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The map label for this gene is pyrE

Identifier: 84489831

GI number: 84489831

Start: 1181516

End: 1182052

Strand: Direct

Name: pyrE

Synonym: Msp_1036

Alternate gene names: 84489831

Gene position: 1181516-1182052 (Clockwise)

Preceding gene: 84489830

Following gene: 84489832

Centisome position: 66.85

GC content: 26.44

Gene sequence:

>537_bases
ATGACAGACTATAAAAATAAATTAATAAACTTATTAAAAGAAAATAATGTAATTAAATTTGGAAAATTCACATTATCTTC
AGGACGTGAAAGTGACTACTATGTTGATATGAAAAAAGCAATAACTGAACCTGAAATACTAGATTGTGTAGCTCATCTTA
TAACCAATGAAATAGAACATGATAATATTGATAAAATAGCAGGACCTGCATTAGGAGCAGTTCCTATTGCTACAGCAACA
TCATTAATATCAAAAAAACCAATGTTAATGATTAGAAAAGCTAAAAAAACATATGGTACAAGTAAACAAATTGAAGGTGA
ATTACTTGAAAATGATGATGTTGTAATAGTTGAAGATGTTACAACAACTGGTGGTTCTTTATTAAAAGCCATAAATGTTA
TTGAAGATAATGGTGGAAATATAGTTAAAGCATTTGTAATTGTAGATAGAGAAGAAGGTGCTCAAGAAACATTTAAAGAA
AATAACATTGAATTCACACCACTTTTAACTATTAGTGAATTTAAAAAATATTTATAA

Upstream 100 bases:

>100_bases
GATAAACCGTATTGGTGATAAAGTAATTCTTAAAAAAGCAATAAATGAAGCAGAACAACTTGTTGAAGAATTAGAAAATG
AAATATAAGGACATATTACA

Downstream 100 bases:

>100_bases
TTCTAACTAAAAAATAACTCTATACTAAATAAAAAACAAGTTATTTCTGTAGATACCAACACCACATTTAATAAATAAAT
ATTTAAACTATTATATTATA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MTDYKNKLINLLKENNVIKFGKFTLSSGRESDYYVDMKKAITEPEILDCVAHLITNEIEHDNIDKIAGPALGAVPIATAT
SLISKKPMLMIRKAKKTYGTSKQIEGELLENDDVVIVEDVTTTGGSLLKAINVIEDNGGNIVKAFVIVDREEGAQETFKE
NNIEFTPLLTISEFKKYL

Sequences:

>Translated_178_residues
MTDYKNKLINLLKENNVIKFGKFTLSSGRESDYYVDMKKAITEPEILDCVAHLITNEIEHDNIDKIAGPALGAVPIATAT
SLISKKPMLMIRKAKKTYGTSKQIEGELLENDDVVIVEDVTTTGGSLLKAINVIEDNGGNIVKAFVIVDREEGAQETFKE
NNIEFTPLLTISEFKKYL
>Mature_177_residues
TDYKNKLINLLKENNVIKFGKFTLSSGRESDYYVDMKKAITEPEILDCVAHLITNEIEHDNIDKIAGPALGAVPIATATS
LISKKPMLMIRKAKKTYGTSKQIEGELLENDDVVIVEDVTTTGGSLLKAINVIEDNGGNIVKAFVIVDREEGAQETFKEN
NIEFTPLLTISEFKKYL

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=173, Percent_Identity=31.2138728323699, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1790073, Length=193, Percent_Identity=26.9430051813472, Blast_Score=74, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17554964, Length=177, Percent_Identity=32.2033898305085, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17508631, Length=162, Percent_Identity=32.7160493827161, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323530, Length=162, Percent_Identity=28.3950617283951, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323927, Length=167, Percent_Identity=27.5449101796407, Blast_Score=63, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17933654, Length=163, Percent_Identity=33.1288343558282, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_METST (Q2NFI3)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_448063.1
- ProteinModelPortal:   Q2NFI3
- SMR:   Q2NFI3
- STRING:   Q2NFI3
- GeneID:   3854988
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_1036
- NMPDR:   fig|339860.6.peg.990
- eggNOG:   arNOG09150
- HOGENOM:   HBG404341
- OMA:   LSYPRIR
- PhylomeDB:   Q2NFI3
- ProtClustDB:   PRK00455
- BioCyc:   MSTA339860:MSP_1036-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 19767; Mature: 19636

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 92-92 BINDING 93-93 BINDING 96-96 BINDING 122-122 BINDING 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYKNKLINLLKENNVIKFGKFTLSSGRESDYYVDMKKAITEPEILDCVAHLITNEIEH
CCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEHHHHCCCCHHHHHHHHHHHCCCCC
DNIDKIAGPALGAVPIATATSLISKKPMLMIRKAKKTYGTSKQIEGELLENDDVVIVEDV
CCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEC
TTTGGSLLKAINVIEDNGGNIVKAFVIVDREEGAQETFKENNIEFTPLLTISEFKKYL
CCCCHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHCCCEEEEEEEHHHHHHCC
>Mature Secondary Structure 
TDYKNKLINLLKENNVIKFGKFTLSSGRESDYYVDMKKAITEPEILDCVAHLITNEIEH
CCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEHHHHCCCCHHHHHHHHHHHCCCCC
DNIDKIAGPALGAVPIATATSLISKKPMLMIRKAKKTYGTSKQIEGELLENDDVVIVEDV
CCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEC
TTTGGSLLKAINVIEDNGGNIVKAFVIVDREEGAQETFKENNIEFTPLLTISEFKKYL
CCCCHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHCCCEEEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA