Click here to switch to the map view.

The map label for this gene is glmU [H]

Identifier: 84490089

GI number: 84490089

Start: 1465432

End: 1466727

Strand: Direct

Name: glmU [H]

Synonym: Msp_1301

Alternate gene names: 84490089

Gene position: 1465432-1466727 (Clockwise)

Preceding gene: 84490088

Following gene: 84490090

Centisome position: 82.91

GC content: 31.02

Gene sequence:

>1296_bases
ATGAAAGCTATAATTTTAACTGCAGGTGAAGGAACAAGAATGCGTCCTTTAACAACAACACGACCTAAAACAATGCTTAT
AACTGGTGGAAAACCATTAATACAATATAATATTGAATCATTAAGAGATGCTGGAATAAAAGACATTACTCTCGTTGTAG
GCTATAAAAAAGAAGTTATTGAAGATTATTTTGGTGATGGAAGTGAATATGGAGTAAATATTACTTATGCTGTGCAAGAG
GGCCAGTTAGGAACTGCCCATGCCATAGGTAGTGCTGAAGAATATATTGATGAAAGTTTCATAGTTTTAAATGGGGATAT
AATTGTAAGTTACGATCTTATCAGAAATTTAATTGAAAAATATGCAACTAGAACTAGTAACAATGTAAAATCTGTATTAA
CATTAATTGAAGTAGATGATCCATCAAGTTATGGTATTGTTTCAACAGAAAATGGTAAAATAACAGAGATTATTGAAAAA
CCTTCAGTTGAAGATGCTCCAAGTAATCTTGCTAATGCAGGAATTTATTTATTTAGTCCAGAAATATTTGATGCTATAAG
AAAAACTGAGTTATCCAAACGTGGAGAATATGAAATTACTGATTCATTAGATATAGAATTATCTGAAGGATGGGAAATAT
TAGGTCTTATTTCTAATGAAAAATGGATGGATGTTGGAAGACCATGGGAATTATTAGAATGTAATCAGGATTTCCTTGAA
AAAATGGACGATAGTATTGAGGGCGAAATTGAAGATAATGTAACTATTCATGGTCCAGTACATCTTGGTAAAGGTAGTAT
CATTAGATCGGGTTGTTATATTCAAGGTCCTGTATTTATTGGTGAAAACTGTGATGTTGGACCTAACACTTATCTTAGAC
CATATGCATGTTTATGTAACGATATAGATGTTGGTAATGCTGTTGAAATTAAAAATAGTATTATTATGGATGGAACTAAT
GTTAATCATTTATCATATGTTGGAGATTCAGTTATTGGTGTAAATTGTAATCTTGGTGCAGGTACTAATCTTGCTAACTT
ACGTTTTGATGATAAACATGTGCAAGTTACTGTAAAGGGCAATAGAATTGATTCTGGTCGTAGAAAACTTGGAGCTATCT
TTGGTGACGATGTGAAAACAGGAATTAATACTAGTGTTAATCCTGGTGTGAAAATTGGTAATGGTTCCTTTATAAATGCA
GGTTGTGTTTTATATAGAGATATTGAATCATTTTCATTAGTTTCCACAGAAACTAATCTTATTGTTAAGAAGATAAAAGA
AAAAAAAGAGAATTAA

Upstream 100 bases:

>100_bases
ACTTATTTTATATCATTGATTAAAAATAGTATTCATTAAACTATATTAAAAATGTATTACTAATATTTTAAATATACTAA
TATAAAACAGAGGAATACTA

Downstream 100 bases:

>100_bases
ATCCTTTTTTTCTTTTTAATAATAGAAATATTATTTTTTTAAATTAATGATACATATGATACAACCCATTTTTATAAATT
TTTAATACCACAACTATTTT

Product: nucleoside-diphosphate-sugar pyrophosphorylase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKAIILTAGEGTRMRPLTTTRPKTMLITGGKPLIQYNIESLRDAGIKDITLVVGYKKEVIEDYFGDGSEYGVNITYAVQE
GQLGTAHAIGSAEEYIDESFIVLNGDIIVSYDLIRNLIEKYATRTSNNVKSVLTLIEVDDPSSYGIVSTENGKITEIIEK
PSVEDAPSNLANAGIYLFSPEIFDAIRKTELSKRGEYEITDSLDIELSEGWEILGLISNEKWMDVGRPWELLECNQDFLE
KMDDSIEGEIEDNVTIHGPVHLGKGSIIRSGCYIQGPVFIGENCDVGPNTYLRPYACLCNDIDVGNAVEIKNSIIMDGTN
VNHLSYVGDSVIGVNCNLGAGTNLANLRFDDKHVQVTVKGNRIDSGRRKLGAIFGDDVKTGINTSVNPGVKIGNGSFINA
GCVLYRDIESFSLVSTETNLIVKKIKEKKEN

Sequences:

>Translated_431_residues
MKAIILTAGEGTRMRPLTTTRPKTMLITGGKPLIQYNIESLRDAGIKDITLVVGYKKEVIEDYFGDGSEYGVNITYAVQE
GQLGTAHAIGSAEEYIDESFIVLNGDIIVSYDLIRNLIEKYATRTSNNVKSVLTLIEVDDPSSYGIVSTENGKITEIIEK
PSVEDAPSNLANAGIYLFSPEIFDAIRKTELSKRGEYEITDSLDIELSEGWEILGLISNEKWMDVGRPWELLECNQDFLE
KMDDSIEGEIEDNVTIHGPVHLGKGSIIRSGCYIQGPVFIGENCDVGPNTYLRPYACLCNDIDVGNAVEIKNSIIMDGTN
VNHLSYVGDSVIGVNCNLGAGTNLANLRFDDKHVQVTVKGNRIDSGRRKLGAIFGDDVKTGINTSVNPGVKIGNGSFINA
GCVLYRDIESFSLVSTETNLIVKKIKEKKEN
>Mature_431_residues
MKAIILTAGEGTRMRPLTTTRPKTMLITGGKPLIQYNIESLRDAGIKDITLVVGYKKEVIEDYFGDGSEYGVNITYAVQE
GQLGTAHAIGSAEEYIDESFIVLNGDIIVSYDLIRNLIEKYATRTSNNVKSVLTLIEVDDPSSYGIVSTENGKITEIIEK
PSVEDAPSNLANAGIYLFSPEIFDAIRKTELSKRGEYEITDSLDIELSEGWEILGLISNEKWMDVGRPWELLECNQDFLE
KMDDSIEGEIEDNVTIHGPVHLGKGSIIRSGCYIQGPVFIGENCDVGPNTYLRPYACLCNDIDVGNAVEIKNSIIMDGTN
VNHLSYVGDSVIGVNCNLGAGTNLANLRFDDKHVQVTVKGNRIDSGRRKLGAIFGDDVKTGINTSVNPGVKIGNGSFINA
GCVLYRDIESFSLVSTETNLIVKKIKEKKEN

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetyl-glucosamine (UDP- GlcNAc). Responsible for the acetylation of GlcN-1-P to GlcNAc-1- P, and for the uridyl transfer from UTP to GlcNAc-1-P, to produce UDP-GlcN

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=353, Percent_Identity=28.328611898017, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI11761619, Length=350, Percent_Identity=28.8571428571429, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI31881779, Length=376, Percent_Identity=23.936170212766, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI45447090, Length=376, Percent_Identity=23.936170212766, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI1790168, Length=468, Percent_Identity=28.6324786324786, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788351, Length=240, Percent_Identity=32.9166666666667, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790224, Length=242, Percent_Identity=30.9917355371901, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787488, Length=238, Percent_Identity=30.2521008403361, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788355, Length=266, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI133931050, Length=352, Percent_Identity=28.125, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17509979, Length=351, Percent_Identity=25.3561253561254, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17509981, Length=338, Percent_Identity=24.8520710059172, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=352, Percent_Identity=30.3977272727273, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI21355443, Length=343, Percent_Identity=28.8629737609329, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24644084, Length=343, Percent_Identity=28.8629737609329, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24653912, Length=374, Percent_Identity=24.8663101604278, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.23; =2.3.1.157 [H]

Molecular weight: Translated: 47222; Mature: 47222

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIILTAGEGTRMRPLTTTRPKTMLITGGKPLIQYNIESLRDAGIKDITLVVGYKKEVI
CEEEEEECCCCCEECCEECCCCCEEEEECCCCEEEEEHHHHHCCCCCEEEEEECCHHHHH
EDYFGDGSEYGVNITYAVQEGQLGTAHAIGSAEEYIDESFIVLNGDIIVSYDLIRNLIEK
HHHCCCCCCCCEEEEEEEECCCCCCHHHCCCHHHHCCCEEEEEECCEEEEHHHHHHHHHH
YATRTSNNVKSVLTLIEVDDPSSYGIVSTENGKITEIIEKPSVEDAPSNLANAGIYLFSP
HHHCCCCCCEEEEEEEEECCCCCCCEEECCCCCEEEEECCCCCCCCCCCHHHCCEEEECH
EIFDAIRKTELSKRGEYEITDSLDIELSEGWEILGLISNEKWMDVGRPWELLECNQDFLE
HHHHHHHHHHHHCCCCEEECCCEEEEECCCCEEEEEECCCCEEECCCCHHHHHCCHHHHH
KMDDSIEGEIEDNVTIHGPVHLGKGSIIRSGCYIQGPVFIGENCDVGPNTYLRPYACLCN
HHCCCCCCEECCCEEEEECEEECCCCEEECCCEEECCEEECCCCCCCCCCCCCCHHHHCC
DIDVGNAVEIKNSIIMDGTNVNHLSYVGDSVIGVNCNLGAGTNLANLRFDDKHVQVTVKG
CCCCCCEEEECCEEEECCCCCCEEEECCCCEEEEEECCCCCCCEEEEEECCCEEEEEEEC
NRIDSGRRKLGAIFGDDVKTGINTSVNPGVKIGNGSFINAGCVLYRDIESFSLVSTETNL
CCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEECCEEEEEECCCEEEEECCHHH
IVKKIKEKKEN
HHHHHHHHCCC
>Mature Secondary Structure
MKAIILTAGEGTRMRPLTTTRPKTMLITGGKPLIQYNIESLRDAGIKDITLVVGYKKEVI
CEEEEEECCCCCEECCEECCCCCEEEEECCCCEEEEEHHHHHCCCCCEEEEEECCHHHHH
EDYFGDGSEYGVNITYAVQEGQLGTAHAIGSAEEYIDESFIVLNGDIIVSYDLIRNLIEK
HHHCCCCCCCCEEEEEEEECCCCCCHHHCCCHHHHCCCEEEEEECCEEEEHHHHHHHHHH
YATRTSNNVKSVLTLIEVDDPSSYGIVSTENGKITEIIEKPSVEDAPSNLANAGIYLFSP
HHHCCCCCCEEEEEEEEECCCCCCCEEECCCCCEEEEECCCCCCCCCCCHHHCCEEEECH
EIFDAIRKTELSKRGEYEITDSLDIELSEGWEILGLISNEKWMDVGRPWELLECNQDFLE
HHHHHHHHHHHHCCCCEEECCCEEEEECCCCEEEEEECCCCEEECCCCHHHHHCCHHHHH
KMDDSIEGEIEDNVTIHGPVHLGKGSIIRSGCYIQGPVFIGENCDVGPNTYLRPYACLCN
HHCCCCCCEECCCEEEEECEEECCCCEEECCCEEECCEEECCCCCCCCCCCCCCHHHHCC
DIDVGNAVEIKNSIIMDGTNVNHLSYVGDSVIGVNCNLGAGTNLANLRFDDKHVQVTVKG
CCCCCCEEEECCEEEECCCCCCEEEECCCCEEEEEECCCCCCCEEEEEECCCEEEEEEEC
NRIDSGRRKLGAIFGDDVKTGINTSVNPGVKIGNGSFINAGCVLYRDIESFSLVSTETNL
CCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEECCEEEEEECCCEEEEECCHHH
IVKKIKEKKEN
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]