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The map label for this gene is plsB [H]

Identifier: 84621889

GI number: 84621889

Start: 258423

End: 261083

Strand: Direct

Name: plsB [H]

Synonym: XOO_0232

Alternate gene names: 84621889

Gene position: 258423-261083 (Clockwise)

Preceding gene: 84621888

Following gene: 84621890

Centisome position: 5.23

GC content: 65.73

Gene sequence:

>2661_bases
ATGACGGTGATGCCAGAACAGAATCCCCTGCCGTTTCCGGACGGCCCGCCCGCCCCGCCCAGCGCTGCCGTCGCCGACAC
CGGTGCCACGACAGCGGCGTTGCCGCTCGCCGAACCGGTACCGCCTGAGGTTGCGCCCTCGATCGCGCCCGCCGTCGCCG
CTGCCCGCAGCGCCAAACGCCCGTGGTGGGCGCGCCTGCTCGGCCGTCTGGCCGACCCGTGGCTGGGCCTGACCATCGAG
CCGGACCAACCCGGCCGCTACGATGACGGCCGCCCGGTGGTGTACGTGCTGGAAGACTACGGCATGTCCAATGCGTTGAT
TCTGGACAAGGCCTGCCGCGAAGTCGGTCTGCCGTCGCCGCTGGTGCCGTTGCCGGGCGACCCGCTGGAGCGCAAGCGCG
CCTACCTGGCGCTCTCGAGACGCAGCAGCAATTCGCTGATTCCCGAACAGCGTGGCGGCAAGACCCATTCGGATTCGCTG
GCCAAGTTGCTGCAGGCGCATCGCGTGCGCACCGACCTGGACGTACATCTGGTGCCGGTGTCGATCTTCGTCGGCCGCGC
GCCGGACAAGCAGAGCGGCTGGTTCGCCGTGCTGTTCTCGGAAAACTGGGCGCTGGTCGGCCGCTTCCGCCGCCTGCTCT
CGGTATTGCTCAACGGCCGCACCACCATCGTGCGGTTCGCCCCGCCGATCTCGCTGCGCCAGACCATGGCCGAAGGGCTG
CCGCCCGAGCGCACCTTGCGCAAGTTGCAGCGCGTGTTGCGCACGCACTTCCGGCGCATCCGCGAGGCGGTGATCGGCCC
GGACCTGTCCACCCGCCGTCTGCTGGTGGACCAGGTGCTGGCCGCCGAGTCGGTGCGCGAGGCCATCGCCGTCCAGGCCA
AGCGCGACAACTCCAAGCCGGTCGATGCCTGGCGCAAGGCGCATGCCTATGCGTGGGAAATTGCCGCCGACTATTCCAGC
CCGGTGGTGCGCTCGGCCAGGTTCCTGCTCACCCACGTGTGGAACCGCATCTACGCCGGCGTGCTGGTGCATCACCTGGA
CAAGCTCAAACAGGCCGCGCCCGGGCACGAAGTGGTGTACGTGCCCAGTCACCGCAGCCATATGGATTACCTGCTGCTGA
