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The map label for this gene is mutM [H]

Identifier: 84621927

GI number: 84621927

Start: 300917

End: 301732

Strand: Direct

Name: mutM [H]

Synonym: XOO_0270

Alternate gene names: 84621927

Gene position: 300917-301732 (Clockwise)

Preceding gene: 84621921

Following gene: 84621929

Centisome position: 6.09

GC content: 58.33

Gene sequence:

>816_bases
ATGCCCGAGTTGCCCGAGGTTGAAACGACGCTGCGTGGCTTGTCGCCGCACCTGGTTGGGCAACGCATCCATGGCGTCAT
CCTGCGTCGACCTGATTTGCGCTGGCCGATTCCTGAGCAGATAGAGCGTCTGCTTCCCGGCGCCACCATCACCAATGTCC
GACGCCGTGCGAAATACTTACTGATCGACACGGATGCGGGCGGCAGCGCATTGCTGCATTTGGGGATGTCCGGAAGCCTG
CGGGTGCTACCTGGCGATACGTTGCCGCGGGCGCATGACCACGTGGATATCAGTTTGCAAAATGGACGCGTCCTGCGCTT
CAACGATCCACGTCGCTTTGGCTGTTTGCTGTGGCAATCCGACATCCAGGCGCATGAATTACTCGCTGCGCTTGGTCCAG
AACCATTATCGGAAGCATTTACTGGCGATTATCTGCACGCGCTTGCATATGGTCGGCGTGCTCCCGTTAAAACCTTTCTG
ATGGATCAGGCAGTGGTTGTCGGCGTTGGCAATATCTATGCTGCCGAAAGCCTGCATTGCGCTGGAATCAGCCCTCTGCG
CGAGGCTGGAAAGGTATCACTGGATCGTTATAGGCGGCTGGCTGCAGCGGTCAAGGACATCCTGTCGTACGCCATCCGAC
GCGGCGGTACTACCTTGCGTGATTTCATTAGCCCGGACGGTGCGCCCGGTTATTTCGAACAGGAGCTTACCGTCTACGGC
CGCGAAGGCGAGCCCTGCAAACAATGTGGACGAGTGTTGAAGCACGCGATGATCGGCCAACGCGCCACGGTGTGGTGTGG
GAGTTGTCAGCGGTAA

Upstream 100 bases:

>100_bases
AGATAAGAGCGTCTGCACGCAAGCGCCGATTGTATTTGAGTGTCCCGGCCTCCATATCCTGCTTTGCCGGTGCCTGACCG
GCAGTTCTTTGGACGCGCCG

Downstream 100 bases:

>100_bases
GGGCCACGCCATATCGACGCGGTCCTTGACGACTAGTTTGATAGCAGTTGTGGACAGATTTCCAGGCAAAAGCCCGGCAA
GCGGCCCGCAGCAACTTTCT

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MPELPEVETTLRGLSPHLVGQRIHGVILRRPDLRWPIPEQIERLLPGATITNVRRRAKYLLIDTDAGGSALLHLGMSGSL
RVLPGDTLPRAHDHVDISLQNGRVLRFNDPRRFGCLLWQSDIQAHELLAALGPEPLSEAFTGDYLHALAYGRRAPVKTFL
MDQAVVVGVGNIYAAESLHCAGISPLREAGKVSLDRYRRLAAAVKDILSYAIRRGGTTLRDFISPDGAPGYFEQELTVYG
REGEPCKQCGRVLKHAMIGQRATVWCGSCQR

Sequences:

>Translated_271_residues
MPELPEVETTLRGLSPHLVGQRIHGVILRRPDLRWPIPEQIERLLPGATITNVRRRAKYLLIDTDAGGSALLHLGMSGSL
RVLPGDTLPRAHDHVDISLQNGRVLRFNDPRRFGCLLWQSDIQAHELLAALGPEPLSEAFTGDYLHALAYGRRAPVKTFL
MDQAVVVGVGNIYAAESLHCAGISPLREAGKVSLDRYRRLAAAVKDILSYAIRRGGTTLRDFISPDGAPGYFEQELTVYG
REGEPCKQCGRVLKHAMIGQRATVWCGSCQR
>Mature_270_residues
PELPEVETTLRGLSPHLVGQRIHGVILRRPDLRWPIPEQIERLLPGATITNVRRRAKYLLIDTDAGGSALLHLGMSGSLR
VLPGDTLPRAHDHVDISLQNGRVLRFNDPRRFGCLLWQSDIQAHELLAALGPEPLSEAFTGDYLHALAYGRRAPVKTFLM
DQAVVVGVGNIYAAESLHCAGISPLREAGKVSLDRYRRLAAAVKDILSYAIRRGGTTLRDFISPDGAPGYFEQELTVYGR
EGEPCKQCGRVLKHAMIGQRATVWCGSCQR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1790066, Length=271, Percent_Identity=52.0295202952029, Blast_Score=287, Evalue=5e-79,
Organism=Escherichia coli, GI1786932, Length=275, Percent_Identity=26.5454545454545, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663 [H]

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS [H]

EC number: =3.2.2.23; =4.2.99.18 [H]

Molecular weight: Translated: 29915; Mature: 29784

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS51066 ZF_FPG_2 ; PS51068 FPG_CAT ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETTLRGLSPHLVGQRIHGVILRRPDLRWPIPEQIERLLPGATITNVRRRAKYL
CCCCCCHHHHHHCCCHHHHHHHHHEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHCCEEE
LIDTDAGGSALLHLGMSGSLRVLPGDTLPRAHDHVDISLQNGRVLRFNDPRRFGCLLWQS
EEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEECCCEEEEECCCCHHEEEEEHH
DIQAHELLAALGPEPLSEAFTGDYLHALAYGRRAPVKTFLMDQAVVVGVGNIYAAESLHC
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEECCCCHHHCCCCC
AGISPLREAGKVSLDRYRRLAAAVKDILSYAIRRGGTTLRDFISPDGAPGYFEQELTVYG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEEEEC
REGEPCKQCGRVLKHAMIGQRATVWCGSCQR
CCCCHHHHHHHHHHHHHHCCCCEEECCCCCC
>Mature Secondary Structure 
PELPEVETTLRGLSPHLVGQRIHGVILRRPDLRWPIPEQIERLLPGATITNVRRRAKYL
CCCCCHHHHHHCCCHHHHHHHHHEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHCCEEE
LIDTDAGGSALLHLGMSGSLRVLPGDTLPRAHDHVDISLQNGRVLRFNDPRRFGCLLWQS
EEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEECCCEEEEECCCCHHEEEEEHH
DIQAHELLAALGPEPLSEAFTGDYLHALAYGRRAPVKTFLMDQAVVVGVGNIYAAESLHC
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEECCCCHHHCCCCC
AGISPLREAGKVSLDRYRRLAAAVKDILSYAIRRGGTTLRDFISPDGAPGYFEQELTVYG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEEEEC
REGEPCKQCGRVLKHAMIGQRATVWCGSCQR
CCCCHHHHHHHHHHHHHHCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]