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The map label for this gene is amt [H]

Identifier: 83719507

GI number: 83719507

Start: 453804

End: 455213

Strand: Direct

Name: amt [H]

Synonym: BTH_I0408

Alternate gene names: 83719507

Gene position: 453804-455213 (Clockwise)

Preceding gene: 83718613

Following gene: 83721647

Centisome position: 11.91

GC content: 68.79

Gene sequence:

>1410_bases
ATGGCCGACGACGCGTCGTCGGCACCCGCCGCCGCATCGGCCGCAACGGCTTCCGACACGTCGGCGAGCGCCGCCGTCGC
GTCCGCGCCGGCCGCCGCTTCCGCGGCCGCTTCGGCCGCCGCGCCGGCTTCGGCCGCCGCCGCGCCCGCCGCGCCGACCG
CGCCGACCGCGCCGTTCTCCGTCGATTCGTCGAAGATCAATTCGGGCGACACCGCGTGGATGCTCACGTCGACCGCGCTC
GTGCTGTTCATGACGATCCCGGGCCTCGCGCTCTTCTACGGTGGCATGGTCCGCAAGAAGAACGTGCTCGCGACCGTGAT
GCAGAGCTTCGCGATCACCGCGCTGATCACGGTCCTCTGGACGGTGGTCGGCTACAGCCTCGCGTTCACGCCGGGCAACG
GCTTCATCGGCGGCTTCTCGCGCGTGTTCCTGTCCGGGATGAACTACATCCACGGCGACAAGGCGACGACGCTCACCGTC
AGCCACCTCGCGCAGACGATCCCGGAATCGGTCTACTTCGTCTATCAGATGACGTTCGCGATCATCACGCCGGCCCTCAT
CTGCGGCGCGTTCGCCGACCGGATGAAGTTCTCGGCGATGCTCGTCTTCATGACGCTCTGGTCGCTGATCGTCTACGTGC
CGATCGCGCACATGGTCTGGGAGCCGACGGGCTGGCTGTCGTCGGACGGCGTGCTCGACTTCGCGGGCGGCACGGTCGTG
CACATCAACGCCGGCATCGCGGGCCTCGTGTCGTGCCTCATGCTCGGCAAGCGCGTCGGCTACGGCCGCGAAGCGATGGC
GCCGCACAACCTCGTGCTGACGCTGATCGGCGGCTCGATGCTGTGGGTCGGCTGGTTCGGCTTCAACGCGGGCTCCGCGG
TGGCCGCCGACGGCCGCGCCGGCTTCGCGATGCTGACGACGCAAGTCGCCACCGCGTGCGCGGCGCTCGGCTGGATGTTC
GCCGAGTGGATCGCGAAGGGTAAGCCTTCGGTGCTCGGCATCGTGTCGGGCGCGGTCGCGGGTCTCGTCGCGATCACGCC
GGCGGCCGGCTTCGTCGGCGTCGCGGGCGCGCTCACGATCGGCGTCGCGGCGGGCGTCGTGTGCTTCTGGTCGGCGACGT
GGCTCAAGCACAAGCTCGGCTACGACGATTCGCTCGACGCGTTCGGCGTGCACGGCGTGGGCGGCATTCTCGGCGCGCTG
CTGACGGGCGTGTTCGCGGTCAAGGACATCGGCGGCGTCGACGGCAGCCTGCTGCTGCAGGCAAAGGGCGTCGCGATCAC
GCTTGTCTACAGCGGCATCGTCAGCTTCGTGCTGCTGAAGGTGATCGACCTCGTGATCGGCCTGCGTGTGACGGAAGAAG
AAGAACGAGAAGGCCTCGACGTGATCCTGCATGGCGAGCACGTCGAATAA

Upstream 100 bases:

>100_bases
AACAAGCGACACAAGCGATAAGAGGAAAACCAAGATGCGCAAGATCCTGATGTCCCTGTTGATGGCCGGCTCGCTGCTTG
CGGGCGGCATCGGCGCCGCG

Downstream 100 bases:

>100_bases
TGGGATCCATCCCCGAACCGGGGGCGGCGAAGCGCTCGCACGCTTCGCCGCCCGCAGAATGAGCGCAGCGATTTCGGCCC
GCCCTCGAGGCGGGTTTTTT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MADDASSAPAAASAATASDTSASAAVASAPAAASAAASAAAPASAAAAPAAPTAPTAPFSVDSSKINSGDTAWMLTSTAL
VLFMTIPGLALFYGGMVRKKNVLATVMQSFAITALITVLWTVVGYSLAFTPGNGFIGGFSRVFLSGMNYIHGDKATTLTV
SHLAQTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMTLWSLIVYVPIAHMVWEPTGWLSSDGVLDFAGGTVV
HINAGIAGLVSCLMLGKRVGYGREAMAPHNLVLTLIGGSMLWVGWFGFNAGSAVAADGRAGFAMLTTQVATACAALGWMF
AEWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVAGALTIGVAAGVVCFWSATWLKHKLGYDDSLDAFGVHGVGGILGAL
LTGVFAVKDIGGVDGSLLLQAKGVAITLVYSGIVSFVLLKVIDLVIGLRVTEEEEREGLDVILHGEHVE

Sequences:

>Translated_469_residues
MADDASSAPAAASAATASDTSASAAVASAPAAASAAASAAAPASAAAAPAAPTAPTAPFSVDSSKINSGDTAWMLTSTAL
VLFMTIPGLALFYGGMVRKKNVLATVMQSFAITALITVLWTVVGYSLAFTPGNGFIGGFSRVFLSGMNYIHGDKATTLTV
SHLAQTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMTLWSLIVYVPIAHMVWEPTGWLSSDGVLDFAGGTVV
HINAGIAGLVSCLMLGKRVGYGREAMAPHNLVLTLIGGSMLWVGWFGFNAGSAVAADGRAGFAMLTTQVATACAALGWMF
AEWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVAGALTIGVAAGVVCFWSATWLKHKLGYDDSLDAFGVHGVGGILGAL
LTGVFAVKDIGGVDGSLLLQAKGVAITLVYSGIVSFVLLKVIDLVIGLRVTEEEEREGLDVILHGEHVE
>Mature_468_residues
ADDASSAPAAASAATASDTSASAAVASAPAAASAAASAAAPASAAAAPAAPTAPTAPFSVDSSKINSGDTAWMLTSTALV
LFMTIPGLALFYGGMVRKKNVLATVMQSFAITALITVLWTVVGYSLAFTPGNGFIGGFSRVFLSGMNYIHGDKATTLTVS
HLAQTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMTLWSLIVYVPIAHMVWEPTGWLSSDGVLDFAGGTVVH
INAGIAGLVSCLMLGKRVGYGREAMAPHNLVLTLIGGSMLWVGWFGFNAGSAVAADGRAGFAMLTTQVATACAALGWMFA
EWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVAGALTIGVAAGVVCFWSATWLKHKLGYDDSLDAFGVHGVGGILGALL
TGVFAVKDIGGVDGSLLLQAKGVAITLVYSGIVSFVLLKVIDLVIGLRVTEEEEREGLDVILHGEHVE

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=409, Percent_Identity=51.5892420537897, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17550182, Length=451, Percent_Identity=28.3813747228381, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17550180, Length=463, Percent_Identity=25.0539956803456, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32564042, Length=433, Percent_Identity=27.2517321016166, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=458, Percent_Identity=24.8908296943231, Blast_Score=99, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=35.8139534883721, Blast_Score=236, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6325396, Length=425, Percent_Identity=34.1176470588235, Blast_Score=222, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6321559, Length=414, Percent_Identity=32.3671497584541, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI161078314, Length=418, Percent_Identity=28.2296650717703, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47879; Mature: 47748

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDASSAPAAASAATASDTSASAAVASAPAAASAAASAAAPASAAAAPAAPTAPTAPFS
CCCCCCCCCCCHHCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
VDSSKINSGDTAWMLTSTALVLFMTIPGLALFYGGMVRKKNVLATVMQSFAITALITVLW
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
TVVGYSLAFTPGNGFIGGFSRVFLSGMNYIHGDKATTLTVSHLAQTIPESVYFVYQMTFA
HHHCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
IITPALICGAFADRMKFSAMLVFMTLWSLIVYVPIAHMVWEPTGWLSSDGVLDFAGGTVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEE
HINAGIAGLVSCLMLGKRVGYGREAMAPHNLVLTLIGGSMLWVGWFGFNAGSAVAADGRA
EECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCEEECCCCC
GFAMLTTQVATACAALGWMFAEWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVAGALTI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GVAAGVVCFWSATWLKHKLGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGVDGSLLLQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
AKGVAITLVYSGIVSFVLLKVIDLVIGLRVTEEEEREGLDVILHGEHVE
ECCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCCC
>Mature Secondary Structure 
ADDASSAPAAASAATASDTSASAAVASAPAAASAAASAAAPASAAAAPAAPTAPTAPFS
CCCCCCCCCCHHCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
VDSSKINSGDTAWMLTSTALVLFMTIPGLALFYGGMVRKKNVLATVMQSFAITALITVLW
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
TVVGYSLAFTPGNGFIGGFSRVFLSGMNYIHGDKATTLTVSHLAQTIPESVYFVYQMTFA
HHHCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
IITPALICGAFADRMKFSAMLVFMTLWSLIVYVPIAHMVWEPTGWLSSDGVLDFAGGTVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEE
HINAGIAGLVSCLMLGKRVGYGREAMAPHNLVLTLIGGSMLWVGWFGFNAGSAVAADGRA
EECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCEEECCCCC
GFAMLTTQVATACAALGWMFAEWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVAGALTI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GVAAGVVCFWSATWLKHKLGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGVDGSLLLQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
AKGVAITLVYSGIVSFVLLKVIDLVIGLRVTEEEEREGLDVILHGEHVE
ECCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]