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The map label for this gene is pyrE [C]

Identifier: 84490122

GI number: 84490122

Start: 1502079

End: 1502675

Strand: Direct

Name: pyrE [C]

Synonym: Msp_1334

Alternate gene names: 84490122

Gene position: 1502079-1502675 (Clockwise)

Preceding gene: 84490121

Following gene: 84490123

Centisome position: 84.99

GC content: 29.82

Gene sequence:

>597_bases
ATGAAAAATAAATTAATTGAAAAAGCTACTGAACTTAGAAATAAGGGATTAACCACTGGTGAAATTGCAGATGAATTAAA
TATATCTAAAGATACAACTCAATGGTTAATTATGCAAATGACCACAGTTAATAAAACAAAACAGAAACAAAAACCTGATG
ATTTTGCTATTAATTGGAAAACTATTGGTTCTAGTTCATCACGTATGCAGTATATTGCTTCAGCATTATCTGACATGGCT
GTTGAAGAAGGAGTTGTTGATGCAGTTGTAGGTATTAGTATTAGTGGAGTACCATTTGCTACTATAATGGCTGAAATATT
AGATGCTGAACTTGCAGTATTTCATCCAATTAAACATATGAAAAATGAATCAGCACAAGGAGCTCTAAGTCATAACTTTG
CTAATATTAAAAATAAAACAGTTGTAATTGTAGATGATGTTATAACAAGTGGAGCTACAATTACTGATGCTATTAGAGTA
TGTAAAAAAAATGGTGCAAATCCTCTAGTTGTCACAGTTCTTGTGGATAAAAAAGGACTTGATGATTCTGATAATGTACC
TATTAAATCATTGATTAAAATTAATAAAGTAGGATAA

Upstream 100 bases:

>100_bases
GTATCTGGTAAAGATGGATATGAAGCATTGAGAATTGTTGATGCTGCAATGACCTCATCCAAAGATAAAAGACTAGTGTA
TTTAGATTAAAGTGATGAAA

Downstream 100 bases:

>100_bases
AAATAATTTCTTATTTCTCTTTTTTCTTTAAATAATACCATAATAGATTTAATTAAAAAAAATACATTAATAATCACAAT
ACATTAAAAATAATATATAT

Product: orotate phosphoribosyltransferase-like protein

Products: orotate; 5-phospho-alpha-D-ribose 1-diphosphate

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MKNKLIEKATELRNKGLTTGEIADELNISKDTTQWLIMQMTTVNKTKQKQKPDDFAINWKTIGSSSSRMQYIASALSDMA
VEEGVVDAVVGISISGVPFATIMAEILDAELAVFHPIKHMKNESAQGALSHNFANIKNKTVVIVDDVITSGATITDAIRV
CKKNGANPLVVTVLVDKKGLDDSDNVPIKSLIKINKVG

Sequences:

>Translated_198_residues
MKNKLIEKATELRNKGLTTGEIADELNISKDTTQWLIMQMTTVNKTKQKQKPDDFAINWKTIGSSSSRMQYIASALSDMA
VEEGVVDAVVGISISGVPFATIMAEILDAELAVFHPIKHMKNESAQGALSHNFANIKNKTVVIVDDVITSGATITDAIRV
CKKNGANPLVVTVLVDKKGLDDSDNVPIKSLIKINKVG
>Mature_198_residues
MKNKLIEKATELRNKGLTTGEIADELNISKDTTQWLIMQMTTVNKTKQKQKPDDFAINWKTIGSSSSRMQYIASALSDMA
VEEGVVDAVVGISISGVPFATIMAEILDAELAVFHPIKHMKNESAQGALSHNFANIKNKTVVIVDDVITSGATITDAIRV
CKKNGANPLVVTVLVDKKGLDDSDNVPIKSLIKINKVG

Specific function: Pyrimidine biosynthesis; fifth step. [C]

COG id: COG0856

COG function: function code F; Orotate phosphoribosyltransferase homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYREL_METST (Q2NEP2)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_448354.1
- ProteinModelPortal:   Q2NEP2
- STRING:   Q2NEP2
- GeneID:   3855657
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_1334
- NMPDR:   fig|339860.6.peg.1280
- eggNOG:   arNOG07847
- HOGENOM:   HBG497993
- OMA:   KDVHIDW
- ProtClustDB:   PRK02277
- BioCyc:   MSTA339860:MSP_1334-MONOMER
- HAMAP:   MF_01214
- InterPro:   IPR022854
- InterPro:   IPR000836

Pfam domain/function: PF00156 Pribosyltran

EC number: 2.4.2.10

Molecular weight: Translated: 21436; Mature: 21436

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKLIEKATELRNKGLTTGEIADELNISKDTTQWLIMQMTTVNKTKQKQKPDDFAINWK
CCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHEEEEHHHHCCHHHHHCCCCCEEEEEE
TIGSSSSRMQYIASALSDMAVEEGVVDAVVGISISGVPFATIMAEILDAELAVFHPIKHM
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHHH
KNESAQGALSHNFANIKNKTVVIVDDVITSGATITDAIRVCKKNGANPLVVTVLVDKKGL
CCCCCCCHHHHCHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCC
DDSDNVPIKSLIKINKVG
CCCCCCCHHHHHEECCCC
>Mature Secondary Structure
MKNKLIEKATELRNKGLTTGEIADELNISKDTTQWLIMQMTTVNKTKQKQKPDDFAINWK
CCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHEEEEHHHHCCHHHHHCCCCCEEEEEE
TIGSSSSRMQYIASALSDMAVEEGVVDAVVGISISGVPFATIMAEILDAELAVFHPIKHM
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHHH
KNESAQGALSHNFANIKNKTVVIVDDVITSGATITDAIRVCKKNGANPLVVTVLVDKKGL
CCCCCCCHHHHCHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCC
DDSDNVPIKSLIKINKVG
CCCCCCCHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ba2+; Ca2+; Co2+; Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.22 {diphosphate}} 0.013 {diphosphate}} 0.0031 {orotidine} 0.44 {orotate}} 0.03 {orotate}} 0.36 {phosphoribose} 0.038 {phosphoribose} [C]

Substrates: orotidine 5'-phosphate; diphosphate

Specific reaction: orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate

General reaction: Glycosyl and Pentosyl group transfer [C]

Inhibitor: HgCl2; IAA; NEM; PCMB; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA