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The map label for this gene is gshA-2

Identifier: 83721647

GI number: 83721647

Start: 455543

End: 456832

Strand: Direct

Name: gshA-2

Synonym: BTH_I0409

Alternate gene names: NA

Gene position: 455543-456832 (Clockwise)

Preceding gene: 83719507

Following gene: 83720937

Centisome position: 11.96

GC content: 66.43

Gene sequence:

>1290_bases
ATGGTTCCCCATCTCGTTACGGCGTTAAACGGCCCGCTGCTCGAGCTCGAGCGGAAGATCCTCGACGCCACGCCTGCGAT
CGAACGCTGGTTCAGGCTCGAATGGCAGGAGCACACGCCGCCGTTCTACTGTTCGGTCGATCTGCGCAACGCGGGCTTCA
AGCTCGCGCCCGTCGACGCGAACCTGTTCCCGGGCGCGTTCAACAATCTGCCGCCCGAAGTGCTGCCGCTCGCCGTGCAG
GCGGCGATGGCCGCGATCGAGAAGATCTGCCCGGACGCGAAGAATCTGCTCGTGATTCCCGAGCTGCCGACGCGCAACGC
GTTCTATCTCGAGAACGTCGCGCGCCTCGCGACGATCATGCGCCAGGCGGGCCTGAACGTGCGCTTCGGCTCGCTCGATC
CGGGCATCGTCGAGGTCACGCCGATCACGCTCGCCGACGGCCAGAAGATCGTGCTCGAGCCGCTCGAGCGCACGCCGCGC
CGCCTCGGCCTGAAGAATTTCGACCCTTGCTCGATCCTGCTGAACAACGATCTGTCGGCGGGCATCCCGAGCGTGCTGGA
AAACCTGCACGAGCAGTATCTGCTGCCGCCGCTGCACGCCGGCTGGGCGGTGCGCCGAAAGTCGACGCACTTCTCCTGCT
ATGACGACGTCGCGAAGAAGTTCGCGAAGATGGTGGGCGTCGATCCGTGGATGCTGAATCCGTATTTCTCGCACGTCGAA
GGCGTCGACTGGCACGCGAACGACGGCGGCGAGGCGCTTGCCGAAGCGATCGACGCGGTGCTCAAGAAGATCGCGCGCAA
GTACCGCGAATACGGGATCGGCGAGAAGCCGTACGTCGTCGTGAAGGCGGACGCCGGCACGGCGGGGCGCGGCGTGATGA
CGGTGCACGACGCGTCCGAGATCGGCCGGATGACGAAGCACGAGCGCGCGCAGATGGCCGAATCGAAGGCGGGCGTGCCG
GTGCGCGACGTGATCGTGCAGGAAGGCGTCTACACGTTCGAGCGCATCGGCGACGAGGTGGCCGAGCCTGTCGTCTACAT
GATCGACCGCTACGTGGTGGGCGGGTTCTACCGCACACACGGCGCGCGCGAGCGCGACCAGAATCTGAATGCGCCCGGCA
TGCACTACGTGCCGCTCGGCTTCGAGCACACGGCGCTGCCGGACACGCACGCGAAGCCCGGCGCGGCGCCGCCGAACCGC
TTCTACATGTATGGCGTCGTCGCACGGCTTGGGCTGCTCGCGTCGTCGGTCGAGCTCGAGAAGACCGACCCCGAAGCGAT
CCAGGTGTGA

Upstream 100 bases:

>100_bases
AGAGACAATCATGCGGTAAATCCTTGCGATACCGGGAACAATCCCCAAATGCGACGGGCAATTGCTCTACAATCTTTTTT
CTCCGGTCCGCGAGTCACAC

Downstream 100 bases:

>100_bases
CGCGCTCCCTCTAGCATCAGGATTCGCATGGACATTCTCTTTATCGCCGACCCGCTCGAGCGCTTCAAGATCTACAAGGA
CTCGACCTACGCGATGATGG

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MVPHLVTALNGPLLELERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDANLFPGAFNNLPPEVLPLAVQ
AAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIMRQAGLNVRFGSLDPGIVEVTPITLADGQKIVLEPLERTPR
RLGLKNFDPCSILLNNDLSAGIPSVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMLNPYFSHVE
GVDWHANDGGEALAEAIDAVLKKIARKYREYGIGEKPYVVVKADAGTAGRGVMTVHDASEIGRMTKHERAQMAESKAGVP
VRDVIVQEGVYTFERIGDEVAEPVVYMIDRYVVGGFYRTHGARERDQNLNAPGMHYVPLGFEHTALPDTHAKPGAAPPNR
FYMYGVVARLGLLASSVELEKTDPEAIQV

Sequences:

>Translated_429_residues
MVPHLVTALNGPLLELERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDANLFPGAFNNLPPEVLPLAVQ
AAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIMRQAGLNVRFGSLDPGIVEVTPITLADGQKIVLEPLERTPR
RLGLKNFDPCSILLNNDLSAGIPSVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMLNPYFSHVE
GVDWHANDGGEALAEAIDAVLKKIARKYREYGIGEKPYVVVKADAGTAGRGVMTVHDASEIGRMTKHERAQMAESKAGVP
VRDVIVQEGVYTFERIGDEVAEPVVYMIDRYVVGGFYRTHGARERDQNLNAPGMHYVPLGFEHTALPDTHAKPGAAPPNR
FYMYGVVARLGLLASSVELEKTDPEAIQV
>Mature_429_residues
MVPHLVTALNGPLLELERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDANLFPGAFNNLPPEVLPLAVQ
AAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIMRQAGLNVRFGSLDPGIVEVTPITLADGQKIVLEPLERTPR
RLGLKNFDPCSILLNNDLSAGIPSVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMLNPYFSHVE
GVDWHANDGGEALAEAIDAVLKKIARKYREYGIGEKPYVVVKADAGTAGRGVMTVHDASEIGRMTKHERAQMAESKAGVP
VRDVIVQEGVYTFERIGDEVAEPVVYMIDRYVVGGFYRTHGARERDQNLNAPGMHYVPLGFEHTALPDTHAKPGAAPPNR
FYMYGVVARLGLLASSVELEKTDPEAIQV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 47469; Mature: 47469

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHLVTALNGPLLELERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDA
CCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGAFNNLPPEVLPLAVQAAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIM
CCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
RQAGLNVRFGSLDPGIVEVTPITLADGQKIVLEPLERTPRRLGLKNFDPCSILLNNDLSA
HHCCCCEEECCCCCCEEEEEEEEECCCCEEECCHHHHCHHHCCCCCCCCEEEEECCCCCC
GIPSVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMLNPYFSHVE
CHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEECHHHHHCC
GVDWHANDGGEALAEAIDAVLKKIARKYREYGIGEKPYVVVKADAGTAGRGVMTVHDASE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECHHH
IGRMTKHERAQMAESKAGVPVRDVIVQEGVYTFERIGDEVAEPVVYMIDRYVVGGFYRTH
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
GARERDQNLNAPGMHYVPLGFEHTALPDTHAKPGAAPPNRFYMYGVVARLGLLASSVELE
CCCHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
KTDPEAIQV
CCCCHHCCC
>Mature Secondary Structure
MVPHLVTALNGPLLELERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDA
CCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGAFNNLPPEVLPLAVQAAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIM
CCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
RQAGLNVRFGSLDPGIVEVTPITLADGQKIVLEPLERTPRRLGLKNFDPCSILLNNDLSA
HHCCCCEEECCCCCCEEEEEEEEECCCCEEECCHHHHCHHHCCCCCCCCEEEEECCCCCC
GIPSVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMLNPYFSHVE
CHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEECHHHHHCC
GVDWHANDGGEALAEAIDAVLKKIARKYREYGIGEKPYVVVKADAGTAGRGVMTVHDASE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECHHH
IGRMTKHERAQMAESKAGVPVRDVIVQEGVYTFERIGDEVAEPVVYMIDRYVVGGFYRTH
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
GARERDQNLNAPGMHYVPLGFEHTALPDTHAKPGAAPPNRFYMYGVVARLGLLASSVELE
CCCHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
KTDPEAIQV
CCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA