Click here to switch to the map view.

The map label for this gene is prf1

Identifier: 84490151

GI number: 84490151

Start: 1532530

End: 1533765

Strand: Direct

Name: prf1

Synonym: Msp_1363

Alternate gene names: 84490151

Gene position: 1532530-1533765 (Clockwise)

Preceding gene: 84490148

Following gene: 84490166

Centisome position: 86.71

GC content: 32.36

Gene sequence:

>1236_bases
ATGAGTGATGTATCATCAAAAGAAATATATGAAGTAAAAAAGACTCTTAAAGAGTTAGAAGATAAAAAAGGTAGAGGAAC
AGAACTTGTAAGTGTTTATATACCACCTGAAAAACAGATAAGTGATGTTGCAAAACAGATGCGTGATGAACTTGGACAAA
GTGCAAACATTAAAAGTAAACAAACAAGAAAAAATGTACAATCAGCTATTGAAGTAATTATTCAAAGATTAAAATTATTC
CCAAAACCACCAGAAAAGGGACTTGTAATGTTTGTGGGAATGATTCCAAAAGGTGGACCTGGTACTGAGAAAATGGAAAC
ATATGTATTCCAACCACCTGAAGCTGTTCAAACATACACATACCACTGTGATAGTCAGTTTTTTGTAGAACCTCTAAAAC
AAATTATTGAATATAAGGAAGTTTATGGTGTAGTAGTACTAGATCGTAAAGAATCTACAATTGCAACACTAAGAGGAAAA
AGAATAGATATTATAAAACACTTAACTAGTGGTGTTCCAGGAAAACATAAAGCTGGAGGACAGTCACAAAGAAGATTTGA
CCGTGTGATTGAATTAGCTGCACATGAATTCCTTAAACGTATAGGAAGACACGTAGATGAAGCATTTCTTCCATTAAAAG
ATGAACTTAAGGGTGTTCTTATTGGTGGACCAGGCCATACTAAAAATGATTTTGTTGATGGAGAGTATATACACTATGAA
ATTCATGATAAAATAATCAACATTGTTGACACATCATACACTGGAGATTTTGGTATAAGAGAAGTTATTGATGAATCTGC
AGATACACTTGATGAAATGGATATTATGCAAGAGAAGAAGTTCATGAGAAAATTCCTTACAGGACTTATATCTGAAAGTG
GACTTTCAACTTATGGTGAAAAAGAAGTAAGACAAAATCTTCAAATGGGTGCTGTTGAAACATTACTCATATCTGAAAAT
CTTAAAAGTAAAAGACAAACCTACACTTGTCCTGCATGTAATACAGTAGATGTTATAACTACTAGACAACATCAAGAACC
TCCAGAAAAAAGATGTCCTAAATGTAATGAAGTTATGAAAATAACTAAAACACAAGAAACTGCTGAAGAATTAATAGAAT
TGGCCGAAGAAGTTAAAACACATGTTGAAGTTATTTCTATAGAAACCGAAGAAGGAACTCAACTTGATAAGGCATTTGGT
GGAATTGCTGGTATTTTAAGATATAAAGTAAAATAG

Upstream 100 bases:

>100_bases
AAATGACTCCCTCCCATATTTAATATATTCTTATTAATCCCAGATAATTATTCTAATCAATATTAATTAAATCACAACAT
TAATCAAAGAAGAGGATAAA

Downstream 100 bases:

>100_bases
ATTTACTTTATTCTATTTTTTACAATTTTTTTTAATGAATCTTTATGAAATTCATATTTATAAGATACTTTTTTATATTA
AAACCTAAATTTTTCTATAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: Translation termination factor aRF1

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSDVSSKEIYEVKKTLKELEDKKGRGTELVSVYIPPEKQISDVAKQMRDELGQSANIKSKQTRKNVQSAIEVIIQRLKLF
PKPPEKGLVMFVGMIPKGGPGTEKMETYVFQPPEAVQTYTYHCDSQFFVEPLKQIIEYKEVYGVVVLDRKESTIATLRGK
RIDIIKHLTSGVPGKHKAGGQSQRRFDRVIELAAHEFLKRIGRHVDEAFLPLKDELKGVLIGGPGHTKNDFVDGEYIHYE
IHDKIINIVDTSYTGDFGIREVIDESADTLDEMDIMQEKKFMRKFLTGLISESGLSTYGEKEVRQNLQMGAVETLLISEN
LKSKRQTYTCPACNTVDVITTRQHQEPPEKRCPKCNEVMKITKTQETAEELIELAEEVKTHVEVISIETEEGTQLDKAFG
GIAGILRYKVK

Sequences:

>Translated_411_residues
MSDVSSKEIYEVKKTLKELEDKKGRGTELVSVYIPPEKQISDVAKQMRDELGQSANIKSKQTRKNVQSAIEVIIQRLKLF
PKPPEKGLVMFVGMIPKGGPGTEKMETYVFQPPEAVQTYTYHCDSQFFVEPLKQIIEYKEVYGVVVLDRKESTIATLRGK
RIDIIKHLTSGVPGKHKAGGQSQRRFDRVIELAAHEFLKRIGRHVDEAFLPLKDELKGVLIGGPGHTKNDFVDGEYIHYE
IHDKIINIVDTSYTGDFGIREVIDESADTLDEMDIMQEKKFMRKFLTGLISESGLSTYGEKEVRQNLQMGAVETLLISEN
LKSKRQTYTCPACNTVDVITTRQHQEPPEKRCPKCNEVMKITKTQETAEELIELAEEVKTHVEVISIETEEGTQLDKAFG
GIAGILRYKVK
>Mature_410_residues
SDVSSKEIYEVKKTLKELEDKKGRGTELVSVYIPPEKQISDVAKQMRDELGQSANIKSKQTRKNVQSAIEVIIQRLKLFP
KPPEKGLVMFVGMIPKGGPGTEKMETYVFQPPEAVQTYTYHCDSQFFVEPLKQIIEYKEVYGVVVLDRKESTIATLRGKR
IDIIKHLTSGVPGKHKAGGQSQRRFDRVIELAAHEFLKRIGRHVDEAFLPLKDELKGVLIGGPGHTKNDFVDGEYIHYEI
HDKIINIVDTSYTGDFGIREVIDESADTLDEMDIMQEKKFMRKFLTGLISESGLSTYGEKEVRQNLQMGAVETLLISENL
KSKRQTYTCPACNTVDVITTRQHQEPPEKRCPKCNEVMKITKTQETAEELIELAEEVKTHVEVISIETEEGTQLDKAFGG
IAGILRYKVK

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family

Homologues:

Organism=Homo sapiens, GI4759034, Length=406, Percent_Identity=30.5418719211823, Blast_Score=219, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI72000368, Length=407, Percent_Identity=29.97542997543, Blast_Score=211, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6319619, Length=405, Percent_Identity=31.1111111111111, Blast_Score=211, Evalue=1e-55,
Organism=Drosophila melanogaster, GI28574632, Length=407, Percent_Identity=31.2039312039312, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI21356433, Length=407, Percent_Identity=31.2039312039312, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24667392, Length=407, Percent_Identity=31.2039312039312, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24667388, Length=407, Percent_Identity=31.2039312039312, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24667380, Length=407, Percent_Identity=31.2039312039312, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24667384, Length=407, Percent_Identity=31.2039312039312, Blast_Score=224, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF1_METST (Q2NEL3)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_448383.1
- ProteinModelPortal:   Q2NEL3
- SMR:   Q2NEL3
- STRING:   Q2NEL3
- GeneID:   3855102
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_1363
- NMPDR:   fig|339860.6.peg.1308
- eggNOG:   arNOG04578
- HOGENOM:   HBG497188
- OMA:   GDYLHHE
- PhylomeDB:   Q2NEL3
- ProtClustDB:   PRK04011
- BioCyc:   MSTA339860:MSP_1363-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00424
- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403
- PANTHER:   PTHR10113
- PANTHER:   PTHR10113:SF2
- TIGRFAMs:   TIGR03676

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3

EC number: NA

Molecular weight: Translated: 46550; Mature: 46419

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVSSKEIYEVKKTLKELEDKKGRGTELVSVYIPPEKQISDVAKQMRDELGQSANIKSK
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHH
QTRKNVQSAIEVIIQRLKLFPKPPEKGLVMFVGMIPKGGPGTEKMETYVFQPPEAVQTYT
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHEEECCCHHHHHEE
YHCDSQFFVEPLKQIIEYKEVYGVVVLDRKESTIATLRGKRIDIIKHLTSGVPGKHKAGG
EECCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCC
QSQRRFDRVIELAAHEFLKRIGRHVDEAFLPLKDELKGVLIGGPGHTKNDFVDGEYIHYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEECCCCCCCCCCCCCCEEEEE
IHDKIINIVDTSYTGDFGIREVIDESADTLDEMDIMQEKKFMRKFLTGLISESGLSTYGE
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
KEVRQNLQMGAVETLLISENLKSKRQTYTCPACNTVDVITTRQHQEPPEKRCPKCNEVMK
HHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCEEEEECCCCCCCCHHHCCCHHHHHH
ITKTQETAEELIELAEEVKTHVEVISIETEEGTQLDKAFGGIAGILRYKVK
HHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDVSSKEIYEVKKTLKELEDKKGRGTELVSVYIPPEKQISDVAKQMRDELGQSANIKSK
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHH
QTRKNVQSAIEVIIQRLKLFPKPPEKGLVMFVGMIPKGGPGTEKMETYVFQPPEAVQTYT
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHEEECCCHHHHHEE
YHCDSQFFVEPLKQIIEYKEVYGVVVLDRKESTIATLRGKRIDIIKHLTSGVPGKHKAGG
EECCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCC
QSQRRFDRVIELAAHEFLKRIGRHVDEAFLPLKDELKGVLIGGPGHTKNDFVDGEYIHYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEECCCCCCCCCCCCCCEEEEE
IHDKIINIVDTSYTGDFGIREVIDESADTLDEMDIMQEKKFMRKFLTGLISESGLSTYGE
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
KEVRQNLQMGAVETLLISENLKSKRQTYTCPACNTVDVITTRQHQEPPEKRCPKCNEVMK
HHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCEEEEECCCCCCCCHHHCCCHHHHHH
ITKTQETAEELIELAEEVKTHVEVISIETEEGTQLDKAFGGIAGILRYKVK
HHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA