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The map label for this gene is purR [H]

Identifier: 83721527

GI number: 83721527

Start: 3245624

End: 3246928

Strand: Direct

Name: purR [H]

Synonym: BTH_I2828

Alternate gene names: 83721527

Gene position: 3245624-3246928 (Clockwise)

Preceding gene: 83720762

Following gene: 83720743

Centisome position: 85.2

GC content: 70.96

Gene sequence:

>1305_bases
GTGCGCGTCGCCGACGGAACGAGCGCATGTACTCGACCGTGTCCTCGCATCGCTTTCGCTCATCGGCATCGTCCGAATAC
CGATGCCGCTGCATGTCGCCGATCGCCGCGCCCTTGCCGCATCGCGTGCCCGCCGCCACTCATGCTTCCGACGTCGCCGC
CGTCACCCGCCTTTGCCGCCGTCATCAGTTCTTCATCGACGCCGCCGATAATCCCGTCGACGAAAACGTTTACACGCATC
CGCCTCCCGCTCATGACGCCGACCATCAAAGACGTCGCCGCGCTCGCCGGCTTTTCGATCGCCACCGTGTCGCGCGCGAT
CAACGCGCCGCACACGGTCCATCCCGCGACGCTCGAGAAGATCCGCGCGGCGATCGATGCGCTGCGCTTCCGCCCGAATC
CGCTCGGCCGGCAACTGCGCAGCGACCGCACGCAATTGATCGGCGTCGTGCTGCCGACGCTCGCGAATCCCGTGTTCGCC
GAATGCCTGCAAGGCGTCGACGAGCTCGCGGCGCAAGCCGGCTTCAAGCTGATCGTGATGTCGACCGAATACGACTCGGC
GCGCGAGCGCCATGCGATCGAGACGCTGCGCGCGCAGCGCGTCGAAGGGCTGATGCTGACCGTCGCCGATGCCGACGCGC
ACCCGCTCCTCGACGAGCTGGACCACAACGGCCCGCTCTACGTGCTGATGCACAACGACACGCCGCATCGCCCGTCGGTC
GCGGTCGACAATCGCCGCGCCGCGTACGACGGCGTGCGGATGCTGATCGAGCGCGGCCATCGGCGCGTGCTGATGCTCGC
GGGCTCGCTCGACGCGTCGGACCGCGCGCGGCTGCGCGTGCGAGGCTATGCGCAGGCGCTCGACGAGCACGGGCTCGATC
CGCTGCCCGCGCTCGAGCTCGACTTCAACGCGCCGGCGCTGCCGCACGCGATGCTCGCGCATCTGAGCGCGCGCGCGACG
CGGCCCACCGCGCTCTTCTGCAGCAACGACTGGCTCGCGATGGTCGTGATCCGCGGCCTGCGCGACGCGCGCCTCGCGGT
GCCCGATGACATGTCGGTGCTCGGCTTCGACGGCCTCGCGGTCGGCGAGCTGCTCGCGCCGCCGCTCGCGAGCGTCGCGA
CGCCGAATCGCGAGATCGGCCGCGCCGCATGGCGGCGTCTTGCCGAGCGCATCGCCGGCACGCGTCATGCGCATCCCGCG
CTCACCTTGCCGCACGCGGTGCGCGATGGCGCGACCGTCGCGCCGCCTCGCGAGGCGCACGATGCGCACGACGCGCACGA
TGCGCGCGCGCCGCGCGTCGCCTGA

Upstream 100 bases:

>100_bases
GCTCCAAAACCGGAGATGTCGCGCCGCTGCTCGCGCCGAGGACGCTCGCCGGTCGAGCCGGCGACGTCACGATGAGCGGC
GGATTTTCCGCGTTGCTCGC

Downstream 100 bases:

>100_bases
CACCCGCCTTCGCTTCCCGTCGCATCCACTCGCTTCGCCCATTCAAGGAGACCTGTCGTGACTGTCCGCCGTTCGCCTCT
CTCGCGCTCGCCGCTCGCCG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MRVADGTSACTRPCPRIAFAHRHRPNTDAAACRRSPRPCRIACPPPLMLPTSPPSPAFAAVISSSSTPPIIPSTKTFTRI
RLPLMTPTIKDVAALAGFSIATVSRAINAPHTVHPATLEKIRAAIDALRFRPNPLGRQLRSDRTQLIGVVLPTLANPVFA
ECLQGVDELAAQAGFKLIVMSTEYDSARERHAIETLRAQRVEGLMLTVADADAHPLLDELDHNGPLYVLMHNDTPHRPSV
AVDNRRAAYDGVRMLIERGHRRVLMLAGSLDASDRARLRVRGYAQALDEHGLDPLPALELDFNAPALPHAMLAHLSARAT
RPTALFCSNDWLAMVVIRGLRDARLAVPDDMSVLGFDGLAVGELLAPPLASVATPNREIGRAAWRRLAERIAGTRHAHPA
LTLPHAVRDGATVAPPREAHDAHDAHDARAPRVA

Sequences:

>Translated_434_residues
MRVADGTSACTRPCPRIAFAHRHRPNTDAAACRRSPRPCRIACPPPLMLPTSPPSPAFAAVISSSSTPPIIPSTKTFTRI
RLPLMTPTIKDVAALAGFSIATVSRAINAPHTVHPATLEKIRAAIDALRFRPNPLGRQLRSDRTQLIGVVLPTLANPVFA
ECLQGVDELAAQAGFKLIVMSTEYDSARERHAIETLRAQRVEGLMLTVADADAHPLLDELDHNGPLYVLMHNDTPHRPSV
AVDNRRAAYDGVRMLIERGHRRVLMLAGSLDASDRARLRVRGYAQALDEHGLDPLPALELDFNAPALPHAMLAHLSARAT
RPTALFCSNDWLAMVVIRGLRDARLAVPDDMSVLGFDGLAVGELLAPPLASVATPNREIGRAAWRRLAERIAGTRHAHPA
LTLPHAVRDGATVAPPREAHDAHDAHDARAPRVA
>Mature_434_residues
MRVADGTSACTRPCPRIAFAHRHRPNTDAAACRRSPRPCRIACPPPLMLPTSPPSPAFAAVISSSSTPPIIPSTKTFTRI
RLPLMTPTIKDVAALAGFSIATVSRAINAPHTVHPATLEKIRAAIDALRFRPNPLGRQLRSDRTQLIGVVLPTLANPVFA
ECLQGVDELAAQAGFKLIVMSTEYDSARERHAIETLRAQRVEGLMLTVADADAHPLLDELDHNGPLYVLMHNDTPHRPSV
AVDNRRAAYDGVRMLIERGHRRVLMLAGSLDASDRARLRVRGYAQALDEHGLDPLPALELDFNAPALPHAMLAHLSARAT
RPTALFCSNDWLAMVVIRGLRDARLAVPDDMSVLGFDGLAVGELLAPPLASVATPNREIGRAAWRRLAERIAGTRHAHPA
LTLPHAVRDGATVAPPREAHDAHDAHDARAPRVA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=310, Percent_Identity=31.6129032258064, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1790194, Length=310, Percent_Identity=34.1935483870968, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1790715, Length=344, Percent_Identity=27.906976744186, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI48994940, Length=331, Percent_Identity=30.8157099697885, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=31.864406779661, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=31.1377245508982, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=30.3571428571429, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789068, Length=340, Percent_Identity=30, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786540, Length=338, Percent_Identity=29.2899408284024, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=27.7258566978193, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787906, Length=319, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790689, Length=308, Percent_Identity=23.3766233766234, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786268, Length=332, Percent_Identity=24.3975903614458, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 46965; Mature: 46965

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVADGTSACTRPCPRIAFAHRHRPNTDAAACRRSPRPCRIACPPPLMLPTSPPSPAFAA
CCCCCCCCHHCCCCCHHHHHHCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHHH
VISSSSTPPIIPSTKTFTRIRLPLMTPTIKDVAALAGFSIATVSRAINAPHTVHPATLEK
HHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHH
IRAAIDALRFRPNPLGRQLRSDRTQLIGVVLPTLANPVFAECLQGVDELAAQAGFKLIVM
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEE
STEYDSARERHAIETLRAQRVEGLMLTVADADAHPLLDELDHNGPLYVLMHNDTPHRPSV
ECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCE
AVDNRRAAYDGVRMLIERGHRRVLMLAGSLDASDRARLRVRGYAQALDEHGLDPLPALEL
EECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHEEHHHHHHHHHHCCCCCCCCEEE
DFNAPALPHAMLAHLSARATRPTALFCSNDWLAMVVIRGLRDARLAVPDDMSVLGFDGLA
CCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCEECCCCHH
VGELLAPPLASVATPNREIGRAAWRRLAERIAGTRHAHPALTLPHAVRDGATVAPPREAH
HHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCHHC
DAHDAHDARAPRVA
CCCCHHHCCCCCCC
>Mature Secondary Structure
MRVADGTSACTRPCPRIAFAHRHRPNTDAAACRRSPRPCRIACPPPLMLPTSPPSPAFAA
CCCCCCCCHHCCCCCHHHHHHCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHHH
VISSSSTPPIIPSTKTFTRIRLPLMTPTIKDVAALAGFSIATVSRAINAPHTVHPATLEK
HHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHH
IRAAIDALRFRPNPLGRQLRSDRTQLIGVVLPTLANPVFAECLQGVDELAAQAGFKLIVM
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEE
STEYDSARERHAIETLRAQRVEGLMLTVADADAHPLLDELDHNGPLYVLMHNDTPHRPSV
ECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCE
AVDNRRAAYDGVRMLIERGHRRVLMLAGSLDASDRARLRVRGYAQALDEHGLDPLPALEL
EECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHEEHHHHHHHHHHCCCCCCCCEEE
DFNAPALPHAMLAHLSARATRPTALFCSNDWLAMVVIRGLRDARLAVPDDMSVLGFDGLA
CCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCEECCCCHH
VGELLAPPLASVATPNREIGRAAWRRLAERIAGTRHAHPALTLPHAVRDGATVAPPREAH
HHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCHHC
DAHDAHDARAPRVA
CCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA