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The map label for this gene is frhA

Identifier: 84490090

GI number: 84490090

Start: 1467211

End: 1468431

Strand: Direct

Name: frhA

Synonym: Msp_1302

Alternate gene names: 84490090

Gene position: 1467211-1468431 (Clockwise)

Preceding gene: 84490089

Following gene: 84490091

Centisome position: 83.02

GC content: 33.42

Gene sequence:

>1221_bases
TTGAGTGAAACTATAGTAATATCCCCGACAAGTCGTCAAGAGGGACATGCAGAATTATCAATGGAAGTTGATGAGAACGG
AATAGTAACAACAGGACGTTACTTTAGTATTACACCTGTACGAGGAATAGAAAAAATGGTTATTGGAAAAAGACCAGAAA
CTGCTCCAGTACTTGTTCAAAGAATATGTGGAGTATGTCCAGTAACACATACATTAGCATCAGTTGAAGCAATTGATGAT
TCCCTAAAAATAGAAGTACCCCAAGCAGGACGTATTTTAAGAGAAATGTGTCTTAATGCACATATGATAAATAGTCATGC
AATACACCACTTTTTAGTAGCTCCCGACTTTGTACCAGATAACCAGTATAAAGAAGTTGTTAAAAACATATCAACAATAA
GAAAAAATGCTCAATATGTTGAAGATACAGTTGGAGGAGAAGGAATTCATCCATCTGATATTAGAATTGGTGGTATGGCA
CATAACATAACAAAAAATACAAAAAATAAAATTAAAACAAGAATCAGTGGGATGATGAAACTTCTAGAAGAACATGTGGA
AACAATGGCTGGACTAATAGAAGATAAAGATTTCCCAGACAAACTAGGAGCACATAATCAACCTGTATTTGCTTCAGATA
ATACATATGGTTCAAAAAATAATCTTTCTATTAATGAAATAGAAGAAATATTACCAAGTTCTTGGTATGATCTTCCAGAT
ATTGGAGAAAGAGCATGTTCACAAATTCCATTATATAAAGGAAATGTTGTAGAAACAGGACCTAGAGCAAGAGCATCAAA
ATTCCGTAAATTTAAAGAAGTAGGAGTTAAAGCACAACATCTTGCACGTGCACAAGAAATGATAAATTCTGCTGAAAAAA
TACTTGAATTAGTTGATAAACTTGATACATCTGCACAAGTAATGGCAGATTACACATTAGAAGGAACAAATCGTTTAGGT
GTAGGAGTAATTGAAGGTCCAAGAGGAACAAATATTCATTCAGCAAAAATATCTCCAGAAGGATACATCAGTTATTATAA
TGCAATTGTACCTACAACATGGAATATTCCAACAATGGGACTTGCTACTGAAGGATTCCATCATGAATATGCTCCACATG
TAATAAGAGCATACGACCCATGTTTATCATGTGCTACACATATGATTGTTAAAGATGATGAAACTAAAGAAGTATTAAAG
GATGATATGGTTCAATTATAA

Upstream 100 bases:

>100_bases
TATATTCAAAATAAAATAGAATTAAAGGCTATTTTTTAATATAAAAATAGAAGAAAATGTTGTTTCATATTTAATTAATA
AAGAGAATAGGTGATTTAGT

Downstream 100 bases:

>100_bases
TTTGAAGGTATATTATGTCATATAATCATGAAAATTTAATAGTAGGCTGTGGAAATATTCTCTTTGGAGACGATGGTTTT
GGTCCAAAGGTTATTGAATA

Product: FrhA

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit alpha; FRH [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSETIVISPTSRQEGHAELSMEVDENGIVTTGRYFSITPVRGIEKMVIGKRPETAPVLVQRICGVCPVTHTLASVEAIDD
SLKIEVPQAGRILREMCLNAHMINSHAIHHFLVAPDFVPDNQYKEVVKNISTIRKNAQYVEDTVGGEGIHPSDIRIGGMA
HNITKNTKNKIKTRISGMMKLLEEHVETMAGLIEDKDFPDKLGAHNQPVFASDNTYGSKNNLSINEIEEILPSSWYDLPD
IGERACSQIPLYKGNVVETGPRARASKFRKFKEVGVKAQHLARAQEMINSAEKILELVDKLDTSAQVMADYTLEGTNRLG
VGVIEGPRGTNIHSAKISPEGYISYYNAIVPTTWNIPTMGLATEGFHHEYAPHVIRAYDPCLSCATHMIVKDDETKEVLK
DDMVQL

Sequences:

>Translated_406_residues
MSETIVISPTSRQEGHAELSMEVDENGIVTTGRYFSITPVRGIEKMVIGKRPETAPVLVQRICGVCPVTHTLASVEAIDD
SLKIEVPQAGRILREMCLNAHMINSHAIHHFLVAPDFVPDNQYKEVVKNISTIRKNAQYVEDTVGGEGIHPSDIRIGGMA
HNITKNTKNKIKTRISGMMKLLEEHVETMAGLIEDKDFPDKLGAHNQPVFASDNTYGSKNNLSINEIEEILPSSWYDLPD
IGERACSQIPLYKGNVVETGPRARASKFRKFKEVGVKAQHLARAQEMINSAEKILELVDKLDTSAQVMADYTLEGTNRLG
VGVIEGPRGTNIHSAKISPEGYISYYNAIVPTTWNIPTMGLATEGFHHEYAPHVIRAYDPCLSCATHMIVKDDETKEVLK
DDMVQL
>Mature_405_residues
SETIVISPTSRQEGHAELSMEVDENGIVTTGRYFSITPVRGIEKMVIGKRPETAPVLVQRICGVCPVTHTLASVEAIDDS
LKIEVPQAGRILREMCLNAHMINSHAIHHFLVAPDFVPDNQYKEVVKNISTIRKNAQYVEDTVGGEGIHPSDIRIGGMAH
NITKNTKNKIKTRISGMMKLLEEHVETMAGLIEDKDFPDKLGAHNQPVFASDNTYGSKNNLSINEIEEILPSSWYDLPDI
GERACSQIPLYKGNVVETGPRARASKFRKFKEVGVKAQHLARAQEMINSAEKILELVDKLDTSAQVMADYTLEGTNRLGV
GVIEGPRGTNIHSAKISPEGYISYYNAIVPTTWNIPTMGLATEGFHHEYAPHVIRAYDPCLSCATHMIVKDDETKEVLKD
DMVQL

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen [H]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=566, Percent_Identity=23.6749116607774, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1787207, Length=110, Percent_Identity=34.5454545454545, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017682
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.98.1 [H]

Molecular weight: Translated: 44982; Mature: 44851

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETIVISPTSRQEGHAELSMEVDENGIVTTGRYFSITPVRGIEKMVIGKRPETAPVLVQ
CCCCEEECCCCCCCCCCEEEEEECCCCEEECCCEEEECCHHHHHHHHHCCCCCHHHHHHH
RICGVCPVTHTLASVEAIDDSLKIEVPQAGRILREMCLNAHMINSHAIHHFLVAPDFVPD
HHHCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCHHHCCHHHHHEEECCCCCCC
NQYKEVVKNISTIRKNAQYVEDTVGGEGIHPSDIRIGGMAHNITKNTKNKIKTRISGMMK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
LLEEHVETMAGLIEDKDFPDKLGAHNQPVFASDNTYGSKNNLSINEIEEILPSSWYDLPD
HHHHHHHHHHHHHCCCCCCHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCH
IGERACSQIPLYKGNVVETGPRARASKFRKFKEVGVKAQHLARAQEMINSAEKILELVDK
HHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LDTSAQVMADYTLEGTNRLGVGVIEGPRGTNIHSAKISPEGYISYYNAIVPTTWNIPTMG
HCCCHHHHEEEEECCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCC
LATEGFHHEYAPHVIRAYDPCLSCATHMIVKDDETKEVLKDDMVQL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SETIVISPTSRQEGHAELSMEVDENGIVTTGRYFSITPVRGIEKMVIGKRPETAPVLVQ
CCCEEECCCCCCCCCCEEEEEECCCCEEECCCEEEECCHHHHHHHHHCCCCCHHHHHHH
RICGVCPVTHTLASVEAIDDSLKIEVPQAGRILREMCLNAHMINSHAIHHFLVAPDFVPD
HHHCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCHHHCCHHHHHEEECCCCCCC
NQYKEVVKNISTIRKNAQYVEDTVGGEGIHPSDIRIGGMAHNITKNTKNKIKTRISGMMK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
LLEEHVETMAGLIEDKDFPDKLGAHNQPVFASDNTYGSKNNLSINEIEEILPSSWYDLPD
HHHHHHHHHHHHHCCCCCCHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCH
IGERACSQIPLYKGNVVETGPRARASKFRKFKEVGVKAQHLARAQEMINSAEKILELVDK
HHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LDTSAQVMADYTLEGTNRLGVGVIEGPRGTNIHSAKISPEGYISYYNAIVPTTWNIPTMG
HCCCHHHHEEEEECCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCC
LATEGFHHEYAPHVIRAYDPCLSCATHMIVKDDETKEVLKDDMVQL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2207102; 9371463; 3663585 [H]