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The map label for this gene is gatB [H]

Identifier: 83718877

GI number: 83718877

Start: 179911

End: 181383

Strand: Direct

Name: gatB [H]

Synonym: BTH_I0149

Alternate gene names: 83718877

Gene position: 179911-181383 (Clockwise)

Preceding gene: 83721575

Following gene: 83719841

Centisome position: 4.72

GC content: 66.19

Gene sequence:

>1473_bases
ATGACCCAGTGGGAAGTCGTTATCGGCCTCGAGACGCACGCGCAACTGTCGACCGTCTCGAAGATTTTCTCGGGCGCGTC
GACGCAGTTCGGCGCGGAACCCAACACGCAGGCGTGCCCCGTCGATCTCGCGCTGCCGGGCGTGCTGCCCGTGCTGAACC
GCGGCGCGGTCGAGCGGGCGATCCGCTTCGGCCTCGCGATCGGCGCGACGGTGGCGCCGCGCAGCATCTTCGCGCGCAAG
AATTACTTCTATCCGGATCTGCCGAAGGGCTATCAGATCAGCCAGTACGAGATTCCGGTCGTGCAGGGCGGCCAGATCAC
GATCCAGGTGCCCGCCAACGAAAAGGCAGGCAAGCAGGCGTATTCGAAGACGGTCAACCTGACCCGCGCGCACCTCGAAG
AGGACGCGGGCAAATCGCTGCACGAGGACTTCGCGGGGATGACGGGCATCGACCTGAACCGCGCGGGCACGCCGCTGCTC
GAGATCGTCACCGAGCCGGAAATGAGGAGCGCGGCCGAGGCGGTCGCGTACGCGAAGGCGCTGCACGGCCTCGTCGTGTG
GCTCGGCATCTGCGACGGCAACATGCAGGAAGGATCGTTCCGCTGCGACGCGAACGTGTCGGTGCGCCCGGTCGGCCAGG
AGAAGTTCGGCACGCGCGCCGAGATCAAGAACCTGAACTCGTTCCGCTTCCTCGAGGACGCGATCAACTATGAAGTGCGC
CGCCAGATCGAGCTGATCGAGGACGGCGGCGAAGTCGTGCAGGAAACGCGCCTGTACGATCCGGACAAGCGCGAGACGCG
CTCGATGCGCAGCAAGGAAGACGCGCACGACTACCGCTACTTCCCCGATCCGGACCTGATGCCGCTCGTGATCGGCGCGG
ACTGGATCGAGCGCGTGAAGGGCGAGATGCCCGAGCTGCCGGCCGCGATGCAGCAGCGCTTCGTCGAGCAGTACGGCGTG
TCCGCGTACGACGCGGGCGTGCTGACGTCGACGAAGGCGATGGCCGCGTACTTCGAGGCCGTCGTCGCGAAGGCGGGCGC
GGCAAACGCGAAGATCGCCGCGAACTGGCTGATGGGCGACGTGTCGTCGCAACTCAATCGCGACGGCATCGACATCGATG
CGTGCCCGGTGTCGGCCGCGCAGCTCGCGCTCGTGCTGCAGCGGATCGCCGACGGCACGATCTCGAACAAGATCGCGAAG
GAAATCTTCGTGGCGATCTGGGACGAGAAGGCGGCCGACGAAGGCGCGGCCGACCGCATCATCGATGCGAAGGGCCTGAA
GCAGATCTCCGATACGGGCGCGCTCGAGGCGATCATCGACGAGGTGCTCGCCGCGAACGCGAAGTCGGTCGAGGAGTTCC
GCGCGGGCAAGGAAAAGGCGTTCAACGCGCTCGTCGGCCAGGCGATGAAGGCGACGAAGGGCAAGGCGAACCCGCAGCAG
GTCAACGAGCTCCTGAAGAAGAAGCTCGGCTGA

Upstream 100 bases:

>100_bases
TCATCGGCAACTATTTCGACGAAGCCCGGATGCTGCAGGTCGCCGACGCGTTCCAGCGCGCGACCGACTGGCACGTACAA
GAACCGGCAGGAGTGTGAGC

Downstream 100 bases:

>100_bases
GCGGGCGCCGCCGAATTGAAGCCGTACTGATGCAGGCGGGCCGCCCCGAACGCAGGTTCGGCGGCGGCCCGCTCGCGTTT
CGCGGGGCGCCGGGCCGCGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGATVAPRSIFARK
NYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKQAYSKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLL
EIVTEPEMRSAAEAVAYAKALHGLVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEDAINYEVR
RQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGADWIERVKGEMPELPAAMQQRFVEQYGV
SAYDAGVLTSTKAMAAYFEAVVAKAGAANAKIAANWLMGDVSSQLNRDGIDIDACPVSAAQLALVLQRIADGTISNKIAK
EIFVAIWDEKAADEGAADRIIDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPQQ
VNELLKKKLG

Sequences:

>Translated_490_residues
MTQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGATVAPRSIFARK
NYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKQAYSKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLL
EIVTEPEMRSAAEAVAYAKALHGLVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEDAINYEVR
RQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGADWIERVKGEMPELPAAMQQRFVEQYGV
SAYDAGVLTSTKAMAAYFEAVVAKAGAANAKIAANWLMGDVSSQLNRDGIDIDACPVSAAQLALVLQRIADGTISNKIAK
EIFVAIWDEKAADEGAADRIIDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPQQ
VNELLKKKLG
>Mature_489_residues
TQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGATVAPRSIFARKN
YFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKQAYSKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLE
IVTEPEMRSAAEAVAYAKALHGLVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEDAINYEVRR
QIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGADWIERVKGEMPELPAAMQQRFVEQYGVS
AYDAGVLTSTKAMAAYFEAVVAKAGAANAKIAANWLMGDVSSQLNRDGIDIDACPVSAAQLALVLQRIADGTISNKIAKE
IFVAIWDEKAADEGAADRIIDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPQQV
NELLKKKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=39, Blast_Score=358, Evalue=8e-99,
Organism=Caenorhabditis elegans, GI115534094, Length=472, Percent_Identity=32.2033898305085, Blast_Score=230, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6319391, Length=523, Percent_Identity=31.3575525812619, Blast_Score=227, Evalue=3e-60,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=35.8148893360161, Blast_Score=335, Evalue=5e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53262; Mature: 53131

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERA
CCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCHHHHHH
IRFGLAIGATVAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKQA
HHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCHHHHHH
YSKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAKA
HHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
LHGLVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEDAINYEVR
HHHHHEEEEECCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCCHHHHHHHHHCHHHH
RQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGADWIERVK
HHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHC
GEMPELPAAMQQRFVEQYGVSAYDAGVLTSTKAMAAYFEAVVAKAGAANAKIAANWLMGD
CCCCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VSSQLNRDGIDIDACPVSAAQLALVLQRIADGTISNKIAKEIFVAIWDEKAADEGAADRI
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPQQ
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHH
VNELLKKKLG
HHHHHHHHCC
>Mature Secondary Structure 
TQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERA
CCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCHHHHHH
IRFGLAIGATVAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKQA
HHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCHHHHHH
YSKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAKA
HHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
LHGLVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEDAINYEVR
HHHHHEEEEECCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCCHHHHHHHHHCHHHH
RQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGADWIERVK
HHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHC
GEMPELPAAMQQRFVEQYGVSAYDAGVLTSTKAMAAYFEAVVAKAGAANAKIAANWLMGD
CCCCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VSSQLNRDGIDIDACPVSAAQLALVLQRIADGTISNKIAKEIFVAIWDEKAADEGAADRI
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPQQ
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHH
VNELLKKKLG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA