEQALAQIDVVFPIVHGTLGEDGSLQGLL
CCCCCEEEEEECCCCEEECCCCCHHCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHH
RMANLPFVGSGVLGSAVAMDKDMAKRVLRDARLAVAPFVCFDRHTAAHADVDTLIAQLGL
HHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCC
PLFVKPANQGSSVGVSQVRTADAFAAALALALAYDHKVLVEAAVAGREIECAVLGNAVPH
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCCC
ASVCGEVVVHDAFYSYATKYISEHGAEIVIPADIDAQTQQRIQQIAVQAYQALGCAGMAR
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VDVFLCADGRIVINEVNTLPGFTRISMYPKLWQASGLDYRGLITRLIELALERHTDDQLL
EEEEEECCCEEEEEECCCCCCCEEEHHCHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHH
RSAVELH
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]