GCTACGTGCTGTACGAGCGCGGCATCGTGCCGCCGCATATCGTTGCCGGCATCAACCTCAATCTGCCGGTGGTCGGCACG
CTGCTGCGCAAGGGCGGCGCGTTCTTCATCCGCCGCTCGATCAAGGGCAATGCGCTGTATTCGGCAGTGCTCAGCGAATA
CGTCGCCCAGCTGGTGGCCGGCGGCTATTCCATCGAATACTTCGTCGAAGGCGGGCGCTCGCGCACCGGGCGTTTGCTGC
AGCCCAAGGGCGGCATGATCGCGATGACGCTGCGCGCGTATCTGCGTCAACCGCGCAAGCCGGTGCTGTTCCAGCCGGTG
TACATCGGCTACGAGAAGCTGATGGAAGGCAACAGCTACCTCGACGAACTCACCGGGCGGCCGAAGGAAAAGGAATCCAT
CTGGGGCCTGTTGTGGTCCATCCCCAAGGTGCTCAAGCAGAACTACGGCCAGGTGGTAGTGAACTTCGGCGAGCCGATCG
CGCTCAACGACGTGCTGGCCAAGCACGCACCCGACTGGGACGGCCAGCCGCTGCCGGAAGACGAGAAGCCCACCTGGCTG
GCGCCGGCAGTGGACACCCTGTCCACGCAGATCCAGACCCGCATCAACTGCGCCGCCGACGTCAATCCGATCAATTTGCT
TGCGCTGGCATTGTTGTCCACGCCCAAGCATGCGATGGGCGAAGCCGATCTGATTGCGCAGATCGAGCTGTGCAAGAAGT
TGCTGGCGGAAATGCCGTATTCGGACCGCGTCACCGTCACCCCGCATACGCCAGCGCGCATCATCACCCACGCCGAAGAA
ATCAACGTGCTCACGCGCGTGTCGCATCCGCTGGGCGATGTACTCAGCGTCAGCGGCGACACCGCGGTGCTGCTGAGCTA
TTTCCGCAACAACGTGCTGCATCTGTTTACGGCATCGTCGTGGGTGGCGTGCTGCTTCCAGAACAACCGCCGCATGAGCC
GCGCCGGTCTGTTGCGTCTGGGCCGCACGGTGTACCCGTTCCTGCAGGCCGAATTGTTCCTGCCGTGGAGCGAGGATCGC
TTTGCCGAACGCATCGAGCAGATCATCGACATGTTCGTGCGCGAAGGCCTGCTGCTCAACGTCACCGACGACGATGGCGG
CATCCTGGCGCGTAATACCGGGCAGACCGATGAAGTGTTCCGTCTGCGTGCGATCGGCCATTCGCTGCAGCAGGCCTTCG
AGCGCTATTACATCGCCATTTCGGTGCTGGTGAAGAACGGCCCCGGCGTGCTCGGCGCCGGCGAACTGGAAAGCCTGTGC
CAACAGGCCGCGCAACGTCTGAGCCTGTTGTACGCACCGGCCGCGCCGGAGTTCTTCGACAAGACCCTGTTCCGCAGCTT
CATCCAGAAACTGCGCGAGCTGGGCCTGGTATGGCCGGACGAGAACAGCAAGCTGATGTTCGACGAGCGCCTGGATGCCT
GGGCCAAGGATGCCAAGTTCATTCTCGGCCGCGAACTGCGCCACACCATCGAGCGGGTCAGCCCGGAAGCGGCCAAACCG
GACGTGCTGCCGCCGCCGTAG

Upstream 100 bases:

>100_bases
TCAACGGCATCCAGGCCGCGCCCTTACCCAACGCCACTGTAGCGCCCGACACCGCAGCGGCCGCGCCTGCCGACACCGGG
CAACGCCCGGCTGGGCGATA

Downstream 100 bases:

>100_bases
TAAGCCGCTAGCGAAGGTCTTCAAGTCCTGCGGCGAGCGTGGGCCGCGGGATGACGCGTCCGGGGGGCACGCGATGGCGG
CCTTCACATAACGGTTCCGT

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT [H]

Number of amino acids: Translated: 886; Mature: 885

Protein sequence:

>886_residues
MTVMPEQNPLPFPDGPPAPPSAAVADTGATTAALPLAEPVPPEVAPSIAPAVAAARSAKRPWWARLLGRLADPWLGLTIE
PDQPGRYDDGRPVVYVLEDYGMSNALILDKACREVGLPSPLVPLPGDPLERKRAYLALSRRSSNSLIPEQRGGKTHSDSL
AKLLQAHRVRTDLDVHLVPVSIFVGRAPDKQSGWFAVLFSENWALVGRFRRLLSVLLNGRTTIVRFAPPISLRQTMAEGL
PPERTLRKLQRVLRTHFRRIREAVIGPDLSTRRLLVDQVLAAESVREAIAVQAKRDNSKPVDAWRKAHAYAWEIAADYSS
PVVRSARFLLTHVWNRIYAGVLVHHLDKLKQAAPGHEVVYVPSHRSHMDYLLLSYVLYERGIVPPHIVAGINLNLPVVGT
LLRKGGAFFIRRSIKGNALYSAVLSEYVAQLVAGGYSIEYFVEGGRSRTGRLLQPKGGMIAMTLRAYLRQPRKPVLFQPV
YIGYEKLMEGNSYLDELTGRPKEKESIWGLLWSIPKVLKQNYGQVVVNFGEPIALNDVLAKHAPDWDGQPLPEDEKPTWL
APAVDTLSTQIQTRINCAADVNPINLLALALLSTPKHAMGEADLIAQIELCKKLLAEMPYSDRVTVTPHTPARIITHAEE
INVLTRVSHPLGDVLSVSGDTAVLLSYFRNNVLHLFTASSWVACCFQNNRRMSRAGLLRLGRTVYPFLQAELFLPWSEDR
FAERIEQIIDMFVREGLLLNVTDDDGGILARNTGQTDEVFRLRAIGHSLQQAFERYYIAISVLVKNGPGVLGAGELESLC
QQAAQRLSLLYAPAAPEFFDKTLFRSFIQKLRELGLVWPDENSKLMFDERLDAWAKDAKFILGRELRHTIERVSPEAAKP
DVLPPP

Sequences:

>Translated_886_residues
MTVMPEQNPLPFPDGPPAPPSAAVADTGATTAALPLAEPVPPEVAPSIAPAVAAARSAKRPWWARLLGRLADPWLGLTIE
PDQPGRYDDGRPVVYVLEDYGMSNALILDKACREVGLPSPLVPLPGDPLERKRAYLALSRRSSNSLIPEQRGGKTHSDSL
AKLLQAHRVRTDLDVHLVPVSIFVGRAPDKQSGWFAVLFSENWALVGRFRRLLSVLLNGRTTIVRFAPPISLRQTMAEGL
PPERTLRKLQRVLRTHFRRIREAVIGPDLSTRRLLVDQVLAAESVREAIAVQAKRDNSKPVDAWRKAHAYAWEIAADYSS
PVVRSARFLLTHVWNRIYAGVLVHHLDKLKQAAPGHEVVYVPSHRSHMDYLLLSYVLYERGIVPPHIVAGINLNLPVVGT
LLRKGGAFFIRRSIKGNALYSAVLSEYVAQLVAGGYSIEYFVEGGRSRTGRLLQPKGGMIAMTLRAYLRQPRKPVLFQPV
YIGYEKLMEGNSYLDELTGRPKEKESIWGLLWSIPKVLKQNYGQVVVNFGEPIALNDVLAKHAPDWDGQPLPEDEKPTWL
APAVDTLSTQIQTRINCAADVNPINLLALALLSTPKHAMGEADLIAQIELCKKLLAEMPYSDRVTVTPHTPARIITHAEE
INVLTRVSHPLGDVLSVSGDTAVLLSYFRNNVLHLFTASSWVACCFQNNRRMSRAGLLRLGRTVYPFLQAELFLPWSEDR
FAERIEQIIDMFVREGLLLNVTDDDGGILARNTGQTDEVFRLRAIGHSLQQAFERYYIAISVLVKNGPGVLGAGELESLC
QQAAQRLSLLYAPAAPEFFDKTLFRSFIQKLRELGLVWPDENSKLMFDERLDAWAKDAKFILGRELRHTIERVSPEAAKP
DVLPPP
>Mature_885_residues
TVMPEQNPLPFPDGPPAPPSAAVADTGATTAALPLAEPVPPEVAPSIAPAVAAARSAKRPWWARLLGRLADPWLGLTIEP
DQPGRYDDGRPVVYVLEDYGMSNALILDKACREVGLPSPLVPLPGDPLERKRAYLALSRRSSNSLIPEQRGGKTHSDSLA
KLLQAHRVRTDLDVHLVPVSIFVGRAPDKQSGWFAVLFSENWALVGRFRRLLSVLLNGRTTIVRFAPPISLRQTMAEGLP
PERTLRKLQRVLRTHFRRIREAVIGPDLSTRRLLVDQVLAAESVREAIAVQAKRDNSKPVDAWRKAHAYAWEIAADYSSP
VVRSARFLLTHVWNRIYAGVLVHHLDKLKQAAPGHEVVYVPSHRSHMDYLLLSYVLYERGIVPPHIVAGINLNLPVVGTL
LRKGGAFFIRRSIKGNALYSAVLSEYVAQLVAGGYSIEYFVEGGRSRTGRLLQPKGGMIAMTLRAYLRQPRKPVLFQPVY
IGYEKLMEGNSYLDELTGRPKEKESIWGLLWSIPKVLKQNYGQVVVNFGEPIALNDVLAKHAPDWDGQPLPEDEKPTWLA
PAVDTLSTQIQTRINCAADVNPINLLALALLSTPKHAMGEADLIAQIELCKKLLAEMPYSDRVTVTPHTPARIITHAEEI
NVLTRVSHPLGDVLSVSGDTAVLLSYFRNNVLHLFTASSWVACCFQNNRRMSRAGLLRLGRTVYPFLQAELFLPWSEDRF
AERIEQIIDMFVREGLLLNVTDDDGGILARNTGQTDEVFRLRAIGHSLQQAFERYYIAISVLVKNGPGVLGAGELESLCQ
QAAQRLSLLYAPAAPEFFDKTLFRSFIQKLRELGLVWPDENSKLMFDERLDAWAKDAKFILGRELRHTIERVSPEAAKPD
VLPPP

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family [H]

Homologues:

Organism=Homo sapiens, GI7657134, Length=469, Percent_Identity=30.7036247334755, Blast_Score=179, Evalue=7e-45,
Organism=Homo sapiens, GI190358539, Length=565, Percent_Identity=24.070796460177, Blast_Score=153, Evalue=6e-37,
Organism=Escherichia coli, GI87082362, Length=753, Percent_Identity=38.9110225763612, Blast_Score=489, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI25147672, Length=263, Percent_Identity=34.2205323193916, Blast_Score=154, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71988723, Length=315, Percent_Identity=32.0634920634921, Blast_Score=146, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71988728, Length=191, Percent_Identity=39.7905759162304, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17864692, Length=270, Percent_Identity=34.0740740740741, Blast_Score=159, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21357731, Length=214, Percent_Identity=36.9158878504673, Blast_Score=150, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24650752, Length=214, Percent_Identity=36.9158878504673, Blast_Score=150, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24650754, Length=205, Percent_Identity=36.5853658536585, Blast_Score=150, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR022284 [H]

Pfam domain/function: PF01553 Acyltransferase [H]

EC number: =2.3.1.15 [H]

Molecular weight: Translated: 98646; Mature: 98515

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVMPEQNPLPFPDGPPAPPSAAVADTGATTAALPLAEPVPPEVAPSIAPAVAAARSAKR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCC
PWWARLLGRLADPWLGLTIEPDQPGRYDDGRPVVYVLEDYGMSNALILDKACREVGLPSP
CHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCC
LVPLPGDPLERKRAYLALSRRSSNSLIPEQRGGKTHSDSLAKLLQAHRVRTDLDVHLVPV
CCCCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
SIFVGRAPDKQSGWFAVLFSENWALVGRFRRLLSVLLNGRTTIVRFAPPISLRQTMAEGL
EEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCC
PPERTLRKLQRVLRTHFRRIREAVIGPDLSTRRLLVDQVLAAESVREAIAVQAKRDNSKP
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VDAWRKAHAYAWEIAADYSSPVVRSARFLLTHVWNRIYAGVLVHHLDKLKQAAPGHEVVY
HHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VPSHRSHMDYLLLSYVLYERGIVPPHIVAGINLNLPVVGTLLRKGGAFFIRRSIKGNALY
ECCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHCCCCEEEEECCCCHHHH
SAVLSEYVAQLVAGGYSIEYFVEGGRSRTGRLLQPKGGMIAMTLRAYLRQPRKPVLFQPV
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEECCCCCEEHHHHHHHHHCCCCCEEECHH
YIGYEKLMEGNSYLDELTGRPKEKESIWGLLWSIPKVLKQNYGQVVVNFGEPIALNDVLA
HHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHH
KHAPDWDGQPLPEDEKPTWLAPAVDTLSTQIQTRINCAADVNPINLLALALLSTPKHAMG
HCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHCC
EADLIAQIELCKKLLAEMPYSDRVTVTPHTPARIITHAEEINVLTRVSHPLGDVLSVSGD
CHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
TAVLLSYFRNNVLHLFTASSWVACCFQNNRRMSRAGLLRLGRTVYPFLQAELFLPWSEDR
HHHHHHHHHCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHH
FAERIEQIIDMFVREGLLLNVTDDDGGILARNTGQTDEVFRLRAIGHSLQQAFERYYIAI
HHHHHHHHHHHHHHCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEEE
SVLVKNGPGVLGAGELESLCQQAAQRLSLLYAPAAPEFFDKTLFRSFIQKLRELGLVWPD
EEEEECCCCCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
ENSKLMFDERLDAWAKDAKFILGRELRHTIERVSPEAAKPDVLPPP
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVMPEQNPLPFPDGPPAPPSAAVADTGATTAALPLAEPVPPEVAPSIAPAVAAARSAKR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCC
PWWARLLGRLADPWLGLTIEPDQPGRYDDGRPVVYVLEDYGMSNALILDKACREVGLPSP
CHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCC
LVPLPGDPLERKRAYLALSRRSSNSLIPEQRGGKTHSDSLAKLLQAHRVRTDLDVHLVPV
CCCCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
SIFVGRAPDKQSGWFAVLFSENWALVGRFRRLLSVLLNGRTTIVRFAPPISLRQTMAEGL
EEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCC
PPERTLRKLQRVLRTHFRRIREAVIGPDLSTRRLLVDQVLAAESVREAIAVQAKRDNSKP
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VDAWRKAHAYAWEIAADYSSPVVRSARFLLTHVWNRIYAGVLVHHLDKLKQAAPGHEVVY
HHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VPSHRSHMDYLLLSYVLYERGIVPPHIVAGINLNLPVVGTLLRKGGAFFIRRSIKGNALY
ECCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHCCCCEEEEECCCCHHHH
SAVLSEYVAQLVAGGYSIEYFVEGGRSRTGRLLQPKGGMIAMTLRAYLRQPRKPVLFQPV
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEECCCCCEEHHHHHHHHHCCCCCEEECHH
YIGYEKLMEGNSYLDELTGRPKEKESIWGLLWSIPKVLKQNYGQVVVNFGEPIALNDVLA
HHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHH
KHAPDWDGQPLPEDEKPTWLAPAVDTLSTQIQTRINCAADVNPINLLALALLSTPKHAMG
HCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHCC
EADLIAQIELCKKLLAEMPYSDRVTVTPHTPARIITHAEEINVLTRVSHPLGDVLSVSGD
CHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
TAVLLSYFRNNVLHLFTASSWVACCFQNNRRMSRAGLLRLGRTVYPFLQAELFLPWSEDR
HHHHHHHHHCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHH
FAERIEQIIDMFVREGLLLNVTDDDGGILARNTGQTDEVFRLRAIGHSLQQAFERYYIAI
HHHHHHHHHHHHHHCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEEE
SVLVKNGPGVLGAGELESLCQQAAQRLSLLYAPAAPEFFDKTLFRSFIQKLRELGLVWPD
EEEEECCCCCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
ENSKLMFDERLDAWAKDAKFILGRELRHTIERVSPEAAKPDVLPPP
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]