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The map label for this gene is gatA [H]

Identifier: 83721575

GI number: 83721575

Start: 178418

End: 179908

Strand: Direct

Name: gatA [H]

Synonym: BTH_I0148

Alternate gene names: 83721575

Gene position: 178418-179908 (Clockwise)

Preceding gene: 83720669

Following gene: 83718877

Centisome position: 4.68

GC content: 70.36

Gene sequence:

>1491_bases
ATGCACGCAAAAAGCCTGACCGAACTGCGCGCCGCGCTCGACGCCAAGGAATGCTCGGCCGTCGAGCTGGCGCAGCACTA
TCTGAAACGGATCGACGCCGCGCGCGATCTGAACGCGTTCGTCCACGTCGACGCCGAGCTGACGCTCGCGCAGGCGAAGG
CCGCCGACGCCGCGCTCGCCAAGGGCGAGGCGGGCCCGCTCGCCGGCCTGCCGATCGCGCACAAGGACGTGTTCGTCACG
CGCGGCTGGCGCTCCACCGCCGGCTCGAAGATGCTCGCGAACTACGCGAGCCCGTTCGACGCGACCGTCGTCGCCCGCCT
GTCGGCGGCCGGCATGGTCACGCTCGGCAAGACCAACATGGACGAGTTCGCGATGGGCTCGTCGAACGAGAACTCCGCGT
TCGGCCCGGTGAAGAACCCGTGGGACACGAACGCGGTGCCGGGCGGCAGCTCGGGCGGCAGCTCCGCGGCCGTCGCCGCG
CGCCTCGCGCCCGCCGCGACGGGCACCGACACGGGCGGCTCGATCCGCCAGCCGGCGTCGTTCGCCGGCGTGACGGGCAT
CAAGCCGACCTACGGGCGCGTGTCGCGCTACGGGATGATCGCGTTCGCGTCGTCGCTCGACCAGGGCGGCCCGATGGCGC
GGAGCGCGGCCGACTGCGCGCTCCTGCTGAACGCGATGGCGGGCTTCGACGAGCGCGATTCGACGAGCCTCGAGCGCGCC
GACGAAGACTACACGCGCCACCTCGGCAAGACCTGGGCGGCCGGCGGCGACGCGGGCAAGCCGCTCGCCGGCCTGCGCAT
CGGCCTGCCGGCCGAGTATTTCGGCGCGGGCCTCGCCGACGACGTGCGCGCGGCGATCGACGCGGCGCTCAAGACCTATG
AGACGCTCGGCGCGACGCTCGTGCCGGTGTCGCTGCCGAAGACCGAGCTGTCGATCCCCGTCTACTACGTGATCGCGCCC
GCCGAGGCGTCGTCGAACCTGTCGCGCTTCGACGGCGTGCGCTACGGTCACCGCGCGGCCGAATACCGCGATCTGCTCGA
CATGTACAAGAAGTCGCGCGCCGAGGGCTTCGGGCCCGAAGTGAAGCGCCGGATTCTGGTCGGCACGTACGTGCTGTCGC
ACGGCTACTACGACGCGTACTACCTGCAGGCGCAGAAGATCCGCCGCATCATCGCGCAGGATTTCCAGGAAGCGTTCAAA
TCCTGCGACGTGATCATGGGCCCGGCATCGCCCACCGTCGCGTGGGACATCGGCGCGAAGGGCGACGATCCCGTCCAGAT
GTATCTCGCGGACATCTACACGCTGTCGGTGAGCCTCGCGGGCCTGCCCGGCATGAGCGTGCCGTGCGGCTTCGGCGCGG
GCGCGAACGCGAAGCGCCCGGTCGGCCTGCAGATCATCGGCAACTATTTCGACGAAGCCCGGATGCTGCAGGTCGCCGAC
GCGTTCCAGCGCGCGACCGACTGGCACGTACAAGAACCGGCAGGAGTGTGA

Upstream 100 bases:

>100_bases
GCAGCCGCGCTGCCGTCGGCTCCGCCCGCCCGATTCGCCAGCTTGTTCGCCCGGGCGTGCGCTTTTCGCCGCGCGCCGAA
GAATCCAGGAAACCCACGCA

Downstream 100 bases:

>100_bases
GCATGACCCAGTGGGAAGTCGTTATCGGCCTCGAGACGCACGCGCAACTGTCGACCGTCTCGAAGATTTTCTCGGGCGCG
TCGACGCAGTTCGGCGCGGA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MHAKSLTELRAALDAKECSAVELAQHYLKRIDAARDLNAFVHVDAELTLAQAKAADAALAKGEAGPLAGLPIAHKDVFVT
RGWRSTAGSKMLANYASPFDATVVARLSAAGMVTLGKTNMDEFAMGSSNENSAFGPVKNPWDTNAVPGGSSGGSSAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMARSAADCALLLNAMAGFDERDSTSLERA
DEDYTRHLGKTWAAGGDAGKPLAGLRIGLPAEYFGAGLADDVRAAIDAALKTYETLGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQEAFK
SCDVIMGPASPTVAWDIGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFDEARMLQVAD
AFQRATDWHVQEPAGV

Sequences:

>Translated_496_residues
MHAKSLTELRAALDAKECSAVELAQHYLKRIDAARDLNAFVHVDAELTLAQAKAADAALAKGEAGPLAGLPIAHKDVFVT
RGWRSTAGSKMLANYASPFDATVVARLSAAGMVTLGKTNMDEFAMGSSNENSAFGPVKNPWDTNAVPGGSSGGSSAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMARSAADCALLLNAMAGFDERDSTSLERA
DEDYTRHLGKTWAAGGDAGKPLAGLRIGLPAEYFGAGLADDVRAAIDAALKTYETLGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQEAFK
SCDVIMGPASPTVAWDIGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFDEARMLQVAD
AFQRATDWHVQEPAGV
>Mature_496_residues
MHAKSLTELRAALDAKECSAVELAQHYLKRIDAARDLNAFVHVDAELTLAQAKAADAALAKGEAGPLAGLPIAHKDVFVT
RGWRSTAGSKMLANYASPFDATVVARLSAAGMVTLGKTNMDEFAMGSSNENSAFGPVKNPWDTNAVPGGSSGGSSAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMARSAADCALLLNAMAGFDERDSTSLERA
DEDYTRHLGKTWAAGGDAGKPLAGLRIGLPAEYFGAGLADDVRAAIDAALKTYETLGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQEAFK
SCDVIMGPASPTVAWDIGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFDEARMLQVAD
AFQRATDWHVQEPAGV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=514, Percent_Identity=37.3540856031128, Blast_Score=322, Evalue=7e-88,
Organism=Homo sapiens, GI195972892, Length=503, Percent_Identity=26.441351888668, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI166795287, Length=178, Percent_Identity=36.5168539325843, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=469, Percent_Identity=37.7398720682303, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=514, Percent_Identity=24.124513618677, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=31.25, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=229, Percent_Identity=30.5676855895196, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=236, Percent_Identity=30.5084745762712, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=192, Percent_Identity=33.8541666666667, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=192, Percent_Identity=33.8541666666667, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=202, Percent_Identity=29.2079207920792, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=444, Percent_Identity=34.9099099099099, Blast_Score=249, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=466, Percent_Identity=25.9656652360515, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320448, Length=237, Percent_Identity=28.6919831223629, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648113, Length=491, Percent_Identity=39.5112016293279, Blast_Score=298, Evalue=8e-81,
Organism=Drosophila melanogaster, GI24652985, Length=505, Percent_Identity=26.7326732673267, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI19922090, Length=505, Percent_Identity=26.7326732673267, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652981, Length=505, Percent_Identity=26.7326732673267, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652983, Length=505, Percent_Identity=26.7326732673267, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24644968, Length=505, Percent_Identity=26.3366336633663, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078093, Length=522, Percent_Identity=26.6283524904215, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24648435, Length=235, Percent_Identity=30.6382978723404, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24648437, Length=235, Percent_Identity=30.6382978723404, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI45550774, Length=235, Percent_Identity=30.6382978723404, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21356731, Length=238, Percent_Identity=31.0924369747899, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648441, Length=170, Percent_Identity=34.1176470588235, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648439, Length=170, Percent_Identity=34.1176470588235, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52338; Mature: 52338

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAKSLTELRAALDAKECSAVELAQHYLKRIDAARDLNAFVHVDAELTLAQAKAADAALA
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHH
KGEAGPLAGLPIAHKDVFVTRGWRSTAGSKMLANYASPFDATVVARLSAAGMVTLGKTNM
CCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCEEEECCCCH
DEFAMGSSNENSAFGPVKNPWDTNAVPGGSSGGSSAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCH
FAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMARSAADCALLLNAMAGFDERDSTSLERA
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DEDYTRHLGKTWAAGGDAGKPLAGLRIGLPAEYFGAGLADDVRAAIDAALKTYETLGATL
HHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCEE
VPVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGPE
EEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
VKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQEAFKSCDVIMGPASPTVAWDIGAK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCC
GDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFDEARMLQVAD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AFQRATDWHVQEPAGV
HHHHHCCCCCCCCCCC
>Mature Secondary Structure
MHAKSLTELRAALDAKECSAVELAQHYLKRIDAARDLNAFVHVDAELTLAQAKAADAALA
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHH
KGEAGPLAGLPIAHKDVFVTRGWRSTAGSKMLANYASPFDATVVARLSAAGMVTLGKTNM
CCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCEEEECCCCH
DEFAMGSSNENSAFGPVKNPWDTNAVPGGSSGGSSAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCH
FAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMARSAADCALLLNAMAGFDERDSTSLERA
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DEDYTRHLGKTWAAGGDAGKPLAGLRIGLPAEYFGAGLADDVRAAIDAALKTYETLGATL
HHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCEE
VPVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGPE
EEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
VKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQEAFKSCDVIMGPASPTVAWDIGAK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCC
GDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFDEARMLQVAD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AFQRATDWHVQEPAGV
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA