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The map label for this gene is rpoS [H]

Identifier: 83721572

GI number: 83721572

Start: 2502746

End: 2503837

Strand: Direct

Name: rpoS [H]

Synonym: BTH_I2226

Alternate gene names: 83721572

Gene position: 2502746-2503837 (Clockwise)

Preceding gene: 83721288

Following gene: 83720902

Centisome position: 65.7

GC content: 64.29

Gene sequence:

>1092_bases
ATGCCGAAATCGAAGCGCCACGATCCGCAAGCCGAGTCTGAGAAGCTCAGTCGAGCCACGCAAGCATCGGTGGAGCGAAC
GGGTGCTTCGACGGACGAGGACGAAGACGCTGCCGACAGCGAACGCGACTACGAGCCGCGCGAAGCGGATTCTGACGATT
CGCCGGGCGAAGGGCGCGGCGAGACGGCCGACGCGTCGCCCGACCTGGACGACTTCCGGGCGCTTCTGCAGGCGGAACTC
ACCGCCGACACGATTCAGCACTACCTGAACCGCATCAGCGTGAAGCCGCTGTTGACGGTCGAGGAAGAGCAGCGCTATTC
GCGGCTCGCGAAGGCGGGAGAATTCGAGGCGCGGCAGGTGATGATCGAGCGCAACCTTCGGCTCGTCGTCAGCATCGCGA
AAGGCTATCTGAATCGCGGCGTGCCGCTTCTCGATCTGATCGAAGAGGGCAATCTCGGGCTCATGCACGCAATCGAAAAA
TTCGATCCGACGCGCGGCTTTCGTTTTTCGACGTACGCGACCTGGTGGATTCGCCAGAGCATCGAGCGCGCGATCATGAA
TCAGGCGCGCACGGTGCGACTGCCGGTGCACGTGATCCGCGAGTTGAACCAGGTGCTGCGCGCGAAGCGCCATCTCGAGA
AGAACTCGCTGTCGACGGGCGAGGCCGCCGAACGCCGCGAGGCGAGCATCGACGACATCGCGTATCTGACCGGCAAGACG
GCAGAGGAGGTCACCGACATCCTCGCGCTGAACGAGCACACCGCATCGCTCGACGCGCCGCTCGATCTCGATCCGGCGAG
CAGCCTGCTCGATCTTCTACCCGACGACCAGAGCCAGTCGCCCGACGCGGAGGTTCAGCACCGCGAGTTGGAGACGCTCA
CGCGCGCGTGGCTGTCGCGTCTGTCCGACAAGCACCGTCACGTGATCGAGCGGCGCTTCGGCCTCAACCATATCGAACCC
GCGACGCTCGAGGAGCTTGCCGACGAGATGGGACTGACCCGCGAGCGGGTTCGTCAGATCCAGCAGGAAGCGCTCGTGCG
GCTCAAGCGGTTTTTCGCCTCCAACGGCGTGCGCAAGGACGCCGTTCTGTAA

Upstream 100 bases:

>100_bases
ATAGCGATTCCGACCGCGTGATGCTGCATTTCGAGGTTCGCCGGCAGGGTAAACCTGTCGATCCGCTGAAGTATTTGCCG
CCTCAATAAACGAGACGACC

Downstream 100 bases:

>100_bases
CTCATCGATGACACCGATTCTGGTTTTTGACATCGAGACGATTCCCGATGTCGACGGCATTCGCCGTCTTGAAGATCTGC
CCGCCGACCTTGCCGACGCC

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MPKSKRHDPQAESEKLSRATQASVERTGASTDEDEDAADSERDYEPREADSDDSPGEGRGETADASPDLDDFRALLQAEL
TADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEK
FDPTRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSLSTGEAAERREASIDDIAYLTGKT
AEEVTDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSRLSDKHRHVIERRFGLNHIEP
ATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL

Sequences:

>Translated_363_residues
MPKSKRHDPQAESEKLSRATQASVERTGASTDEDEDAADSERDYEPREADSDDSPGEGRGETADASPDLDDFRALLQAEL
TADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEK
FDPTRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSLSTGEAAERREASIDDIAYLTGKT
AEEVTDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSRLSDKHRHVIERRFGLNHIEP
ATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL
>Mature_362_residues
PKSKRHDPQAESEKLSRATQASVERTGASTDEDEDAADSERDYEPREADSDDSPGEGRGETADASPDLDDFRALLQAELT
ADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKF
DPTRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSLSTGEAAERREASIDDIAYLTGKTA
EEVTDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSRLSDKHRHVIERRFGLNHIEPA
TLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=281, Percent_Identity=50.1779359430605, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1789448, Length=264, Percent_Identity=37.8787878787879, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789871, Length=282, Percent_Identity=28.0141843971631, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 41147; Mature: 41016

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSKRHDPQAESEKLSRATQASVERTGASTDEDEDAADSERDYEPREADSDDSPGEGRG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ETADASPDLDDFRALLQAELTADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCHHHHHH
MIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPTRGFRFSTYATWWIRQS
HHHHCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHH
IERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSLSTGEAAERREASIDDIAYLTGKT
HHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHCCCC
AEEVTDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSR
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LSDKHRHVIERRFGLNHIEPATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKD
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
AVL
CCC
>Mature Secondary Structure 
PKSKRHDPQAESEKLSRATQASVERTGASTDEDEDAADSERDYEPREADSDDSPGEGRG
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ETADASPDLDDFRALLQAELTADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCHHHHHH
MIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPTRGFRFSTYATWWIRQS
HHHHCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHH
IERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSLSTGEAAERREASIDDIAYLTGKT
HHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHCCCC
AEEVTDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSR
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LSDKHRHVIERRFGLNHIEPATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKD
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
AVL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]