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The map label for this gene is tfb

Identifier: 84490148

GI number: 84490148

Start: 1530147

End: 1531082

Strand: Direct

Name: tfb

Synonym: Msp_1360

Alternate gene names: 84490148

Gene position: 1530147-1531082 (Clockwise)

Preceding gene: 84490147

Following gene: 84490151

Centisome position: 86.58

GC content: 36.0

Gene sequence:

>936_bases
ATGAAAGAAGACGTTGCTGAAATGGAGAAAAAAGAAACTAAATGTCCAGAATGTGGTTCTACAAAATTAATAAATGATCA
TGAACGTGGAGAAGTTGTTTGTGGAGCATGTGGTCTTGTAATAGATGATAACATAGTGGATATGGGTCCTGAATGGAGAG
CTTTTGACCATGAACAAAGAGATAAAAGAACAAGAGTAGGAGCACCTATCACATACACAATTCACGATAAAGGTTTATCC
ACCATGATTGATTGGCGTAACAAAGACATCTATGGAAGAGATATACCTGCAAGAAACAGAGCTCAATGGTACAGACTAAG
AAAATGGCAGAGAAAAATCAGAATCAGTGGTGCTACAGAAAGAAACCTTGCATTTGCATTAAGTGAACTTGATAGAGATT
CTTCAAGATTAGGTCTTCCAAGATCTGTACGTGAATCAGCATCAGTTGTATATAGAAATGCTGTTGAAAATAAACTCATA
CGTGGAAGAAGTATTGAAGGAGTTGTAGCAGCTTCATTATATGCTGCATGTAGAAGATGTAAAGTTCCAAGAACACTTGA
TGAAATAGCTGATGTATCTCGTGTAAGTAAGAAAGAAGTTGGTAGAACATACAGATTCCTTACAAGAGAGTTACATATTA
GATTACCACCAACATCCCCTATTGATTATGTCCCTAGATTTGCTAGTGAATTAAATCTATCAGGTGTTGTTCAGTCTAAA
GCTATTGAAATTATTAATCAAGCAATGGATAATGGTCTTACAAGTGGAAGAGGACCTACTGGTGTTGCAGCTGCTGCATT
ATACATTGCAAGTGTGTTACTTGGTGAACGTAAAACACAACGTGATGTTGCAGATATTGCTGGTGTAACAGAAGTAACAA
TCAGAAACAGATACAAAGAATTAACAGAACAATTAGATATGGGTGTGAATTTATAA

Upstream 100 bases:

>100_bases
TGATTTAGATGTTGAAGAAATTATAAAAATAGATACAACAGACTCTGAAGAACTAGAACATGAATTAGAAAAAAGCAACG
GCATAAGTGAAAAAGAACAA

Downstream 100 bases:

>100_bases
GGTTCACATCAATTCTTTTTTTATATAATTTCAACTATTTTTTAAAATATTAATTTAAAATAAGCATTTTTTCTAAAAAG
AATAATAAAAAAAATAGAAA

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKEDVAEMEKKETKCPECGSTKLINDHERGEVVCGACGLVIDDNIVDMGPEWRAFDHEQRDKRTRVGAPITYTIHDKGLS
TMIDWRNKDIYGRDIPARNRAQWYRLRKWQRKIRISGATERNLAFALSELDRDSSRLGLPRSVRESASVVYRNAVENKLI
RGRSIEGVVAASLYAACRRCKVPRTLDEIADVSRVSKKEVGRTYRFLTRELHIRLPPTSPIDYVPRFASELNLSGVVQSK
AIEIINQAMDNGLTSGRGPTGVAAAALYIASVLLGERKTQRDVADIAGVTEVTIRNRYKELTEQLDMGVNL

Sequences:

>Translated_311_residues
MKEDVAEMEKKETKCPECGSTKLINDHERGEVVCGACGLVIDDNIVDMGPEWRAFDHEQRDKRTRVGAPITYTIHDKGLS
TMIDWRNKDIYGRDIPARNRAQWYRLRKWQRKIRISGATERNLAFALSELDRDSSRLGLPRSVRESASVVYRNAVENKLI
RGRSIEGVVAASLYAACRRCKVPRTLDEIADVSRVSKKEVGRTYRFLTRELHIRLPPTSPIDYVPRFASELNLSGVVQSK
AIEIINQAMDNGLTSGRGPTGVAAAALYIASVLLGERKTQRDVADIAGVTEVTIRNRYKELTEQLDMGVNL
>Mature_311_residues
MKEDVAEMEKKETKCPECGSTKLINDHERGEVVCGACGLVIDDNIVDMGPEWRAFDHEQRDKRTRVGAPITYTIHDKGLS
TMIDWRNKDIYGRDIPARNRAQWYRLRKWQRKIRISGATERNLAFALSELDRDSSRLGLPRSVRESASVVYRNAVENKLI
RGRSIEGVVAASLYAACRRCKVPRTLDEIADVSRVSKKEVGRTYRFLTRELHIRLPPTSPIDYVPRFASELNLSGVVQSK
AIEIINQAMDNGLTSGRGPTGVAAAALYIASVLLGERKTQRDVADIAGVTEVTIRNRYKELTEQLDMGVNL

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB)

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger

Homologues:

Organism=Homo sapiens, GI4504193, Length=286, Percent_Identity=35.3146853146853, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI22035556, Length=290, Percent_Identity=28.2758620689655, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17564892, Length=289, Percent_Identity=38.7543252595156, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17531397, Length=292, Percent_Identity=26.027397260274, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6325343, Length=308, Percent_Identity=29.2207792207792, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321685, Length=294, Percent_Identity=24.8299319727891, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136758, Length=282, Percent_Identity=34.0425531914894, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI281361961, Length=303, Percent_Identity=27.3927392739274, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24647658, Length=303, Percent_Identity=27.3927392739274, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TF2B_METST (Q2NEL6)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_448380.1
- ProteinModelPortal:   Q2NEL6
- STRING:   Q2NEL6
- GeneID:   3855099
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_1360
- NMPDR:   fig|339860.6.peg.1305
- eggNOG:   arNOG04485
- HOGENOM:   HBG646536
- OMA:   HDYERAE
- PhylomeDB:   Q2NEL6
- ProtClustDB:   PRK00423
- BioCyc:   MSTA339860:MSP_1360-MONOMER
- HAMAP:   MF_00383
- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137
- Gene3D:   G3DSA:1.10.472.10
- PANTHER:   PTHR11618
- PRINTS:   PR00685
- SMART:   SM00385

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon; SSF47954 Cyclin_like

EC number: NA

Molecular weight: Translated: 35016; Mature: 35016

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00782 TFIIB; PS51134 ZF_TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEDVAEMEKKETKCPECGSTKLINDHERGEVVCGACGLVIDDNIVDMGPEWRAFDHEQR
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCEEEECCEECCCCCCCCCCCHHH
DKRTRVGAPITYTIHDKGLSTMIDWRNKDIYGRDIPARNRAQWYRLRKWQRKIRISGATE
HHHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCH
RNLAFALSELDRDSSRLGLPRSVRESASVVYRNAVENKLIRGRSIEGVVAASLYAACRRC
HHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
KVPRTLDEIADVSRVSKKEVGRTYRFLTRELHIRLPPTSPIDYVPRFASELNLSGVVQSK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCHHHHHHHH
AIEIINQAMDNGLTSGRGPTGVAAAALYIASVLLGERKTQRDVADIAGVTEVTIRNRYKE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHH
LTEQLDMGVNL
HHHHHHCCCCC
>Mature Secondary Structure
MKEDVAEMEKKETKCPECGSTKLINDHERGEVVCGACGLVIDDNIVDMGPEWRAFDHEQR
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCEEEECCEECCCCCCCCCCCHHH
DKRTRVGAPITYTIHDKGLSTMIDWRNKDIYGRDIPARNRAQWYRLRKWQRKIRISGATE
HHHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCH
RNLAFALSELDRDSSRLGLPRSVRESASVVYRNAVENKLIRGRSIEGVVAASLYAACRRC
HHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
KVPRTLDEIADVSRVSKKEVGRTYRFLTRELHIRLPPTSPIDYVPRFASELNLSGVVQSK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCHHHHHHHH
AIEIINQAMDNGLTSGRGPTGVAAAALYIASVLLGERKTQRDVADIAGVTEVTIRNRYKE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHH
LTEQLDMGVNL
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA