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The map label for this gene is argG

Identifier: 84490240

GI number: 84490240

Start: 1624541

End: 1625719

Strand: Direct

Name: argG

Synonym: Msp_1459

Alternate gene names: 84490240

Gene position: 1624541-1625719 (Clockwise)

Preceding gene: 84490216

Following gene: 84490250

Centisome position: 91.92

GC content: 31.89

Gene sequence:

>1179_bases
ATGGAAAAAGTAGTATTAGCATTCAGTGGAGGATTAGACACCTCAGTATGTGTTAAATTATTACAAGAAAAATATAATAT
GGAAGTTATCACAGCATGTGTAGATGTAGGACAACCAGAAGATGAAATTAAAAAACCAGAAGCAGCAGCTCAAAAAATTG
GTTCAGACAAACACTACACAATAGATGCAAAAGATGAATTTGCAAAAGAATATGCATTCAGATGTATAAAAACAAATTCA
TTATATGAAGGATACCCATTAAGTACAGCATATGCAAGACCATTAATTGCAAAAAAAATAGTTGAAATAGCAAAAAAAGA
AAATGCTTCAACAATTGCACATGGATGTACTGGAAAAGGAAACGATCAATTCAGATTTGAAGCAACCATCAGATCAGAAT
CTGATCTAGACATAGTTGCTCCTGTACGTGATTATAACCTAACAAGAACAGAAGAACAAGACTATGCACGAGAACATGGA
ATACCTCTTCCATCGGAAAAAATATACAGTATAGATGAAAACCTATGGGGTAGATCAATAGAAGGAGGATTACTTGAAAA
TCCTGCAACAGCTGCACCAGAAGAAATATATGAATGGACAAAATCCACAGTAGATGCACCAGAAACTCCACAAATTGTTA
AAATAGAATTTGTTAATGGAGAACCTGTAAAAATCAATGATGAAGAATTAGAACCTGTAGCTCTTATTAGAAAAGCAAAT
GAAATTGCTGGTAACAATGGAATTGGTAGAATAGATATGGTTGAAGACAGAATTATTGGTCTTAAATCAAGAGAAAACTA
TGAAACACCAGGCGCTATTCTTCTTATAACAGCACATGCTGCTCTTGAAAAATTAATATTCAGTAGAGAAGAAATTAAAT
TTGCAGAAAACTTAAGTCAAAAATATGCTGAATTAGTATATAATGGTCTTTGGAATGAACCATTAAGAGAAGATCTTGAT
GTTGTAAATGATCATATGCAACAAAGAGTAACAGGTACTGTTGAAGTAAGATTACATAAAGGTTCAATGAAAGTTATTAC
AAAAGAATCTCCTTATGCATTATATAATGAAGAAAATGTATCATTTGAAGATAAAGGAATTGATCAAAGAGAAATGATTG
GTATGGTAAAATACTACGATCTTCAAGGTGCAGAATACTCTAAAGTAAAAAGAGAATAG

Upstream 100 bases:

>100_bases
ACAAACAGATATATAATAATATATATCTCAATACACATAGAGAAATATTACTTTTATAAAAAAAAATATTAATTTAATTA
TAAAATAAAAAGGTGAAACA

Downstream 100 bases:

>100_bases
ATACCCTATTTTCAATTACTTTTTTTATCCAAAATAAACACTCTTTCTAAAAAAAATTACTATAAAAACTAGAATATATT
AAAAAAAATAGTTAAGGGAG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MEKVVLAFSGGLDTSVCVKLLQEKYNMEVITACVDVGQPEDEIKKPEAAAQKIGSDKHYTIDAKDEFAKEYAFRCIKTNS
LYEGYPLSTAYARPLIAKKIVEIAKKENASTIAHGCTGKGNDQFRFEATIRSESDLDIVAPVRDYNLTRTEEQDYAREHG
IPLPSEKIYSIDENLWGRSIEGGLLENPATAAPEEIYEWTKSTVDAPETPQIVKIEFVNGEPVKINDEELEPVALIRKAN
EIAGNNGIGRIDMVEDRIIGLKSRENYETPGAILLITAHAALEKLIFSREEIKFAENLSQKYAELVYNGLWNEPLREDLD
VVNDHMQQRVTGTVEVRLHKGSMKVITKESPYALYNEENVSFEDKGIDQREMIGMVKYYDLQGAEYSKVKRE

Sequences:

>Translated_392_residues
MEKVVLAFSGGLDTSVCVKLLQEKYNMEVITACVDVGQPEDEIKKPEAAAQKIGSDKHYTIDAKDEFAKEYAFRCIKTNS
LYEGYPLSTAYARPLIAKKIVEIAKKENASTIAHGCTGKGNDQFRFEATIRSESDLDIVAPVRDYNLTRTEEQDYAREHG
IPLPSEKIYSIDENLWGRSIEGGLLENPATAAPEEIYEWTKSTVDAPETPQIVKIEFVNGEPVKINDEELEPVALIRKAN
EIAGNNGIGRIDMVEDRIIGLKSRENYETPGAILLITAHAALEKLIFSREEIKFAENLSQKYAELVYNGLWNEPLREDLD
VVNDHMQQRVTGTVEVRLHKGSMKVITKESPYALYNEENVSFEDKGIDQREMIGMVKYYDLQGAEYSKVKRE
>Mature_392_residues
MEKVVLAFSGGLDTSVCVKLLQEKYNMEVITACVDVGQPEDEIKKPEAAAQKIGSDKHYTIDAKDEFAKEYAFRCIKTNS
LYEGYPLSTAYARPLIAKKIVEIAKKENASTIAHGCTGKGNDQFRFEATIRSESDLDIVAPVRDYNLTRTEEQDYAREHG
IPLPSEKIYSIDENLWGRSIEGGLLENPATAAPEEIYEWTKSTVDAPETPQIVKIEFVNGEPVKINDEELEPVALIRKAN
EIAGNNGIGRIDMVEDRIIGLKSRENYETPGAILLITAHAALEKLIFSREEIKFAENLSQKYAELVYNGLWNEPLREDLD
VVNDHMQQRVTGTVEVRLHKGSMKVITKESPYALYNEENVSFEDKGIDQREMIGMVKYYDLQGAEYSKVKRE

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=402, Percent_Identity=40.547263681592, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI16950633, Length=402, Percent_Identity=40.547263681592, Blast_Score=264, Evalue=1e-70,
Organism=Escherichia coli, GI1789563, Length=382, Percent_Identity=26.4397905759162, Blast_Score=104, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324514, Length=367, Percent_Identity=36.7847411444142, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI21358151, Length=383, Percent_Identity=38.9033942558747, Blast_Score=236, Evalue=3e-62,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_METST (Q2NEC4)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_448472.1
- HSSP:   Q9X2A1
- ProteinModelPortal:   Q2NEC4
- SMR:   Q2NEC4
- STRING:   Q2NEC4
- GeneID:   3856091
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_1459
- NMPDR:   fig|339860.6.peg.1394
- eggNOG:   arNOG04108
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK13820
- BioCyc:   MSTA339860:MSP_1459-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44224; Mature: 44224

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 85-85 BINDING 115-115 BINDING 117-117 BINDING 121-121 BINDING 121-121 BINDING 122-122 BINDING 125-125 BINDING 170-170 BINDING 179-179 BINDING 255-255 BINDING 267-267

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVVLAFSGGLDTSVCVKLLQEKYNMEVITACVDVGQPEDEIKKPEAAAQKIGSDKHYT
CCEEEEEECCCCCHHHHHHHHHHHHCHHEEEHHHHCCCCHHHHCCCHHHHHHCCCCCEEE
IDAKDEFAKEYAFRCIKTNSLYEGYPLSTAYARPLIAKKIVEIAKKENASTIAHGCTGKG
ECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC
NDQFRFEATIRSESDLDIVAPVRDYNLTRTEEQDYAREHGIPLPSEKIYSIDENLWGRSI
CCCEEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCCCHHHEECCHHHCCCCC
EGGLLENPATAAPEEIYEWTKSTVDAPETPQIVKIEFVNGEPVKINDEELEPVALIRKAN
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHH
EIAGNNGIGRIDMVEDRIIGLKSRENYETPGAILLITAHAALEKLIFSREEIKFAENLSQ
HHCCCCCCCEEEEHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
KYAELVYNGLWNEPLREDLDVVNDHMQQRVTGTVEVRLHKGSMKVITKESPYALYNEENV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCEEEEECCCCEEEECCCCC
SFEDKGIDQREMIGMVKYYDLQGAEYSKVKRE
CCCCCCCCHHHHHHHHHEEECCCCCHHHHCCC
>Mature Secondary Structure
MEKVVLAFSGGLDTSVCVKLLQEKYNMEVITACVDVGQPEDEIKKPEAAAQKIGSDKHYT
CCEEEEEECCCCCHHHHHHHHHHHHCHHEEEHHHHCCCCHHHHCCCHHHHHHCCCCCEEE
IDAKDEFAKEYAFRCIKTNSLYEGYPLSTAYARPLIAKKIVEIAKKENASTIAHGCTGKG
ECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC
NDQFRFEATIRSESDLDIVAPVRDYNLTRTEEQDYAREHGIPLPSEKIYSIDENLWGRSI
CCCEEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCCCHHHEECCHHHCCCCC
EGGLLENPATAAPEEIYEWTKSTVDAPETPQIVKIEFVNGEPVKINDEELEPVALIRKAN
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHH
EIAGNNGIGRIDMVEDRIIGLKSRENYETPGAILLITAHAALEKLIFSREEIKFAENLSQ
HHCCCCCCCEEEEHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
KYAELVYNGLWNEPLREDLDVVNDHMQQRVTGTVEVRLHKGSMKVITKESPYALYNEENV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCEEEEECCCCEEEECCCCC
SFEDKGIDQREMIGMVKYYDLQGAEYSKVKRE
CCCCCCCCHHHHHHHHHEEECCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA