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The map label for this gene is cysG [C]

Identifier: 84490189

GI number: 84490189

Start: 1581554

End: 1582186

Strand: Direct

Name: cysG [C]

Synonym: Msp_1407

Alternate gene names: 84490189

Gene position: 1581554-1582186 (Clockwise)

Preceding gene: 84490188

Following gene: 84490190

Centisome position: 89.48

GC content: 27.17

Gene sequence:

>633_bases
ATGACAGTTACATCATTGTTTTTAGATATGGAAGAGAAAAATGTGTTAATTGTAGGTACTGGCCAAGTTGGTATTAGAAG
AGCACGTCGATTTTTAGATGCTGGTGCTTATGTTTCTATTATAACACATGGAATTGATTATGATATAAAAGAGGAATTTA
TAAATAAGGGCGCTGTATTCTATAGTGATAATGCTTTAGATGAATTAATTAAAAAATGTGATTTAGTAGTTGTTGCTACT
GATAATATGGAATTAAACAGGGAAATTTCTCTTAAAGCTCATGATAAATTAGTAAATTGTGCTAGTGATATTTCTTTAAG
TAATGTTATTGTTCCATCAACATTTAATATTGGTTCTGTTACTATTTCATTATATACTAATTCAAAAAGTCCTCTTATGG
CCAAACAGTTACGTAAAAAGATACAGAGTATTATAACTGAAACTGATATTTTATTTATAGAATTACAAGAGTATGTTAGA
TATCTACTAAAAAAACATGTTTCATCACAGGATGATAGAAAAGAATTTATGATACATGTTAATGAAGATGAAATTGTGCA
GAAATTAATTGAAGAAGGAAAATTAGATGAGGCTAAAAAATATGTTAAATGCCTCATAGAAAAGATAAACTAA

Upstream 100 bases:

>100_bases
TTTAAAAACAGCAATTAAAAATCTTAGATTAAAAAGTTTATAATTCTATAAACTTTCTATTTATTACTATTTTTTTATGA
ATCCTTGAAGGTATTGTATT

Downstream 100 bases:

>100_bases
ATTTTAATATAAATATTAGTATTATTAAGTAAATAAATAGTATAAGTAATTAATATATGGTAATGAAATTAGATAAATGT
ATTTTAATTAAATATGAGTA

Product: siroheme synthase

Products: sirohydrochlorin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTVTSLFLDMEEKNVLIVGTGQVGIRRARRFLDAGAYVSIITHGIDYDIKEEFINKGAVFYSDNALDELIKKCDLVVVAT
DNMELNREISLKAHDKLVNCASDISLSNVIVPSTFNIGSVTISLYTNSKSPLMAKQLRKKIQSIITETDILFIELQEYVR
YLLKKHVSSQDDRKEFMIHVNEDEIVQKLIEEGKLDEAKKYVKCLIEKIN

Sequences:

>Translated_210_residues
MTVTSLFLDMEEKNVLIVGTGQVGIRRARRFLDAGAYVSIITHGIDYDIKEEFINKGAVFYSDNALDELIKKCDLVVVAT
DNMELNREISLKAHDKLVNCASDISLSNVIVPSTFNIGSVTISLYTNSKSPLMAKQLRKKIQSIITETDILFIELQEYVR
YLLKKHVSSQDDRKEFMIHVNEDEIVQKLIEEGKLDEAKKYVKCLIEKIN
>Mature_209_residues
TVTSLFLDMEEKNVLIVGTGQVGIRRARRFLDAGAYVSIITHGIDYDIKEEFINKGAVFYSDNALDELIKKCDLVVVATD
NMELNREISLKAHDKLVNCASDISLSNVIVPSTFNIGSVTISLYTNSKSPLMAKQLRKKIQSIITETDILFIELQEYVRY
LLKKHVSSQDDRKEFMIHVNEDEIVQKLIEEGKLDEAKKYVKCLIEKIN

Specific function: Multifunctional Enzyme That Catalyze The Sam-Dependent Methylation Of Uroporphyrinogen III At Position C-2 And C-7 To Form Precorrin-2 And Then Position C-12 Or C-18 To Form Trimethylpyrrocorphin 2. It Also Catalyzes The Conversion Of Precorrin-2 Into Sir

COG id: COG1648

COG function: function code H; Siroheme synthase (precorrin-2 oxidase/ferrochelatase domain)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789768, Length=190, Percent_Identity=25.2631578947368, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011128
- InterPro:   IPR016040
- InterPro:   IPR006367 [H]

Pfam domain/function: PF01210 NAD_Gly3P_dh_N [H]

EC number: 1.3.1.76; 4.99.1.4

Molecular weight: Translated: 23905; Mature: 23774

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTSLFLDMEEKNVLIVGTGQVGIRRARRFLDAGAYVSIITHGIDYDIKEEFINKGAVF
CCHHHEEEEECCCCEEEEECCHHHHHHHHHHHCCCHHEEEHHCCCCCHHHHHHHCCCCEE
YSDNALDELIKKCDLVVVATDNMELNREISLKAHDKLVNCASDISLSNVIVPSTFNIGSV
EECHHHHHHHHHCCEEEEEECCCEECCEEEEHHHHHHHHHHHCCCCCCEEECCCCCCCEE
TISLYTNSKSPLMAKQLRKKIQSIITETDILFIELQEYVRYLLKKHVSSQDDRKEFMIHV
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHCCCCCCCCCEEEEE
NEDEIVQKLIEEGKLDEAKKYVKCLIEKIN
CHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVTSLFLDMEEKNVLIVGTGQVGIRRARRFLDAGAYVSIITHGIDYDIKEEFINKGAVF
CHHHEEEEECCCCEEEEECCHHHHHHHHHHHCCCHHEEEHHCCCCCHHHHHHHCCCCEE
YSDNALDELIKKCDLVVVATDNMELNREISLKAHDKLVNCASDISLSNVIVPSTFNIGSV
EECHHHHHHHHHCCEEEEEECCCEECCEEEEHHHHHHHHHHHCCCCCCEEECCCCCCCEE
TISLYTNSKSPLMAKQLRKKIQSIITETDILFIELQEYVRYLLKKHVSSQDDRKEFMIHV
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHCCCCCCCCCEEEEE
NEDEIVQKLIEEGKLDEAKKYVKCLIEKIN
CHHHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Precorrin 2; NAD+

Specific reaction: precorrin-2 + NAD+ = sirohydrochlorin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]