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The map label for this gene is insI1 [C]

Identifier: 84621943

GI number: 84621943

Start: 312897

End: 313853

Strand: Direct

Name: insI1 [C]

Synonym: XOO_0286

Alternate gene names: 84621943

Gene position: 312897-313853 (Clockwise)

Preceding gene: 84621939

Following gene: 84621944

Centisome position: 6.33

GC content: 62.9

Gene sequence:

>957_bases
ATGTCATCCAGCCGCCTTGACCTATCGGAACGATACCGTCTGCATGCGTTATATGAAACCGGGATGTCGATGCGCGCCAT
CGCCGATGCAGTGGCGCGTGCGCCCAGCACGATCAGTCGTGAGCTGCGCCGCAACCGGCACGCGGCGAAGTATCGGCCCG
ATCACGCGCAGCGCATCAGCGAGCATCGGCGCGCACAGGCCAGCCGGCGACCACGCATCGACGCTGAGCGTATCCGTCAG
ATCGAGGTCCTGCTGAGGGAGGACTTCAGTCCCGAACAGATCGCCGGCCGCACCGGCTTGGCCAGTCACGCATGGATCTA
TCGGCACATCGACGCCGATCAGAAGCGCGGTGGTCAGTTGTTCATGCATCTACGCAAACGCCGCCGCAAGCGCCGTCGGC
GTGGCGTGCGCGATGGCCGCGGGCAGCTGACGCATCGGCGCAGCTGGACACAGCGCCCCAGTGTGGTTGAGCAGCGAAGC
CGTATCGGCGACTGGGAGCTGGAGACCATCAGGGCCTCACACGGACGCGCCGTGGTGGTCAGCATGACCGAACGCTGCAG
TGGCCTGCATCTGCTGGCTTACTCGCCCGACGGCACCGCCGAGAACGTGCGCAACGCCATTGTCCAGCGACTGGGCGGCC
TGCGCCATGCAGTTCACACCCTCACCGCCGACAACGGCAAGGAGTTCGCTGATCATCGGCTCATTGCCGCCTGCCTGCAG
AGCGATTTCTATTTCGCAGATCCGTACTGCCCATGGCAGCGCGGCAGCAACGAGAATGCCAACGGATTGACACGCCAATA
CTTGCCACGACAGACCGATTTCAGCACCATCACCGATGCGCACCTGCGATGGATCGAGCAGCGGCTCTACAATCGTCCGC
GCAAGATACTTGGATTCAAAACGCCCCTCGAAGTCTTCTCCGAGGAGGTCCTCAACAGCGTTGCGAATCAGAGTTGA

Upstream 100 bases:

>100_bases
CTTCGGCCGGGGCGGCTTCAACTCTGGATCGCAACACCAACGGTTGTGACGTGGTCCAGGCGACCTGACCTGAGCACTTC
ACCGCCAAGTGGAGTTGCCC

Downstream 100 bases:

>100_bases
ATTCGCCCCCTAATTTACTGACTTATTAGGGCCTGTTAACATTAATGTCTCGAGCGATTAAACTATTGGGCATGGAGATC
ACGCCAGCACAATTTGCACT

Product: IS1112 transposase

Products: NA

Alternate protein names: Transposon TN4551 [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSSSRLDLSERYRLHALYETGMSMRAIADAVARAPSTISRELRRNRHAAKYRPDHAQRISEHRRAQASRRPRIDAERIRQ
IEVLLREDFSPEQIAGRTGLASHAWIYRHIDADQKRGGQLFMHLRKRRRKRRRRGVRDGRGQLTHRRSWTQRPSVVEQRS
RIGDWELETIRASHGRAVVVSMTERCSGLHLLAYSPDGTAENVRNAIVQRLGGLRHAVHTLTADNGKEFADHRLIAACLQ
SDFYFADPYCPWQRGSNENANGLTRQYLPRQTDFSTITDAHLRWIEQRLYNRPRKILGFKTPLEVFSEEVLNSVANQS

Sequences:

>Translated_318_residues
MSSSRLDLSERYRLHALYETGMSMRAIADAVARAPSTISRELRRNRHAAKYRPDHAQRISEHRRAQASRRPRIDAERIRQ
IEVLLREDFSPEQIAGRTGLASHAWIYRHIDADQKRGGQLFMHLRKRRRKRRRRGVRDGRGQLTHRRSWTQRPSVVEQRS
RIGDWELETIRASHGRAVVVSMTERCSGLHLLAYSPDGTAENVRNAIVQRLGGLRHAVHTLTADNGKEFADHRLIAACLQ
SDFYFADPYCPWQRGSNENANGLTRQYLPRQTDFSTITDAHLRWIEQRLYNRPRKILGFKTPLEVFSEEVLNSVANQS
>Mature_317_residues
SSSRLDLSERYRLHALYETGMSMRAIADAVARAPSTISRELRRNRHAAKYRPDHAQRISEHRRAQASRRPRIDAERIRQI
EVLLREDFSPEQIAGRTGLASHAWIYRHIDADQKRGGQLFMHLRKRRRKRRRRGVRDGRGQLTHRRSWTQRPSVVEQRSR
IGDWELETIRASHGRAVVVSMTERCSGLHLLAYSPDGTAENVRNAIVQRLGGLRHAVHTLTADNGKEFADHRLIAACLQS
DFYFADPYCPWQRGSNENANGLTRQYLPRQTDFSTITDAHLRWIEQRLYNRPRKILGFKTPLEVFSEEVLNSVANQS

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=331, Percent_Identity=31.7220543806647, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790736, Length=331, Percent_Identity=31.7220543806647, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787671, Length=331, Percent_Identity=31.7220543806647, Blast_Score=131, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 36917; Mature: 36786

Theoretical pI: Translated: 11.49; Mature: 11.49

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSRLDLSERYRLHALYETGMSMRAIADAVARAPSTISRELRRNRHAAKYRPDHAQRIS
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHH
EHRRAQASRRPRIDAERIRQIEVLLREDFSPEQIAGRTGLASHAWIYRHIDADQKRGGQL
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHEECCCCHHCCHHH
FMHLRKRRRKRRRRGVRDGRGQLTHRRSWTQRPSVVEQRSRIGDWELETIRASHGRAVVV
HHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEE
SMTERCSGLHLLAYSPDGTAENVRNAIVQRLGGLRHAVHTLTADNGKEFADHRLIAACLQ
EEHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SDFYFADPYCPWQRGSNENANGLTRQYLPRQTDFSTITDAHLRWIEQRLYNRPRKILGFK
CCCEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCC
TPLEVFSEEVLNSVANQS
CHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSSRLDLSERYRLHALYETGMSMRAIADAVARAPSTISRELRRNRHAAKYRPDHAQRIS
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHH
EHRRAQASRRPRIDAERIRQIEVLLREDFSPEQIAGRTGLASHAWIYRHIDADQKRGGQL
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHEECCCCHHCCHHH
FMHLRKRRRKRRRRGVRDGRGQLTHRRSWTQRPSVVEQRSRIGDWELETIRASHGRAVVV
HHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEE
SMTERCSGLHLLAYSPDGTAENVRNAIVQRLGGLRHAVHTLTADNGKEFADHRLIAACLQ
EEHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SDFYFADPYCPWQRGSNENANGLTRQYLPRQTDFSTITDAHLRWIEQRLYNRPRKILGFK
CCCEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCC
TPLEVFSEEVLNSVANQS
CHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2820936 [H]