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The map label for this gene is leuE [H]

Identifier: 83721610

GI number: 83721610

Start: 1610159

End: 1610773

Strand: Reverse

Name: leuE [H]

Synonym: BTH_I1426

Alternate gene names: 83721610

Gene position: 1610773-1610159 (Counterclockwise)

Preceding gene: 83719012

Following gene: 83720484

Centisome position: 42.29

GC content: 68.62

Gene sequence:

>615_bases
ATGACGGTCAACCCCACTCTCGCCTTCATCGCGACGTGCATCGGCTTCGGCTTCATTCCGGGCCCGGCGCTGCTGCAAAC
CGTATCGCTAACGTTGCAGCACGGCCGCCGCGCGGGTGTGCTGTCGGCGCTCGGCATCCACCTCGGCGCGTTCTTCCAGA
TCTGCGCGGTCGCGATCGGCGCGGTCGTCGTGCTCGACACGTCGCCGTGGCTCTATCATGCGCTGCGCGTCGCGGGCGGC
GGCTATCTGATCTGGCTCGGCATCCAGCGCATCCGCGCGCGCGCCGACGACAGCGGTGCGCCGTCCGCCGTGCCGAAAAA
CGTGATCTCGTCGAGCGCGCTCATCGAGGCGTCGAATCCGAAATCGGCGCTGTTCTATCTGTCGTTCCTGTTGCAGTTCG
TCGACCCGTCCGCTTCGCTCGACGTCGGCTGGCAACTCTTCCTGCTCGGCGCGGGCGCGAACCTGCTGTTCTCGCTGGCC
GATCTGACCTGCATCGCGCTCGCGCATCCGCTGCGCAAGCGCGCGGCGCCGGGCGGCGCCGCGATGACGATCGGGCGCTA
TCTCGCAGGCGCGCTCTTCATCGCGCTCGGCGCCGTCGCGATCGTCGAGCGCTGA

Upstream 100 bases:

>100_bases
ACGAGCATCGGCGCGCCGCCGCCGAAACGCGCGTCGGCCGTGTCGGGCCGGCTAGCATCGGCAGCCGCCGGCCCCGCGCT
TCCTCGACGAGCGATTCCCC

Downstream 100 bases:

>100_bases
CGCGTGCGGGCGATCGAGCCGCGTCCCGCGCATCGCCGCCGACGCTCCGACGCTCCGACGCTCCGACGCTTCGGCTGCGG
CCGGAACGGCGTCGCGCGCC

Product: LysE family protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MTVNPTLAFIATCIGFGFIPGPALLQTVSLTLQHGRRAGVLSALGIHLGAFFQICAVAIGAVVVLDTSPWLYHALRVAGG
GYLIWLGIQRIRARADDSGAPSAVPKNVISSSALIEASNPKSALFYLSFLLQFVDPSASLDVGWQLFLLGAGANLLFSLA
DLTCIALAHPLRKRAAPGGAAMTIGRYLAGALFIALGAVAIVER

Sequences:

>Translated_204_residues
MTVNPTLAFIATCIGFGFIPGPALLQTVSLTLQHGRRAGVLSALGIHLGAFFQICAVAIGAVVVLDTSPWLYHALRVAGG
GYLIWLGIQRIRARADDSGAPSAVPKNVISSSALIEASNPKSALFYLSFLLQFVDPSASLDVGWQLFLLGAGANLLFSLA
DLTCIALAHPLRKRAAPGGAAMTIGRYLAGALFIALGAVAIVER
>Mature_203_residues
TVNPTLAFIATCIGFGFIPGPALLQTVSLTLQHGRRAGVLSALGIHLGAFFQICAVAIGAVVVLDTSPWLYHALRVAGGG
YLIWLGIQRIRARADDSGAPSAVPKNVISSSALIEASNPKSALFYLSFLLQFVDPSASLDVGWQLFLLGAGANLLFSLAD
LTCIALAHPLRKRAAPGGAAMTIGRYLAGALFIALGAVAIVER

Specific function: Exporter of leucine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=140, Percent_Identity=30.7142857142857, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788099, Length=142, Percent_Identity=29.5774647887324, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21225; Mature: 21094

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVNPTLAFIATCIGFGFIPGPALLQTVSLTLQHGRRAGVLSALGIHLGAFFQICAVAIG
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVVVLDTSPWLYHALRVAGGGYLIWLGIQRIRARADDSGAPSAVPKNVISSSALIEASNP
HEEEEECCHHHHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCHHHHHHHCCHHEEECCCC
KSALFYLSFLLQFVDPSASLDVGWQLFLLGAGANLLFSLADLTCIALAHPLRKRAAPGGA
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AMTIGRYLAGALFIALGAVAIVER
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVNPTLAFIATCIGFGFIPGPALLQTVSLTLQHGRRAGVLSALGIHLGAFFQICAVAIG
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVVVLDTSPWLYHALRVAGGGYLIWLGIQRIRARADDSGAPSAVPKNVISSSALIEASNP
HEEEEECCHHHHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCHHHHHHHCCHHEEECCCC
KSALFYLSFLLQFVDPSASLDVGWQLFLLGAGANLLFSLADLTCIALAHPLRKRAAPGGA
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AMTIGRYLAGALFIALGAVAIVER
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA