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The map label for this gene is ppnK

Identifier: 84490118

GI number: 84490118

Start: 1497804

End: 1498631

Strand: Direct

Name: ppnK

Synonym: Msp_1330

Alternate gene names: 84490118

Gene position: 1497804-1498631 (Clockwise)

Preceding gene: 84490117

Following gene: 84490119

Centisome position: 84.75

GC content: 29.71

Gene sequence:

>828_bases
ATGAGAATTGGAATTATCTCACGTACTGATAAAGAAGAAGCTATAGAACTTGATGATACTATTATAAAATATTTATTTGA
AAATAATATTGAAGTTGAATTAGATTCACAACTAACTAAAAAACTTCCACAATACTCAGAAAATAGTGTAGATATTACAA
AGATGAATGCTGATATTGTATTATGTGTTGGTGGTGATGGAACAGTCCTACATGCTCAACGTTATCTATCTCCAAAGAAA
ATACCAATACTTAGTATTAATATGGGAACTGTGGGTTTTTTAACTGAAGTTGATCCTGAGGACATATTTGAATGTCTTGA
TAAATTATTAAGTTATGATTTCTTTATAGAAGAAAGATTACAATTAGATGTTCTAATTGATTCACAATGGCATACTGTTT
TAAATGAATTGGTGTTAATGACAAGTCAACCTGCTAAAATGTTAGATTTACGTGTTTCTGTGGATGAAGAAATTGTAGAT
GAAGTTCGTGCTGATGGCTTAATTATATCCACACCCAGTGGATCTACTGCTTATGCTATGTCTGCGGGAGGACCAATTGT
TGATCCTAGGGTTGATGCAGCAATTATCATACCAATATGTCCATTTAAATTAAATACTAGGCCAAAAATAGTTCCAGCAG
ATAGTATCATCACAGTTAAATTCTTAAAAGAAGGTAAAAAAGGAGTTGCTGTTCTTGATGGTATTGTAAATAAGGAATTT
GACTATTTAGGAGAAATTAAACTTAAAAAATCCGAAAATTCAGCATATTTTGTTAGATTTAAAAAGAATTTCTATAATAG
TGTAAATAATAAATTAATTGTAGGTTAA

Upstream 100 bases:

>100_bases
AATCAATTGAATTTAAAAGAAAAAACATCGATAATAGCAGCAGCAAATGAGAAAATACATGAAGATATTATTAAATTGTT
AGAATAAGTTTGTGATTTAT

Downstream 100 bases:

>100_bases
CTACAATTTTCTCTTTTTTAGGTGTTGATATGAAAAACATATTAATTAGTGATGCTAATCATGGTGGACTTACACTTCTT
GAAGAATATTCAAAATACAC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRIGIISRTDKEEAIELDDTIIKYLFENNIEVELDSQLTKKLPQYSENSVDITKMNADIVLCVGGDGTVLHAQRYLSPKK
IPILSINMGTVGFLTEVDPEDIFECLDKLLSYDFFIEERLQLDVLIDSQWHTVLNELVLMTSQPAKMLDLRVSVDEEIVD
EVRADGLIISTPSGSTAYAMSAGGPIVDPRVDAAIIIPICPFKLNTRPKIVPADSIITVKFLKEGKKGVAVLDGIVNKEF
DYLGEIKLKKSENSAYFVRFKKNFYNSVNNKLIVG

Sequences:

>Translated_275_residues
MRIGIISRTDKEEAIELDDTIIKYLFENNIEVELDSQLTKKLPQYSENSVDITKMNADIVLCVGGDGTVLHAQRYLSPKK
IPILSINMGTVGFLTEVDPEDIFECLDKLLSYDFFIEERLQLDVLIDSQWHTVLNELVLMTSQPAKMLDLRVSVDEEIVD
EVRADGLIISTPSGSTAYAMSAGGPIVDPRVDAAIIIPICPFKLNTRPKIVPADSIITVKFLKEGKKGVAVLDGIVNKEF
DYLGEIKLKKSENSAYFVRFKKNFYNSVNNKLIVG
>Mature_275_residues
MRIGIISRTDKEEAIELDDTIIKYLFENNIEVELDSQLTKKLPQYSENSVDITKMNADIVLCVGGDGTVLHAQRYLSPKK
IPILSINMGTVGFLTEVDPEDIFECLDKLLSYDFFIEERLQLDVLIDSQWHTVLNELVLMTSQPAKMLDLRVSVDEEIVD
EVRADGLIISTPSGSTAYAMSAGGPIVDPRVDAAIIIPICPFKLNTRPKIVPADSIITVKFLKEGKKGVAVLDGIVNKEF
DYLGEIKLKKSENSAYFVRFKKNFYNSVNNKLIVG

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=244, Percent_Identity=27.8688524590164, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1788968, Length=226, Percent_Identity=34.070796460177, Blast_Score=141, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6325068, Length=203, Percent_Identity=33.0049261083744, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322509, Length=199, Percent_Identity=33.6683417085427, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320794, Length=176, Percent_Identity=35.2272727272727, Blast_Score=102, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28573830, Length=235, Percent_Identity=27.6595744680851, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28573826, Length=235, Percent_Identity=27.6595744680851, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161077047, Length=235, Percent_Identity=27.6595744680851, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28573828, Length=235, Percent_Identity=27.6595744680851, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28573832, Length=235, Percent_Identity=27.6595744680851, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129957, Length=263, Percent_Identity=25.0950570342205, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24653424, Length=263, Percent_Identity=25.0950570342205, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI281363323, Length=263, Percent_Identity=25.0950570342205, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24653422, Length=263, Percent_Identity=25.0950570342205, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281363321, Length=263, Percent_Identity=25.0950570342205, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_METST (Q2NEP6)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_448350.1
- HSSP:   O30297
- ProteinModelPortal:   Q2NEP6
- SMR:   Q2NEP6
- STRING:   Q2NEP6
- GeneID:   3855653
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_1330
- NMPDR:   fig|339860.6.peg.1276
- eggNOG:   arNOG04399
- HOGENOM:   HBG713904
- OMA:   FLVEVEP
- PhylomeDB:   Q2NEP6
- ProtClustDB:   PRK03708
- BioCyc:   MSTA339860:MSP_1330-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 30748; Mature: 30748

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIISRTDKEEAIELDDTIIKYLFENNIEVELDSQLTKKLPQYSENSVDITKMNADIV
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCCEEEEEECCCEE
LCVGGDGTVLHAQRYLSPKKIPILSINMGTVGFLTEVDPEDIFECLDKLLSYDFFIEERL
EEECCCCCEEEHHHHCCCCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHEEECCE
QLDVLIDSQWHTVLNELVLMTSQPAKMLDLRVSVDEEIVDEVRADGLIISTPSGSTAYAM
EEEEEECCHHHHHHHHHHHHCCCCCHHEEEEECCCHHHHHHHHHCCEEEECCCCCEEEEE
SAGGPIVDPRVDAAIIIPICPFKLNTRPKIVPADSIITVKFLKEGKKGVAVLDGIVNKEF
ECCCCCCCCCCCEEEEEEECCCEECCCCCEEECCCEEEEEEECCCCCCEEEHHHHHCCCC
DYLGEIKLKKSENSAYFVRFKKNFYNSVNNKLIVG
CCCEEEEEEECCCCEEEEEEEHHHHHCCCCEEEEC
>Mature Secondary Structure
MRIGIISRTDKEEAIELDDTIIKYLFENNIEVELDSQLTKKLPQYSENSVDITKMNADIV
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCCEEEEEECCCEE
LCVGGDGTVLHAQRYLSPKKIPILSINMGTVGFLTEVDPEDIFECLDKLLSYDFFIEERL
EEECCCCCEEEHHHHCCCCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHEEECCE
QLDVLIDSQWHTVLNELVLMTSQPAKMLDLRVSVDEEIVDEVRADGLIISTPSGSTAYAM
EEEEEECCHHHHHHHHHHHHCCCCCHHEEEEECCCHHHHHHHHHCCEEEECCCCCEEEEE
SAGGPIVDPRVDAAIIIPICPFKLNTRPKIVPADSIITVKFLKEGKKGVAVLDGIVNKEF
ECCCCCCCCCCCEEEEEEECCCEECCCCCEEECCCEEEEEEECCCCCCEEEHHHHHCCCC
DYLGEIKLKKSENSAYFVRFKKNFYNSVNNKLIVG
CCCEEEEEEECCCCEEEEEEEHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA