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The map label for this gene is gcl [H]

Identifier: 83721511

GI number: 83721511

Start: 2447822

End: 2449597

Strand: Reverse

Name: gcl [H]

Synonym: BTH_I2173

Alternate gene names: 83721511

Gene position: 2449597-2447822 (Counterclockwise)

Preceding gene: 83719159

Following gene: 83721098

Centisome position: 64.31

GC content: 67.74

Gene sequence:

>1776_bases
ATGGCCAGGATGAGAGCCGTCGACGCAGCCGTGCTCGTACTCGAGAGGGAAGGCGTGCAGACCGCGTTCGGCGTGCCGGG
CGCCGCGATCAACCCGCTGTATTCGGCGCTGCGTCGCTCGGGCGCGATCGGGCACGTGCTCGCGCGGCACGTCGAAGGCG
CGTCGCACATGGCGGAAGGCTACACGCGGGCCGCGCCCGGCAACATCGGCGTGTGCATCGGCACGTCGGGCCCCGCCGGC
ACCGACATGATCACGGGCCTGTACTCCGCGTCGGCCGACTCGATCCCGATCCTCGCGATCACCGGCCAGGCGCCGCGCGC
GCGGCTCTACAAGGAAGACTTCCAGGCGGTCGACATCGAATCGATCGCGAAGCCCGTCACGAAATGGGCGGTCACGGTGC
GCGAGCCGGCGCTCGTGCCGCGCGTGTTCCAGCAGGCGTTCCACCTGATGCGATCGGGCCGCCCGGGCCCCGTGCTGATC
GATCTGCCGATCGACGTGCAACTCGCCGAGATCGAATTCGATATCGCGACCTACGAGCCGCTGCCCGTCTACAAGCCGCG
CGCGACGCGCGCACAGATCGAGGCCGCGCTCGCGATGTTGAACGACGCCGAGCGCCCGCTCATCGTGTCGGGCGGCGGCG
TGATCAACGCGGCGGCCGAGCATCTGCTCGTCGAATTCGCGCAGACACTCGGCGTGCCGGTAATCCCGACGCTGATGTCG
TGGGGCGCGATTCCCGACGATCACCCGCTGATGGCCGGCATGGTCGGGCTGCAGACGTCGCACCGCTACGGCAACGCAAC
GCTCCTCGCATCCGATTTCGTGCTCGGCATCGGCAACCGCTGGGCGAACCGCCACACCGGCAGCGTCGACGTCTACACGA
AGGGCCGCAAGTTCGTTCACGTCGACATCGAACCGACGCAGATCGGCCGCGTGTTCGGCCCCGATCTCGGGATCGTGTCG
GATGCGAAGGCGGCGCTCGAGCTGTTTGTCGAGATCGCGCGCGAATGGAAGGCCGCGGGCAAGCTGAAGGACCGCGGCGC
GTGGGTCGCCGATTGCCAGCAGCGCAAGCGCACGCTGCAGCGCAAGACGCATTTCGACGACGTGCCCGTCAAGCCGCAGC
GCGTATACGAAGAGATGAACAAGGCGTTCGGCCGCGATACATGCTACGTGAGCACGATCGGGCTGTCGCAGATCGCGGCC
GCGCAGTTCCTGCACGTGTTCAAGGCGCGCAACTGGATCAACTGCGGCCAGGCGGGCCCGCTCGGCTGGACGATTCCCGC
CGCGCTCGGCGTGCGCGCGGCCGATCCTCAGCGCCCGATCGTCGCGCTGTCGGGCGACTACGACTTCCAGTTCATGATCG
AGGAGCTCGCCGTCGGCGCGCAGTTCAAGCTGCCGTACGTGCACATCGTCGTCAACAACTCGTACCTCGGCCTGATCCGT
CAGGCGCAGCGCGCGTTCGACATGGACTACTGCGTGCAGCTCGCGTTCGACAACATCAACGCGCCCGAGGTGAACGGCTA
TGGCGTCGATCATGTCGCCGTCGCCGAAGGGCTCGGCTGCAAGGCGCTGCGCGTGTTCAAGCCCGAGGAGATCGCGCCCG
CGCTCGAGCGCGCCCAACGGATGACGGCCGAGTTCAGCGTGCCCGTCGTCGTCGAGGTGATTCTCGAGCGCGTGACGAAC
ATCTCGATGGGCGCCGAGATCGACGCGATCAACGAGTTCGAGGATCTCGCGGACCGCGCCGAGCATGCGCCGACCGCGAT
CTCGCTGCTCGACTGA

Upstream 100 bases:

>100_bases
ATAAGCGACTCGGCTCGATCTTATCAAACCTTTCCTGCATCCCTAAAGTGTCCTCGACACCAGCGATACCCGCGATTCGC
ATCGAACAAGGAGACATTTC

Downstream 100 bases:

>100_bases
CGCGCCTCGCGCCCATCGCTTCGCCTGCTTGATTGATCAGCATCGCTGATCAGCTTGACCGCTTCGACTTGCACAACCGA
CCGACCAGCGAGATGACCGA

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MARMRAVDAAVLVLEREGVQTAFGVPGAAINPLYSALRRSGAIGHVLARHVEGASHMAEGYTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLI
DLPIDVQLAEIEFDIATYEPLPVYKPRATRAQIEAALAMLNDAERPLIVSGGGVINAAAEHLLVEFAQTLGVPVIPTLMS
WGAIPDDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVDVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVEIAREWKAAGKLKDRGAWVADCQQRKRTLQRKTHFDDVPVKPQRVYEEMNKAFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAVGAQFKLPYVHIVVNNSYLGLIR
QAQRAFDMDYCVQLAFDNINAPEVNGYGVDHVAVAEGLGCKALRVFKPEEIAPALERAQRMTAEFSVPVVVEVILERVTN
ISMGAEIDAINEFEDLADRAEHAPTAISLLD

Sequences:

>Translated_591_residues
MARMRAVDAAVLVLEREGVQTAFGVPGAAINPLYSALRRSGAIGHVLARHVEGASHMAEGYTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLI
DLPIDVQLAEIEFDIATYEPLPVYKPRATRAQIEAALAMLNDAERPLIVSGGGVINAAAEHLLVEFAQTLGVPVIPTLMS
WGAIPDDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVDVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVEIAREWKAAGKLKDRGAWVADCQQRKRTLQRKTHFDDVPVKPQRVYEEMNKAFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAVGAQFKLPYVHIVVNNSYLGLIR
QAQRAFDMDYCVQLAFDNINAPEVNGYGVDHVAVAEGLGCKALRVFKPEEIAPALERAQRMTAEFSVPVVVEVILERVTN
ISMGAEIDAINEFEDLADRAEHAPTAISLLD
>Mature_590_residues
ARMRAVDAAVLVLEREGVQTAFGVPGAAINPLYSALRRSGAIGHVLARHVEGASHMAEGYTRAAPGNIGVCIGTSGPAGT
DMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLID
LPIDVQLAEIEFDIATYEPLPVYKPRATRAQIEAALAMLNDAERPLIVSGGGVINAAAEHLLVEFAQTLGVPVIPTLMSW
GAIPDDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVDVYTKGRKFVHVDIEPTQIGRVFGPDLGIVSD
AKAALELFVEIAREWKAAGKLKDRGAWVADCQQRKRTLQRKTHFDDVPVKPQRVYEEMNKAFGRDTCYVSTIGLSQIAAA
QFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAVGAQFKLPYVHIVVNNSYLGLIRQ
AQRAFDMDYCVQLAFDNINAPEVNGYGVDHVAVAEGLGCKALRVFKPEEIAPALERAQRMTAEFSVPVVVEVILERVTNI
SMGAEIDAINEFEDLADRAEHAPTAISLLD

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=22.9537366548043, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI21361361, Length=598, Percent_Identity=23.9130434782609, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI1786717, Length=590, Percent_Identity=78.8135593220339, Blast_Score=976, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=544, Percent_Identity=33.8235294117647, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1790104, Length=490, Percent_Identity=33.8775510204082, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=26.6787658802178, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1788716, Length=475, Percent_Identity=25.0526315789474, Blast_Score=129, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17542570, Length=545, Percent_Identity=24.4036697247706, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17531299, Length=557, Percent_Identity=22.6211849192101, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17531301, Length=545, Percent_Identity=22.9357798165138, Blast_Score=96, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=601, Percent_Identity=29.783693843594, Blast_Score=255, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6320816, Length=564, Percent_Identity=24.113475177305, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=496, Percent_Identity=21.1693548387097, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=26.048951048951, Blast_Score=148, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64123; Mature: 63992

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARMRAVDAAVLVLEREGVQTAFGVPGAAINPLYSALRRSGAIGHVLARHVEGASHMAEG
CCCHHHHHHEEEEEECCCCCHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHC
YTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPIDVQLAEIEFDIATYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEEEECCCC
LPVYKPRATRAQIEAALAMLNDAERPLIVSGGGVINAAAEHLLVEFAQTLGVPVIPTLMS
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIPDDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVDVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVEIAREWKAAGKLKDRGAWVADCQQRKRTLQ
EECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RKTHFDDVPVKPQRVYEEMNKAFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAVGAQFKLPYVHIVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEECCEEEEEECCCHHHHHH
QAQRAFDMDYCVQLAFDNINAPEVNGYGVDHVAVAEGLGCKALRVFKPEEIAPALERAQR
HHHHHHCHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHH
MTAEFSVPVVVEVILERVTNISMGAEIDAINEFEDLADRAEHAPTAISLLD
HHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
ARMRAVDAAVLVLEREGVQTAFGVPGAAINPLYSALRRSGAIGHVLARHVEGASHMAEG
CCHHHHHHEEEEEECCCCCHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHC
YTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPIDVQLAEIEFDIATYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEEEECCCC
LPVYKPRATRAQIEAALAMLNDAERPLIVSGGGVINAAAEHLLVEFAQTLGVPVIPTLMS
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIPDDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVDVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVEIAREWKAAGKLKDRGAWVADCQQRKRTLQ
EECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RKTHFDDVPVKPQRVYEEMNKAFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPQRPIVALSGDYDFQFMIEELAVGAQFKLPYVHIVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEECCEEEEEECCCHHHHHH
QAQRAFDMDYCVQLAFDNINAPEVNGYGVDHVAVAEGLGCKALRVFKPEEIAPALERAQR
HHHHHHCHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHH
MTAEFSVPVVVEVILERVTNISMGAEIDAINEFEDLADRAEHAPTAISLLD
HHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]